Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,449,045 T→G 25.0% intergenic (+3/+77) chpB → / ← ppa toxin of the ChpB‑ChpS toxin‑antitoxin system/inorganic pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,449,0450TG25.0% 122.7 / 5.7 67intergenic (+3/+77)chpB/ppatoxin of the ChpB‑ChpS toxin‑antitoxin system/inorganic pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (11/36);  new base G (18/2);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 4.33e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCTTTCAGCAGTTCAGGCAGATCGTTAAC  >  NC_000913/4448808‑4449283
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
cTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGgtg                                                                                                                                                                                                                                                    <  1:1850/239‑1 (MQ=255)
cTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGgtg                                                                                                                                                                                                                                                    <  1:207477/239‑1 (MQ=255)
             ggAATGGGGTTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCTGTAGGCCGGATTTAGCGTTCCTTGACATTGGGAAGAAGGCGATGTGCACGGGGTGGTGCTGGGTAATCAGGTGCGGATGATGGATCTGCACGCCCGGTTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTTGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATcg                                                                                                                                                                                                                                       <  2:690030/239‑1 (MQ=255)
                  gtcGCTGTTGTCCCCCATTTCGGTGGGGTGAAATTTTGCCCGTTAGGCCGTATTTAGGGTTCCTTTGCATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGc                                                                                                                                                                                                                                  <  2:544630/237‑1 (MQ=255)
                       tggtggCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTAAGCCGGATTTAGCGTTCCTTTACATTGCGAAGAATGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaa                                                                                                                                                                                                                            <  1:87419/239‑1 (MQ=255)
                         gtggCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAAAGGGGTTATTACGCTTGCAGGGGGGGGGGGGACAAAGGGGGGTTTTTTTTTTGCGGGCaaaaaa                                                                                                                                                                                                                           >  2:47501/1‑239 (MQ=255)
                            gCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                                                                                                                                                       <  1:47501/238‑1 (MQ=255)
                              ccccATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGGGGGGGAAGAGGCGTTATTACGCTTGCAGGCGGGGGGGGGGTAAAGGGGGGTTTTTTTTTCGGGGGATaaaaaaaa                                                                                                                                                                                                                      >  2:513692/1‑236 (MQ=255)
                                           ggcggcAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACCCGCCCGGCTGGCAACGCGTATTGGTCTGGCTGCGGATGAGGGGGGGGAAGAGGCGTTATAAACCTTGCAGGCGGGGGGGGGAAACAGGGGGGTTTTTTTGTTGGGGGAAAAAAAAACACTTCCAAACCGaa                                                                                                                                                                                                        >  2:747765/1‑238 (MQ=255)
                                           ggCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGCTCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAAGCGTTATTACGCTTGACGGCGGGGGGGGGAAAAAGGGGGTTTTTTTATTGCGGGCCAAAAAAAAACCTTaacaacgaaa                                                                                                                                                                                                        >  2:754869/1‑229 (MQ=255)
                                           ggCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTATGCTTGCAGGCGGTGGGGGGATAAGGGGGGGTTATTTATTCCGGGCATAAAAAAAACCCTAACAAAcaaac                                                                                                                                                                                                        >  2:406559/1‑235 (MQ=255)
                                           ggCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTAATAACCGaa                                                                                                                                                                                                        >  1:53968/1‑238 (MQ=255)
                                           ggCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGGGGAAGGGGCGTTATAACGCTTGCAGGCGGTGGGGGGGATAAGGGGGGTTTATTATTTGGGGGGAAAAAAAAAACCTTACCAAcaaaa                                                                                                                                                                                                        >  2:307946/1‑235 (MQ=255)
                                            gCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGGGTTTATTTATCCCGGGCCTAAAAAAAACCTTACTAACCGAAg                                                                                                                                                                                                       >  1:624631/1‑238 (MQ=255)
                                                aaaTGTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAATGCTATGTGCACGGCGTGGTGGTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCg                                                                                                                                                                                                   <  1:191995/238‑1 (MQ=255)
                                                    tttGCTCGTTATGCCGGATTTAGAGTTCCGTTGCAATGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt                                                                                                                                                                                              <  1:642474/239‑1 (MQ=255)
                                                    tttGCCCTTTATGGCGGATTTAGGGTTTCTTTACATTGCGAAGAAGGCGTTTTGCCCGGCGTGGGGCTGGTTAATCAGGTGCGGATGATGGATCTACACGGCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt                                                                                                                                                                                              <  2:67081/239‑1 (MQ=255)
                                                    tttGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt                                                                                                                                                                                              <  1:262038/239‑1 (MQ=255)
                                                    ttggCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt                                                                                                                                                                                              <  1:891988/236‑1 (MQ=255)
                                                                      tttAGTGTTCCTTTACATTGCAGAGAAGGCGGTGTGCCCGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCaga                                                                                                                                                                            <  2:245928/239‑1 (MQ=255)
                                                                          gttttCCTTTTCATTTGGAGATAGGGGGTGTGGACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  2:369950/236‑1 (MQ=255)
                                                                          gctttCTTTTGCATTGCGAATGAGGCGATGTGGTCGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACCCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  2:276592/236‑1 (MQ=255)
                                                                          gctttCTTTTACATGGCGAAGAAGGGGATGTGCCCGGTGTGGGGCTGGTGAATCAGGTGCGGGTGATGGATCTACCCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  2:238322/236‑1 (MQ=255)
                                                                          gctttCCTTTACAGTGGGAAGAAGGGGATGGGCAGGGTGTGGGGCTGGTGAATCAGGGGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  2:624631/236‑1 (MQ=255)
                                                                          gCGTTCCTTTACATTGCGAAGAAGGGGAGTTGCACGGCGTGGTTCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  1:330871/239‑1 (MQ=255)
                                                                            ggtCCGTTACATGGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGGCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  1:442366/235‑1 (MQ=255)
                                                                            gTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                                                                                                                                                        <  1:679862/237‑1 (MQ=255)
                                                                               ccTTTACATTGCGAGGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATTAGGTGGGGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTAGGAAATGGGTGGGTTATTTATGTCGTGGATAAAAACAACCTTTACAACCAGAGACCCGCGTTTAGGGGTATTACGCCCgaaaaaataag                                                                                                                                                                   >  2:586098/1‑234 (MQ=255)
                                                                                     cATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGGATAAGGGGTGTTTTTTTATCGCGGGCCTAAAAAAACCCTTACTAACCGAAACCCGGCGTTCAGGGGTATTACGCCCAGGGAACttatttat                                                                                                                                                               >  1:420547/1‑237 (MQ=255)
                                                                                            aagaagGCGTTGTGCCCGGTGTGGTGTTGGTGAATCAGGGGCGGATGAAGGATCTACACGCCCGGGTGGCAAAGCGTATTGGTCTGGCTGCTGATGAGGTTGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTgcg                                                                                                                                                       <  2:372146/238‑1 (MQ=255)
                                                                                               aagGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGGATAAGGGGGGTTTTTTTTTCGCGGGCATTAAAAAACCCTTTTTAACCAAAACCCGGCGTTTAGGGTTTTTACCGCAGAAAAAATTATTTAtttttttgggcgc                                                                                                                                                   >  2:852918/1‑232 (MQ=255)
                                                                                                               cGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGAGGTGGAAGAGGCGTTATTACGATTGCAGGCGGGGGGGGAATAAGGGGGGTTTATTTATCGCGTGCATAAAAAAACCCTTCATAACAGGAGACCGGGGTTCAGGGGTAATAACCCAAAAGAACTTATTTTTTTCTTGTGCGCTCTGAGGAGGAAACGag                                                                                                                                  >  2:29947/1‑238 (MQ=255)
                                                                                                                  ggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCCTAAAAACTTATTTATTCTTTTCGCGCTCGACAGAGGGAACGAttt                                                                                                                                >  1:298831/1‑238 (MQ=255)
                                                                                                                  ggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGTGATTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGATCAGGGTTATTAAGCCAGAAGAACTTCTTTATTCTTTGCGCGCTCGAAGGAGGCAACGAttac                                                                                                                               >  1:482872/1‑237 (MQ=255)
                                                                                                                  ggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGGGGAATAAGGGGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCCGAAGAACTTATTTATTCTTTTCGCGCTCGAAGGAGGCAACGATTTc                                                                                                                               >  1:23959/1‑239 (MQ=255)
                                                                                                                  ggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTAAGCTTGCAGGCGGGGGGGGAATAAGGTGTGTTTATTTAACCGGGGCATAAAAAAACCCTTACTAAACGAAGCCCGGGGGTGAGGGTTGTTTAGCACGCAGAACTTTTTTTTTCTTTTAGCGCCCGAGAGGGGGGACGAttta                                                                                                                               >  2:298729/1‑238 (MQ=255)
                                                                                                                         gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGGATAAGGTGTGGTTATTTATCGCGGGCATAAAAAAAACCTTACTAACCGAAGCCCGGCGTTCAGGGGTATTAACCCAGAAGAACTTATTTAATCTTTGCGGGCCCGAAGGAGGCAACGATTTCAACTTTa                                                                                                                        >  1:303803/1‑239 (MQ=255)
                                                                                                                         gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGGGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAACCCGGCGTTCAGGGTTATTACGCCAGAAAAACTTTTTTATTCTTTTCCGGCTCGAAGGAGGGAACGATTTCAGCTTTa                                                                                                                        >  1:6935/1‑239 (MQ=255)
                                                                                                                         gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGGTATTACGCCAGAAGAACTTATTTTTTCTTTTCGCCCCCCAAGGAGGCAAAGATTTTAACtttt                                                                                                                        >  1:508751/1‑238 (MQ=255)
                                                                                                                         gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCGGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGGGTTTTTTTATTGCGGGCCTAAGAAAACCCTTACTAACCCAAGCCCGTGGGTTAGGGGTATTACGACCACAGAATATTTTTATTCTTTTTGCGCCGGCAGGAGGCAACGAttttgactttt                                                                                                                        >  2:25877/1‑231 (MQ=255)
                                                                                                                           gAATCAGGTGCGGATTATGGATCTACACGGCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAg                                                                                                                       <  1:763623/238‑1 (MQ=255)
                                                                                                                              tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGGGGGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCCGGGGTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGca                                                                                                                     >  1:658724/1‑236 (MQ=255)
                                                                                                                                   tGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGGGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGGTATTTCGCCCGAAGAACTTATTTATTCTTTTCGCCCCCGCAGTAGGCAACGATTTCGGCTTTATCGGGTTCtt                                                                                                             >  1:805093/1‑238 (MQ=255)
                                                                                                                                             ggATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTccc                                                                                                    <  1:852686/238‑1 (MQ=255)
                                                                                                                                             ggATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCa                                                                                                   <  1:707205/239‑1 (MQ=255)
                                                                                                                                               aTCTACACGCCCGGCTGGCAAAGCGGATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAg                                                                                                                             <  1:231283/212‑1 (MQ=255)
                                                                                                                                                 ttacctgCCGGGCTGGGAAAGTGTTTTGGTCTGGCTGGGGATTGGGTGGTGTAAGAGGCGTTATTACGGTTGCAGGCGGTGGTGGATTAAGGGTTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACTATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCt                                                                                               <  2:88594/233‑1 (MQ=255)
                                                                                                                                                  tACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGGGGAATAAGGGGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAAATTATTTATTTTTTTCGCGCTCTAAGGGGGCAACCATTTCAGCTTTTTCTGCTTTCTCGGTTTTCCCAccc                                                                                                >  2:615773/1‑238 (MQ=255)
                                                                                                                                                         ccGGCTGGCAAAGCGTATTGGTCTAGCTGCGGATGCGGTGGTGGAAGAGGCGTTATTACGCTTGAAGGCGGGGGTGGAATAAGGGGGGTTTATTTATCGCGGGTATAAAACAACCCTTCCTAACCGAAGCCCGGCGTTTAGGGGTATTACACCAAAAGAACTTTTTTCTTCTTTGACCGCCCCACGGGGGCAACGCCTTCACAGTTAGCGTGTTCTTCGTTTTCCCAcccccccacctt                                                                                       >  2:446538/1‑230 (MQ=255)
                                                                                                                                                                 cAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTc                                                                                                                               >  1:571702/1‑192 (MQ=255)
                                                                                                                                                                 cAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTc                                                                                                                               <  2:571702/192‑1 (MQ=255)
                                                                                                                                                                       gTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGcc                                                                          <  1:274067/237‑1 (MQ=255)
                                                                                                                                                                           tGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGGGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTc                                                                    <  1:406559/239‑1 (MQ=255)
                                                                                                                                                                              tCTGGCTGGGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGa                                                                  <  1:852918/238‑1 (MQ=255)
                                                                                                                                                                              tCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTGATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGa                                                                  <  1:121632/238‑1 (MQ=255)
                                                                                                                                                                                  gCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTAATAAACGAAGCCCGGCGTTTAGGGGTATTACTGCAACAGAACCTATTTTTTCTTTGGGCGCTCGGAGGTGGGAACGATTTCAGCTTTAACGGCTTTTGCGTTTTTCCCACCTTCAACATTTACCCACTTTGCTTTTTctggggct                                                             >  2:593013/1‑232 (MQ=255)
                                                                                                                                                                                  gCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGGGGTGGAATAAGGTGGGGTTATTTATCGCCGGCCTAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGGTATTACGCCAAAAGAACTTATTTATTCTTTTCGCGCTCGAAGGGGGACAACATTTTACCTTTAGCGGCTTCTGACGTTTCCCCACCCTCCCCTCTTACCCACCTTCCCtttttgtgggct                                                              >  2:121632/1‑232 (MQ=255)
                                                                                                                                                                                        gATGAGGTGGTGGAAGGGGCGTTATTACGCTTGAAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTa                                                        <  1:298643/238‑1 (MQ=255)
                                                                                                                                                                                                 gtggAAGAGGCTTTATTACGCTTGAAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCgaa                                               <  1:37711/238‑1 (MQ=255)
                                                                                                                                                                                                 ggggAAGCGGCGGTAGTTCGGTTGGAAGCGGTGGGTGAATAAGGTGTGTTTAATTATCGGCGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCgaa                                               <  1:684432/236‑1 (MQ=255)
                                                                                                                                                                                                         ggCGTTATTACGCTTGCAGCCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGACGAAGCGAGc                                      >  1:820957/1‑239 (MQ=255)
                                                                                                                                                                                                              ttttagtcTTGGAGGGGGTGGTGGAATAAGGTTTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGAttt                                 <  2:23959/232‑1 (MQ=255)
                                                                                                                                                                                                              tattaCGCTTGCAGGCGGTGGTTGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGAttt                                 <  1:581281/239‑1 (MQ=255)
                                                                                                                                                                                                                   agCTTTCAGGCGGTGGTGGAATTGGGGGTGTTTATTTAACGGGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCt                            <  2:107721/238‑1 (MQ=255)
                                                                                                                                                                                                                                ggtggtGGAATAAGGTTTGTTTTTTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTAGTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCTTTCAGCAGtt                  <  2:371032/238‑1 (MQ=255)
                                                                                                                                                                                                                                       gggAATAAGGTGTGTTTATTTAGCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTGCACCCACTTGCCTTTTTCGAGGGCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCTTTCAGCAGTTCAGGc             <  1:664551/237‑1 (MQ=255)
                                                                                                                                                                                                                                        tgAATAATGTGGTGTTTTTTATCCGGGGAATAAAAAAACCCTTGCTAACCGAAGTCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCTTTCAGCAGTTCAGGCa            <  2:298831/237‑1 (MQ=255)
                                                                                                                                                                                                                                        ggAATAAGGTGTTTTTATGTATCGGGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCTTTCAGCAGTTCAGGCa            <  1:25877/238‑1 (MQ=255)
                                                                                                                                                                                                                                          aaTAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCCCTTTCCTTTTTCGAGGTCTTTGGAGTGCTCGAAGAAGTAAGCGATTTTCGCTTTCAGAGGTTTAGGAAGAt         >  2:204098/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 gtgtgtTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTTACCCACTTGCCTTTTTCGCGGTCTTTGTAGTGCTCGCAGAAGTGAGCGATTTGCGCTTTCAGCAGTTCAGGCAAATAGTaaa   >  2:244866/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  tgtgtTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTTCCTTTTTTGAGGCCTTTGTAGAGCTCGAAGAAGTGAGAGATTTGCGCTTTAAGCGGATCGGGGAGAGCggttaa  >  2:109997/1‑234 (MQ=255)
                                                                                                                                                                                                                                                  tgtgtTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGGGAGCGATTTGCGCTTTCAGCAGTTCAGGCAGATCGTTAAc  >  1:15265/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  tgtgtTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGGTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAATGAGGGGTTTGCGCTTTCAGCAGTTAAGGCAGATGGTGAAc  >  2:373001/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    
CTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGGGCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAACGATTTCAGCTTTAGCGGCTTCTGCGTTTTCCCAACCTTCAACTTTCACCCACTTGCCTTTTTCGAGGTCTTTGTAGTGCTCGAAGAAGTGAGCGATTTGCGCTTTCAGCAGTTCAGGCAGATCGTTAAC  >  NC_000913/4448808‑4449283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: