Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,065,745 A→G 12.5% E54G (GAG→GGG)  agp → glucose‑1‑phosphatase/inositol phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,065,7450AG12.5% 194.6 / 3.6 76E54G (GAG→GGG) agpglucose‑1‑phosphatase/inositol phosphatase
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base G (9/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGA  >  NC_000913/1065508‑1065982
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
tttGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGGTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGGGCTCATGATGAGCCGCCATAACTTACGCGCGCCGCCGGCGGACAATGGCCGGGGGCTGGAg                                                                                                                                                                                                                                                >  1:258269/1‑239 (MQ=255)
          tGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCCCTGGCGAAAAAAGGCAGGGTGCTGGGGCAGTCGACg                                                                                                                                                                                                                                      >  1:132164/1‑238 (MQ=255)
                      taattcttttttctttcACCCTTTTTTGTGATTAATACAAAAAGGGGGCCCAGAAAACACCAAACCCTAAACGGCCCGAGCTGTGGCAGGGATAGTTTAACTCCTTTCAAACCCACATGCAAAAACCGTACCGGAAGGCTATCAGCTAAGGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGc                                                                                                                                                                                                                         <  2:267868/230‑1 (MQ=255)
                                    gTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCCGGCGGAAAATGGCCGTGGGATGGGGCAGGCGACGCCGAATAAATGGCCAGAATGGGACGt                                                                                                                                                                                                            >  1:510879/1‑238 (MQ=255)
                                             tttAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGgggg                                                                                                                                                                                                   >  1:80436/1‑238 (MQ=255)
                                                   ggttgaaAAAAAAAGGGGTGCCAGGAAGAAAAAAACCGCTAACCGCCGCACGTGGGGCAGGGTAGTTTTACGCCGCGTAAACAGACCAGGCCAAAACCTCACCGAAGGGCAACCGGCACAAGCATGTCCTCAGGAGGGGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTc                                                                                                                                                                                            <  2:246822/237‑1 (MQ=255)
                                                     ttgatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGGGGGCTGGAGCAGTCGCCGCCGAATAAATGGCCCAAATGGGACGGCCCCGGTGGGCacatcc                                                                                                                                                                                           >  1:246822/1‑233 (MQ=255)
                                                     ttgatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCCGGCGAACAATGGCAGTGTGCTGGGGCAGTCGACCCCGAAAAAAAGGCCAAAAAGGGACGGTCCCGGGGGGCaaacccc                                                                                                                                                                                          >  2:918785/1‑234 (MQ=255)
                                                       gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCCGAATGGGa                                                                                                                                                                                                               >  1:119398/1‑216 (MQ=255)
                                                       gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTcacc                                                                                                                                                                                         >  1:9118/1‑238 (MQ=255)
                                                       gatAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTAATCGCTTCAAACGGTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCCTAACTTACGTGCGCCGCCGGCGAACAAAGGCAAGGTGCGGGGGCAGTCGACCCCGAAAAAATGGGCAGAAAGGGGAAGCCCCGGGGGGGAACCcaca                                                                                                                                                                                         >  2:982017/1‑237 (MQ=255)
                                                               aaGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCCCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCCCTGTCGAACAATGGCAGTGGGCTGGGGCAGTCGCAGCCGAGAAAATGGCCAGAATGGGGCGTCCCCGGTGGGGGACCCACCACCaaaaag                                                                                                                                                                                >  2:1038450/1‑236 (MQ=255)
                                                                    ggcccAGGAATGAAAAAAACCATAACCGCCGCAGGTGGGGCAGGGATAGTTTTACCCGCTTCAAACGCTCAGGCAAAAACCGTCCCGGAAGGCTAGCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCg                                                                                                                                                                            <  1:602368/235‑1 (MQ=255)
                                                                    gTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTTTCAGCCACCACAAGTGCTCATGATGAGCCGCCATAACTTACGGGCGCCGCTGGCGAACAATGGCAGTGTGCTGGGGCAGGCCACGCCCAACAAAATGCCCAAATGGGACGGCCCCGGGGGGGAAATCACCCCCCAAAGGGGCGt                                                                                                                                                                           >  2:978658/1‑239 (MQ=255)
                                                                    gTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGGGGGCTGGGGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGGGGGCAACTCACCACCAAAGGGGGCg                                                                                                                                                                            >  1:355334/1‑238 (MQ=255)
                                                                       ccAGAAATAAACAAACCGATAACCCCCGCAGGTGTGGCAGGGATATTTTTACTCGTTTCAAACGCTCGGGTACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCt                                                                                                                                                                        <  1:283744/239‑1 (MQ=255)
                                                                         aGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGCATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCCCCACCAAAGGTGGCGTGCTCg                                                                                                                                                                      >  1:1002912/1‑239 (MQ=255)
                                                                           gAATGAACAAAACGCTACTCGCCGCAGCTGTGGCAGGGGTAGTTTTACTCGCTTCCCACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCAGAAATTACGGACCCCCCCGGCGGACAATGGCGTGGCGCTGGGCACATCGACCCCCAATAAAAGGCCAGCATGGGGGGAGCCCAGGGTGCAACACAACACCCAAGgcggcgacgtagaa                                                                                                                                                                    >  2:239240/1‑231 (MQ=255)
                                                                            aaggAACAAAACCGTAACCGCCGCGTGTGTGGCAGGGATATTTTTCCTCGTCTAAAACGCTAAGGCACAAACCGTCCGGGAAGGCTAACAGCTAACGCAAGGGCTAAGGATGAGCCGCCAGAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                   <  2:80436/235‑1 (MQ=255)
                                                                            aagaaaCAAACCGCTAAGCGCCGCAGCTGGGGCAGGGATAGTTTTACTCGCTTCAAACGCCCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                   <  1:836742/234‑1 (MQ=255)
                                                                            aacaaaCAAACCGCTAACCGCCGCAGTTGTGGCAGGGATATTTTTCCTCGCTCAAACCGCTCAGACACAACCCGAACCGGAAGGCTTTCAGCTACAGAAGGTGCTCAGGAGGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                      <  2:247400/232‑1 (MQ=255)
                                                                            aaagAACAAAACGCTAAACACCTCAGCTGTGGCAGGGAAAGTTTTACTCGCTTCAAACCCCAGGGCAAAAACCGTTCCGGAAGTCTATCAGCTACGGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGaa                                                                                                                                                                   <  1:388736/235‑1 (MQ=255)
                                                                            aaTGAACAAACCCTAAACCCCCGCAGTTGTGGCAGGGATAGTTTTCCCCGCTTCAAACCCTCAGGCAAAAACCGTACCGGAAGGCTATAAGCTAAAGCAAGTGCTAATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                      <  1:982017/236‑1 (MQ=255)
                                                                            aaTGAACAAAACGCTAACCGCCGCAGCTGTGGCAGTGATAGTTTTACGCGGTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGAAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCg                                                                                                                                                                      <  1:872143/236‑1 (MQ=255)
                                                                            aaTGAACAAAACGCTAACCCCCGCGGCTGGGGCAGGGATAGTTTTACTCCCTACAACCGCTCAGGCACAACCCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGCGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGa                                                                                                                                                                     <  1:112799/237‑1 (MQ=255)
                                                                                aaCAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCCGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGta                                                                                                                                                              >  1:960229/1‑239 (MQ=255)
                                                                                 aCAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCCAAAGTGGCGGGCTCGAAGTGgat                                                                                                                                                             >  1:247400/1‑239 (MQ=255)
                                                                                  cAAACCGCTAATCGCCGCAGCTGGGGCAGGGATAGTTTTACTCGCTTCAACGTCCAAGGCACAAACCGTCCCGGAAGGCTACCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGtata                                                                                                                                                            <  1:1038450/239‑1 (MQ=255)
                                                                                     aaCGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGGGTatattg                                                                                                                                                         >  1:652399/1‑237 (MQ=255)
                                                                                            aTCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGGGGCGTGCTCGAAGGGTATATGGGCCCTTa                                                                                                                                                  >  1:422052/1‑239 (MQ=255)
                                                                                                  cgcAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGGGCAGTCGACGCCGAATAAATGGCCAGAACGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGGGCTCGAAGGGGATATTGGGCATTACACGc                                                                                                                                             >  1:552579/1‑239 (MQ=255)
                                                                                                            gCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGGGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGGGAAtg                                                                                                                                      >  1:1035588/1‑236 (MQ=255)
                                                                                                                      ttttACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGGGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAg                                                                                                                         >  1:1016643/1‑239 (MQ=255)
                                                                                                                      ttttACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATAAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGGGCAGTCGACGCCGAATAAATGGCCCAAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGGGGCGGGCTCGAAGGGGATATGGGGAATTACAAGCGGGAATGGCTGGCAgaggag                                                                                                                         >  2:417109/1‑239 (MQ=255)
                                                                                                                           cTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGGGAATGCCTGGCCGAGCAGGGGAt                                                                                                                    >  1:702428/1‑239 (MQ=255)
                                                                                                                                           cAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACCTGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGa                                                                                                     >  1:732873/1‑239 (MQ=255)
                                                                                                                                                    aCCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTggg                                                                                                                                                                                                  >  2:378008/1‑138 (MQ=255)
                                                                                                                                                    aCCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTggg                                                                                                                                                                                                  <  1:378008/138‑1 (MQ=255)
                                                                                                                                                      cGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGGGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccgccg                                                                                           >  1:1072806/1‑238 (MQ=255)
                                                                                                                                                        tACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGTACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGAATCTGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAACCgggg                                                                                                       >  1:75980/1‑224 (MQ=255)
                                                                                                                                                        tACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACg                                                                                                                                                                                                                                      <  1:1042031/98‑1 (MQ=255)
                                                                                                                                                        tACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACg                                                                                                                                                                                                                                      >  2:1042031/1‑98 (MQ=255)
                                                                                                                                                        tACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccgccgccg                                                                                        >  1:919673/1‑239 (MQ=255)
                                                                                                                                                        tACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGAGGGTGAAATCGGGGGAATGCccgccgccg                                                                                        >  1:1084594/1‑239 (MQ=255)
                                                                                                                                                        tACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGGGAATGGCTGGCAGAGCAGGGGATGGGGAAATCGGGGGAATGCccgccgccg                                                                                        >  1:770338/1‑239 (MQ=255)
                                                                                                                                                           gggatggatcgcAGCTACATGAAGCACTCATGGAGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGGGTGCTGGAGCAGGCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCTCCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTac                                                                                     <  1:581815/228‑1 (MQ=255)
                                                                                                                                                                ggCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGGATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGGACACCGt                                                                                >  1:498220/1‑239 (MQ=255)
                                                                                                                                                                    aTCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGAGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGCTGGCGTGCTCGAAGTGTATATGGGCCCTTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAATCGGGGGAATGCCCGCCGCCCTACACCGTTta                                                                             >  1:315552/1‑238 (MQ=255)
                                                                                                                                                                     tCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGGAGTCCCCGGTGGGCAACTCACCACCAAAGGGGCCGTGCCTGAGGGTGTTTTGGGCCATTAAAGCCGGGAATGGCTGGCACAGCAGGGGGAGGGGAAAACGGGGGAATGCCCCGCCCCGGACACCGTTtat                                                                            >  2:545856/1‑238 (MQ=255)
                                                                                                                                                                     tCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGTCCGCCCCCGTAAACCGTTtat                                                                            >  1:523868/1‑238 (MQ=255)
                                                                                                                                                                          tACAGCAAGTTCTAGTGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTtatgcc                                                                         <  1:580403/236‑1 (MQ=255)
                                                                                                                                                                            cAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGGGCTCGAAGTGCATATGGGCCATTACATGCGCGAATGGCTGGCAGGGCAGGGGGTGGGGAAATCGGGGGAAGGGCCGCCGCCGGACACCGTTattgccctgcc                                                                    >  2:261390/1‑234 (MQ=255)
                                                                                                                                                                             aGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTatat                                                                                                                                                           >  1:631462/1‑151 (MQ=255)
                                                                                                                                                                             aGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTatat                                                                                                                                                           <  2:631462/151‑1 (MQ=255)
                                                                                                                                                                                aggTGCTCATGAGGGGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCa                                                                  <  1:164742/235‑1 (MQ=255)
                                                                                                                                                                                     ctaatggtgGGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTc                                                            <  1:978658/235‑1 (MQ=255)
                                                                                                                                                                                        taagggacgggccATAACTTCCGGGCGCCGCTGGGGAACAAGGGCGGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCa                                                        <  2:402259/229‑1 (MQ=255)
                                                                                                                                                                                                           tcggggcgcCGCTGGGGAAAAATGGCAGTGGGCTGGGCCAGTGGACGCCGAATAAAGGGCCAGAATGGGACGTCCAGGGTGGGCACCCCCCCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  2:844225/234‑1 (MQ=255)
                                                                                                                                                                                                           tACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  1:379714/239‑1 (MQ=255)
                                                                                                                                                                                                           tACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  1:526871/239‑1 (MQ=255)
                                                                                                                                                                                                            gcGTGCGCCGTTGGCGAACAGTGGCGGTGGGCTGGAGCAGGCGGCGCCGAAAAAAGGCCCAGAAGGGGACGTCCCCGGTGGGCAACCCACCACCAAAGGTGGCGTGCTGGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGca                                     <  2:498220/237‑1 (MQ=255)
                                                                                                                                                                                                             cGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGtt                                              >  1:402259/1‑228 (MQ=255)
                                                                                                                                                                                                             cGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGGGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGCCTGCAACGTACCGTTGCGGCCGccca                                   >  1:573835/1‑239 (MQ=255)
                                                                                                                                                                                                                 cgccgcTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAAAAGTCTGCAACGAACCGTTGCGACCGCCCAGt                                 >  1:158253/1‑237 (MQ=255)
                                                                                                                                                                                                                 cgccgcTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTAAACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCCCACAGt                                 >  1:922252/1‑237 (MQ=255)
                                                                                                                                                                                                                 cgccgcTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGACAAGGGGATGGTGAAATCGGGGGACTGCCCGCCGCCGTACCCCGCTTATGCCTATGCCAATAGTCCGCAACGCACCGTTGCCACCCCACAAttt                               >  2:696019/1‑238 (MQ=255)
                                                                                                                                                                                                                              cAATGGCAGTGTGTGTGAGCAGTCGAGGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGgcg                  <  1:359181/239‑1 (MQ=255)
                                                                                                                                                                                                                              cAATGGCAGTGTGCTGGGGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGgcg                  <  1:417109/239‑1 (MQ=255)
                                                                                                                                                                                                                                       tgtgCTGGAGCGGTCGACGCCGAAAAAATGGCCAGAATGGGACGCCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAGGTGTAAATGGGCCCTTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCg          <  2:158253/238‑1 (MQ=255)
                                                                                                                                                                                                                                        gtgggggAGCAGGCGCCGCCGAATAAAGGGCCGGAATGGGACGTCCCCGGGGGGCAACTCCCCACCAAAGGGGGCGTGCTCGAAGTGTATATGGGCCATTACAGGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCggg        <  2:592954/234‑1 (MQ=255)
                                                                                                                                                                                                                                        gtgCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccg                                                                                              <  2:415679/153‑1 (MQ=255)
                                                                                                                                                                                                                                        gtgCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCccg                                                                                              >  1:415679/1‑153 (MQ=255)
                                                                                                                                                                                                                                         ggCTGGAGGGGTCGACGCCAAATAAAGGGCCAGAAGGGGACGTCCCCGGGGGGCACACCCCCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTCCATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCgggg       <  2:1035588/238‑1 (MQ=255)
                                                                                                                                                                                                                                         ggCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCgggg       <  1:173940/238‑1 (MQ=255)
                                                                                                                                                                                                                                          gcgggAGCAGGCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCgggg       <  1:696019/235‑1 (MQ=255)
                                                                                                                                                                                                                                           cTGGCGCAGTCCACCCCGAATAAAGGGCCAGAAGGGTGCGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGgtg     <  2:1003608/239‑1 (MQ=255)
                                                                                                                                                                                                                                               aGCAGTCGACGCCGAAGAAATGGGCAGAATGGGGCGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGGGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGa  <  1:517484/238‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
TTTGCGAACCTGTTCCCGGAAAAAAGTCATATTTCTGTCACACTCTTTAGTGATTGATAACAAAAGAGGTGCCAGGAATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCTCAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCATAACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAATGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTGTATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAATGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGA  >  NC_000913/1065508‑1065982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: