Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,561,294 T→C 13.5% intergenic (+396/‑74) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,561,2940TC13.5% 153.4 / 2.3 65intergenic (+396/‑74)yffL/yffMCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (33/22);  new base C (4/5);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTGTACGACTGCAGCGAAG  >  NC_000913/2561058‑2561524
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      
aTTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGGCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCa                                                                                                                                                                                                                                      <  1:853788/239‑1 (MQ=255)
 ttAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCCCAACCTGAACACCAGACCAACAAACCATCATCGCATTCGTCCATGGGGAGCGGGGAATAACTCaa                                                                                                                                                                                                                                     >  2:177481/1‑239 (MQ=255)
 ttAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGAAAATCACaa                                                                                                                                                                                                                                     >  2:365751/1‑239 (MQ=255)
      aaTTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGGAACGCCCATTCACTAACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGAGGAGCGCGGGATATCTCAAAtggg                                                                                                                                                                                                                                >  1:816993/1‑238 (MQ=255)
         tCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTAAAAgggcact                                                                                                                                                                                                                             >  1:641906/1‑235 (MQ=255)
             ggCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATGGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCt                                                                                                                                                                                                                         <  1:345796/239‑1 (MQ=255)
                  cGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGACCGCGGAAAATCTCAAAAGGAAATTTCTTATtg                                                                                                                                                                                                                    >  2:18123/1‑239 (MQ=255)
                  cGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTAACGAACAACCTGACAAGCAGACCAACAAACCATCATGGAATTCGTTCATGGGGCGCGCGGTAAATCTCAAAGGGGGATTGCTTATtg                                                                                                                                                                                                                    >  1:156531/1‑239 (MQ=255)
                     gAGTTTTTTTGCCCCCCCGGCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAAGTATTTCGCCGCCTGATAGGGCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATtggt                                                                                                                                                                                                                  <  2:8529/238‑1 (MQ=255)
                     gAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCGAGAGTTAAACAGAAGATTTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTACCGCACCACCGGACCAGCAGACCAACAAACCATCATGGCTTTCGTTCATGGGGATCGCGGGATATCTCCAAGGGTGTTTTTTTCTtggtg                                                                                                                                                                                                                 >  2:585608/1‑239 (MQ=255)
                                    ccGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCCCAACCTGAACCGCAGACCAACAACCCACCATGGCATTCGTTCATGGGGCACGCGGTATATCTCAAAGGGCGATTTTTTATTGGTGGGCCTTAGGAAgcat                                                                                                                                                                                                  >  1:281675/1‑236 (MQ=255)
                                          ggtcATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGAATATCCCAAAGGGGAATTTCTATTTGGTGGGCCTTCGGAAgagtttgggc                                                                                                                                                                                            >  2:364598/1‑230 (MQ=255)
                                                cgtgagGCGCCTAAAGTTAAACAGAAGAATTATTTCGCCCCCTCATAGGGCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTGCCAGCAAGCTGGAAACGCCCATGCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGc                                                                                                                                                                                      <  2:501414/236‑1 (MQ=255)
                                                agagagATGCCTAGATGTAAACGTAAGAATTATTTCGGCACCTGATTGGTCGACTGGTTGCGAGCATTAACCATGCTGGTTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGc                                                                                                                                                                                      <  1:380704/239‑1 (MQ=255)
                                                   gggTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCa                                                                                                                                                                                    <  1:882607/236‑1 (MQ=255)
                                                      tCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCCTGGGAGGCCCGGTATATCCCAAAgg                                                                                                                                                                                                                                  >  2:518743/1‑189 (MQ=255)
                                                      tCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGGGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCtt                                                                                                                                                                                >  2:395161/1‑239 (MQ=255)
                                                      tCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGAGGGCCTTCGGAAGGCTTCGGGCAATTGCCGGCtt                                                                                                                                                                                >  1:203126/1‑239 (MQ=255)
                                                      tCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCCCAAAGGGGGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTTCCAGCta                                                                                                                                                                                >  2:267892/1‑238 (MQ=255)
                                                      tCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACCCACAACCTGAAAAGCAGACCAACAAACCATCATGTAATTCGTTCATGGGGAGCGCGGGATATCTCAAAGGGTGATTTCTTATTTGTGGGCCTTCGAAAGGCTTCGAGCAATTGCCAGCta                                                                                                                                                                                >  2:270747/1‑238 (MQ=255)
                                                             gatttAAATAAAAGATTTAATTCGCACCCTGATAGGTCGGCTGGTTTCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGTAAACGCACATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCAAGCTTAAAAGAc                                                                                                                                                                         <  2:354882/236‑1 (MQ=255)
                                                             gagTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGAc                                                                                                                                                                         <  1:18123/239‑1 (MQ=255)
                                                               gTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGAc                                                                                                                                                                         <  1:267892/237‑1 (MQ=255)
                                                                ttAAACAAAAAAATTTTTTCCCCGCCTGATAGGTCGGCTGGTTGCGGACATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACggg                                                                                                                                                                      <  2:846875/239‑1 (MQ=255)
                                                                  aaaCAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCt                                                                                                                                                                    <  1:716256/239‑1 (MQ=255)
                                                                  aaaCAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCt                                                                                                                                                                    >  1:355521/1‑239 (MQ=255)
                                                                        aagaaTTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAg                                                                                                                                                              >  1:562240/1‑239 (MQ=255)
                                                                         agaaTTTATTCGCCGGCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGt                                                                                                                                                             <  1:365751/239‑1 (MQ=255)
                                                                             ttattTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTGCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATg                                                                                                                                                         >  1:594404/1‑239 (MQ=255)
                                                                                                  cGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCCCAAAGGGGGATTTCTTATTGGTGGGCCTTCGGAAGTGTTCGAGAAATTGCCAGCTTTAAGAACGGGCTTAGCAGTGATGGTTATCCGCCACGAATTTCCg                                                                                                                                    >  2:394905/1‑239 (MQ=255)
                                                                                                   ggCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGTGGGGc                                                                                                                                                                                                              >  2:427445/1‑164 (MQ=255)
                                                                                                   ggCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGc                                                                                                                                                                                                              <  1:427445/164‑1 (MQ=255)
                                                                                                       ggtttggAGCATTAACCAGGCTGGTAAGCCAAATCCTTTCCAGCAAGTCGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCata                                                                                                                               <  1:454898/233‑1 (MQ=255)
                                                                                                       ggTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGAGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTCGCAGGGATGGTTATCCGCCACGAAGGTCCGCCata                                                                                                                               >  1:387907/1‑239 (MQ=255)
                                                                                                            ggggcttcaaCCATGCTGTTTAGCCAAATCCTTTCCACCAAGCTGGAAACGCCCATTCCTCACCGCACAACCTGAACAGTAGTCAAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATattgca                                                                                                                          <  2:275725/231‑1 (MQ=255)
                                                                                                                          gCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCAAGCACAACCTGCACAGCAGACCAACAAACCATCATGGCATTCGTTCCTGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCTTTCGAAAGGCTTCGAGCAATTACCAGATTAAAAGACGGGCTTAGCAGAGGTGGTGATCCGGCACAACTGTCCGCAATATTGAATTGCGCAAAAAcgg                                                                                                            >  2:842804/1‑238 (MQ=255)
                                                                                                                            tGATTAGCCCAATTATTACCAGCAAGGTGGAAACGCCCATTCACTCACGCACAACCTGACCAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAAcgcg                                                                                                           <  2:208145/238‑1 (MQ=255)
                                                                                                                                        tCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGCATGTCCGTCCAATTTCATTGGGCAAAAACGCGGGGGACTGAGGGa                                                                                              >  2:151392/1‑239 (MQ=255)
                                                                                                                                        tCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTTCGCGGAAACGCGTGGCACTGAgggg                                                                                              >  1:454953/1‑238 (MQ=255)
                                                                                                                                        tCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCAGGCAATTGCCAGCTTAAAAGACGGGCTTGGCAGTGATGTGTATACCCCACGATAGTCCGCAATATGGCTTTACGCAGAAACGGGGGGCACGAggggg                                                                                             >  2:251805/1‑237 (MQ=255)
                                                                                                                                                aGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCCTATTGCATTGCGCAGAAACGCGTGGCACTGAGGGGGTTTTCCg                                                                                      >  1:333827/1‑239 (MQ=255)
                                                                                                                                                  cAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGaa                                                                                                                >  1:236696/1‑211 (MQ=255)
                                                                                                                                                             cccccTTTCCCACACGCACACCTCGAACGCAAACTCAAAAAAACACAATGGCATCCGTTCAGGGGGAGCGCGGTATACCTCAATGGTGGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGTTGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTggg                                                                         <  2:777002/237‑1 (MQ=255)
                                                                                                                                                                        ccacGCACAACCTGAACAGCAGCCCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCCCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTccc                                                              <  1:177481/238‑1 (MQ=255)
                                                                                                                                                                                   ccTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATCTTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTggg                                                    >  2:751815/1‑238 (MQ=255)
                                                                                                                                                                                       aaCAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGAGCCTTCGGAAGGCTTCGAGCAATTGACAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGTGCAGAAACGCGTGGCCCTGAGGGAGTTTTCCTAATTAGTTTTGGGGACCCTTTCCCTGTTTTTGGAGaaa                                                >  2:695442/1‑239 (MQ=255)
                                                                                                                                                                                           gaagACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGGATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGGTTTCCGAATTAGTTTTGGGGAACCTTTCCCTGCTTCTGGAAAAAAAGa                                            >  2:675772/3‑239 (MQ=255)
                                                                                                                                                                                            cagACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGAc                                           >  1:542177/1‑239 (MQ=255)
                                                                                                                                                                                             agACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACg                                          >  1:306058/1‑239 (MQ=255)
                                                                                                                                                                                              gACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCCCTGGGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGGGCAAAAGAcaa                                         >  2:163396/1‑237 (MQ=255)
                                                                                                                                                                                                ccAAAAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGa                                         <  1:175761/237‑1 (MQ=255)
                                                                                                                                                                                                  caacaaACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGGGCAGAAACGCTTGGCCATGAGGGAGCTTTTGGAATTGGTTTTGGGAGCCCTTTCCCTGCTTCTGGGAGAAAAgacggacg                                      >  2:380840/1‑239 (MQ=255)
                                                                                                                                                                                                  caacaaACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACg                                      >  1:116045/1‑239 (MQ=255)
                                                                                                                                                                                                       aaCCTTCTTGGCATTGTTTTATGGGGGGGCGGGTAATTTTAAAAGGGTGATTTTTTATTGGGGGGCTTGGGGAAGGCTTCGGCCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCaaa                                 <  2:562240/239‑1 (MQ=255)
                                                                                                                                                                                                         ccctcatGGCTTTCTTGCAGGGGGGGCGCGTTATATCTCAAAGGGTGATTTCTTATTGGGGGCCCTTCGGAAGGCTTTGAGCATTGGCCTGCTTAAAAGATGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATg                               <  2:454953/236‑1 (MQ=255)
                                                                                                                                                                                                            tcatGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGGTATCCGCCACGAATGTCCGGCAGATTGCATTGCGCAAACACGAGTGGCACTGAGGAGGTTTTCGGATTTAGTTTTGGGGACACTTTCCCTGCTTCTGGAACAAAACACCAAGAGCAAAGGCaa                            >  2:144698/1‑238 (MQ=255)
                                                                                                                                                                                                              ctGGCTTTCGTTTCTGGGGGGCGGGGTATATCTCAAAGGGTTATTTTTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTg                          <  2:542177/238‑1 (MQ=255)
                                                                                                                                                                                                                    ttCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGACGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGGGATGGTTATCCGCCACGAATGTCCGTATAATTGCTGTGCGCCGAACAGCGTGGCACTGCGGGAATTTTCCGACTTAGTTTTGGGGACCCTTTCCCTGATTCTGGGGCAAACAACGAAAGGAAAATGCGAGGCTTCCt                    >  2:169164/1‑239 (MQ=255)
                                                                                                                                                                                                                    ttCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGCATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCt                    >  1:897341/1‑239 (MQ=255)
                                                                                                                                                                                                                      tgTTCAGGGGCGGCGTGGTATATCCCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAACTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTAGCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTg                  <  1:167349/238‑1 (MQ=255)
                                                                                                                                                                                                                          cATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGGGCAAAAGCGGAACGGAAACTGCAGTGGTTCCTTGTAc               >  1:726439/1‑238 (MQ=255)
                                                                                                                                                                                                                                     cgGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTGTACGACTGCAGCga    <  1:585608/238‑1 (MQ=255)
                                                                                                                                                                                                                                     cgGTATATCCCAAAGGTGGAGTTCTTATTGGTGGGCCTTGGGAAGTCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTGTACGACTGCAGCga    <  1:828821/238‑1 (MQ=255)
                                                                                                                                                                                                                                     cgGTATATCCCAAAGGGGTATTTCTTATTGGGGGGCCTTCTGAAGGCTTCCAGAAATTGCCAGGTTAAAAGACGGGCTTAGCAGTGATGGTTAGCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTGTACGACTGCAGCga    <  1:865172/238‑1 (MQ=255)
                                                                                                                                                                                                                                       gTATATCCTAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCGTCGGACAATTGCCAGCTTAAAAGCCGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTGTACGACTGCAGCgaag  <  1:343329/238‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      
ATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCCGCCTGATAGGTCGGCTGGTTGCGAGCATTAACCATGCTGATTAGCGCAATCATTTCCAGCAAGCTGGAAACGCCCATTCACTCACGCACAACCTGAACAGCAGACCAACAAACCATCATGGCATTCGTTCATGGGGAGCGCGGTATATCTCAAAGGGTGATTTCTTATTGGTGGGCCTTCGGAAGGCTTCGAGCAATTGCCAGCTTAAAAGACGGGCTTAGCAGTGATGGTTATCCGCCACGAATGTCCGTCATATTGCATTGCGCAGAAACGCGTGGCACTGAGGGAGTTTTCCGAATTAGTTTTGGGGACCCTTTCCCTGCTTCTGGAGCAAAAGACGAACGGCAAATGCAGTGCTTCCTTGTACGACTGCAGCGAAG  >  NC_000913/2561058‑2561524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: