New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2840867 =	35 (0.910)	3 (0.080)	3/268	5.2	8.2%	coding (1344/1458 nt)	ascF	cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
	?	NC_000913	2840899 =	34 (0.940)					coding (1376/1458 nt)	ascF	cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAC  >  NC_000913/2840759‑2841040                                                                                                                                             |                                                                                                                                              aaTGGCGGCCGCGGGGCTATTTCCCAGCGTTCAGTGCTTTGTTCCGGCGAATCCAATGAGCGTCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTc                                                                                                                                             <  2:68096/143‑1 (MQ=255)      cAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTCAGGATATTCCTGtt                                                                                                                                        >  2:20577/1‑143 (MQ=255)        gcgcCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGAGGTGCTGTCGTTTATCATCACACTGTTGCTCGGCTTTGCGGATCTTCCTGGTGa                                                                                                                                      >  2:388352/1‑143 (MQ=255)               ggCTATTTAGTAGTGTGCATTTCTTTTTTCCGTTTAATTCTTTGAGTATCGTCTGGGTGTTCGCGGGCAGGGCGCGGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCg                                                                                                                               <  1:402094/143‑1 (MQ=255)                gctgcttACCGGCGCGCAGTTCGTCGCTCCGGCGACTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCgg                                                                                                                              <  1:390880/138‑1 (MQ=255)                  tATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCCGCTTTGAGGATATTCCTGTTGAGGACGCGGCt                                                                                                                            >  1:270218/1‑143 (MQ=255)                    tttACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCCCCCTGTTGCTCCGCTTTGCGGATATTCATGTTGAATAAGCGGATGc                                                                                                                          >  1:438491/1‑143 (MQ=255)                     ttACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCGGGCGGTGGTGCTAGCGTTTATCCTGACACTGTTGCTCGCCTAGGAGGCTAGTCCGGCTGAGGAGACAGCTGca                                                                                                                         >  2:415308/1‑142 (MQ=255)                               gCAGTTCTTCGATCCGGCGCATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGaa                                                                                                               <  2:523444/143‑1 (MQ=255)                                cAGTTTTTTGTTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGAATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGaaa                                                                                                              <  1:644901/143‑1 (MQ=255)                                          aTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGc                                                                                                    <  1:400105/143‑1 (MQ=255)                                                   aTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGACCAGACGCAAAAGTATCACAGCGTAAAacgg                                                                                           >  1:354343/1‑141 (MQ=255)                                                    gccAATGGGCATCGTCGGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAaccga                                                                                          <  1:538415/142‑1 (MQ=255)                                                     ccAATGTGCTTCGTTTGGGTGTTCGCGGTTATGGCGCTGGCGGTGGTGCTGTCGTGTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAaccgac                                                                                         <  1:717506/143‑1 (MQ=255)                                                       aaTGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGAGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGGAGCGGCTGCCCACGCGCGAAATTATCAGAGCGTACAaccgaccg                                                                                       >  2:613270/1‑143 (MQ=255)                                                             cATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGt                                                                                                  >  2:662026/1‑126 (MQ=255)                                                             cATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGt                                                                                                  <  1:662026/126‑1 (MQ=255)                                                                cGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACGGTTGCTCGGCTTTGCGGATATTCCTGTTGAGGAAGCAGCAGCACAGACGAGAAAGTATCAGAGCGTACAACCGACCGTCGCCGaag                                                                              >  1:545356/1‑143 (MQ=255)                                                                     gggTGTTCGCGGTCATGGCGCTGGCGGAGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAAGAAGCGGCTGACCACACGCGAAAGTGTAAGAACTTACAACCGAACGTCGCCAAAGaagta                                                                         >  2:344795/1‑143 (MQ=255)                                                                                 ttctGGGGCTGGCGGTGGTGCTGTCGTTTATCCTCTCACTTTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCTAAAGAAGTAAGTCTTAATTGa                                                             <  2:566924/140‑1 (MQ=255)                                                                                    tGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTGTCAGCGCGTACACCCGCCCGTCGCCAAAGAAGTAAGTCATACATGAGGa                                                          >  1:82364/1‑143 (MQ=255)                                                                                       cgcTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTGTCAGAGCGTGCTGCCGGCCGTCGCCGTAGGTGTGTGTCCTATTTGAGGATGa                                                       >  1:33/1‑143 (MQ=255)                                                                                           ggcgagtgtgCTGTTGTTTATCTTAACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAGCCGACCGTCGCCAAAGAAGTAAGTCATAATTGAGGATGAAAAt                                                   <  2:29095/136‑1 (MQ=255)                                                                                               ggggggCTGTCCTTTATCCTCCCCCTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAGGCGGCTGCCCAGGCGCGAAAGTATTAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAACTGTCa                                               <  2:356152/138‑1 (MQ=255)                                                                                                tgttgttGTCGTTTATCCTTTCACTGTTTCTTGGTTTTGAGGATATTGCGGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAg                                              <  1:20577/137‑1 (MQ=255)                                                                                                      tggcGTTTATCCGCGCGCTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGAGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTc                                        <  2:476316/140‑1 (MQ=255)                                                                                                       gTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGGAAGTCTAAATGGAGGATGAAAATGTCAGCATATcc                                       >  2:67367/1‑143 (MQ=255)                                                                                                          gTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAGGGAGATCAACTTAAATGAGGATGAAAATGTCAGTATTTCCAGa                                    >  2:315704/1‑143 (MQ=255)                                                                                                          gTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGGAGCAGGTCCGCACTGAGGAGCAAAAGGCCATGATTTCCAGa                                    >  2:168983/1‑143 (MQ=255)                                                                                                                  tCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATAGAGGAAGAAAATGTCAGTATTTACAGAAAGttttt                            >  1:581838/1‑143 (MQ=255)                                                                                                                    acacTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTCAATTGAGGATGAAAATGACAGTATATCCAGAAAGTTTTTTa                          >  2:469154/1‑143 (MQ=255)                                                                                                                        tGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTAAGTATTTCCAGAAAGTTTTTTATggg                      >  1:442951/1‑143 (MQ=255)                                                                                                                             cTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCCACGCAGTAAGACTTAATTGAGGAAGAAAATATAAGTATTTCCAGAAAGTTTTTTATGGggcggc                 >  2:148362/1‑143 (MQ=255)                                                                                                                                   tttGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCGGAAAGTTTTTTATGGGGCGGCGCGCtt           >  1:715470/1‑143 (MQ=255)                                                                                                                                      gAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTCAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgcc        >  2:292412/1‑143 (MQ=255)                                                                                                                                          atatTGCTGTTGATGCAGCGGCTGCCCTGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCa    <  2:171371/143‑1 (MQ=255)                                                                                                                                            atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACGGACCGGCGCCAAAGAAGTAAGTCTTAAACGAGGATGAAAATGTCAGTATTTCCAGAAAGTGTGTTGTGGGGCGGCGCGCTTGCCGCAAAc  >  2:430385/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                              AATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAC  >  NC_000913/2840759‑2841040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.