Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 742,267 T→C 13.9% E97G (GAG→GGG)  dtpD ← dipeptide and tripeptide permease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913742,2670TC13.9% 149.9 / 3.4 66E97G (GAG→GGG) dtpDdipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base T (38/18);  new base C (2/8);  total (40/26)
Fisher's exact test for biased strand distribution p-value = 1.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCC  >  NC_000913/742037‑742497
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         
ccAGGCCAACGCCCAGCGCCCAGCTGTACTCTCCCTGGGCGAACCCACAGGCGATAGGTGCAATAATAGCCCCCACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCa                                                                                                                                                                                                                                   <  1:660268/239‑1 (MQ=255)
      cAAAGCCCAGCGCCCAGCTGTCCTCTCCCGGGGCGTAACCACAGCAGAAAGGTGCAATAATAGCCCCCCCGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg                                                                                                                                                                                                                              <  1:576308/238‑1 (MQ=255)
      cAAAGCCCACCGCCCCGCTGTACTCTCCCTGGGCGTAACCACAGGGAAAGGGGGCGAAAATAGCCCCCACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg                                                                                                                                                                                                                              <  1:813816/238‑1 (MQ=255)
      aaaaGCCCACCGCCCGGCTGTACTCCTCCTGGGCATAACCGAAGGGGGTGGGGGCAATAATACCCCCCACTTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg                                                                                                                                                                                                                              <  1:163537/237‑1 (MQ=255)
                     aGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCCCCCGGGGCAGATAAAGGAATGACGGATGGATCTCAATGGCACCCAGcaccaacacaccgaa                                                                                                                                                                                                               >  2:794040/1‑228 (MQ=255)
                          tACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCAt                                                                                                                                                                                                        >  1:775241/1‑239 (MQ=255)
                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATcaccaa                                                                                                                                                                                                 >  1:761587/1‑238 (MQ=255)
                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGACACCCCGAACCACAAGACCGATGGACATCaaaaac                                                                                                                                                                                                >  2:363065/1‑239 (MQ=255)
                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAAAGGAATGACGGCTGGATCCCACTGGACCCCCACACCACATAACCGACGGCCACcaaaaaa                                                                                                                                                                                                >  2:276949/1‑238 (MQ=255)
                                       cGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATATAGGAATAACGGATGGATCTCACTGGCACCCATCACCAAATGACCGAACGCCATCAAAAACGccccc                                                                                                                                                                                          >  1:370558/1‑239 (MQ=255)
                                         tAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGCCAGCTTACGGTAGATTTAAACAGGCCATAGCCGCAGACGACAACCGCCAGGGACAGATACAGGAATGCCGGATGGATTCCCATGGCACCCAGCACCACATGACGGATCGCCATCAAAAACCCCCCCac                                                                                                                                                                                        >  2:194195/1‑238 (MQ=255)
                                           aCCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGGTCTCACTAGCACCCAACCCCACATAACCGCACACAATCAACAACGcaccccaca                                                                                                                                                                                      >  2:81162/1‑235 (MQ=255)
                                                       aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCATGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGCCGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACAAGACCGATCGCCATCAAACACGCCCCCCGCCTCACCCCCacgg                                                                                                                                                                          >  2:629107/1‑236 (MQ=255)
                                                       aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATGGCCAGGGACAGATAGCGGAATGACGGATGGATCTCACTGGGACCCACGCCACACTGACCGATCGCCACAAAAAAACCCCCAAACAACACCGcacagg                                                                                                                                                                          >  2:14389/1‑234 (MQ=255)
                                                       aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAAAAACGCCCCCAGCATCACCGCCATGc                                                                                                                                                                          >  1:705835/1‑239 (MQ=255)
                                                            cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAACGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACAACATAACGCATCGCCACAACAAACGCCCACAGCACCACCCCCAAGcagagc                                                                                                                                                                     >  2:436068/1‑234 (MQ=255)
                                                                   aGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGCATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACACGGCCCCCAGCATCACCGCCATGCGATTGACgcga                                                                                                                                                               >  1:286747/1‑238 (MQ=255)
                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGGTGGATCTCACTTGCCCCCAGCACCCCAGGCCCGATCGCCATCACAAAACCCCCCCGCATCACCGCCATGCGAAGGCCGAGAACATTa                                                                                                                                                         >  2:454902/1‑238 (MQ=255)
                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCCCCACATGACCGATCGCCATCAACAACGCCCCCAGCATCCACCCCATGCGAATCCCGGAGACTTTac                                                                                                                                                        >  2:284214/1‑238 (MQ=255)
                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCACCACCGCCATGCGATTGCCGAGACCTTTa                                                                                                                                                         >  1:205548/1‑238 (MQ=255)
                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCCCCCTCAACAACGCCCCCACCATCACCCCCAGGCGGTTGCCGGAAACttaa                                                                                                                                                         >  2:487199/1‑236 (MQ=255)
                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACAACATGACCGATCGCCATCAACAACGCCCCCAGAATCACCGCCATGCGATTGCCGAGAACTTTa                                                                                                                                                         >  1:204520/1‑238 (MQ=255)
                                                                         cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCCCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACCGCTAGAGGAATGCCGGATGGATCTCACTTGCACCCAGCACCACATGACCGATCGCCAGCAACAACCCCCCCAGCATCACCGCCACGCGCTTCCCGAGAACtttc                                                                                                                                                         >  2:670098/1‑237 (MQ=255)
                                                                           cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCCGCATCACCGCCATGCGATTGCCGAGAACTTTATc                                                                                                                                                       >  1:304475/1‑238 (MQ=255)
                                                                                   gccgcATACATCAGCGAGAAACCGCCATCACGAAGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTAACCGCCaaaaa                                                                                                                                              >  1:725864/1‑239 (MQ=255)
                                                                                     cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGCCGATAATCGCCAGGGACAGATAGAGGAAGGACGGATGGATCTCCATGGCACCCATCACCACATGACCGAACGCCCTCAACAACGCCCCCAGCCTCCCCGCCATACGACTGACGAGAACTTTACCCCCCaaaaaa                                                                                                                                             >  2:853565/1‑238 (MQ=255)
                                                                                     cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCCCATGACCGAACGCCCTCAACAACGCCCCCAGCATCACCCCACTCCCATTACCCAGCACTTTATCCACCAAAAAg                                                                                                                                             >  2:110148/1‑238 (MQ=255)
                                                                                         tACATCGGCGAAAAACCGCCATCACGGCGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCAcc                                                                                                                                        <  1:487199/239‑1 (MQ=255)
                                                                                               agggagaACCCGCCATCACGACGCGGATCGGTTGGCTAATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGAt                                                                                                                                  <  1:194195/236‑1 (MQ=255)
                                                                                                 cGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATCCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTg                                                                                                                                >  1:16805/1‑239 (MQ=255)
                                                                                                  gaaaaaCCGCCATCCCGACGGGGATCGGTTGGCTAATCAAGCTCGCCGAGCAGACAGTTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGa                                                                                                                                   <  1:794040/232‑1 (MQ=255)
                                                                                                   agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGACCTTTATCGGCCAAAAAACCACAGAGGATTGGc                                                                                                                              >  1:829573/1‑239 (MQ=255)
                                                                                                    aaaaCCGCCAACACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg                                                                                                                             <  1:276949/238‑1 (MQ=255)
                                                                                                     aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCCACAACGCCCCCAGCATCCCCGCCATGCGATTGCCGGGCACTTTATCCGCCAAAAAGCAACCGAAGAAGGGCgg                                                                                                                            >  2:54242/1‑238 (MQ=255)
                                                                                                      aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATCCGATTTCCGGGAACTTTATCCGCCAAAAAAACACCCAGGATTGGccgg                                                                                                                           >  2:98849/1‑236 (MQ=255)
                                                                                                              tCACGACGGGGATCGGTTGGCTCATACAGCTCCCCGAGCAGACAGCTTACGTTAGATTTAACCAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATacac                                                                                                                   <  1:584125/239‑1 (MQ=255)
                                                                                                                   acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGGGAAATTTATCCGCCAAAAAGCCACCGGGGATTGGGGTGAAATAAACCAGcc                                                                                                              >  2:798646/1‑238 (MQ=255)
                                                                                                                    cggggATCGGTTGGCTCATCAAGCTCGCCGGGCAGACAGCTTCCGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCg                                                                                                              <  1:284214/235‑1 (MQ=255)
                                                                                                                                           cccccccGAGCAAAAAGTTGATGTAAGATTAAAACAGCCCAGTGCCCGAAAGAAGAACCCCCAGGGACAGAAAGAGGAAGGACGGATTGACCTCACTGGCACCAAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                                                                       <  2:705835/234‑1 (MQ=255)
                                                                                                                                             tCGCCGGCCAGACAGCTTAGGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAGCGCCAGGGACAGATAGAGGAATGACGGATGGATCCCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg                                                                                       <  1:555177/237‑1 (MQ=255)
                                                                                                                                                      aGACATCTTACGTTAGATTTAAACAGGCCATGGCCGGAGACGATAACCGCCAGGGAAAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg                                                                             <  1:588767/238‑1 (MQ=255)
                                                                                                                                                       aacatttttcGTTAGTTTTAAACGGGCCAAGGCCCCACACGATAAACGCCAGGGAACGATAGAGAAATGACGGATGGATCCACCTGGCACCCAGCACCACATGACCGACCGCCATAAACACCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa                                                                           <  2:16805/230‑1 (MQ=255)
                                                                                                                                                         agtcTTGCGTTGGATTTAAACAGGCCATGCCGGCAGACTATAATCGACAGGGGCAGATGGGGGAAGGACGGATGGATCTCCCTGGCACCCAGCACCACATGCCCGATCGCCATCAACACCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTAt                                                                          <  2:370558/235‑1 (MQ=255)
                                                                                                                                                         aacgttACTTTAAATTTAAACAGGCCATGGCCGCAAACAATAATCGCCAGGGACAGATAGGGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa                                                                           <  1:794599/233‑1 (MQ=255)
                                                                                                                                                             ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACGGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg                                                                        <  1:480949/236‑1 (MQ=255)
                                                                                                                                                             atACGTTAGGTTTAAACAGCCAGTAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGCCGGATGGATCTCACTGGCCCCCAGCACCAAATGACCGATCGCCTTCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg                                                                        <  1:697760/235‑1 (MQ=255)
                                                                                                                                                                   taaaTTTAAACGGCCCATAGCCACAGGCGATAATCCCCAGGAACAGAAAGAGGAAGGAAGGGTGGATCTCACTGGCACCCACCCCCACAGGACCGATGGCAACAAACAACGCCCCAAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTAttt                                                                <  2:254710/235‑1 (MQ=255)
                                                                                                                                                                    tgttttAAACAGGCCCTTGCCCCAAACGTTAACCGCCGGGGAAAGAGAGAGGAATGCCGGATGCATCCCACTGGCCCCCGGCACCCAATGACCGATCGCCATCAACACCGCCCCCAGCATCCCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTa                                                              <  2:761587/236‑1 (MQ=255)
                                                                                                                                                                          aacagggCCATAGCCCAAGACGAAAACCCCCAGGGCCAGAAAGGGGAATGACGGATGGATCCCCCTGGAACCCACACCCAAATGCCCGACCCCCATCAACAACCCCCCCAGCATCCCCGCCATGCGATGGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt                                                        <  2:204520/234‑1 (MQ=255)
                                                                                                                                                                            acgggCCATAGCCGAAGGCGATAAGCGCCAGGGACAGATAGGGGAATGACGGATGGATCTCACTGGCACCCAGCCCCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg                                                      <  1:310674/236‑1 (MQ=255)
                                                                                                                                                                              aGGCCATAGCCGCAGACGATAACCGCCAGGGACAGATAGAGGAATGACGGATGGATCCCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCAAGCATCACCGCCATGCGATTGCCGGGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt                                                    <  1:10221/239‑1 (MQ=255)
                                                                                                                                                                               ggCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAg                                                             >  1:881711/1‑229 (MQ=255)
                                                                                                                                                                                     aGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACCCCAGCGAACAGTAGGCGCAAAATAACACGTAGGCGTGGGTATCGGTGTATTTTAGTTGATTGgagagaaaa                                            >  2:310674/1‑236 (MQ=255)
                                                                                                                                                                                          cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCCCCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTAATTGGGGAGAAAAAGaaa                                       >  2:432441/1‑238 (MQ=255)
                                                                                                                                                                                          cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCCCCGAGGATTGCGGGGACATACACCAGCGAAACGTAGCCGCTAAATAACTAGTAGGCGGGAGAATCGTTTTATTTTATTGGGTTGGGAAAAAAGAGaaa                                       >  2:854028/1‑238 (MQ=255)
                                                                                                                                                                                          cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGCAGGCGTGAGAATCGTTGTATTTTAGTTGATTGGTGGGATAGAGAAt                                       >  1:123638/1‑239 (MQ=255)
                                                                                                                                                                                              agaaaaTCCCCAGGGCAAGATAGAGGAATGCCGGATGGATCCCACTGGCCCCCAGCACCAAATGACCGATCGCCATCAACAACGCCCCAAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcag                                    <  1:432441/234‑1 (MQ=255)
                                                                                                                                                                                                  aagcGCCAGGGACAGATAGGGGAATGGCGGATGGATCCCAGTGGCACCCAGCCCCCCATGACCGATCGCCTACAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAggg                               <  1:110148/236‑1 (MQ=255)
                                                                                                                                                                                                         aGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAAGCGCCAAATAACACGAAGGGGGGGGTACCGTTGTATTTTAGTTTATTGGTGAGCAAGAAAATACAAAGGGCACgaa                         >  2:311728/1‑236 (MQ=255)
                                                                                                                                                                                                           ggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGGGAGATAGAGAATCAGCAGGGCACGCATg                       >  1:79884/1‑238 (MQ=255)
                                                                                                                                                                                                               aattgggggAATGAAGGTTGGACCCCATGGGCACCCAGCACCACTTGCACGATCGCCATCAACAACGCCCACAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa                  <  1:336101/232‑1 (MQ=255)
                                                                                                                                                                                                                     agGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGACCAGTATGCGCTAAATAACTCACAGTAGTGAGTATCGTTGTATTTTAGATGAGTGGAGAGATAGAGAATCAGCCGGGCCCGCATACCGTAAAAGca            >  2:123165/1‑238 (MQ=255)
                                                                                                                                                                                                                     agGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCt            >  1:262410/1‑239 (MQ=255)
                                                                                                                                                                                                                       gAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGCCGTGACATACACCAGCGAACAGTACGCGCTAAATACCTCGTAGGCGCGAGTACCGTTGCATTTTAGTTGATTGGTGAGATAGAGAATCACCAGGGCACGCATGCCGTaaaacttaa          >  2:158948/1‑234 (MQ=255)
                                                                                                                                                                                                                             cGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAaaat     >  1:96285/1‑238 (MQ=255)
                                                                                                                                                                                                                               gatggatCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATAAACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTTGATTTTAGTTGTTTGGGAGAATAGGGAATCAGCAGGGCCCCCATGCCGAAAAAATAAAAAAATTcc  >  2:67699/1‑239 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         
CCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACATGACCGATCGCCATCAACAACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCC  >  NC_000913/742037‑742497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: