Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,242,879 T→G 16.1% T68P (ACC→CCC)  ldcA ← murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,242,8790TG16.1% 163.4 / 2.6 74T68P (ACC→CCC) ldcAmurein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
Reads supporting (aligned to +/- strand):  ref base T (23/35);  new base G (13/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.32e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAAT  >  NC_000913/1242645‑1243111
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           
aaTGGTGCTGGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGgtgt                                                                                                                                                                                                                                           <  1:230761/237‑1 (MQ=255)
   ggcgCTCGGTAAAGGCGTTCAGTTCATCCGCGCAAAAGTTTGCTACAAGCATGGGGCCGCTAAAGGTAATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAACCGCTATGTCCGCAAATGAGTAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTgggg                                                                                                                                                                                                                                      <  1:505919/236‑1 (MQ=255)
         cGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCAACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGGACCGGAAACGCCGCGGAACGCCCTGACGATGGGGTTggggggcagc                                                                                                                                                                                                                                >  1:654951/1‑234 (MQ=255)
         cGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACAGGTCATGTTGTTGGCTAGCCACCAGTGCCTTGCAGTCAATATCTGCCCGTAAACGACTGGCACCGTAACACCCCCGTAAAGCAAGCACAATGGGGTGGGGGGGagtc                                                                                                                                                                                                                                >  1:488447/1‑239 (MQ=255)
         aggaaaaGGCGTTCAGTTCCTCCGCGCCAAAGTTGGCTCCCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGCCCGCACGGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTagtc                                                                                                                                                                                                                                <  1:64822/235‑1 (MQ=255)
                                 cgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCCAATGAGCAAAGGGCCATGTTGTTGGCGAGCCCCCCGTGCCTGCCAGTCAATATCTGCCATGTAACGCCCCGCACCGTAACCCCCCCGTACAGCGCTCACCGTGGGGTTGGGGGAAGTACGCCTGGGAAGGGAAttaaaaca                                                                                                                                                                                                        >  2:358033/1‑232 (MQ=255)
                                 cgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGTGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCCCCCTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGGCAGCCTGGCAAGGGACTTGAGACCt                                                                                                                                                                                                       >  1:117145/1‑239 (MQ=255)
                                 cgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCCCGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTTAGATCt                                                                                                                                                                                                       >  1:1100526/1‑239 (MQ=255)
                                 cgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGGAGGCAGTCTGGCAAGGGAATTGAGATCt                                                                                                                                                                                                       >  1:875736/1‑239 (MQ=255)
                                         tgggCTACCAGCTGGGGGCGGCTAGAGGGTATGCGATTGCCGTGTGCAAGAAGACCGCATTGAATGGCGTTAAAGCCGGTTGGACCGGAAAGGAGCAACGGGTCTGGTTTTTGGGGGGCCCCCAGTGCCTGCCAGTAAATATCGGCCAGTAAACGGGTGGCACCTTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGa                                                                                                                                                                                                <  2:41820/234‑1 (MQ=255)
                                          ttGCTACCAGGATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTAGGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACg                                                                                                                                                                                              <  1:690327/238‑1 (MQ=255)
                                                   gCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGGAGTCAGTCTGGCAAGGGAATTGAGATCTTCCCGACGCCCTGTTTcc                                                                                                                                                                                    >  1:9960/1‑239 (MQ=255)
                                                              cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCCCTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCaaa                                                                                                                                                                         <  1:768117/239‑1 (MQ=255)
                                                              cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGGGCCTGCCCGTCAATATCGGCCAGTAAACGCCTGGCACCGTAACCGCCGCGTACAGCCCGCACGATGGGGTTGGGGGGAGCCAGTCTGCCCAGGGACTTTAGCACTTCCACACCCCCTTTTTCCGAACCCCCaaa                                                                                                                                                                         >  2:892713/1‑239 (MQ=255)
                                                              cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAAACGCTCTGTCCGCAAATGAGCAACCGGTCATGTCGTTGTCGCGCCACCACTGCCCCCCAGTAAAATTCTGCCAGTAAACGAACGGGACCGTAACCGCCGGCTACACGCCGCCCGCTCGGCTCGGGGGTGGTCAGCGCTGGCCGGGGACATGGATCTTCCAAACCCCCTGTGTCCGCAcaccccaa                                                                                                                                                                         >  2:993868/1‑232 (MQ=255)
                                                                       atgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGCTGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATATTCCAGACGCCCTGTTTCCGTACCCGCAAAACGCTcaca                                                                                                                                                                >  1:202443/1‑239 (MQ=255)
                                                                       atgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCCCTGTTTCCGTACCCGCAAAACGCTcaca                                                                                                                                                                >  1:815849/1‑239 (MQ=255)
                                                                       atgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGGAGTCAGTCTGGCAAGGGAATTGAGACCTTCCAGACGCTCTGGTTCCGTACCCGCAAAACGCTcaca                                                                                                                                                                >  1:598439/1‑239 (MQ=255)
                                                                        tgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGGAGCCAGTCTGGCAAGGGAATTGAGACCTTCCAAACCCCCTGTTTCCGTACCCCCAAACCGCTCCCaa                                                                                                                                                               >  1:139050/1‑239 (MQ=255)
                                                                           aaTTGCCAGGCGCCGAAAGCCCGCACTGAAGGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCGGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAacga                                                                                                                                                            <  1:144861/238‑1 (MQ=255)
                                                                            tttcccttGCGCCAGAAGCCCGCATTGAAGGGCGGTAAAATCGCTATGTCCGAAAATGAGCAACGGGTCATGTTGTGGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAACCGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAacgac                                                                                                                                                           <  2:875736/232‑1 (MQ=255)
                                                                                   tGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGGATTGAGATCTTCCAGACGCCCTGTTTCCGTACCCGCAAAACGTTCACCAAGCCGGGGAAt                                                                                                                                                    >  1:392612/1‑239 (MQ=255)
                                                                                     cgcCAGAAGACCGCACTGAATGGCGGTAAAATCGCCTTGTCCGCAAATGAGCAACGGGTCCTGTTGTTGGAGAGCCACCAATGCCTGCCAGTCAAAATCTGCCAGGAAACGACAGGCCACGTAACCGCCCCGATACGCCAGACAGACGGGCGTGGGGGGAGGAAAGCCTGACAGGGAAAGGAGATTGTCCAGAAGGCCTGTTTCCGGACCCCCAAAACGCTCAAAAAAACGGGaaaaaa                                                                                                                                                  >  2:399875/1‑239 (MQ=255)
                                                                                      gcCAGAAGACCGCACTGAATGGCGGAAAAACCGCTATGGCCGCAAATGCGCACCGGGTCATGTTGTGGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACg                                                                                                                                                                      <  1:500663/218‑1 (MQ=255)
                                                                                          gaagaCCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCCGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCCCCAAACGCTCACAACGCCGGGCAAAAAcccc                                                                                                                                              >  1:704633/1‑236 (MQ=255)
                                                                                           aacgccccACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGGGCAACGGGTCATGTTGTTGGCGGGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGa                                                                                                                                            <  1:64510/232‑1 (MQ=255)
                                                                                            aggccggtcgaaaTGGGGGTAAAATCGCGTGGTCCGAAAATGGGCAGGGGGTCATTTTGTGGGCGAGCCCCCGGTGCCTGCCAGGAAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGa                                                                                                                                            <  2:564687/227‑1 (MQ=255)
                                                                                                cGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGTACCTCCAGCACCCTTGTTTCCAGCCCCCCAAAACGACCACAACGATGGGCAAAAAcaccaacggta                                                                                                                                       >  2:219100/1‑229 (MQ=255)
                                                                                                  cACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCCGCCAGTCAATATCTGCCAGTAAACGACTCGCACCCTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGGAGTCAGTCTGGCCAGGGAATTGAGATCTTCCCGACGCTCTGTTTCCGTACCCGCAAAACACCCCCAACCACGGGCAAAAACACCGACGCTAtt                                                                                                                                     >  2:123406/1‑239 (MQ=255)
                                                                                                  cACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGGTGTTGGCGAGCCACCAGTGCCTGCCAGTCAAAACCTGCCAGTAAAAGACTGGCACCCTAACCGCCCCGGACACCCAGCACGATGGGGGTGGGGGTAGTCAGTCTGGCCAGGGGATTGAGATCTTCCAGCCGCCCTGGTTCCGTACCCCCCAAAAGTCCACAAACACCGGCAAAAAACCCCCCGTTAt                                                                                                                                      >  2:64510/1‑238 (MQ=255)
                                                                                                   ccTGAATGGCGGTAAAATCGCTAGGTCCGAAAAGGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACGGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTg                                                                                                                                    <  1:612151/238‑1 (MQ=255)
                                                                                                             ggTAAAATCGCTATGGCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGGCAATATCTGCCAGGAAACGACTGGGACCGTAACCGCCCCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAAGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCCCAAAACGCTCCCAACGACGGGCAATAACCTCCACGTTATTGGCTTgtagc                                                                                                                           >  1:323639/1‑234 (MQ=255)
                                                                                                                aaatgcGCTATGTCCGCAAATGGGCAACGGGTCATGTTGTTGGCGAGCCCCCCGTGCCTGCCAGTCGTTTACTGGCAGATATTGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCcg                                                                                                                       <  1:782277/234‑1 (MQ=255)
                                                                                                                 aaaTCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCCTACAACCAGCACGATGGTGTTGGGGGGAGTCCGTCTGGCCAGGGAATTGAGATCTTTCCGACGCCCTGTTTCCGGACCCGCAAAAAGCTCCCCACAACGGGGAAAAAACTACCCGTTTTTTTCTTGATAcccccg                                                                                                                      >  2:469458/1‑237 (MQ=255)
                                                                                                                        tATGCCCGAAAATGGACAACGGTTAATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGGCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTa                                                                                                               <  1:892713/239‑1 (MQ=255)
                                                                                                                          tGTCCGAAAAGGGGCAATGGGTAATGTTGTTGGCGAGCCCCCAGTGCCTGCACGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAg                                                                                                             <  1:232129/239‑1 (MQ=255)
                                                                                                                          agccccaaaaTGAGCAACGGGTAATGTTGTTGGCGAGCCACCAGGGCCTGCCAGTCAATTTCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAg                                                                                                             <  1:577491/232‑1 (MQ=255)
                                                                                                                              cGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCACAACGCTCCCAACGACGGGCAATAACCTCGACGTTATTGCCTTGATTCCCCGCGCCGGTAAGgcgc                                                                                                         >  1:205353/1‑239 (MQ=255)
                                                                                                                               gaaaaTGAGCAACGGGCTATGTTGTGGGCGAGCCCCCGGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCt                                                                                                        <  1:833778/237‑1 (MQ=255)
                                                                                                                               gCAAATGGGCAAGGGGTCATGTTTTTGGCAAGCCACCATGGCCTGCCAGTAAATTTCTGCCAGTAAACGACTGCAACCGTACCCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCt                                                                                                        <  1:1069477/239‑1 (MQ=255)
                                                                                                                                      aGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCACGGGAATTGAGATCTTCCAGCCGCTCTGTTTCCGTACCCGCCAAACGCTCACAACGACGGGCAATAACCTAGACGTTTTTGACTTGATGCCCCGCTGCGGTAAGGGGCTGGaaccc                                                                                                 >  2:556420/1‑235 (MQ=255)
                                                                                                                                             ggTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCAAGACGCTCTGTTTCCGAACCCGCAAAACGCTCACAACAACGGGCAATAACCTCTACGTTATTGACTTGAGGCCCCCCTCCGGGAAGGCGCGCGATACCACACAgc                                                                                          >  2:166526/1‑239 (MQ=255)
                                                                                                                                                cgtttttttgGCGGCCCCCCGGTGCCTGCCAGTCAACATCGGCCATGAAACGCGTGGCACCGTACCCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGcgcg                                                                                       <  2:815849/232‑1 (MQ=255)
                                                                                                                                                cATTTTGTTGGCGGACCCCCAGGGCCGGCCAGTCAAATTCTGCCAGGAAACGACGGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGcgcg                                                                                       <  1:990547/239‑1 (MQ=255)
                                                                                                                                                cATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGcgcg                                                                                       >  1:837367/1‑239 (MQ=255)
                                                                                                                                                   gtttttGGCAGGCCACCAGGGCCTGGCAGTCAATGTCGGCCAGTAAACGACTGGCCCCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCgcggcg                                                                                    <  1:147594/239‑1 (MQ=255)
                                                                                                                                                     tgtggGGGAGCCCCCAGGGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg                                                                                  <  1:725319/239‑1 (MQ=255)
                                                                                                                                                     tgtggGCGAGCCACCGGTGCCTGCAAGACAATATCCGGCGGTAAACGTCTGTCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg                                                                                  <  1:358033/239‑1 (MQ=255)
                                                                                                                                                       ttgGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCCAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGt                                                                                   >  1:558417/1‑236 (MQ=255)
                                                                                                                                                             aGCCCCCAGTGCCTGCCAGTCAATATCGGCCAGTAGACGACTGGCTCCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTa                                                                           <  1:185196/238‑1 (MQ=255)
                                                                                                                                                             aGCCCCCAGTGCCTGCCAGTAAATTCCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTa                                                                           <  1:369978/238‑1 (MQ=255)
                                                                                                                                                                  cagtggcctgccAGTAAATTTCTGCCAGTAAACGACTGGAACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAg                                                                     <  1:741328/234‑1 (MQ=255)
                                                                                                                                                                          tgcaAGTCAATATCGGCCAGTAAACGGCTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTa                                                                           <  1:192038/225‑1 (MQ=255)
                                                                                                                                                                                tCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTACGGCGCTGGAAACCACGCAGCGCGGCGTGCTGTTTAATGCAGAAACCCGATGGGGc                                                       >  1:472372/1‑239 (MQ=255)
                                                                                                                                                                                tCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGGGCTGTTTAATGCAGTAACCCGCTGGGGc                                                       >  1:442199/1‑239 (MQ=255)
                                                                                                                                                                                    tatCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGTAGGGAAATAACCTCGACGTTATTGCCTTGATGCCCCGCGACGGGAAGGCGCTGGAAACCACGCAGCGCGGCGGGCTGGTTAATGCAGAAACCCgtgcctataaaa                                                   >  2:192038/1‑228 (MQ=255)
                                                                                                                                                                                        tGCCAGTAAACGCCTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTTTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGt                                               <  1:15223/239‑1 (MQ=255)
                                                                                                                                                                                         gCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGTTACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTg                                              >  1:935052/1‑239 (MQ=255)
                                                                                                                                                                                          ccAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGa                                             >  1:159335/1‑239 (MQ=255)
                                                                                                                                                                                          ccAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGGACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAAc                                                                 >  1:110044/1‑219 (MQ=255)
                                                                                                                                                                                                     cTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGAAACCACGCAGCGCGGCGGGCTGTTTAATg                                                                        >  1:645733/1‑201 (MQ=255)
                                                                                                                                                                                                            ggtgccggccgcGTGCAGCCAGCACGATGGGTTGGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTGTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAAt                           <  2:159335/231‑1 (MQ=255)
                                                                                                                                                                                                            cGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGCTGGGGCAATTAAGTGAAACAGAGACATGGCAAt                           >  1:632143/1‑238 (MQ=255)
                                                                                                                                                                                                              tAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGTCGCTGGATACCCCGCACCGCGGGGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGGGAAACAAGAAAATGGCAAtt                          >  2:593198/1‑237 (MQ=255)
                                                                                                                                                                                                                            cagccaggAGCATGGTGTTGGGGGTAGTCAGGCTGGGAAGGGAATTGAATACTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCGCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAg           <  1:570281/239‑1 (MQ=255)
                                                                                                                                                                                                                               ccagcACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTCCCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg                                                                                  >  2:414786/1‑166 (MQ=255)
                                                                                                                                                                                                                               ccagcACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg                                                                                  <  1:414786/166‑1 (MQ=255)
                                                                                                                                                                                                                                    cACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGCCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCGCATGGGGCAATTAAGGGAACAGCAGACCTGGCAATTCCTTGCTGacaaacaaaaggaa    >  2:137353/1‑229 (MQ=255)
                                                                                                                                                                                                                                     ccggttgtgtTGGGGGGTGTCAGTTCGGCAAGGGAATTGAGATTTTCCAGAAGCTCTGTTTCCGTACCCGCAAAACGCGCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGaa    <  2:1100526/232‑1 (MQ=255)
                                                                                                                                                                                                                                     ccGATGGTGTTGGGGGTAGTCAGTCTGGAAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACg      <  1:399875/235‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGGTGGTTTGGGGGGTACTCAGTCTGGCAAGGGAATTGATATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGGCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACg      <  1:993868/236‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGTTTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACg      <  1:560713/236‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACg      <  1:469458/236‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGTGGTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGaa    <  1:556420/238‑1 (MQ=255)
                                                                                                                                                                                                                                     aCGATGGGGTTGGGGGTAGTCAGTCTGGAAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCGCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGACCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACg      <  1:976846/236‑1 (MQ=255)
                                                                                                                                                                                                                                      cGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATGGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAAt  <  1:542112/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           
AATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCAGTAAACGACTGGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAAT  >  NC_000913/1242645‑1243111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: