Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,796,104 C→T 15.0% S144F (TCT→TTT)  csiR → transcriptional repressor of csiD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,796,1040CT15.0% 125.9 / 2.3 56S144F (TCT→TTT) csiRtranscriptional repressor of csiD
Reads supporting (aligned to +/- strand):  ref base C (12/35);  new base T (6/3);  total (18/38)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTA  >  NC_000913/2795869‑2796339
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           
gTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTtctc                                                                                                                                                                                                                                           <  1:843051/238‑1 (MQ=255)
  ggCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGTTGAGTGGGATCTGCGTCCTCAGGCGTTTCATACGGCAATTGTGGCGGGGGTGGGGTtctc                                                                                                                                                                                                                                           >  1:833533/1‑237 (MQ=255)
   gCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTTTGGTTCTCCCtt                                                                                                                                                                                                                                       >  1:367980/1‑238 (MQ=255)
      tgtATGTCAGAGAAGGGGCTGCTCGATATTTTCGGCGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAtt                                                                                                                                                                                                                                     <  1:881913/236‑1 (MQ=255)
      tCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGGGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAtt                                                                                                                                                                                                                                     <  1:36492/238‑1 (MQ=255)
      tCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGAGGTGGCGTTGATTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGCGGAAGCTTGAGGCCTGTGACGCCAGCGAGAACAACCCTGATGGGAGGGATCTGCGTCATCCGGGGTTTCATACGGCAATTGGGGGGGGGCGTGGGtcctccttat                                                                                                                                                                                                                                     >  2:715604/1‑230 (MQ=255)
       cagtGTCAGGGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAtt                                                                                                                                                                                                                                     <  1:692336/234‑1 (MQ=255)
            tCAGAGCCGGGTCTGTGCTATATTTTCGACGCCCGCGCCAATATGGAAGGTGGGTGAGTGAGTCTGGCGATTGCCCGCGGGGGGGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCa                                                                                                                                                                                                                              <  2:731407/239‑1 (MQ=255)
             cAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGGCTTCAGGCGTTTCATACGGCAATTGTGGGGGGAGGCGGTGTTCACTATTtgcggaca                                                                                                                                                                                                                             >  2:209709/1‑234 (MQ=255)
                     gAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCGGTGGGTCTCCCACTTTTTTGCAAATGCGTGa                                                                                                                                                                                                                     >  1:638205/1‑239 (MQ=255)
                      aGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTtc                                                                                                                                                                                                                                             <  1:696103/214‑1 (MQ=255)
                       gttgtgtgttatTTTCGACGCCCGGGGCAATATGGAAGCGATGTTGGGGGGTTTGGGGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGaa                                                                                                                                                                                                                    <  2:51589/229‑1 (MQ=255)
                        ctgctCGATATGTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAAc                                                                                                                                                                                                                   <  1:670598/238‑1 (MQ=255)
                                  tttttcgcccccGGGCCAATTTGGAAGCGATGTTGGGGAGTCTGGGGGTTGGCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGAt                                                                                                                                                                                                        <  2:596838/230‑1 (MQ=255)
                                          gCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTAATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTggcgg                                                                                                                                                                                                 <  1:575645/238‑1 (MQ=255)
                                            ccGCGCCAATATTGATGCGATGTTGGTGGGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGGTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATATGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGgcggcg                                                                                                                                                                                               <  2:350589/238‑1 (MQ=255)
                                             cgctgcAATATGGAAGCGATGTTAGGGAGTCTGGCGGTTGCCCGGGGTGGCGATGAGTGGGAGGCAGACGTTCTCGAAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGcgcg                                                                                                                                                                                             <  1:70349/234‑1 (MQ=255)
                                                gcCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGaa                                                                                                                                                                                                                    <  1:719010/213‑1 (MQ=255)
                                                gacAATAGGGAAGCGATGTTAGTGAGTCTGGGGATTGCCCGCGGTGGCGAGGAGTGGGAGGCAGACGTTGTCGAAAGAGCGCATCTGCTGAGTAAGCTTGAGGTCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTa                                                                                                                                                                                          <  1:836046/237‑1 (MQ=255)
                                                         gAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTAt                                                                                                                                                                                 <  1:371303/239‑1 (MQ=255)
                                                          aaGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTTCCTATTTGCTGCAAATGCGGGAACGGTTGTTTGATCTGGCGGCGCGTTATCGGtttttt                                                                                                                                                                                >  1:683859/1‑236 (MQ=255)
                                                          aaGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGAGGGATCTGCGTCATCAGGCGTTTCATCCGGCAATTGTGGCGGGCTTGGGGTTTCACTATTTTGTGCAAATGCGTGAACGGGTGGTTGAGTTTGGGGCGGGTCATCGATTTAta                                                                                                                                                                                >  2:424963/1‑238 (MQ=255)
                                                            gCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGGTGTTTGGTCTGGCGGGGCGTTTTCGATTTTTCTg                                                                                                                                                                              >  1:480362/1‑239 (MQ=255)
                                                            gCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGGTGTTCTCCCTATTTTTTGCAAATGCGTGAACGGGTGTTTGTTTGTGCGGGGCGTTGTctgtttttttg                                                                                                                                                                              >  1:246809/1‑229 (MQ=255)
                                                               aTGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCCGTGGTTTTCCCCATTTGCTTCAAATGCGTGAACGGTGGTTTGATTTGGCGGGGGGTTTTCCATTTTTCTg                                                                                                                                                                              >  1:440233/1‑236 (MQ=255)
                                                                tGTTAGTGAGTCTGGCGAGTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTtgctgc                                                                                                                                                                                                                               <  1:28015/186‑1 (MQ=255)
                                                                     gggAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTgcggcg                                                                                                                                                                     <  1:798950/237‑1 (MQ=255)
                                                                          tCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTTTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTTGCGGGGCGTTATCGATTTATCTGGCTGCGGGGAACgg                                                                                                                                                                >  1:451782/1‑239 (MQ=255)
                                                                          tCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGGTCTCCCTCTTTTCTTCAAAAGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTTTCGATTTTTTTGGATTGAGCGAACgg                                                                                                                                                                >  1:306554/1‑239 (MQ=255)
                                                                          tCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGGGGTTTTCACAATTTGTTGCAAATGGGGGAACGGTTGGTTTATTTTGGGGGGCGTTTTCGGTTTTTTTGGGTTCGGGGAACgg                                                                                                                                                                >  1:846013/1‑239 (MQ=255)
                                                                          tCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGGGGGTTTCACTCTTTTCTGCAAATGCGGGCACGGTTGTTTGATCTGGGGGCGCGGTCTTTGTTTTTCTGGCTGGGGGGAACgg                                                                                                                                                                >  2:667619/1‑239 (MQ=255)
                                                                            tGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTTCTGCAAATGCGTGAACGGGTGTTTGATCATTCGTGCCGGTATGCATTTATCTGGCTGCGGCGAACGGTg                                                                                                                                                              >  1:111748/1‑239 (MQ=255)
                                                                            tGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGGTTTCACTATTTTCTGCAAATGCGTGAACGGGTGTTTGGTCTTGCGCCGCGTTATCCATTTATCTGGCTGCGGCGAACGGt                                                                                                                                                               >  1:747949/1‑238 (MQ=255)
                                                                               cggttGCCCGGGGGGGGGGTGGGGGGGGGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGGGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCt                                                                                                                                                            <  2:496943/235‑1 (MQ=255)
                                                                                        gcgGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTTTGGGTCTCACTATTTTCTGCAAATACGGGAACGGTTGTTTGATCTGGCGGGGCGTTTTCGATTTATCTTGCTTGGGCGAACGGTGCTTTTGGTGGaa                                                                                                                                                  >  1:496943/1‑239 (MQ=255)
                                                                                               tgttGAGTGGGGGGCCTACGTTCTGGCAAAAGCGAATTTGTTTAGTTAGGTTGAGGCCCGTGACGCCAGCAAGGAAATGCTTGATGAGTTGGATCTGCGTCATCAGGCGTTTCATACGGTAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGATTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                                                                                            <  2:725372/235‑1 (MQ=255)
                                                                                               cgaggggTGGGAGGGCGACGTACTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAGTGCTTGATGGGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                                                                                            <  2:106972/232‑1 (MQ=255)
                                                                                               cGATGGGGGGGAGGCAGACGGTCCCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                                                                                                            <  1:622905/238‑1 (MQ=255)
                                                                                                    aGTGGGAGGCAGACGTTCCGGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTggagga                                                                                                                                       <  1:174931/238‑1 (MQ=255)
                                                                                                      tGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTTCTGCAAATGCGGGAACGGTTGTTTGATCTGCCGGCGCGGTTTCGATTTTTTTGGCGTGGGGGAAAGGGGCTTTCGGTGGAAAAACTGGGGGATaa                                                                                                                                    >  1:35154/1‑239 (MQ=255)
                                                                                                         gAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAac                                                                                                                                  <  1:794303/238‑1 (MQ=255)
                                                                                                          aGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAaca                                                                                                                                 <  1:176944/238‑1 (MQ=255)
                                                                                                           ggCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAtt                                                                                                                                                                                                                                     <  1:506/137‑1 (MQ=255)
                                                                                                              aggcGTGCTCGAAAAAGCGTGTCTGCTGGGTAGGCTGGAGGCCTGGGACGCCAGCGAGAAAATGCTTGATGAGGGGGATCTGGGTCATGAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGGTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGAt                                                                                                                             <  2:370587/235‑1 (MQ=255)
                                                                                                              aGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCGTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGAt                                                                                                                             <  1:209709/238‑1 (MQ=255)
                                                                                                                   ttgtcGCAAAAGCGCCTTTGCTGGTTAAGCTTTGGGCCTTGGACGCCAGGGAGAAAATGCTTGATGAGGGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCAc                                                                                                                       <  2:389839/236‑1 (MQ=255)
                                                                                                                    tttccaaaaaGCGCATCTTGTGTGTAATGTTTGGGGCTGTGGGGCCAGTGAAAAAATGCTTGATGAGTGGGATCTGCGTCAACAGGCGTTTCATACGGCAATTGGTGCGGGCTGGGGTTCTCCCTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGCTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCAcc                                                                                                                      <  2:328999/233‑1 (MQ=255)
                                                                                                                                   tttgctgAGTAAGCTTGAGGCCTGTGACGGCAGCGAGAAAATGCTTGAGGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTgcgg                                                                                                       <  1:773281/237‑1 (MQ=255)
                                                                                                                                          aGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTTTCTGGCTGCGGCGAACGGGGGTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAAACCCCGACTTcggggggaat                                                                                                >  1:392925/1‑235 (MQ=255)
                                                                                                                                            taaggtttgggCCTGTGACGCCAGGGAGAAAATGCTTGATGGGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTg                                                                                               <  1:792419/229‑1 (MQ=255)
                                                                                                                                            tAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGGGCTTTCGGGGGAAATGCTGGAGGGTAAACACGATCAGCACCAGACCCTGCCTTCGCCGGTACttg                                                                                              >  1:602644/1‑237 (MQ=255)
                                                                                                                                             aaGCTTGAGGCCTGTGACGCCAGCGAGAAAATGGTTGAGGGGTGGGATCTGGGTCATCAGGCGTTTCAGACGGGAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTAGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGgc                                                                                             <  2:476848/239‑1 (MQ=255)
                                                                                                                                                      gtcTGTGCAGCCAGCGAAAAAATTCTTGGTGAGGGGGAGCTGCGTCAGTAGGCGTTTCATACGGGAATTGTGGCGGTCTTTTGTTCTCACTATTTGCTGCAAATGCGGTAACGGTTGTTTGATCTGGCGGCGCGTTAGCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATa                                                                                     <  2:833533/236‑1 (MQ=255)
                                                                                                                                                            ggccgctgCGAAAAAATGTTTGGGGGGGGGGTTCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTTTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACcgcgcg                                                                              <  2:807320/232‑1 (MQ=255)
                                                                                                                                                                               ttGATGAGTGGGCACGGCCTCATCAGGGCTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATTTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATgcgc                                                           <  1:485505/239‑1 (MQ=255)
                                                                                                                                                                                                   cttccggCGTTTCATTCGGCAATTGGGGGGGGTTTGGGTTCTCACTTTTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAAt                                       <  2:480362/234‑1 (MQ=255)
                                                                                                                                                                                                            tttCATTCGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGGGTGAACGGTTGTTTGAACTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTa                               <  1:574569/238‑1 (MQ=255)
                                                                                                                                                                                                                               cGGGCTGTGTTTTCCCCTATTTGCTGCAAATGCGTGAACGTTTGTGTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGAtggctgg            <  1:686617/238‑1 (MQ=255)
                                                                                                                                                                                                                                  ggCTGGGGTTTCCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTAGCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCa          <  1:206605/238‑1 (MQ=255)
                                                                                                                                                                                                                                         gTTCTCAGTATTTGCAGCAAAGGCGCGAACGGGTGTTTGATGTGGCGGGGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGGGAAAAGGCTGGAGGATAAACACGATCAGCCCCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAacta  <  1:136782/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           
GTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTA  >  NC_000913/2795869‑2796339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: