Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,049,991	Δ12 bp	noncoding (93‑104/109 nt)	spf →	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4049990	1 (0.030)	46 (1.210)	42/282	0.0	97.9%	noncoding (92/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
	?	NC_000913	4050003 =	NA (NA)					noncoding (105/109 nt)	spf	Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

AATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4049848‑4049990 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTC  >  NC_000913/4050003‑4050144                                                                                                                                                                                                                                                                                               AATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAT                                                                                                                                                <  1:248368/143‑1    TACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTT                                                                                                                                             <  2:227316/143‑1       AAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCATTCAGTAATTTTTct                                                                                                                                          >  2:541952/1‑141        AAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATT                                                                                                                                         <  2:359036/143‑1           AGTGGTTTCTGAAGCGAACAAAAAAGAGGAAAGGGAGTCGCGGAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGG                                                                                                                                      <  1:150880/143‑1                   CTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGAGAAAGAA                                                                                                                              >  1:467310/1‑143                   CTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATATATATAGGGCGAAAAAA                                                                                                                              >  1:573614/1‑143                     gaaccaAACAAGAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAA                                                                                                                            <  1:226017/137‑1                        CTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGAT                                                                                                                         >  2:482517/1‑143                         TGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGGATGAACAGATC                                                                                                                        >  1:673249/1‑143                          GAACAAAAAAGAGTTAAGTTAGTCGCGTATTTTATAGAGTTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCC                                                                                                                       <  1:272506/143‑1                             CAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTACTTTTTATTGGGCGAAAGAAAAGATCCGTA                                                                                                                    >  1:168109/1‑143                             CAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTATAGAAGCAAAAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTA                                                                                                                    >  1:190281/1‑143                                     AGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAAGGCCTGA                                                                                                            <  2:472596/143‑1                                             TAGTCGCGTAGGGTACAGAGGGAAGATGTTCTATCTTTTAGGGCTTGTATTTCACGTGAGCGGATTTGGCTGGGTATTTTGGCGGCCGCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTAT                                                                                                    >  1:705168/1‑143                                                 CGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTT                                                                                                <  2:460352/143‑1                                                   CGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTTTTGTGCGAAAGAAAAGATCCGTAATGCCTGATACGCTATGTTTAT                                                                                              >  1:121868/1‑143                                                   CGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATATTAGCCGCCCCAGTCAGTAATTTTTACTGGGCGAAAGAAAAGATCCGTAATGCGTGATTCGATATGTTTAT                                                                                              >  2:456488/1‑143                                                     TAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTATTTTTTTTTTGTCTTATGAAAAGATCCATAATGCCTGATGCGCTATATTTATaa                                                                                            >  1:55177/1‑141                                                      ttgtTACAGTGGTTATATGTTCTATCCTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAG                                                                                           <  1:452976/139‑1                                                             AGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACG                                                                                    >  1:381979/1‑143                                                                   aattttTTCTATCTTTCATATCTTGTACTTCACGGAATCGGAATTGGCTGAATAGTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAA                                                                              <  1:563083/137‑1                                                                              TCTTTCATACTTTTTTCTTGCCGTATGCGGGTGTGGTTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTAT                                                                   <  2:722379/143‑1                                                                              TCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCTAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGTTATGTTTATCAGAGCAACGGTAGAATTGTAAacgcg                                                                   >  2:270064/1‑138                                                                                 TTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGTTGCGCTATGTTTATAAGGACAACGGTAGAATTGTAATCTATTGA                                                                >  1:86413/1‑143                                                                                      ACCTTTTCCTTCTCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTA                                                           <  2:409074/143‑1                                                                                      ACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCGTCGGTCGAATTGTAATCTATTGAATTTA                                                           >  1:293679/1‑143                                                                                       CCTGTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTAC                                                          <  2:574678/143‑1                                                                                        CTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGCTGCGCTATGTTTATGAGGCCAACGGTAAAATTGTAATCTATTGAATTTACa                                                         >  1:581850/1‑142                                                                                              CTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGG                                                   >  2:559067/1‑143                                                                                                 CACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTTTGTTTATCAGGCCTACGGTAGATTTGTCATTTATAGAAATTACGAGCCGGATA                                                >  1:240678/1‑143                                                                                                 CACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATA                                                >  2:396935/1‑143                                                                                                  ACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATAC                                               >  1:567099/1‑143                                                                                                   CGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGCTGCGCTATGTTTATCCGGCCAACGGTAGAATTGTAATCTAATGAATTTACGGGCCGGATACG                                              >  1:425100/1‑143                                                                                                    tTAACCGGATTTGTCTGAATATTTTAGCCGTCACAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGC                                             <  1:643214/142‑1                                                                                                      AATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACCGACCGGATACGACA                                           >  2:364664/1‑143                                                                                                          GGATTTGGCTAAATATGTTAGCCGCCCCATTCAGTAATTTTTATTGTGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTGTATCATGCCAACAGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATC                                       <  2:329866/143‑1                                                                                                             TTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGG                                    <  1:732089/143‑1                                                                                                                     AATATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGACGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGACCAACGGAAGAGTTGTAAACTATGAAATTTACGGGCCGGATACGCGACCACCGGCACAAaga                            >  2:551963/1‑140                                                                                                                       TATTTTAGCCGCCCCAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTCTTGACTTTACGGGCCGGCTACGCCACATCCGGCACAAGCATT                          >  1:148787/1‑143                                                                                                                          TTTAGCCTCCCCAGTCAGTGATTTTTCTTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAG                       <  2:259779/143‑1                                                                                                                                  CCCCAGTCAGTAATTTTTATTAGGCGAAAGAAAAGATCTGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGAGCCGGATACGCCACATCAGGCACAAGCATTACGTCAAGAAA               >  2:85346/1‑143                                                                                                                                     CAGTCAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACCAGCCTTAAGGCAAGAACATT            >  1:633051/1‑143                                                                                                                                         CAGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTC        >  2:21116/1‑143                                                                                                                                          AGTAATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTACTCTATTGAATTTACGGGCCGGATACGCAAAATCCGGCAAAAGCATTAAGGCAAGAAAATTATTaa       >  1:147462/1‑141                                                                                                                                             AATTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCG    >  2:534334/1‑143                                                                                                                                              ATTTTTATTGGGCGCAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAACATTAAGGCAAGAAAATTATTCGCCGT   >  2:458666/1‑143                                                                                                                                               TTTTTATTGGTAGATAGAAAAGATGCGTAATGCCGGATGCGCTGTGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTC  <  1:79286/143‑1                                                                                                                                                                                                                                                                                               AATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4049848‑4049990 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATTCGCCGTC  >  NC_000913/4050003‑4050144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.