Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,194,572 (T)6→5 intergenic (+47/+151) yqiK → / ← sibD PHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,194,5670T.96.6% 211.6 / 2.5 61intergenic (+42/+156)yqiK/sibDPHB family membrane protein, function unknown/sRNA antisense regulator of toxic IbsD protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base . (31/26);  minor base A (0/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAG  >  NC_000913/3194426‑3194705
                                                                                                                                             |                                                                                                                                           
tGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAAGAACTCCCGTCGAAGAAAAAGCAGCGTAAACCCTCTTCCAGCACTCATCCCTCGCCCAAAGAGCCGGGGAtt                                                                                                                                            >  2:806838/1‑143 (MQ=255)
  aCTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttt                                                                                                                                          >  2:1098230/1‑143 (MQ=255)
     ccTTGCGCAATGAAATTGGCGTTGCAGGCGGCTCACTGCCGGCATTGACTTCACCCTTAACCTCAACAACACCCGTCGAATAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttat                                                                                                                                       <  2:1219140/143‑3 (MQ=255)
      cTTGCTCAATGAAATTGGAGTTTAAGGCGGCTCACGCCCGACACTCCATTGAACGTTAGGCTGAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGACGGGGAttggtata                                                                                                                                      >  1:290482/1‑137 (MQ=255)
          cTCAATGAAATTGGCGTTTCAGGCGGCTCACCGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTg                                                                                                                                 <  2:512766/143‑1 (MQ=255)
           tCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGc                                                                                                                                >  1:1060944/1‑143 (MQ=255)
             aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGt                                                                                                                              >  1:1012328/1‑143 (MQ=255)
             aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGt                                                                                                                              >  1:982137/1‑143 (MQ=255)
                gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTcc                                                                                                                           >  1:1181560/1‑143 (MQ=255)
                       gCGTTTCATGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTaaaa                                                                                                                   <  2:314569/143‑1 (MQ=255)
                          tttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACCACTCCCGTCGACGGACAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCAGGCCCAAAAAGCCGGCGCTCGTGATATCTGCGGTCCGCTCAACAGt                                                                                                                >  2:460321/1‑143 (MQ=255)
                          tgtccggcggcTCACGGGCGGCATTGACTTCACCCTTAAACTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGt                                                                                                                <  2:180386/138‑1 (MQ=38)
                                   gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACGCCCGTCGAAGAAAAACCAGAGTACCCCCTCGTCCAGGAAGAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt                                                                                                       <  2:1014848/143‑1 (MQ=37)
                                     tCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa                                                                                                     >  2:595369/1‑143 (MQ=255)
                                     tCACTGGCGGCATGGACCTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTTTCCCAGGAATAATCCCGGGCCCAAATAGCCGGCGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa                                                                                                     <  1:82561/143‑1 (MQ=37)
                                     tCACTAGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAAACCCTGGCCCAAAAAGCCGGGGATCTTTATATCTGCGTGTCGCGAAAAAGTGCAGAGGAtga                                                                                                     >  2:1039352/1‑141 (MQ=255)
                                      cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg                                                                                                    <  1:1098230/143‑1 (MQ=255)
                                       aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg                                                                                                   <  1:1140489/143‑1 (MQ=255)
                                         tGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCGTCTTCGAGGACTAATCACTGGCCCAAAAAGCCGGGGATTATTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCACgtt                                                                                                 >  2:501363/1‑143 (MQ=37)
                                         tGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTAAATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt                                                                                                 >  2:377392/1‑143 (MQ=255)
                                                aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag                                                                                          >  1:1185136/1‑143 (MQ=255)
                                                aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcag                                                                                          <  1:934988/143‑1 (MQ=255)
                                                       tCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAACCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTg                                                                                   <  2:960084/143‑1 (MQ=255)
                                                          cccTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGt                                                                                <  2:982137/143‑1 (MQ=255)
                                                           ccTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTg                                                                               >  2:189171/1‑143 (MQ=255)
                                                            cTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAGAAAGTGCAATCGATCGGGTTGTTGCAGCATTTTACGTgg                                                                              >  1:226560/1‑142 (MQ=37)
                                                            cTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGACGGGGATCTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGATGTAGCAGCATCTTGCGTGa                                                                              >  2:1076068/1‑143 (MQ=37)
                                                                   tCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTGTAGATCTGCGTTCCGCTACAAAATCACAACTGTTTTTTGGTGGCAGCATTTTGCGTGACGGcgcg                                                                       >  1:632803/1‑143 (MQ=19)
                                                                     aacaacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGCCGGCTCggg                                                                     >  1:1809/1‑143 (MQ=255)
                                                                        agcTCCCGTCGAAGAAACGGCAGAGTAACGCCGCTTCCAGGAATAGTCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGGGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGaa                                                                  <  2:624379/141‑1 (MQ=21)
                                                                         acgcccGTCGCAGCCATATCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCACAAAAAGCCGGGAATTTGTACATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCGCGGGGAAg                                                                 <  2:251597/140‑1 (MQ=255)
                                                                         acTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg                                                                 <  2:858411/143‑1 (MQ=255)
                                                                           tCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCACGGGAGCAGGTTGTTGCAGGATTTTGCGTGGCGGCTCGGGGGAGGc                                                               >  2:701926/1‑143 (MQ=25)
                                                                           tCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGACGAGGATTTTTATATATGCGTTCCGCTAAAAAGTGCAAATGATCAGGATGTTGCAGCATTTAGGGTGAAGGCTCGGGGAAgga                                                               >  2:1119582/1‑142 (MQ=255)
                                                                             ccGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAACAAGCCGGGGATTGTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaa                                                             >  1:148286/1‑143 (MQ=255)
                                                                             ccGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAACGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaa                                                             >  1:87392/1‑143 (MQ=255)
                                                                               gTCGAAGAAAAAGCAGAGTAAATCCTCTTCCAGGAATAATCCCTGGCCTAAAAAGCCGGAGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa                                                           <  1:13624/143‑1 (MQ=255)
                                                                               ctgtaagatAAAGCAGAGTACATCCTCTTCTAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAGGAACAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa                                                           <  2:527526/139‑1 (MQ=18)
                                                                                         aaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga                                                 <  2:565108/143‑1 (MQ=255)
                                                                                         aaGCAGAGTAAACCCTCTTCCAGGAATAAGCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCaga                                                 <  1:634014/143‑1 (MQ=255)
                                                                                                gTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGACGGCAAAACTGCATCAGCGAAAGCa                                          >  2:5216/1‑143 (MQ=255)
                                                                                                     cccTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAAAATTATGACTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCg                                     >  1:237637/1‑143 (MQ=21)
                                                                                                       ctctTCCAGGAATAAGCCCTGGCCCAAAAGGTCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTg                                   <  2:566066/143‑1 (MQ=255)
                                                                                                        gctatcAGAAATCATCGCTGGCCCAAAACGCCGGGGAGTTTGATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTTCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgg                                  <  2:586650/138‑1 (MQ=18)
                                                                                                         ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTAAATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTggg                                 <  1:377392/143‑1 (MQ=255)
                                                                                                          ttCCAGGAATAATCCCTGGCACAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg                                <  1:616802/143‑1 (MQ=255)
                                                                                                              aGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTg                            >  1:822093/1‑143 (MQ=255)
                                                                                                              aGGAATAATCCCTGGCCCAAAAAGCCGGAGATTATTATATCTGCGTTCCGCTAAAAAGTGCAAATGACACGGTTGTTGCAGCATCTCGCGAGCAGCCTCGGGGAAGGCCAAACTGCACAAAAGAAACCACTGCGCGAGGACTg                            >  2:520510/1‑143 (MQ=12)
                                                                                                               ggAATAATCCCTGGCCCAAAAAGCCGGGGATGTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTgg                           <  2:1012328/143‑1 (MQ=255)
                                                                                                                gAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATGTTGCGTGACGCCTCGGGGAAGGGAAAAATAAATGGGGTAAAGCATTGCGTGGGGTCTGgc                          >  1:780155/1‑143 (MQ=17)
                                                                                                                 aataatCCCTGGCCCAAGAAGCCGGGTATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg                         <  1:197640/143‑1 (MQ=255)
                                                                                                                 aataatCCCTGGCCCAAAAGGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg                         <  2:730239/143‑1 (MQ=255)
                                                                                                                 aagactCCCTGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg                         <  1:992957/138‑1 (MQ=255)
                                                                                                                          tGGCCCAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCCCATcaac                >  1:611645/1‑143 (MQ=255)
                                                                                                                              ccAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCGTTGCGTGGGGTCTGGCGCCGGTCCACAATc            >  2:330294/1‑143 (MQ=17)
                                                                                                                              ccAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATCTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATc            >  2:448531/1‑143 (MQ=255)
                                                                                                                               cAAAAAGCCGGGGATTTTTTTATCTTCGTTCCTCCGAAAAGCCCGCGTGTTGGGATTGTTGGGGCATGTTGGGGGAGGGCTCGGGGGAGGCAGAACTGTAAGAGAGAAAGCATTACGTGGGGGCTGGGGCACGTCAAAAATCg           >  2:818708/1‑143 (MQ=255)
                                                                                                                               cAAAAAGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGCTCAGGTTGTTGCAGCATTTTGCGTGACGGCACGGGGAAGGCAAAACTGCATGACAGTAATCATTGCGGGGGGTCTGGCGCTCATCAACAATCg           >  2:257142/1‑143 (MQ=17)
                                                                                                                                aaaaaGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCgg          <  1:189171/143‑1 (MQ=255)
                                                                                                                                aaaaaGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTCGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAGTCgg          >  1:839293/1‑143 (MQ=255)
                                                                                                                                aaaaaGCCGGGGATTTTTATATCGGCGTTCCGCTAAAAAGTGCAAAGGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCgg          <  2:290482/143‑1 (MQ=255)
                                                                                                                                  aaaGCCGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCGAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTGATCAGCAGCCgggg        >  1:3182/1‑143 (MQ=18)
                                                                                                                                   aaGCCGGAGAATTTTATGTCTGCGTTCCGCTAAAAAGTGCAAACGCTCAGGTTGGTGCAGCATGTTGCGTGACGGCTCGGGGAAGCCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGa       <  1:1106773/143‑1 (MQ=18)
                                                                                                                                      ccGGGGATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTc    >  2:223942/1‑143 (MQ=255)
                                                                                                                                        ggggATTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGGcag  >  1:987779/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAG  >  NC_000913/3194426‑3194705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: