Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,739,684 T→C 18.3% E129G (GAG→GGG)  tyrA ← fused chorismate mutase T/prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,739,6840TC18.3% 129.6 / 4.6 61E129G (GAG→GGG) tyrAfused chorismate mutase T/prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (29/20);  new base C (3/9);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 5.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCAGAGCTTCCGCCTCTGCACGACGCGAGGCCAACA  >  NC_000913/2739449‑2739912
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    
cAGGCTACCCGTGGCCGGCCCGAACATCGGGGGTAGCCCCAGCTCCGGGCCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCGTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCa                                                                                                                                                                                                                                   <  1:198657/239‑1 (MQ=255)
          cttgccGGACCCAACATCGGGTGTGGCCCCAGCCCCGGACCATCATGCCCCCCCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCAc                                                                                                                                                                                                                         <  2:485678/235‑1 (MQ=255)
                   ccGAACAGCGGGTGTAGCGCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAAc                                                                                                                                                                                                                 <  1:200004/238‑1 (MQ=255)
                       aCATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGc                                                                                                                                                                                                                           <  1:601600/224‑1 (MQ=255)
                       aCATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCgag                                                                                                                                                                                                            <  1:508696/239‑1 (MQ=255)
                       aCAGCGGGTGTAGCCCAAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCgag                                                                                                                                                                                                            <  1:442897/239‑1 (MQ=255)
                        aagcggtggtAGCCCCCGCCCCGGACACTCAGGCGCCCCCAGCATGGCCTGTAATGGCCCATTTTCCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCCCCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGGCAGGTTGCTCCAGAATCCGCACCTGATAACCCgaga                                                                                                                                                                                                           <  2:259015/231‑1 (MQ=255)
                                 tacccccACCCCCGGCCCCTCATGCCCCACCAGCTTGGCCTGTAATGGCCAATTTTTCCTTGATGCCAGATCGCCCAGAATACAATCTTTCGTGAAAGGCGGTAATTTGCCAATACCTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCa                                                                                                                                                                                                  <  2:88127/236‑1 (MQ=255)
                                 tAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCAACCTAACAATCACCATTCCGGCACCGGCAACAACATCCGCCGCTCGACCCCAGTCATTTTTCTCCCTAATCCCCACCTCTAAACCCCAGACGGGCacc                                                                                                                                                                                                   >  2:508696/1‑236 (MQ=255)
                                        aGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCCCCAGAATCCGCACCTGATAACCCGAAAGGGGCAGCAtctttcc                                                                                                                                                                                           >  1:728925/1‑236 (MQ=255)
                                         gCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGTTCAGTAACGGGGATTGGCCAACTAACAAACAACATTCCGGCATCGGCAAACAAATAAGCCCCCCGATCCCCACAACTTTGTTCCAAAACCCACACCCCACAACCCCGGAGGGTCAACATCTTCTCg                                                                                                                                                                                          >  2:804965/1‑239 (MQ=255)
                                             cGGACCATCATGCGCCCCCAGCGTGGCCTGGAATGTCCCATTTTTCACTGATGCCAGAACGACCAGAATACAAACTTTGTGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACa                                                                                                                                                                                      <  2:108774/239‑1 (MQ=255)
                                               gACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTCACAATCACCATTCCGGCATCGGCAACAAATTCAGCCGCTCGATCCCAGCCATGTTGCTCCCGAATCCGCACCTGGTAACCCGCGAGGGCCACCAcctttccggaaagg                                                                                                                                                                                    >  1:86471/1‑229 (MQ=255)
                                               gACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCACCTTCTCGAACAg                                                                                                                                                                                     >  1:150073/1‑238 (MQ=255)
                                                    tcatGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCCTCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCACCCCCTCATAACCCCAGAGGGTAAGCAACTTCTCGAACAAGCGTcc                                                                                                                                                                               >  2:721743/1‑239 (MQ=255)
                                                    tcatGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAAAATCAACCGCCCCATCCCAGCCCTGTTTCTCCACAAACCGCCCCCGATAACCCGAGAGGGTCCACATCTTCTCaaaaaaggacc                                                                                                                                                                                >  2:149401/1‑230 (MQ=255)
                                                         cgcCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTTGCACACTCACAATCACCATTCCGGCATCGGCAACAATATTAGCCGCTCGATCCCAGTCATGTTGCCCCCGAATCCCCACCTGATAACCCGCGAGGGTCAGCATCTTCTCGACCAGGCGTCCCa                                                                                                                                                                             >  1:701346/1‑236 (MQ=255)
                                                            caccaGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGt                                                                                                                                                                                                       <  1:537111/207‑1 (MQ=255)
                                                                      gCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAAATTGCTCAGAAACGTGGATTGGCCCACTAACAAACACAATTCCGGCATTGGCAACAATATAAGACGCCCGATCCCAGTCATGTTGTTCCCAAATCCGAACCTGGTAAACCGTGAGGGCAAGCATCTTCTACAACAGTCGGCCCCCCTGaacgccaccgcc                                                                                                                                                             >  1:66613/1‑239 (MQ=255)
                                                                            aagggCCCATTTTTCACGGAGGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCCCCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGAt                                                                                                                                                       <  1:477131/236‑1 (MQ=255)
                                                                            aaTGGCCCATTTTTCACTGATGCCAGATCGCCCAAAATAAAATCTTGCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGAAACGTGGATTGGCACACTAACAATCCCCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGAt                                                                                                                                                       <  1:94817/239‑1 (MQ=255)
                                                                            aaTGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCCCCCGAATCCCCACCTGTTAACCCGACAAGGTCCGCAACTTTTCGAACAGGCGTCCCACCCTAcccccaccccccacagc                                                                                                                                                       >  2:734188/1‑236 (MQ=255)
                                                                                 cccATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTTCGGCATCGGCAAACATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGGCAACCACTTCTCGAAAAGGCGGCCCATCCGACCCCCCCCCCCGACGATaaac                                                                                                                                                   >  2:751510/1‑236 (MQ=255)
                                                                                 cccATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGACGTCCCACCTGACCGCCCCCGCCACCGAGAacc                                                                                                                                                   >  1:310678/1‑238 (MQ=255)
                                                                                        ttCACTGATCCCAGACCGACAAGAATCCAATCTTTCGGTAAGGCGGTTATTTTGCCAATAACTTGGTCAGTACCTGGGATTGGCACACTAACAATCCCCTTCCCGGACTCGGCAAAAAGACCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACg                                                                                                                                           <  2:701346/239‑1 (MQ=255)
                                                                                              tgtGCCAAATCCACCAAAATAACATTTTTCGGTAAAGGGGGTAATTTGCAAAAAACTTGCTAAGTAACTTGGTTTGGCACCATAACAATCCCCATCCCGGCACCGGCAACAATACCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGa                                                                                                                                     <  2:150073/237‑1 (MQ=255)
                                                                                              gctGCCAAATCGCCCAAAAAAAAATCTTTGGGAAAGGGCGTATATTTCCAAATACTTGGCCCATAAACGGGGTTGGCCCCCCAACCAACCCCCTTCCCGGCATCGGAAAAAACATCAGCCCCTCGACCCCGGTCATGTTGCCCCAAAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTCCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGa                                                                                                                                     <  2:804204/237‑1 (MQ=255)
                                                                                                     gATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAAAAGGCGTCCCATCTGCCCGCCACCGCCGACGATAACACACGGACGCAGTGACGGACaa                                                                                                                              >  1:328114/1‑239 (MQ=255)
                                                                                                        ctcacaaaaTACATTCTTTGTGAAAAGGGCGTAATTTTCCAAATACGTGCCTAATAACGGGGTTTGGCACTCTAACACACCCCATCCCGGCCTCGACAACAATCCCCGCCGGCCGATCCCATTCATGTTTCTCCAGAACCCGCCCCGAATAACCGGGGAGGGTCAGCATCTTCTCGAACAGGCTTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt                                                                                                                           <  2:681195/232‑1 (MQ=255)
                                                                                                        cGACCAGAATCCAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCCCACTAACAATCCCCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt                                                                                                                           <  1:688886/239‑1 (MQ=255)
                                                                                                                         ttCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTTATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGGGACGGACAAAAGGTTTTAAATCCTTTTTc                                                                                                          >  1:718527/1‑239 (MQ=255)
                                                                                                                         ttCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGCGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGGGACGGACAAAGGGTTTTAAATCCtttt                                                                                                            >  1:547122/1‑236 (MQ=255)
                                                                                                                         ttCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGGTTTAAATCCTTTGTc                                                                                                          >  1:19056/1‑239 (MQ=255)
                                                                                                                         ttCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGAAGCAGTGCCGGACAAAGGGTTTTAAATCCTTTGTc                                                                                                          >  1:576237/1‑239 (MQ=255)
                                                                                                                         ttCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCCCCGACGATAACCACCGGACGCCGTGACGGCCCAAGGGTTTTAAATCCtttt                                                                                                            >  1:540385/1‑236 (MQ=255)
                                                                                                                         ttCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGGCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACCGTAACCACCGGACCCAGGGACGGCCAAAGGGTTTTTAATCCTTTTTc                                                                                                          >  1:395450/1‑239 (MQ=255)
                                                                                                                                  aggagttattttGCCAATACCTTGCCCAGTAACTTGGTTTGGCACACTAACAATCCCCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTCCTCCAGAATCCGCCCCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCAc                                                                                                  <  1:819057/230‑1 (MQ=255)
                                                                                                                                         aaTTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATTCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGGCCCATCTGACCGCCCCCGCCTGCGAAAACCACCGGACGCAGGGCCGGACAAAGGGTTTTAAAACCTTTGTCGTTTTTCCTGGAGTa                                                                                           >  2:880572/1‑239 (MQ=255)
                                                                                                                                                             aGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGGGTTTTAAATCCTTTGTCGGTTTCACTGGAGTAAAATTCACCCATAAcccac                                                                        >  2:772335/1‑236 (MQ=255)
                                                                                                                                                               tAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGc                                                                     >  1:871097/1‑239 (MQ=255)
                                                                                                                                                                aaagTGGATTGGCCCCCAAACAATCCCCATTCCGGCATCGGCAACAATACCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGAc                                                                       <  1:149401/233‑1 (MQ=255)
                                                                                                                                                                aaCGTGGATTGGCACACTAACAATCACCATCCCGGAATCGGCAACAATATCAGCCGCTCGATCCCAGTCATTTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGCCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGAc                                                                       <  1:721743/236‑1 (MQ=255)
                                                                                                                                                                   gtgttttGGCCCCCAAACACTCCCCATCCCGACATCGCCAACAATCACTGCCCCCCGACCCCGGTCATGTGGCCCAAAAACCCCCCCCTGATACCCCGAGAGGGTCAGCATCTACTCGAACAGGCGTCCCATCTGACCGCCTCCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCaaaa                                                                 <  2:196698/234‑1 (MQ=255)
                                                                                                                                                                    tGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCCGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTAACTGGAGGAAGATTCAAGAATAACCCGACGAAAAAc                                                                >  2:297212/1‑239 (MQ=255)
                                                                                                                                                                            cccacTCACCATCCCCCTTCCGGCCTCGGCAAAAATTCCAGCCGCCCGCTCCCAGTAATGTTGCCCAAGAACCCGCACCTGATACCCCGAGAGGGTCAGCATCTTCCCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCaa                                                        <  2:395450/237‑1 (MQ=255)
                                                                                                                                                                             ccccTAACAATCCCCTTTCCGCCTGCGGCAAAAATACCCGCCGCGCGATCCCAGTCATGTTGCCCCAGAATCCCCCCCTGATACCCCGAGAGGGTCATCATCTTCTCGAACAGGCGCCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAAt                                                       <  2:728925/236‑1 (MQ=255)
                                                                                                                                                                                cTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGCCGTTAACCACCGGACGCAGGGACGGACAAAGTGTTTTAAATCCCTTGTCGTTTTCACTGGAGTAAAATTCACGCACCACCCGCCGCAAAAAATCCCCaaaaag                                                    >  2:351270/1‑235 (MQ=255)
                                                                                                                                                                                   aCAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACCAAGCGGTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCCCGCATCAACCCACCCAAAACAACCCCAAtaaaaca                                                 >  2:155886/1‑233 (MQ=255)
                                                                                                                                                                                      aaTCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGGGTTTTAAATCCTTTGTCGTTTTCCCTGGAGTAAGATTAACGCCTAACCCGACGCAAAACATCCTCAAACaacacgg                                               >  2:619202/1‑233 (MQ=255)
                                                                                                                                                                                          aCCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGACTCACCCCACGCAAAACATCCTCAATCAGACCTGGCgg                                           >  2:135259/1‑239 (MQ=255)
                                                                                                                                                                                            ctttCCGGCACGGGCAACAATATCAGCCGCTCGATCCCATTCAGGTTGCCCCAGAACCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTa                                         <  1:351270/237‑1 (MQ=255)
                                                                                                                                                                                            cATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGCCAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGCGTAAGATTCACGCATCACCCGACGCAAAAAAACCTCAATCAGaccggagggaa                                         >  2:337782/1‑229 (MQ=255)
                                                                                                                                                                                            cATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGCCGCCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGGGACGGACAAAGGGTTTTAAATCCTTTGTCGTTTTCACAGGAGTAAGATTCAAGACACACACCACCCAAAACAACCCCATCCAGATCGGGCggaa                                         >  2:717487/1‑237 (MQ=255)
                                                                                                                                                                                            cATTCCGGCAACGGCAACAATACCCGCCGCCCGATCCCAAGCATGTTGCCCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTa                                         <  1:804965/239‑1 (MQ=255)
                                                                                                                                                                                              ttCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGGGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGCCCCCAAACATCCCCAATCAAATCTGGCGGGac                                        >  2:772023/1‑238 (MQ=255)
                                                                                                                                                                                                  ggCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCCCCTTAGAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCa                                   <  1:832329/239‑1 (MQ=255)
                                                                                                                                                                                                    cctgggCAAAATTATCAGCCGCTCGATCCCAGTCATGTTTCCCCAAAATCCGCCCCTGATACCCCGAGGGGGTCCGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCaga                                 <  2:208359/235‑1 (MQ=255)
                                                                                                                                                                                                                          tCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGCCGGACAAAGGGTTTTAAATCCTTTGTCGTTTTCACTGGAGTACGATTCAGCCATCACCCGACGCAAACCATTCCCAATCAACTCTGGCGGTAGACACAAAGCTTCCCCCTCTGCacaaccag           >  2:385263/1‑236 (MQ=255)
                                                                                                                                                                                                                            gaacccAGGCATGGTGCCCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCCCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCAGAGCTTCCGCCTCTGCACGACGCGAg         <  1:535444/236‑1 (MQ=255)
                                                                                                                                                                                                                                cctatCATGTTGCCCCAGAACCGCCCCCTGATAACCCGGAGGGGTCAGCATCTTCTCGACCAGGCGTCCCATCGGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCAGAGCTTCCGCCTCTGCACGACGCGAGGcta     <  1:332655/235‑3 (MQ=255)
                                                                                                                                                                                                                                   gTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCAGAGCTTCCGCCCCCTCCCGACCCCAGGCCacaa  >  1:294585/1‑236 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    
CAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTAATGGCCCATTTTTCACTGATGCCAGATCGACCAGAATACAATCTTTCGGTAAAGGCGGTAATTTGCCAATAACTTGCTCAGTAACGTGGATTGGCACACTAACAATCACCATTCCGGCATCGGCAACAATATCAGCCGCTCGATCCCAGTCATGTTGCTCCAGAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGCATCACCCGACGCAAAACATCCTCAATCAGATCTGGCGGTACACCCAGAGCTTCCGCCTCTGCACGACGCGAGGCCAACA  >  NC_000913/2739449‑2739912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: