Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,214,6600AG24.3% 114.2 / 12.4 75E538E (GAA→GAG) rpoDRNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base A (31/22);  new base G (2/15);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAC  >  NC_000913/3214522‑3214802
                                                                                                                                          |                                                                                                                                              
cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGcc                                                                                                                                            >  1:557231/1‑143 (MQ=255)
cAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGAAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATGTCATCGAGGAGACCCCCCTGGGCAACCCGCTGGACTCCGCAACCACCCAAAGcc                                                                                                                                            >  2:1085732/1‑143 (MQ=255)
   gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGCAAGCCtgc                                                                                                                                         >  1:193197/1‑143 (MQ=255)
   gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGACAGCCtgc                                                                                                                                         >  1:244379/1‑143 (MQ=255)
      ccGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGCACCACATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCCACCACCGAGAGCCggcgcc                                                                                                                                      >  2:167182/1‑141 (MQ=255)
        gCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACGACCCTCGAGCGGCCGCAGGATTCTGCGACCACCGAAAGCCggcgtgcg                                                                                                                                    >  2:677445/1‑143 (MQ=255)
         cAAAGTGCTGAAGATCGCCAAAGAGCCAACCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCgg                                                                                                                                   <  1:604617/143‑1 (MQ=255)
            aGTTCTGAAGATCTCCAACGAGCCACTCTCCATGGACACGCCGATCGGTGATGATGCAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCaa                                                                                                                                <  1:561270/143‑1 (MQ=255)
                 tGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGCGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCac                                                                                                                           >  2:12083/1‑143 (MQ=255)
                 tGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCGGCGTGCGGCCACggcg                                                                                                                           >  2:611682/1‑140 (MQ=255)
                      tgcGCCAGAGTGCCATTCTCCAGGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCAGCGAGGAGACCACCCTTGTGTTGCTGAATGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                      <  2:1176553/141‑1 (MQ=255)
                      ctCGCTAAAGATCCTATCTCACTGGACACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTTGTTTTTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                      <  2:4146/142‑1 (MQ=255)
                      aTCGCCAAAGAGCCAATCTCCATGGAAACGCCGTTCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGt                                                                                                                      <  1:1120092/143‑1 (MQ=255)
                        cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGAATTTTAGCGTGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgc                                                                                                                    >  1:1297453/1‑143 (MQ=255)
                          ccAAAGAGCCACCCTCCATGGAAACGCCGATCGGTGATGATCCAGATTCGCCTCTCGGCGATTTCATCGAGGATACCACCCTCGAGCTGCTGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                                  <  1:230192/143‑1 (MQ=255)
                          ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGACAGCCTGCGTGCGGCAACGCCCGCCGTgctg                                                                                                                  >  1:1309470/1‑143 (MQ=255)
                          ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCAGCGAGCGGCGACGCACGACATgccg                                                                                                                  >  2:78650/1‑141 (MQ=255)
                          ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATGTCATCGAGGCTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctg                                                                                                                  >  1:395998/1‑143 (MQ=255)
                          ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGAAGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAACCACGAGCTGCCGCTGGATCCTGCGACCACAGAAAGCCGGCGTGCCGCCACGCgcgaccggccg                                                                                                                  >  2:137817/1‑137 (MQ=255)
                            aaaGAGCCAATCTCCATGAAAACGCCTATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTgctggc                                                                                                                <  2:1083609/143‑1 (MQ=255)
                             aaGAGCCTATCTCCATGGTTCCGCCGATCGGTTCTGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGTGCTGTCTAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggct                                                                                                               <  2:834733/143‑1 (MQ=255)
                              agagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTTTTGTTGCCGAAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTgctggctg                                                                                                              <  2:1059338/143‑1 (MQ=255)
                                     agctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGAc                                                                                                       <  2:665437/141‑1 (MQ=255)
                                        tcCATGGATGCGCCGATCGGTGATTATGAAGTTTCTCATCTGGGGGATTTCATCGAGGATACCACCCTTGTGCTGCCGCTGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgc                                                                                                    <  2:105348/143‑1 (MQ=255)
                                        tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACGCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgc                                                                                                    >  1:376508/1‑143 (MQ=255)
                                         ccctcgAAGCGCCGATCGGCGATGATCAACATTCGCATCTGGGGGATTTCACCGAGGATCCCACCCTCGAGCTGCCGCTGGCTTCTGCGACCACCAATATCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACcgcg                                                                                                   <  1:628378/138‑1 (MQ=255)
                                            tGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGACACCACCCCCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGt                                                                                                <  1:129575/143‑1 (MQ=255)
                                               gaaCGCCGAGCGGTGGTGATGAGGATTCGCATCTGGGGGATGTCATCGAGGATACCACCCTCGATTTGTCTAAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                             <  2:829453/142‑1 (MQ=255)
                                               aagcGCCGATCGGTTATGATGAAGATTCGCATCTGGGGGATTGCATCGAGGATACCACCCTCGTGCTGCCGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                             <  2:1340679/140‑1 (MQ=255)
                                               aagcGCCGAGCGGGGATGCTGAAGAGTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGaa                                                                                             <  2:1227205/140‑1 (MQ=255)
                                                     cGATCGGTGATGATGAAGATTCGCATCTGGGGGTTTTCATCGAGCATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTCCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCaaaa                                                                                       <  1:1111435/143‑1 (MQ=255)
                                                        tCGGTGATGATGAAGATCCGCATCGGGGGGATTTCATAGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGtt                                                                                    <  1:1158478/143‑1 (MQ=255)
                                                         cGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGCGACGCACAAGTTc                                                                                   >  1:893195/1‑143 (MQ=255)
                                                                 atgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATg                                                                           >  1:210297/1‑143 (MQ=255)
                                                                  tgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGc                                                                          >  1:259075/1‑143 (MQ=255)
                                                                    caGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                        <  1:84824/142‑1 (MQ=255)
                                                                    aaGATTCGCATCCGCGGGCTTCCATCGATCTTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGt                                                                        <  1:91123/143‑1 (MQ=255)
                                                                        ttCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                                    <  1:64652/143‑1 (MQ=255)
                                                                        ttCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCg                                                                    <  1:1111029/143‑1 (MQ=255)
                                                                              tCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGCATCg                                                              >  1:1237468/1‑143 (MQ=255)
                                                                               cTGGGGGATTTCATCGAGGATACCACCCGCGAGCTGCCGCTGGACTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCAAGACGTGCTGGCTGGCCTGCCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGa                                                             <  1:1147384/143‑1 (MQ=255)
                                                                                tGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCATGCGTGCGGCACCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGat                                                            >  1:1100378/1‑143 (MQ=255)
                                                                                  ggggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGatat                                                          <  2:1297453/143‑1 (MQ=255)
                                                                                    ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGCAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGa                                                        >  1:560849/1‑143 (MQ=255)
                                                                                    ggATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGa                                                        >  1:1108673/1‑143 (MQ=255)
                                                                                       tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGGTGTCCGCACCGCCCGTGAAGCAAAAGGTCTGCGTATGCGTTTCGGTATCGATATGAcca                                                     >  1:329676/1‑143 (MQ=255)
                                                                                        ttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCCCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAacac                                                    <  1:1087375/143‑1 (MQ=255)
                                                                                            tCGAGGATCCCACCCTCGTGTTTCTGCAGGATACGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                                <  2:990980/143‑1 (MQ=255)
                                                                                            tCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGAc                                                >  1:1319618/1‑143 (MQ=255)
                                                                                               aGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCgacgac                                             >  1:634228/1‑143 (MQ=255)
                                                                                                    gccaccCTCGGGCGGCCGCTGGATGCTGCGACCACCGAAGGCCTGCGGGCGGCAACGCACGACGGGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCt                                        <  1:1004956/142‑1 (MQ=255)
                                                                                                     ccaccCTCTGGCTGTCGCAGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTg                                       <  2:650751/143‑1 (MQ=255)
                                                                                                      caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTCTGCGTGTGGGTATCGATATGAACAGCGACCACGCGGTgg                                      >  1:8894/1‑143 (MQ=255)
                                                                                                      caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGGTTCGGTATCGATATGAACGCCGACTACACGCTgg                                      >  1:1353278/1‑143 (MQ=255)
                                                                                                      caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCATGACCGCCCGTGAAGCAAAAGTTCTGCGTAGGCGTTTCGGTATCGCTAAGACCACCGACGACACGCCgg                                      >  2:93186/1‑143 (MQ=255)
                                                                                                       accCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATCTGGACACCGACTACACGCTGga                                     >  1:362427/1‑143 (MQ=255)
                                                                                                        cccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGgaa                                    <  1:498393/143‑1 (MQ=255)
                                                                                                           tCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAGAGTTCTGCGTATGCGTTTCGGTATCGATATGAGCACCGACTACACGCTGggagga                                 >  1:698578/1‑141 (MQ=255)
                                                                                                               tctgctgATGGATTCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  2:482210/142‑1 (MQ=255)
                                                                                                               gCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACGCCGACTACACGCTGGAAGAAGTgg                             >  1:1361107/1‑143 (MQ=255)
                                                                                                               gCTGCCGAGGGATTGTGCGACCACCGACGGCCTGCGTGCGGCAACGCACGGCGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  1:1358205/143‑1 (MQ=255)
                                                                                                               ccTGCCGCTGGATCCTGCGCCCCCCGCAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                             <  1:12083/142‑1 (MQ=255)
                                                                                                                  gCCGCTGGATTCTGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCACGCGTGAAGCAAAAGCACTGCGTATGCGTTTCGGTATCGACATGAACACCGACTACACGATGGaagaagaaggga                          >  2:263780/1‑137 (MQ=255)
                                                                                                                   ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaa                         <  1:918716/143‑1 (MQ=255)
                                                                                                                    tgccggATGCGGCGACCACCGAGAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                        <  2:1177091/139‑1 (MQ=255)
                                                                                                                      cTGGATTCTGCGACCGCCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACa                      <  1:917810/143‑1 (MQ=255)
                                                                                                                         ggttCTGCGACCACCGATAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATGGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGtt                   <  2:930618/141‑1 (MQ=255)
                                                                                                                          aTTCTGCGACCACGGAACGACGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc                  <  2:7510/143‑1 (MQ=255)
                                                                                                                             ccgCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACTTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc               <  1:553019/141‑1 (MQ=255)
                                                                                                                             cTGCGACGTCCGAAAGGCTGCGTGCGGCAATGCACGACGTGCTGGCTGGCCGGACCGCGCGTGAAGCAAAAGTTGTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc               <  1:1159381/143‑1 (MQ=255)
                                                                                                                              tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGAAGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGATGTGCGTATGCGTTTCGGTATCGATATGAACACCGAATACACGCTGGAAGAAGTGGGTCAAAAGTTCGACg              >  1:635904/1‑143 (MQ=255)
                                                                                                                                 gACCACCGGGAGCCGGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa           <  2:949522/143‑1 (MQ=255)
                                                                                                                                     accGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGCTACCcg       >  2:266997/1‑143 (MQ=255)
                                                                                                                                       cGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAACGTTCTTCGTCTGCGTTTCGGTATCGCTGTGCACCCCGCCTCCACGCTGACGCAAGTGGGTAAACCGGTCGACGCTCCCcgcg     >  2:257329/1‑143 (MQ=255)
                                                                                                                                        gAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGAGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACCTTACCcgcgc    >  2:833563/1‑142 (MQ=255)
                                                                                                                                         aaaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCGTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACGACACGCGGGAAGAAGTGGGTAAACAGTTAGGCGTTAACGGCGaa   >  1:870689/1‑143 (MQ=255)
                                                                                                                                          aaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAc  <  2:395998/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
CAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAC  >  NC_000913/3214522‑3214802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑