Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,479,689 G→A 15.8% L307F (CTC→TTC)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,479,6890GA15.8% 127.7 / 2.2 54L307F (CTC→TTC) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (31/14);  new base A (2/7);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 2.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCG  >  NC_000913/3479484‑3479924
                                                                                                                                                                                                             |                                                                                                                                                                                                                                           
cgttctacaaGCATCGGCTGGGTCATAATGTCAAGCGCTCCCGTCACCAGCACCACGCCCATTGGGTTCCCCTGAAATAAGTTTTTTGAAGACGCGGTAAAAAAGAGGACAAAGGCAACCTCACCACCCGGTCTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATTCAACAGCGCCACCAGTCACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTAt                                                                                                                                                                                                            <  2:558703/230‑1 (MQ=255)
       tcaGCAACGGGGTGGTCAACAGGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCGGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACCCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTAcc                                                                                                                                                                                                     <  1:768163/239‑1 (MQ=255)
           cAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAAAATTTTCACCGCCACCAGCCCAACCACCCTTATCACTACCcaca                                                                                                                                                                                                 >  1:149954/1‑239 (MQ=255)
              cGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTTACCTCCGCACGCCATCCAATCCCGCCAGCAGAACCAGCCCCAAAATTTTCACCCCCCCCACCCCAACCACCCCTAacacccacccaaaaa                                                                                                                                                                                              >  2:581869/1‑228 (MQ=255)
                 cttGGTCATAATGGAAAGCGTCACCGTCACCAGCAACAACGCCATGTGGTCGCCCTGGAATAAGCGTTTGGAAAAAGCGGTAGAAAAGAGGACAAAGGCAAACTCCCCACCCTGCCTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTCCGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGa                                                                                                                                                                                            <  1:47845/236‑1 (MQ=255)
                     gTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATACCTACCCccaaccaatgct                                                                                                                                                                                        >  1:421476/1‑231 (MQ=255)
                                aaGCGTCACCGTCACCAGCAACAACGCCATCTGGTCCCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCCCCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCAcc                                                                                                                                                                             <  1:206042/238‑1 (MQ=255)
                                   cgtcaccgtcacCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCAATCCAATCGCGCAAGCAAACACAGCACGAGAATTTCAACCGCCACCAGACCCACCCAGCTTATCACCACCAACAAAAAAAGGGCCAAAAACAcccccc                                                                                                                                                                         >  2:222948/1‑237 (MQ=255)
                                    gtcacggtcaccACAAACAACGACTTCGGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGAAAAAGGCAAACTCCCCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGATCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGa                                                                                                                                                                         <  1:563624/238‑1 (MQ=255)
                                       accgtcaccAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGCGCTACTCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCCCAACCCCGCTTATCACCAACCCCAAAAGATGGGAATAAAGCACCCAGaagat                                                                                                                                                                     >  2:155767/1‑235 (MQ=255)
                                       accgtcaccAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAAATGGGTATAAAGCACCCCGCGGtt                                                                                                                                                                     >  1:889343/1‑239 (MQ=255)
                                                  caacaaCGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGAATAAAGCACCCCGAGGTTGAGTGACATg                                                                                                                                                           >  1:214638/1‑238 (MQ=255)
                                                   aacaacGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGAATAAAGCACCCCGAGGTTGAGTGACATGc                                                                                                                                                          >  1:518981/1‑238 (MQ=255)
                                                     caacGCCAGCTGGTCCCCCTGGAAAAAGCGTTGTAAAGAAGCGGTAAAAAAGAGGACAAAGGCAAACTCACCACCCTGACCCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGa                                                                                                                                                       <  1:515008/239‑1 (MQ=255)
                                                        ccgcagtgggTCGCCCTGAAAAAAGGTTTGTAAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCCCCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACag                                                                                                                                                    <  1:196396/231‑1 (MQ=255)
                                                            aTCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCCCAACCACGCTTATCACTACCCACAACATATGGTCATAAAGCACCCCGAGGTTGAGTGCCATGCCGACagaaga                                                                                                                                                >  1:850535/1‑236 (MQ=255)
                                                             tCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTGTAAAGCACCCCGAGGTTGAGTGACAGTCCGACAGAGATa                                                                                                                                               >  1:736052/1‑239 (MQ=255)
                                                              tgggccccacTGGAATAACGTTTTTGAAGAGGCGTTAGAAAAGAGGACAAAGGAAAACCCCCCCCCCTTACTACACAGCCCAGAAAACTCCATCCGCTCTGAGCTCAGCACCCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATaa                                                                                                                                              <  2:436610/230‑1 (MQ=255)
                                                                  tCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACCGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAAAAGGGACAAAAGCAACCCGCGGTTGAGTGACATCGCGCCAGGAAAAAAGaa                                                                                                                                          >  2:887849/1‑239 (MQ=255)
                                                                   cGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGAACGAGGTAAAGAAc                                                                                                                                         >  1:734816/1‑239 (MQ=255)
                                                                                    tgtgAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGATGGGTGACATGCCGACCGAAATAAAGAACAAACCGAGCAACAAg                                                                                                                          >  2:807220/1‑237 (MQ=255)
                                                                                         agaagCGGAAAAAAAGAGACAAAACGCAACCCCACCCCCCTTCACCAAACCCCCAGCAAACTCCATCCGCTCGGACCCTCCGACCCCAGAAAATCGCCCCATCAGATACAGCACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACATAGATAAAGAACAAACCGAGCAGCAAGCCTTTg                                                                                                                    <  2:26104/238‑1 (MQ=255)
                                                                                           aagCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGAATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGCGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                 >  1:717181/1‑239 (MQ=255)
                                                                                            gggggaaGAAAAGAGGACAAAGGCAAACTCACCACCCTGCCCCAACACGCCAGCAAACTGCATCCGCACTGAGCTACGCACGCCATACAAGCGCGCAAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                 <  1:891294/232‑1 (MQ=255)
                                                                                                gTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCCCCCCTATCACTACCCACAACAGATGGGTATAAAGCACCCCTGCGTTGAGTGACATGCCGACACGGATAAGAAACAACCCCAACAGCAAGACTTGAAAggggcg                                                                                                            >  2:764562/1‑236 (MQ=255)
                                                                                                gTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAGAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATc                                                                                                            >  1:810445/1‑239 (MQ=255)
                                                                                                              aaaGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTAACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGAACGCGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGACCGATACCCGTTTccg                                                                                              >  1:316042/1‑238 (MQ=255)
                                                                                                                            caccCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGCCATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAAAAACGATTCCAGATCATGGCGaaaa                                                                                >  2:304654/1‑236 (MQ=255)
                                                                                                                            caccCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGCCATGCCGACAGAGATAAAGAAAAAACCGAGCAGCAAGCCTTTGAAGAGATCGATAACCGTTTCCAGTTCATTGCGctat                                                                                >  1:11671/1‑239 (MQ=255)
                                                                                                                                 tGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc                                                                                     >  2:228795/1‑229 (MQ=255)
                                                                                                                                 tGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc                                                                                     <  1:228795/229‑1 (MQ=255)
                                                                                                                                  gACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCCACCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGACCAGCAAGCCTTTGAAGGGATCAATAGCAGTTTCCAATTCACGGCGATAGTCAAtt                                                                          >  2:564126/1‑239 (MQ=255)
                                                                                                                                  gACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAACGAACAAACCGAGCAGCAAGCCTTTGCAGGGATCGATAGCCGTTTc                                                                                                >  1:476303/1‑217 (MQ=255)
                                                                                                                                        acacGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                    >  1:501297/1‑239 (MQ=255)
                                                                                                                                         cacGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACa                                                                                                                                                     >  1:180681/1‑157 (MQ=255)
                                                                                                                                         cacGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGCTCGAAAACCGTTTCCAGTTCATGGCGAAATTCCCTTTCCCCca                                                                   >  2:588571/1‑239 (MQ=255)
                                                                                                                                                 cAAACTGCATCCGCTCTGAGCTACGCACGCCATAAAATCGCGCCAGCAGATACAGCACGAGAATTTTCACTGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCAGTTTCACATTCATGGCGATCTTCCCTTTCCGCCAGCAGCAc                                                            >  1:439926/1‑238 (MQ=255)
                                                                                                                                                   aaCTGCACCCGCCCAGAGCTCAGCCCGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTCCACCGCCAACAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc                                                         <  1:417663/239‑1 (MQ=255)
                                                                                                                                                      tGCATCCGCTCTGAGCAACGCACGCCACACAAGCGCGCCCGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGc                                                      <  1:764562/239‑1 (MQ=255)
                                                                                                                                                      tGCACCCCCTCTGAGCTACGCCCGCCATACAATCGCGCCAGGAGAAACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGc                                                      <  1:588571/239‑1 (MQ=255)
                                                                                                                                                                     ccacgcccgcCTTACAATCGCGCCAGCATATACAGCCCGAAAATTTCCACCGCCACCAGCACAACCACGCTTAACACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAg                                       <  1:807220/237‑1 (MQ=255)
                                                                                                                                                                     caccgcccgccAAAAAAACGCGCAAGCAGATAAACCACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGa                                        <  1:328297/235‑1 (MQ=255)
                                                                                                                                                                                       cgcgCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGGTTCCAGTTCATGGCGATCTTCACTTTCCGCCAGCAGCACGCCCGCCATAAATGCACCAGACGCCAACAACAGCCCCac                     >  2:42780/1‑238 (MQ=255)
                                                                                                                                                                                         cgcCAGCAGATACAGCACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCCCTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  1:581869/239‑1 (MQ=255)
                                                                                                                                                                                         cgcCAGAAGATACAGCAGGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  1:887849/239‑1 (MQ=255)
                                                                                                                                                                                         cccccgcagTTACACCACGAAAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  2:892888/234‑1 (MQ=255)
                                                                                                                                                                                           ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCTCCAGCAGCACCCCCCCCAAAAAAGTACCAAGCGCAAACACCAGCCCCAacga                  >  2:314156/1‑237 (MQ=255)
                                                                                                                                                                                           ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgccc                  >  1:892888/1‑236 (MQ=255)
                                                                                                                                                                                           ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAACACGCCCGCAATCAAGGAACCGAACGCCCTCGCCACccccccccc                  >  2:557975/1‑233 (MQ=255)
                                                                                                                                                                                                   atcactccGAGAATTTCCCCCGCCACCAGCCCAACCCCGCTTATCTCTACCCACAACAGATGGGTATAAAGCACCCCGTGGTTGAGTGACATGCCGAAAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaaa         <  1:564126/232‑1 (MQ=255)
                                                                                                                                                                                                        cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCACGCCTTTGAAGGGATCTATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCCTCGCCAGCCCCAGCGCATCCATAAACAAt     >  1:193221/1‑238 (MQ=255)
                                                                                                                                                                                                        cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGAGCCATCGACAGCCCCCGCCAATCCATaaaacaa     >  2:130536/1‑234 (MQ=255)
                                                                                                                                                                                                          cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCg  >  1:772197/1‑239 (MQ=255)
                                                                                                                                                                                                          cGAAAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCg  <  1:557975/239‑1 (MQ=255)
                                                                                                                                                                                                             |                                                                                                                                                                                                                                           
AGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCG  >  NC_000913/3479484‑3479924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: