New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3394456 =31 (0.810)3 (0.080) 3/270 5.3 9.2% coding (1803/3801 nt) yhdP DUF3971‑AsmA2 domains protein
?NC_000913 3394495 = 30 (0.820)coding (1764/3801 nt) yhdP DUF3971‑AsmA2 domains protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTACCCGGACCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACTGACCTTCGTT  >  NC_000913/3394322‑3394596
                                                                                                                                      |                                                                                                                                             
tCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTACCCGGACCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCCCTGCGGTAAGATTACTCGCCCGCACCCCGCTCCGCTTAACGcg                                                                                                                                       >  1:518033/1‑142 (MQ=255)
             gctgaccaAAGTATTGGCCAACGGCTTTACCCGGACCTTTAAGGTCAGCGTCAATCAGCAGTTTTTGTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCGTCGGTTTTCATc                                                                                                                          <  2:319955/137‑1 (MQ=255)
                      aGTAAGGGCCAACGGCTTTACCCGGACCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACcg                                                                                                                 <  1:167074/143‑1 (MQ=255)
                        tAATGGCCAACGGTTTTACCTGTTCCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTGTGAGGAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACcgtc                                                                                                               <  1:469786/143‑1 (MQ=255)
                        tAAGGGCCAACGGCTTAACGCGGACCATTAAAGACAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACcgtc                                                                                                               <  1:120946/143‑1 (MQ=255)
                                aaCGGCTTTACCCGGACCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTtaataa                                                                                                       >  1:307013/1‑143 (MQ=255)
                                             ggttcTTTAATGTCAGTGTTTATCTTCCGTTTTTCTTTTGAGTAGGCAGGGAGCACGGCGGTAAGACTACGCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAat                                                                                          <  1:80477/139‑1 (MQ=255)
                                               aCCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTATTCATCCAGAAACCGTCGTTCATAAAGCCCAGTTCAatat                                                                                        >  2:374571/1‑143 (MQ=255)
                                                  tttAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGTGATCACTGCGGTAAGATTACTCGCGCGCAAGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAAACGTCGTGAATAAAATTCAACTAAATATCaa                                                                                    >  2:191709/1‑143 (MQ=255)
                                                  tttAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTCATATCGTCCAGTTCAATATCaa                                                                                    >  1:419865/1‑143 (MQ=255)
                                                       tGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATCAACCGTCGTTAATACAGTCGAGTTCCATATCACGgcta                                                                               >  2:655788/1‑143 (MQ=255)
                                                       tGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAACCCGTCGTTAATACACTCCCGTTCAATATCACGgttg                                                                               >  1:120993/1‑142 (MQ=255)
                                                        gTCAGCGTCAATCAGCAGTTTTACTTTTGAGTAGTCAGGGATCACGGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGgttag                                                                              <  1:355946/143‑1 (MQ=255)
                                                             cGTCAATCAGAAGAATTTCTTTTGAGTAGTCAGGGATCACTGCGGTCAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGCTAATAAAATCCCGTTCAATATCCACgttagttagt                                                                         >  2:88505/1‑141 (MQ=255)
                                                              gTCTATCGGCAGTTTTTCTTGTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATg                                                                        <  2:423700/143‑1 (MQ=255)
                                                                          tttttCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTACTAAAGTCCAGTTCAATATCAAGGTTAGCTAATGCAGGCCAGTCCg                                                            >  2:388517/1‑143 (MQ=255)
                                                                             ttcttcTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTCATAAAGTCCACTTCAATATCAAGGATAGTTAATGCAGGCCAGTCCGGCt                                                         >  1:434641/1‑143 (MQ=255)
                                                                                            aGGGATCACTGAGGTAAGAATACTCGCGCGCACGCCGCACAGATTAATGCCATGGGGTTTCATCAATAAACCGTCGTTAATAAAGTCCAGTTCAATATAAAGGTTAGTTAATGCAGGGGAGTCCGGATGGAAGGCAAAATTcg                                          >  2:690715/1‑143 (MQ=255)
                                                                                              ggttCACTGCTGTAAGATTACTCTCGCGCACGCCGCCTAGATTAACGCCAGCGGGTATCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTcgcg                                        <  1:426212/140‑1 (MQ=255)
                                                                                                     tGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGGCCAGTTCAATATCAAGGTTAGTTAATGCACGCCAGTCCGGCTGGAAGGCAAAATTCGCGGTgcgcg                                 >  2:458856/1‑142 (MQ=255)
                                                                                                       cGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCATGGTTAGTTAATGCGTGCCAGTCCTGCTGGAAGGCAAACTTCGCGTTGCGCAGc                               >  1:30770/1‑143 (MQ=255)
                                                                                                            aGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCAAGTCCGGCTGGAAAGCAAACATCGCGTTGCGCAGCGGcgc                          >  2:385248/1‑141 (MQ=255)
                                                                                                              aTTACTCGCGCGCACGCCACCCAGATTAACGCCATCGGGTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATGCAGGATAGTTAATGCACGCCAGTCCGGCTGGAAGGCACACTGCGCGTTACGCAGCGGCagcc                        >  2:346603/1‑140 (MQ=255)
                                                                                                                tACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTTGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGc                      >  1:378717/1‑143 (MQ=255)
                                                                                                                         gcACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGACCAGATCAATATCAAGGGTAGCTAATGCAGGCCAGTCAGGCTGGAAGGCAAACTTGCCGTTGCGCAGCGGCACCCGCCCTACCAAc             >  2:665843/1‑143 (MQ=255)
                                                                                                                          cACGCCGCCTAGATTAACGCCATCGGTTTTCATCTATAAATCGTCGTGAATAAAGTCCAGTACAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACt            <  1:407969/143‑1 (MQ=255)
                                                                                                                           aCGCCGCCCAGATTAACGCCATCGGTGTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCACGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTCGCGCAGCGGCACCAGCCCTTCAAACTg           >  2:728883/1‑143 (MQ=255)
                                                                                                                            cgccgcCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGAAAAGTAGACGTTTCTCAGCGGCACCAGCACTTCAAACTGa          <  2:398033/143‑1 (MQ=255)
                                                                                                                                  ccAGATTAACGCCATCGGTTTTCATCCATATACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGACAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACTGACCTTCg    <  1:33020/143‑1 (MQ=255)
                                                                                                                                   cataTTAACGCCATCGGTTTTCATCCATAAACCGTGGCTAATAAAGTCCAGTTCAAGATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACTGACCTTCGt   <  1:645435/140‑1 (MQ=255)
                                                                                                                                   cAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACTGACCTTCGt   <  1:710469/143‑1 (MQ=255)
                                                                                                                                    aGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGCACTGATAAGACAACATGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACTGACCTTCGtt  <  1:727015/143‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                             
TCTTTCAGCGGTGTCTCATCAAAGTAAGGGCCAACGGCTTTACCCGGACCTTTAATGTCAGCGTCAATCAGCAGTTTTTCTTTTGAGTAGTCAGGGATCACTGCGGTAAGATTACTCGCGCGCACGCCGCCCAGATTAACGCCATCGGTTTTCATCCATAAACCGTCGTTAATAAAGTCCAGTTCAATATCAAGGTTAGTTAATGCAGGCCAGTCCGGCTGGAAGGCAAACTTCGCGTTGCGCAGCGGCACCAGCACTTCAAACTGACCTTCGTT  >  NC_000913/3394322‑3394596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.