Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,175,770 T→C 12.7% L115P (CTC→CCC)  lolC → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,175,7700TC12.7% 146.3 / 3.0 65L115P (CTC→CCC) lolClipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (23/30);  new base C (6/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCC  >  NC_000913/1175532‑1175999
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       
cgcGGGGTTGTGGCGCTTGGCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGATCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCGTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         <  1:438873/237‑1 (MQ=255)
  cGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGTTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCTCg                                                                                                                                                                                                                                       >  1:982328/1‑239 (MQ=255)
   ggggTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCCCgg                                                                                                                                                                                                                                      >  1:675823/1‑239 (MQ=255)
   ggggTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGCTGCTCgg                                                                                                                                                                                                                                      >  1:85296/1‑239 (MQ=255)
   ggggTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCCCgg                                                                                                                                                                                                                                      >  1:870820/1‑239 (MQ=255)
               cTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGACGTTAATCGCGTCGCACCTATTACTACCGGTGATTTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCGCGGTATCGCACCGgc                                                                                                                                                                                                                          >  1:1062246/1‑239 (MQ=255)
                 ggTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCTCGGTATCGACCCGgcgc                                                                                                                                                                                                                        >  1:807962/1‑239 (MQ=255)
                     acaGTATTGTCAGTGAGGAACGGCTTTGAGCGCTAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCaaa                                                                                                                                                                                                                     <  1:855997/238‑1 (MQ=255)
                       aGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGGGATGTGGGACTGCAAAACGGGCGCAGCGTGCGGGTCGGGGGGGTGCGCGGGTTCGGCCCCGCCCAAAAAg                                                                                                                                                                                                                  >  2:830006/1‑239 (MQ=255)
                        ttATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCGGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGa                                                                                                                                                                                                                 <  1:302/238‑1 (MQ=255)
                          aTTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGa                                                                                                                                                                                                                 <  1:291006/237‑1 (MQ=255)
                            tGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAACCGCGCGCAGCGTGGCGGCCGGGGTGATGTTCCGTATCCGCCCGGCGCAAAAAGATccc                                                                                                                                                                                                             >  1:55872/1‑238 (MQ=255)
                            tGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGCCGCACCTATTACTCCCGGTGAGTTGGCACTGCAAAACGCGCCCACCGGGGGCGTTCGGGGGTCTGCCCGGGTGAGACCCGGCGCCAAAAGaac                                                                                                                                                                                                               >  2:765987/1‑236 (MQ=255)
                            tGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGGCAAACTGGACGGCGTTAGTCGCGTCGCACCTATTACAACCGGTGCTGTGGTACTCCAAAACACGCGCGGCGGGGCGGCCAGGGGGGTGTGCGGAGACCACCCGGCGGGAAAAGAACCa                                                                                                                                                                                                             >  2:473144/1‑239 (MQ=255)
                              tCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGGTCGGTATCGACCCGGCGCAAAAAGATCCCCt                                                                                                                                                                                                           >  1:1030843/1‑239 (MQ=255)
                                 gTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGAGCGCCCAGCGTGGCGGTCGGGGGGATGCTCGGTATCGACCCCGCGCACAAAAAACCACTTa                                                                                                                                                                                                         >  1:1006232/1‑238 (MQ=255)
                                 gTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGAGTCGCACCTATTACTACCGGTGATTTGGTACTGCAAAGCGCGCGCAGCGTGGCGGGCGGGGGGAGGCTCGGCGTTGACCCGGCTCAAAAAAATCCACTTac                                                                                                                                                                                                        >  1:551651/1‑239 (MQ=255)
                                   gttgaACGGCTTTGGGCCGCGGCTGCAAAAAAACATCCTTGGCCGGATGCCCAAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCCCGGTATCGACCCGGCGCAAAAAGATCCACTTacac                                                                                                                                                                                                      <  2:673070/237‑1 (MQ=255)
                                   gatgaACGGCTTTGTGCGTGCGTTTGAAAACTAATCCCTTGGCCTGAGGCCACAGGCAAGTCTCTCTTGTGAGCCTGGCTCTCTTAACCCGCAGCAACTCCCAGAGACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCGCCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTacac                                                                                                                                                                                                      <  2:274027/239‑1 (MQ=255)
                                   gatgaACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCCCGGGATCGACCCGGCCCAAAAAGAACCCCTTacc                                                                                                                                                                                                       >  1:673070/1‑237 (MQ=255)
                                    atgaAGGGGTTTGAGCGCGGGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATGCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTacac                                                                                                                                                                                                      <  1:999128/238‑1 (MQ=255)
                                             tttGACCGCGGGCTGCAAATCAACATCCTTGGCCTGATGCCACCGGCAATTCTCTCTTCTGGGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATTGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGt                                                                                                                                                                                            <  2:295846/239‑1 (MQ=255)
                                              ttGAGCCCGGGCTGAAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGt                                                                                                                                                                                            <  1:934409/238‑1 (MQ=255)
                                                              aaaaCAACATCCTTGGCCGGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTTTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGaaacaaac                                                                                                                                                                             <  1:698353/238‑1 (MQ=255)
                                                                        ccTTTGCCTGACGCCACAGGCAATTTCTTCTTCTGAGCATGGCTCCTCTAACCCGCAGCAACCCCCAGAACCGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGc                                                                                                                                                                  <  2:881863/239‑1 (MQ=255)
                                                                        ccTTGCCCTGAGGCCCCAGGCAATTCTCCCGTCGTAGCATGGCTGTCTTAACCCGCAGCAACCCCCAGAAACGGCAGTCAAACTGGACGGGGTTAATCGCGTCGCCCCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGc                                                                                                                                                                  <  2:196129/239‑1 (MQ=255)
                                                                          ttGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCg                                                                                                                                                                <  1:765987/239‑1 (MQ=255)
                                                                             gCCTGCTGCCACCGGCAATTCTCTCTTCTGAGCAGGGCTCCCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCgggg                                                                                                                                                             <  2:249251/239‑1 (MQ=255)
                                                                              ccTGATGCCACAGGCCACTCTCTTGTCTGGGCTGGGCTACTTTACCCCGCGGCAGCTCCCAGAGACGGGAGGCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACGGCCGGTGATGTGGTACTGCAGAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCgggg                                                                                                                                                             <  2:82837/238‑1 (MQ=255)
                                                                                  aTGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCCAAAAGATCCCCCTACACCGTATCTGGTCAATGTGAAACAAAATGACCCCGAGCCGGGGaaaac                                                                                                                                                        >  1:208295/1‑237 (MQ=255)
                                                                                  aTGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCTCGGGATCGGCCCGGCGCCAAAAGATCCACTTACACCCGATCTGGGCAATGTGAAACAAACTGACCTCGAGCCGGGGAAata                                                                                                                                                        >  1:499348/1‑239 (MQ=255)
                                                                                          ggCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         <  1:766603/150‑1 (MQ=255)
                                                                                              aTTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACCCCCAGAAACGGCAGGCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCTACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCt                                                                                                                                            <  1:991729/239‑1 (MQ=255)
                                                                                                           aGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGCCGGGGTGATGGCCGGGATCTACCCGGCCCAAAAAAGACCCCTTAAACCGAAACTGGGCAACTGGGAACAAACCGAACACAAACCGGGGAAAAAAAAAGTCACACTCgcggaaaaaaag                                                                                                                               >  2:493496/1‑228 (MQ=255)
                                                                                                                      ctgaaCCCGCAGGAACTCCCAAGAACTGCAGTCAAACCGGACGGCGTTAAGCGCGTCGCTCCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCt                                                                                                                   <  1:143244/236‑1 (MQ=255)
                                                                                                                               cagcaACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCCCAGCTAGGCGTTaa                                                                                                          >  1:681073/1‑239 (MQ=255)
                                                                                                                               cagcaACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGGGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAAGTTGAAACAAACTGACCTCGAGCCGGGGAAATATAAAGTCATCCTCGCGGAACAACTTGCCTCACAGCCAGGCGGTaa                                                                                                          >  1:412357/1‑239 (MQ=255)
                                                                                                                                    acccccGGAAACGGCAGTTAAACTGGACGGGCTTAAGCCGGGCGCACGTATTACTACCGGTGTTGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACCGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATcgcg                                                                                                     <  2:397431/236‑1 (MQ=255)
                                                                                                                                    acccccAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATcgcg                                                                                                     <  1:754616/236‑1 (MQ=255)
                                                                                                                                    aCTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATcgcg                                                                                                     <  1:493496/239‑1 (MQ=255)
                                                                                                                                                  cAGTCAAACTGGAAGGCGTTTATCGCGTCGGACCTACTACTACCGGTGATGTGGCACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACGCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCGCGAGCCGGGGAAATATAATGTCATCCTCGGTGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCcg                                                                                        <  1:868100/238‑1 (MQ=255)
                                                                                                                                                    atccatcgggAGGGGGTTAATCGCGTCGCCCCTTTTACGACCGGGGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGt                                                                                     <  2:1062246/231‑1 (MQ=255)
                                                                                                                                                      aaaaCTGGACGGCGTTAATCGCGTGGCCCCTATTACTACCGGTGAGGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGa                                                                                  <  2:85296/238‑1 (MQ=255)
                                                                                                                                                              gcGGGGTTAATCGCGTCGCACCTATTTCTACCGGTGATGTGGTACGGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTAcc                                                                           <  1:196772/237‑1 (MQ=255)
                                                                                                                                                                            gTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACt                                                                                                                                                                                                           <  2:956748/98‑1 (MQ=255)
                                                                                                                                                                            gTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCCCGGTATCGACCCGGCGCAAAAAGATCCACt                                                                                                                                                                                                           >  1:956748/1‑98 (MQ=255)
                                                                                                                                                                              ctgtccTTTTCCTACCTGTGATGGGCTCCTCCAAAGCGCGCGCGGTGTGGCGGTGGGGGTGATGCCGGGTATCGACCCGGCGCAAAAAGGTCCCCTTACACCGTATCGGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCa                                                          <  2:499348/235‑1 (MQ=255)
                                                                                                                                                                               gCACCTATTACTGCCGGTGATGGGGTCCTGCAAAGGGGTCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCa                                                          <  1:962448/238‑1 (MQ=255)
                                                                                                                                                                                 cccTATTACGACCGGTGATGTGGTACGGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCcgcg                                                                                      <  1:42803/208‑1 (MQ=255)
                                                                                                                                                                                     aTTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGGTGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTAcc                                                                           <  1:621344/215‑1 (MQ=255)
                                                                                                                                                                                             cGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTa                                           >  1:771278/1‑239 (MQ=255)
                                                                                                                                                                                             cGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCCCGTGATGGTACCATCTGCCAGCCCGTTCACGCCGATGGGGCGTa                                           >  1:681485/1‑239 (MQ=255)
                                                                                                                                                                                               gTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGaaca                                                                                                                                   >  2:143245/1‑151 (MQ=255)
                                                                                                                                                                                               gTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGaaca                                                                                                                                   <  1:143245/151‑1 (MQ=255)
                                                                                                                                                                                                tGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCGTCCTCGGCGAACAACTTGCCTCACAGCTAGGCGGTAATCGCGGTGATCAAATACGCGGGCTGGTACCTTTTGCCAGCCAGTTCACGCCCATTGGGCGGATTc                                        >  1:251619/1‑239 (MQ=255)
                                                                                                                                                                                                 gATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         <  2:454393/47‑1 (MQ=255)
                                                                                                                                                                                                 gATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCt                                                                                                                                                                                                                                         >  1:454393/1‑47 (MQ=255)
                                                                                                                                                                                                  gtggggTACCGCAAAGCGCGCGCCGCGTGGCGGTCGGGTTGATGCCCGGTAACGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTcc                                       <  1:473144/234‑1 (MQ=255)
                                                                                                                                                                                                         aCTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGGGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGGTGATTCCAAGCCAg                                >  1:565393/1‑238 (MQ=255)
                                                                                                                                                                                                             cAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCCCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGGTGGGGCGGATTCCAAGCCAgcg                              >  1:767121/1‑236 (MQ=255)
                                                                                                                                                                                                             cAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCCCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGAAGGTGCGGATTCCAAGCCCGCCCCt                           >  1:1070589/1‑239 (MQ=255)
                                                                                                                                                                                                             cAAAGCGCGCGCAGCGTGGCGGTCGGGGAGATGCTTGGCATCGGCCCCGCGCCAAAAGGACCACTTACCCCGGATCTGGTCAATGGGAAACAAACTGACTCCGAGACGGGGGAAAATAATTGCATCCTCGGGAAAAAACTTGACCACACGCTAGGCGTTCAACGCGGGGGACAAAACCACGTGAGGGGAACACCTGCCAGCCGGATTACCCCGATGGGGGGGATTCCaaacgaaggccc                           >  2:884758/1‑229 (MQ=255)
                                                                                                                                                                                                               aaGCGCGCGCAGCGTGGCGGTCGGGGGGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGGATTCCAAGCCAACGCCTGt                         >  1:818493/1‑239 (MQ=255)
                                                                                                                                                                                                                  cgggcgcAGGGTGGGGGTCGGGGGGATTCTCGGTAGCGCCCCGGCGCAAAAATATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCa                      <  2:681073/236‑1 (MQ=255)
                                                                                                                                                                                                                  cgcgcgcAGCGTGGCGGTCAGGGTGATGCTCGGTATCGACCCGGCGCCAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGGGCCGGGGAAATATTATGTCATCCTCGGCGAAAAAATTTCCCCACAGCTAGGCGTTAATCGCGGGGATCAAATCCCCCTGATGGGACCAACCGCCACCCATTTCACGCCGGTGGGGGGTATTCCAAGCCAACGCCGGGTCa                      >  2:17265/1‑239 (MQ=255)
                                                                                                                                                                                                                    cgcgcAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCCCTTACACCGTATCTGGTCAAAGTGAAACAAACTGACTTCGAGCCGGGGAAATTTAATGTAATCCTGGCCGAACAACTTGCCTCCAAGCTGAGCGTTAATCTGGGAGATCAAAACAGGTGCTTGGTACCACGACCCACCCAGTTTAACCCGATGGGGGGGTTTCCAATCCACGCCCGtttcaaa                   >  2:804818/1‑234 (MQ=255)
                                                                                                                                                                                                                    cgcgcAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCCAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCTCGGTGACCAAATCCCCCGCTTGGTACCCACTCCCCGCCAGTTCACGCCCATAGGGCGGATTCCAAACCAGCGCCCCTTCaaa                    >  2:203381/1‑238 (MQ=255)
                                                                                                                                                                                                                     ccctcggtctGGCGGTCGGGGTTATTCTCGGGATCGCCCCGGCGAAAAAAGCTCCACTTCCCCGGTATCTGGTCAATGTGAAACAAACTGTCCGTGGGCCGGGGAAATATAATGTCAGCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCCGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAAt                    <  2:726759/230‑1 (MQ=255)
                                                                                                                                                                                                                                        ggTGATGCTAGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTACTTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTcgcc  <  1:804818/238‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       
CTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCC  >  NC_000913/1175532‑1175999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: