Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,020,956 A→G intergenic (‑37/‑182) sulA ← / → sxy SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,020,9560AG98.7% 242.8 / ‑4.6 80intergenic (‑37/‑182)sulA/sxySOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (42/36);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATC  >  NC_000913/1020817‑1021090
                                                                                                                                           |                                                                                                                                      
cACTGATATGCCCCGCTGGAGTGTTGTCCGTAGAGACACGCGCAATTTTACGTGCTGCGGATTAGAACGTCGCAGATCGATGTGCATAGTCTCAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcag                                                                                                                                     <  2:111274/143‑1 (MQ=255)
cACTGATAAGCTGTGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcag                                                                                                                                     <  2:487249/143‑1 (MQ=255)
cACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcag                                                                                                                                     <  1:1118087/143‑1 (MQ=255)
 aCTGTTAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagt                                                                                                                                    <  2:1049852/143‑1 (MQ=255)
 aCTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGCGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagt                                                                                                                                    <  1:728254/143‑1 (MQ=255)
 aCTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagt                                                                                                                                    >  1:309590/1‑143 (MQ=255)
    gacaaGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTGTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgtagtaca                                                                                                                                 <  1:334390/140‑1 (MQ=255)
      tAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATc                                                                                                                               <  1:426374/143‑1 (MQ=255)
        aGCCCGGCTGTAGTGTTTTCCGTAGCGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCa                                                                                                                             >  2:1423922/1‑143 (MQ=255)
         gCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGGGCAGTACATCCAg                                                                                                                            >  2:576095/1‑143 (MQ=255)
          cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGCTACGGGATGGCTGTGCAGGACATACAGt                                                                                                                           >  2:409839/1‑143 (MQ=255)
           gctgCTGTAGTGTTTTCAGGAGAGACACGCGCAATTTTCCCTGCTGCGGATGAGAACGACGAAGCACGATGTGCATAGCCTGAAGTGTACATAATCAATACAGAACCTGTCCCTGACGGTATCGATGTCCAGTACATCCAGTg                                                                                                                          <  2:1037423/140‑1 (MQ=255)
                  tAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGCGCAGTACATCCAGTGCcaacaa                                                                                                                   >  2:981122/1‑143 (MQ=255)
                   aGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGCACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACaaa                                                                                                                  >  2:862019/1‑143 (MQ=255)
                    gtgtTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCCATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAg                                                                                                                 >  1:126110/1‑143 (MQ=255)
                    gtgtTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAg                                                                                                                 >  1:871231/1‑143 (MQ=255)
                         ttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGCAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTGCTGTATGGGTGTGCAGTACATCCAGTGACCACAAAGATCaa                                                                                                            >  1:451427/1‑143 (MQ=255)
                         ttCAGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCaa                                                                                                            <  2:1008371/143‑1 (MQ=255)
                           ccGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAAcc                                                                                                          <  1:416038/143‑1 (MQ=255)
                                   acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                  >  1:1441419/1‑143 (MQ=255)
                                      cgcgCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGa                                                                                               <  1:977641/143‑1 (MQ=255)
                                      cgcgCAATTTGACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGa                                                                                               <  1:44440/143‑1 (MQ=255)
                                       gcgcAATTTTTCTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAATGATCAACCCTATTTTCGGaa                                                                                              <  2:39124/143‑1 (MQ=255)
                                       gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGaa                                                                                              >  1:1417436/1‑143 (MQ=255)
                                       gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGaa                                                                                              >  2:1303042/1‑143 (MQ=255)
                                        cgcAATTTTACTTGCTGCGTATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGaaa                                                                                             >  1:866559/1‑143 (MQ=255)
                                        cgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGaaa                                                                                             <  2:546979/143‑1 (MQ=255)
                                         gcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAAACAATCCAGCACCTGAGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGAACAACCCTATCTTCGGAAag                                                                                            >  2:145571/1‑143 (MQ=255)
                                          cAATTTTACTTGCTGGGGATGAGAACGACGAAGAACGATGTGCAGAGCCTGAAGTGTACATAATCAAGCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAaga                                                                                           <  1:858836/143‑1 (MQ=255)
                                            actttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGc                                                                                         <  2:927842/141‑1 (MQ=255)
                                              tttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCt                                                                                       <  2:309590/143‑1 (MQ=255)
                                               tttcTTGGTGCGTATGAGATCGACGAAGAACGATGTGCATACCCTTAACTGTATTTCATCAATCCATCTCCTGTGAGTTACTTTATGTATTTGCAGTACATCCAGTGTCAATAAATATCATCCCTATTTTCGGAAAGAGCCTc                                                                                      <  2:1281898/140‑1 (MQ=255)
                                                tACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCg                                                                                     <  1:530640/143‑1 (MQ=255)
                                                   ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaa                                                                                  >  1:857250/1‑143 (MQ=255)
                                                       tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATGTTCGGAAAGAGCCTCGCAAAttt                                                                              >  2:1044301/1‑143 (MQ=255)
                                                        gcGGAGGAGAACGACGAAGCACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                             <  1:185089/143‑1 (MQ=255)
                                                               agaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                      <  1:1419356/143‑1 (MQ=255)
                                                               agaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                      <  1:1224217/143‑1 (MQ=255)
                                                                 aaCGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTggg                                                                    >  1:220597/1‑142 (MQ=255)
                                                                 aaCGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGt                                                                    >  1:547830/1‑143 (MQ=255)
                                                                   cgacgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGa                                                                  >  1:368545/1‑143 (MQ=255)
                                                                   cgacgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGa                                                                  >  1:697159/1‑143 (MQ=255)
                                                                      cgcagacCGATGTGCGTAGCCGGAAGTGTACATAATCAATCCAGCCCCTGGGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACgg                                                               <  2:623623/140‑1 (MQ=255)
                                                                      cgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACgg                                                               <  1:1423922/143‑1 (MQ=255)
                                                                              taTGTGCATATCCTGAAGTGTACATAATCAATCCATCCCTGGTGAGTTCCTGTATGGATGTGTAGCACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAACCTCACAAATTTTGTCGTTGGTGACGGGAAAACAt                                                       <  2:286250/142‑1 (MQ=255)
                                                                              gctgtgCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGCATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACAt                                                       <  2:1002095/141‑1 (MQ=255)
                                                                              gATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGCGCCTCGCACTTTTTGTCGTTGTTGAGAGGAAAACAt                                                       >  1:207147/1‑143 (MQ=255)
                                                                               aTGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATa                                                      >  1:1011417/1‑143 (MQ=255)
                                                                                   gCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAAtt                                                  >  1:1334392/1‑143 (MQ=255)
                                                                                    cATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGCATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAACTTTTGTCGTTGGTAACGGGAAAACATAACttc                                                 >  1:531974/1‑142 (MQ=255)
                                                                                         ccTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTGACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCtt                                            >  1:935425/1‑143 (MQ=255)
                                                                                          cTGAAGTGTACATGAGCAAGCCAGCCCCTGTGAGGTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAGCCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTg                                           <  2:44117/143‑1 (MQ=255)
                                                                                          cTGAAGTGTACATAATCAATCCAGCACATGAGTATTCCGCAAGGGAGGTGCAGTACATCCAGTGACAACTAAGATCAACCCTATCCCCGGAAAGAGCCTCGCAAAGGTTGTCGCAGGTTACGGGAAAACATAAATTAATCTTg                                           >  2:876432/1‑143 (MQ=255)
                                                                                           tGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAGACATAAATTAATCTTGc                                          >  1:1317013/1‑143 (MQ=255)
                                                                                           tGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCATATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCtttc                                          >  1:801888/1‑141 (MQ=255)
                                                                                                tgtACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGATAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCtt                                     >  1:1440162/1‑143 (MQ=255)
                                                                                                tgtACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATACATTAATCTAGcccact                                     >  2:14942/1‑140 (MQ=255)
                                                                                                 gtACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAGAACATAAATTAATCTTACCCCTTa                                    >  1:1331776/1‑143 (MQ=255)
                                                                                                  tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTaa                                   <  2:483161/143‑1 (MQ=255)
                                                                                                  tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTaa                                   >  1:583027/1‑143 (MQ=255)
                                                                                                  tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTGTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACACAAATTAAGCTTGCCCCTTaa                                   >  2:34799/1‑143 (MQ=255)
                                                                                                   aCATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAACTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCGTTacg                                  >  2:1227315/1‑141 (MQ=255)
                                                                                                    cATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGGCGGGAAAACATAAATTAATCTTGCCCCTTAGGa                                 >  1:88870/1‑143 (MQ=255)
                                                                                                      tAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTCTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAAt                               >  1:574086/1‑143 (MQ=255)
                                                                                                        atcagtcCAGCCCCTGTGAGTTACTGCATCGATGTGCAGTACATCCAGGGACAACAAAGATCAACCCTATTTCCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATaa                             <  1:409839/143‑1 (MQ=255)
                                                                                                           actcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGtt                          <  1:377275/141‑1 (MQ=255)
                                                                                                           actcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCGCGCAAATGTTGTCGTTGGTGCCGGGAACACATCAATTAATCTTGCCCCTTAAGAATAAGtt                          <  2:880636/141‑1 (MQ=255)
                                                                                                           aatcCAGCCCCTGTGAGTTACTGTATGGATGCGCAGTACATCCAGTGACAACAACGTTCCACCATATTATTTGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGtt                          <  2:1175686/143‑1 (MQ=255)
                                                                                                            atcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTCAGAATAAGTTg                         >  1:763183/1‑143 (MQ=255)
                                                                                                             tcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTCATCTTGCCCCTTAAGAATAAGTTGc                        >  2:519670/1‑143 (MQ=255)
                                                                                                             tcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                        <  2:282100/143‑1 (MQ=255)
                                                                                                             tcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                        >  2:1159316/1‑143 (MQ=255)
                                                                                                             cccAGCCCTTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                        <  2:287794/142‑1 (MQ=255)
                                                                                                                  ccccTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTAtt                   >  1:210018/1‑143 (MQ=255)
                                                                                                                       gtgAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGt              <  1:437430/143‑1 (MQ=255)
                                                                                                                        tgcgTTACTGGATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa             <  2:1394339/140‑1 (MQ=255)
                                                                                                                        tgAGTTACTGGATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCTTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa             <  1:352129/143‑1 (MQ=255)
                                                                                                                          aggtACTGTATGGATGTGCAGTACATCAAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGt           <  1:19164/140‑1 (MQ=255)
                                                                                                                            ttACTGTATGGATGTGCAGTACATCCAGTGGCAACAAAGATCAACCATATTTCCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCGTATTTTCGTAGTTa         >  1:548697/1‑143 (MQ=255)
                                                                                                                            ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa         >  1:708965/1‑143 (MQ=255)
                                                                                                                             ttgtcactGGATGTACTGCACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTaa        >  1:1405935/8‑143 (MQ=255)
                                                                                                                             tACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTaa        <  2:1417436/143‑1 (MQ=255)
                                                                                                                               cTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTCTATCCCGAAAGATCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACg      <  1:1435197/143‑1 (MQ=255)
                                                                                                                                tGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCATATTTTCGGAAAGAGCCTCGCAAATTATGTCGTTGGTGACGGGAAAACATAAATTAATCTTACCCCTTAAGAATAAGTTGCCTCTTTTCAAAGATAAcag     >  2:492900/1‑141 (MQ=255)
                                                                                                                                 gTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGa    <  1:1360056/143‑1 (MQ=255)
                                                                                                                                  tATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt   <  1:290452/143‑1 (MQ=255)
                                                                                                                                   aTGGATGTGCAGTACATCCATTGGCGACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATGTTGGCGTTGGTGACGGGAAAACATAAATTAATCTTGCCGCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATc  <  2:631778/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATC  >  NC_000913/1020817‑1021090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: