| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,214,415 | A→C | I457L (ATT→CTT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,214,415 | 0 | A | C | 98.4% | 195.3 / NA | 64 | I457L (ATT→CTT) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (26/37); minor base G (0/1); total (26/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAG > NC_000913/3214277‑3214553 | ggaggggtgaAAGCGGTTGATAAATTCGAATTCCGCCGGGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTga < 2:852687/136‑1 (MQ=255) gTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATGACGGTGCATATGCTTgag > 1:367115/1‑143 (MQ=255) atccgTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATcaaca < 1:1113798/139‑1 (MQ=255) aGCGGTTGATAAATTCGAATACCGCCGTGGTGACAAGTTCTCCACCGACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATcaaca < 2:1231372/143‑1 (MQ=255) gggcttGATAAATTCGTATACCGCCGTGGTTACAAGTTATCCACCTACGCAACGTGGTGGATCCGTCAGGCGATGAACCGCTCTATCGCGGATCAGGAGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATcaacaa < 1:43245/139‑1 (MQ=255) gCGGTTGATAAACTCGAATCCCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATcaacaa < 2:766991/143‑1 (MQ=255) ggTTCATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGc < 2:790501/143‑1 (MQ=255) tAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAATCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCAACATCCGTCTTCCGGTGCCTATGCTTGAGACCAACAGCACGCTccccc > 2:1111435/1‑139 (MQ=255) aaaTTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCg > 1:633190/1‑143 (MQ=255) aaaTTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCg > 1:843941/1‑143 (MQ=255) ctACCGCCGTGGTGACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTtctc < 1:1396669/142‑1 (MQ=255) tACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCg < 1:233277/143‑1 (MQ=255) actgccgTGATTACACGCGCTCCACCTCCCCAACCTGGTGGATCCGTCCGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGGGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGc < 2:554312/140‑1 (MQ=255) cgccgTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCa > 1:8405/1‑143 (MQ=255) gTGGTTACAAGTTCTCCACCTACGCAACCTGGTCGATCCGTCAGGCGATCACCCGCTCTATCGCGGAGCAGGCCCGCACACCCCGTCTTCCGCTGCCTATGCTTGAGAACATCAACAAGATGAACGGTATTTACCGCCAGacg > 2:553019/1‑141 (MQ=255) ggTTACAACTCCTCCACCTACGCAACCTGGTGGCTCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGAtgct < 1:487246/143‑1 (MQ=255) gTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCCGGTGCTTTTGATGACTTGCAGTGATTAATATCGTTGCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGAtgctg < 2:642974/143‑1 (MQ=255) gTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGCCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGAtgctg < 1:1210152/143‑1 (MQ=255) ttACAAGTTCTCCACCTACGCGACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGAtgctgc < 2:914554/143‑1 (MQ=255) ttCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGAt > 1:1305269/1‑143 (MQ=255) ttCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGAt > 1:794528/1‑143 (MQ=255) ctcCACCTACGCAACCTGGTGGATTCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATgg < 2:9486/143‑1 (MQ=255) ctcCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATgg < 2:164037/143‑1 (MQ=255) cTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTg > 1:775345/1‑143 (MQ=255) aCGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCAGTATTGCGGTGCATATGCTTGAGACCATCAACCAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAAATGGACCGTGaa > 2:1158478/1‑143 (MQ=255) cGCAACCTGGTGGCTCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAAc < 1:274803/143‑1 (MQ=255) tgactcTGGTGGATCCGTCAGGCGATCACCCGCTCGAGCGCGGATCGGGCGCGCACCTTCCGTATTCCGGTGCATATGCTTGAGACCATTAACAAGCTCAACCGCATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAAcc < 2:377141/138‑1 (MQ=255) gCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAGGCTCAACCGTATTTCTCGCCCGATGCTGCACGCGATGGGCCGTGCAcc > 1:930618/1‑143 (MQ=255) aaCCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGa < 1:486333/143‑1 (MQ=255) ccTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATGCGTATTCCGGTGCGTATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAAACgcag > 2:129575/1‑140 (MQ=255) cTGTTGTATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGc < 2:721455/143‑1 (MQ=255) tggtggATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGACCCGACggc > 1:1116749/1‑141 (MQ=255) tggtggATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGcc > 1:650751/1‑143 (MQ=255) tggtggATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGcc < 1:1301453/143‑1 (MQ=255) gtggATCCGTCAGGCGGTCACCCGGTCTATCCCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCgg < 2:353960/143‑1 (MQ=255) tggATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGAGAACCGACGCCGga > 1:154948/1‑143 (MQ=255) ccGGCAGGCGATCACCCGCGCTAACGCGGATCAGGCGCGCACCATCCGTATTCCGGGGCATAAGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAAc < 2:1251400/143‑1 (MQ=255) ccGGCAGCCGATCTCCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAAc < 2:989389/143‑1 (MQ=255) ccGCCAGGCCACCACCCGCTCTCTCGCTGATCAGTCCCCTCCCATCCGTATTCCGGTCCATATGCTTGAGCCCCTCCACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAAc < 2:469555/143‑1 (MQ=255) gTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGCTGCTGCCCGCGCTGGGCCGTGCACCCACGCCGCAAGAACTg > 1:1227205/1‑143 (MQ=255) tCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTgg < 1:966839/143‑1 (MQ=255) ccAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTgg < 2:459790/142‑1 (MQ=255) cAGGCGATCACCAGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTCCATAGGCTTGAGACAATCAACGAGCGCAAGAGGAGTTATCACCAGATGCGGGAAGCGATGGGCAGAGACACGGCGCGGGAAGGACAggg > 2:1147384/1‑142 (MQ=255) cctcGCTCTATCGCTGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGAACATCAACAAGCTCAACCGAATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTAt < 1:1147352/140‑1 (MQ=255) aCCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCGACAAGCTCAACCGTATTTCTCGCCCGATGCTGCACGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGGTGAACGTAt > 1:785071/1‑143 (MQ=255) ctctGTCGGGGATCAGGCGCGCACGATCCGTATTCCGGGGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGa < 1:319968/143‑1 (MQ=255) gctATCGCGGTTCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGAt < 2:509942/142‑1 (MQ=255) tctCGCGGATCAGGCGCGTACCGGCCGGCTTCCCGTGCATATGCTTGAGCCCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATCGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGc < 2:349301/141‑1 (MQ=255) tCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCg > 2:123181/1‑143 (MQ=255) cgGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCGCAACCGTATTCCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGaa < 1:80027/143‑1 (MQ=255) ccgATCAGGCGCGCACCATCCGTATCCCGGTGCATATGCTTGAGACCAGCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAACAACTGGCTGAACGTATGCTGATGCCGGaa < 1:80035/141‑1 (MQ=255) gATCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACACGCGCAACCGTATTTCTCGCCAGAGGCTGCAAGAGATGGGCCGCGAACCGACGCGGGAACAAACGACTGAACGCACGCAGCTGCCgggcga > 2:467712/1‑139 (MQ=255) tCAGGCGCGCACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACa > 1:1176553/1‑143 (MQ=255) gcgcgcACCATCCGTATTCCGGTGCATATGCTTGAGGCCATCAACAAGCTCAACCGTATTTCTCGCCAGCTGCTGCAAGAGATGGGGCGTGAACCGACGCCGGAAGCACTGGCTGAACGCCTGCTGCGGCCGGACGACAAGac > 2:91123/1‑142 (MQ=255) gcgcgcACCATCCGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAATAACGAGCTGAACGGATGCCGATGCCGGAAGACAGGaa > 2:84824/1‑142 (MQ=255) gcgcgcACCATCCCTATTCCGGTGCATATGCCTGAGACCATCAACGAGCTCACCCGTATGTCTCGCCAGATGCTGCAAGATATGGGCCGTGAACCGACGCCGGAAGAAATGGCTGAACGTATGCTGATGCCGGAAGACAAGAt < 1:402148/143‑1 (MQ=255) ccGTATTCCGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGc < 2:843941/143‑1 (MQ=255) atgccGGTGCATATGGTTGAGACCATCAGCGAGCTGGACCGGAGTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGaa < 2:393249/140‑1 (MQ=255) ccGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGAt > 1:990980/1‑143 (MQ=255) cGGTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATc < 2:1305269/143‑1 (MQ=255) cGGTGCAGAGGCTGGAGACCATCAACAAGCTCAGCCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGGGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATc < 2:1191465/143‑1 (MQ=255) ggTGCATATGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCg < 2:8405/143‑1 (MQ=255) tatGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCCAGAGATGGGCCGTGGACCGACGCCGGAAGAACCGGCTGACCGTATGCTCATGCGGGGAGACAAGAACCGCGAAGTGCCTATCATCGCCaaac > 2:1399839/1‑142 (MQ=255) tatGCTTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACCAGATCCGCAGAGTGCCGAAGAGCGCCCAag > 1:130164/1‑143 (MQ=255) | GGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACCATCAACAAGCTCAACCGTATTTCTCGCCAGATGCTGCAAGAGATGGGCCGTGAACCGACGCCGGAAGAACTGGCTGAACGTATGCTGATGCCGGAAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAG > NC_000913/3214277‑3214553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |