| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,874,824 | C→G | 9.3% | T251T (ACG→ACC) | yeaM ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,874,824 | 0 | C | G | 9.3% | 107.3 / 2.2 | 37 | T251T (ACG→ACC) | yeaM | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (10/23); new base G (2/2); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCTGAACTGGAAATCACTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCC > NC_000913/1874589‑1875052 | ttttttgattGGAAATCACTTGGGGCGTGTGGGGTAGGCGTGATTGTCCCCGGGCTGGGTGGGCGGGGGGTGACGTTAATGGGCAGCCAGCCGCATCTGGTCGGCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt < 2:288516/230‑1 (MQ=255) ttCTGAACTGGAAATCGCTGGCGGGGATTGCAGTAGGCGTTATTGTCTCCTGGCTGGGTGGGCGCGGCGGGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt < 1:236865/238‑1 (MQ=255) ggtaatcgttggtggCGATTGGGGTAGGCGGGATTTTCGCCCGGCTGGGTGGGGGGGGGGGGACGTTAATGGGCAGCCAGCCGGGACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGGTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc < 2:379794/230‑1 (MQ=255) ggAAATCCCTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCTCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc < 1:122793/239‑1 (MQ=255) gAAATCACTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTGGTTAATCTCGCAATATCTCGGCCTGGGGTTTGGCCCAgg > 1:405727/1‑237 (MQ=255) aacttcTGGTGGGGATTGGGGTGGGCGGGGTTGTCTCCTGGCTGGGGGGGCGGGGCGGGACGGTAATGGGCATCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCCCTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt < 2:346902/233‑1 (MQ=255) cTGGTGGCGTTTGCAGTAGGCGTGATTTGCCCCGGGCGGGGGGGGGGCGTCGGGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa < 2:818965/239‑1 (MQ=255) gggggCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa < 1:250058/236‑1 (MQ=255) ggggCGTTTGATGTGGGCGGGTTTGGCTCCTTGCTGGGGGGGCGAGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGGTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa < 2:290484/236‑1 (MQ=255) tgtgggttGCAGGGGGCGTTGTTGTCCCGTGGCTGGGGGGGGGGGGGGTGGCGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGTTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa < 2:780860/233‑1 (MQ=255) tgggtgttGCAGTGGGGGTTATTGTCGCCTGGGTGGGGGGGGGCGGGGGGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGGGTTTGCCCCAGGCCTTTCTTAAACa < 2:405727/233‑1 (MQ=255) ggttttcgttgggCGTGTTTTTCTCCTGGGTGGGGGGGGGCGGGGTGGCGTTAATGGGAAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATa < 2:365505/227‑1 (MQ=255) ggCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGTTTTCTCTGCTTGGGGGGAAACCGTAGTTAATCTCGCCATTAATCGCCCTGGCGTTTGCCCCAGGCCCTTCTTAAAACTAATAAAAAAAGcc > 2:236540/1‑239 (MQ=255) tcCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCCCTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGGTTGTGGGGAAACAGTCGTTAATCTCGCACTATATCGGCCTGGCGCTTGCCCCAGGCCTTTCTTAAACAAATGAAAAAAGGGCgttgtttacaca > 2:310024/1‑233 (MQ=255) tcCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCa > 2:808719/1‑239 (MQ=255) tcCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGGTT‑CGCCGCGTACCGGTTGGGCCGCTTATTGCCGCGGGGCTGGTTTCGCTGATTGTGGGGAAAACGTGGTTAATCTCGACATATATCCGCCTTGGGTTTGCCCCAGGCCTTCTTTAAACATAGTAATAAAAGCGGTGGTTGAAtta > 2:653474/1‑237 (MQ=255) gtcgggccGTTTATGGCCACCCCGCCGCAAGTGGGCGCCGGGCTGGTGGTAGGCATTGTTTTAGGCGTAGCGCTGTTT‑CGCGGTGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa < 2:774522/232‑1 (MQ=255) ggcgTGAAGTTAATGGGCAGCCAGCCAGAACTGGTCGCCGTGCTGGTTGTAGGCATTGTTTTAGGCGTAGCTCTTGTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa < 1:418244/239‑1 (MQ=255) aCGTTAATGGGCCGGCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt < 1:838059/238‑1 (MQ=255) ccgccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTTTTTTAGGCGTAGCGCTGTTT‑CGGGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTAt < 1:120177/236‑1 (MQ=255) cagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGcc < 1:808719/238‑1 (MQ=255) accggggcGCCGGGCTTGTGGTAGGCCCTTTTTTGGGTGTAGCG‑TGTTTGCGGGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACt < 2:519708/231‑1 (MQ=255) tGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATTGCCTTTCACTAAt > 1:419252/1‑238 (MQ=255) cGGGCTGCTGGTAGGCCCTGTTTTAGGCGTAGCGTTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAg < 1:310024/239‑1 (MQ=255) tctgctgGTAGGCACTGTTTTAGGCGTAGCGGTTGTG‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAgcg < 1:286937/237‑1 (MQ=255) ggcctttttttGCCGGGGGTCTGGTTTCGCTGATTGTGGGGAAACGATGGTTAATCTCGAAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATGCCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCtt < 2:794711/230‑1 (MQ=255) cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCCCCATAATCAGCTGTAACTGCTGCCGCCACTGGCGAAAACTCAACCCCGTCCCTTTTACTTTCatccgg > 2:877831/1‑233 (MQ=255) gggTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCACCTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATAAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACCTTCAATCCGGc > 2:530767/1‑239 (MQ=255) gtttGGTTTCGGTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCGTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGt < 1:592668/213‑1 (MQ=255) gtgGGTAAACAGTTGTTTATCTCGCAATATCTCGGCCTGGGTTTTTCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTTAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCCCTAATCCCTGAAGCGCCATAATCAGCTTTAACTGCTGACGCCCCTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATc < 2:539762/239‑1 (MQ=255) cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATaa < 1:614692/134‑1 (MQ=255) cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATaa > 2:614692/1‑134 (MQ=255) cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCCCTGGCGAAAACTCAACCCCGCCTCCTTTACTATCAGTCGGCCAAGATTGCGTTCACTCATCCCCAAAAAGACACCCAAttcc > 2:144351/1‑236 (MQ=255) cGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCTTTCCTTTTACTATCAGTCGGGCACGATTGCGTTCACTCACCCCAAAAACGCCAGCCCATTGc > 1:403698/1‑238 (MQ=255) atCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGGTGTTGAATCATGCCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGcc < 1:236540/239‑1 (MQ=255) gCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGc > 2:184693/1‑189 (MQ=255) gCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGc < 1:184693/189‑1 (MQ=255) | TTTCTGAACTGGAAATCACTGGTGGCGATTGCAGTAGGCGTGATTGTCTCCTGGCTGGGTGGGCGCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTT‑CGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAATGCC > NC_000913/1874589‑1875052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |