Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,744,004 T→C 14.2% L127P (CTC→CCC)  yfiB → OM lipoprotein putative positive effector of YfiN activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,744,0040TC14.2% 176.1 / 3.9 75L127P (CTC→CCC) yfiBOM lipoprotein putative positive effector of YfiN activity
Reads supporting (aligned to +/- strand):  ref base T (36/27);  new base C (2/10);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCTTATCAGATGCCATTCTGTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTAC  >  NC_000913/2743768‑2744240
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
tgggttttCAGATGCCATTTTTTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAACCCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTca                                                                                                                                                                                                                                             <  2:289917/232‑1 (MQ=255)
     tATCAGATGCCATTCTGTTCGCAAAAAATGACTACAAATTGCCCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACcaca                                                                                                                                                                                                                                       <  1:215987/239‑1 (MQ=255)
     tATCAGATGCCATTCTGTTCGAAAAAAATGCCTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACcaca                                                                                                                                                                                                                                       <  1:508895/239‑1 (MQ=255)
      tagagagtacATTCTGGTCGCAAAAAATGACTACAAATTGCCCCCGGAAAGCCAGGAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACcaca                                                                                                                                                                                                                                       <  1:359090/229‑1 (MQ=255)
      aTCAGATGCCATTCTGTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGTCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTccccaccc                                                                                                                                                                                                                                      >  1:665648/1‑237 (MQ=255)
             gCCATTCTGTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCACCGCAGCAATCTCCCCACACAGGGttt                                                                                                                                                                                                                               >  1:733862/1‑239 (MQ=255)
                aTTCTGTTCGCAAAAAATGCCTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGTTTGACACACCGACAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCACGGGCTATGGGTGGACAAATTCAACGCAGCAATCTCACAACACAGGGTTTTgg                                                                                                                                                                                                                            >  1:723466/1‑239 (MQ=255)
                aTTCTGTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCCCACCACACAGGGTTTAgg                                                                                                                                                                                                                            >  1:261890/1‑239 (MQ=255)
                 ttCTGTTCGCAAAAAATGACTACAAATTGCTCCCAGAAAGCCAGGAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGa                                                                                                                                                                                                                           <  1:795036/239‑1 (MQ=255)
                    tGTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaa                                                                                                                                                                                                                        <  1:887030/239‑1 (MQ=255)
                      ttCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACCGCGCAATCTCACCACACAGGGGAAAGGaaaaaa                                                                                                                                                                                                                      >  2:752713/1‑239 (MQ=255)
                        tgCAAAGAATGGCTACCAATTGCTCCCGGAAAGGCAGCAACAGATGCAAACCAGGGCGGATTAATTGTCCACGACAGGGTTAACATATGCCCGTAAGGATTGACAAAACGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAat                                                                                                                                                                                                                    <  2:254929/238‑1 (MQ=255)
                             aaaaTGACTTCAAATTGCTCCCGGAATGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACAGGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATccc                                                                                                                                                                                                               <  1:29925/239‑1 (MQ=255)
                                 tGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACCCATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAg                                                                                                                                                                                                           <  1:262953/239‑1 (MQ=255)
                                    cTACAAATTGCCCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATGGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGcc                                                                                                                                                                                                         <  1:188739/238‑1 (MQ=255)
                                        aaaTGGCCCCCGGAAACCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGCCAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTaa                                                                                                                                                                                                    <  1:703361/239‑1 (MQ=255)
                                             gcccccGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGGGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGa                                                                                                                                                                                               <  2:722725/236‑1 (MQ=255)
                                             gCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACCCATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGa                                                                                                                                                                                               <  1:397892/239‑1 (MQ=255)
                                                             cAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGAAACAAGACCGCCCAGGGCcgcg                                                                                                                                                                                >  1:179741/1‑238 (MQ=255)
                                                                   aTCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCCTGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACCCAGGGTTTAGGAAAAAAACATCCCATAGCCACTAACAAGACCCCCCAAGGCCGCGCCGaaaa                                                                                                                                                                         >  2:743117/1‑239 (MQ=255)
                                                                    tCCAAACCATGGAAGATAAATGTGCTCCGGTAGGGTTAACACATGCCCGTTGGGATGGACACACCGATAACTATGGGGAAGACAGTTACAACGAAGGCTTTACATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGAAAAATTCCACGCAGCAATCTCACCCCACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAc                                                                                                                                                                        <  2:395311/239‑1 (MQ=255)
                                                                        aaCCATGGCAGCTAAATTGGCCTCGACAGGGCTAACAAATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGACGAATGTCGTGGCCGATGCCTGGGCTAAGAGCGGAAAAATTCCACGCAGCAATTCCACAACACCGGGTTTAGGGAAAAAATATCCATTAGCCAGTCAAAAGACCGCCCAGGACCCCCCCGAGaaaagc                                                                                                                                                                     >  2:215324/1‑234 (MQ=255)
                                                                         aCCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgccg                                                                                                                                                                   <  1:149614/239‑1 (MQ=255)
                                                                            aTGGCATCTAAATTGGCCTCGGAAGGGCTAACACATGCCCGTATGGATGGACACCCCGATAACTAGGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGt                                                                                                                                                                <  1:167646/239‑1 (MQ=255)
                                                                             tGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGCAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGGGGACAAATTCCACGCAGCAATCTCACCCCACAGGGTTTAGAAAAAAAATATCCCCAAACAAGTAACAAAACACCCCCAGGCCACGCCAAGAAACACCCCGtg                                                                                                                                                               >  2:484040/1‑238 (MQ=255)
                                                                             tGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACCCAGGGTTTAGGAAAAAAATATCCCCTAACCAGCAACAAGACCGCCCAGGGCCCAGCCGaaaaactccccgc                                                                                                                                                                >  2:181649/1‑229 (MQ=255)
                                                                              ggCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGACGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGAATTGGCTATGGGTGGACAAATTCCACGCATCAATCTCACCACCCAGGTGTTAGGAAAAAAATATCCCAAAGCCATTACAAACACCACCCAGGGCCGGCCCGAAACCCGCcgcgcg                                                                                                                                                               >  2:598963/1‑236 (MQ=255)
                                                                               gCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACcaca                                                                                                                                                                                                                                       <  1:197234/165‑1 (MQ=255)
                                                                               gCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACcaca                                                                                                                                                                                                                                       >  2:197234/1‑165 (MQ=255)
                                                                                 agttAATTTGGCCCCGAAGGGGTAAACCATAGCCCGTATGGATGGCCAACCAGATAATTGGGGTGAAGACAGTAAAAACGAGGTCTTATCATTGAAACGGGCGAATGTCGTGGCCGAGGCATGGTCTATGGGTGGAAAAATTCCACGCAGCAATCCCACCACACAGGGTTTAGAAAAAAAATACCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCa                                                                                                                                                            <  2:261890/235‑1 (MQ=255)
                                                                                  gCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGCCAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAAACCGCCCAGGGCCGCGCCCAGAACCGCCGCGCCCCAgg                                                                                                                                                          >  2:137231/1‑238 (MQ=255)
                                                                                   gttatttGGCCTGGAAAGGGCTAACACATCCCCGTTGGGATGGACACCCCAATAACTATGGTGAAGCCAGTTACAACGAAGGTTTAGCATTGAAACGGGCGAATGTCGTGGCCGATGAATGGGCTTGGGGTGGAAAAATCCCCCGCAGCAATCTCCCCACCCAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgtg                                                                                                                                                         <  2:210287/234‑1 (MQ=255)
                                                                                   gtAAATTGCACTCGCTAGGGCTACCCACTGTCCGTATGGATTGACACCCCGATAAATATGGGGAACGCCTTTAACACGGAGGCTTAGAATGGAAACGGGCGAATGTAGGGGCCGATGCACGGGCTAGGGGTGGAAAAATCCCACGCAGCAATCTCCCCACACAGGGTTTAGGAAAAAAATACCCCATAGCCAGTAACAAGACCGCCCAGTGCCGCGCCGAGAACCGCCGCGTCGCAgtg                                                                                                                                                         <  2:303712/238‑1 (MQ=255)
                                                                                    tAAATTGGCCTTAACCGGGCTAACCCAGCCCTGTTGGGATGGACCACCCAATACATATGGTGAAGCGAGTTAAAGCGAAGGATATCCATAGAACCGGGCGAATGTCGGGGCCGATGAATGGCTAAGGGGTGGACAAATCCCACGCACCAACATCCCCCCACGGGGTTAAGAAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCCGGGCAGCGACCAGAACCGCCGCGTCGCAgtg                                                                                                                                                         <  2:723466/238‑1 (MQ=255)
                                                                                    tAAATGGGCCAGGAAGGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGGAAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgtg                                                                                                                                                         <  1:752713/238‑1 (MQ=255)
                                                                                                     ggCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAAACCGCCCAAGGCCGCGCCGAGAACCGCCGCGTCCCAGTGGTGCTTCCCCACCCTTaa                                                                                                                                       >  1:803255/1‑239 (MQ=255)
                                                                                                               tGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGGGGGGATTACTACCCCTTCAAACACCAGCa                                                                                                                             >  1:266777/1‑239 (MQ=255)
                                                                                                               tGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAAACCGCCCAGGGCCGCGCCGAGAACCCCCGCGTCTCAGGGGTAATTCCACCCCCTAAAAACACCacaa                                                                                                                             >  2:310681/1‑236 (MQ=255)
                                                                                                                 gCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATAGGCTATGGGTGGACAAATTCCCCGCACCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATTGCCACTAACAAGACCGCCCAAGGCCCCGCCCAGAACCCCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACAccccca                                                                                                                             >  1:479797/1‑234 (MQ=255)
                                                                                                                  cccGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCCCGCCGCAGTGGGGATTACTACCCCTTAAAACACCACCaaa                                                                                                                           >  1:708350/1‑239 (MQ=255)
                                                                                                                    cGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTaaaa                                                                                                                                     >  1:32890/1‑227 (MQ=255)
                                                                                                                      tATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAAACCGCCCAGGTCCGCGCCCAGAACCCCCCCGCCCCAGGGGGGATTCATACCCCTTAAAAAACCAACAAAAAGa                                                                                                                       >  2:611212/1‑239 (MQ=255)
                                                                                                                      tATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAAACCGCCCAGGGCCGCCCCGGAAACCCCCGCGTCCCAGTGGGGATTACAACCCCTTAAAAAACCAGAAAAAAGa                                                                                                                       >  2:29299/1‑239 (MQ=255)
                                                                                                                           atggaCACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGGAATTACTACCCCTTAAAAAACCAGCAACCAGATaaaa                                                                                                                  >  1:101252/1‑239 (MQ=255)
                                                                                                                                   acCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGCCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGgcc                                                                                                           >  1:276499/1‑238 (MQ=255)
                                                                                                                                   acCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAAAAAAACCGCCCAGGGCCGCGCCGAGAACCCCCGCGTCGCAGTGGAGATTACTACCCCTTAAAACACCAGCAAACAGAAAAAAAAGGgcca                                                                                                          >  2:717021/1‑239 (MQ=255)
                                                                                                                                              tGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGGGGACAAATTCCCCGCAGCACTCTCCCCCCACAGGGTTTAGGAAAAAAAAATCCCATAGCCAGTAACAAGACCGCCCAGGGCAGCGCCGCGAACCGCCGCGTCGCACCGGGTATTACTACCCCTTCAAACCCCCACAAACAACAAAAAAAGCGCCACCCAATTAGcc                                                                                               >  2:567301/1‑239 (MQ=255)
                                                                                                                                                   aaGACAGTTACAACGAAGGTTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGAAGCAATCTCCCCACACAGGGTTTAGGAAAAAAATACCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGcc                                                                                           <  1:712443/238‑1 (MQ=255)
                                                                                                                                                      aCAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATACAAAAGGGCCAGCCAATTGGCCAGCCct                                                                                         >  1:160401/1‑237 (MQ=255)
                                                                                                                                                          ttACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTaa                                                                                   >  1:738698/1‑239 (MQ=255)
                                                                                                                                                          ttACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAACCCTTCTTaa                                                                                   >  1:4247/1‑239 (MQ=255)
                                                                                                                                                          ttACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAAAACCAGCAAACAGAAAAAAAAAGGCCACCCAATTGGCCACCCCTCCTaaa                                                                                   >  2:334851/1‑239 (MQ=255)
                                                                                                                                                                       tttgctttaaaaCGGGGAATGGTGGTGGCCGTTGCATGGGGTATGGGTGAAAAAATCCCCCGCACCAATCCAACCAAACGGGTTTAAGGAAAAAAAAACCCCAAAGCCAGTAAAAAGACCGCCAGGGCCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCt                                                                       <  2:266777/229‑1 (MQ=255)
                                                                                                                                                                               gAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCACCCCTTCTTAAAAGGAGTTCCCTTAAGCGaaa                                                              >  2:634984/1‑239 (MQ=255)
                                                                                                                                                                                     gggaaaTGTCGGGGCAGATGCGGGGGCGTGGGGTGAAAAAATCCCCCGCAGCAAGCCCACCACAAGGGTTTTAGGAAAAAAAACCCCCATAGCCAGTAACAAGCCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATc                                                            <  2:665648/231‑1 (MQ=255)
                                                                                                                                                                                      gCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGGGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAAAGAAATCtttat                                                       >  2:64889/1‑236 (MQ=255)
                                                                                                                                                                                           tGTCGTGGCCGATGCATGGGTTTTGGGTGGCAAAATTCCCCGCAACAATCTCCCCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGa                                                  <  1:467650/239‑1 (MQ=255)
                                                                                                                                                                                           tGTCGGGGCCGATGGTGGGGCTTGGGGTGAACAAATCCCACGCAGCAATCTCCACCAACAGGGTTGAAGAAAAAAATAGCCCATAGCCAGTAACAAGACCGCCCAGGGCGGAGCCGAGAACCGCCGCGTCGCAGTGGTTAGTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGTCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGa                                                  <  2:598866/239‑1 (MQ=255)
                                                                                                                                                                                              gctgtcccGATGCCGGGGTGAAGGGTGGACAAAGCCCCCGAAGAAATCCCCCACCACGGGGTTTAGTAAAAAAATCTCCCATAGCCAGTAACAAGACCGCCCGGGTCCGAGCCGAAAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACCCCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACg                                                <  2:60597/233‑1 (MQ=255)
                                                                                                                                                                                               cggtgCCTATGCATGGGTTAAGGGTGGACAAATTCCACGAAGCAATCCCACCACCCGGGGTTTAGGAAAAAAATACCCCATAGCCAGTAACAAGCCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACg                                                <  1:64889/234‑1 (MQ=255)
                                                                                                                                                                                                 tGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGGTAAGACGc                                               >  1:364175/1‑237 (MQ=255)
                                                                                                                                                                                                            tGGGCTAGGGGGTGACAAATTCCACGCAGCAACCCCACCACACAGGGTTTAGGAAAAAAACATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGa                                   <  1:743117/238‑1 (MQ=255)
                                                                                                                                                                                                             gggCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACa                                                                                                                                                                                                  <  2:316009/78‑1 (MQ=255)
                                                                                                                                                                                                             gggCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACa                                                                                                                                                                                                  >  1:316009/1‑78 (MQ=255)
                                                                                                                                                                                                                  aTGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCCCTTTGATACGAGCAGCCt                            >  1:169700/1‑239 (MQ=255)
                                                                                                                                                                                                                  aTGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAAACGCCCTTTTAAACGAACACCCt                            >  2:674529/1‑239 (MQ=255)
                                                                                                                                                                                                                  aTGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTAACCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCt                            >  1:815815/1‑239 (MQ=255)
                                                                                                                                                                                                                  aTGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGGGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTaa                                                                                   >  1:378705/1‑184 (MQ=255)
                                                                                                                                                                                                                         gACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGt                     >  1:87702/1‑239 (MQ=255)
                                                                                                                                                                                                                                 cccccgagcaACCTCACCACAGAGGTTTAAGTAAAAAACAATCCAATGGCCAGAAACAAGCCCGCCAAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCAc             <  1:567301/233‑1 (MQ=255)
                                                                                                                                                                                                                                  gcccccagcaTCTCACCCACACGGGGTTGAGGAAAAAATACACACATCGCCAGTAACAAGCCCCCCCGGGGCCGCGCCGAAGACCGCCGCGTCGCAGCGGTGATTCCTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCAc             <  2:202518/230‑1 (MQ=255)
                                                                                                                                                                                                                                    cccagcaATCCCACCACACAGGGTTAAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAAAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCa          <  1:196827/237‑1 (MQ=255)
                                                                                                                                                                                                                                     gcaccaACCCCACCACACAGTGTTTAGGAAAAAATATCCCAGACCCAGTAACAAGACCCCCCAGGGCCGAGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAgg        <  1:823629/239‑1 (MQ=255)
                                                                                                                                                                                                                                         aaaTCCCACCACACAGGTTTTAGGAAAAAAATCCCCCATAGCCAGTAACAAGCCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgtag     <  1:334851/238‑1 (MQ=255)
                                                                                                                                                                                                                                           aTCCCACCACACAGGGTTTAGGAAAAAAATACCCCAGAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgtagta   <  1:634984/239‑1 (MQ=255)
                                                                                                                                                                                                                                            tctcACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGGAGTAc  >  1:49640/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
GGGCTTATCAGATGCCATTCTGTTCGCAAAAAATGACTACAAATTGCTCCCGGAAAGCCAGCAACAGATCCAAACCATGGCAGCTAAATTGGCCTCGACAGGGCTAACACATGCCCGTATGGATGGACACACCGATAACTATGGTGAAGACAGTTACAACGAAGGCTTATCATTGAAACGGGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTAC  >  NC_000913/2743768‑2744240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: