Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,825,164	A→T	27.0%	G154G (GGT→GGA)	spy ←	periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,164	0	A	T	27.0%	116.0 / 10.6	69	G154G (GGT→GGA)	spy	periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base A (28/20);  new base T (13/7);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCA  >  NC_000913/1824933‑1825399                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTG‑TT‑CAGTTTTTTTGTCTAA‑AAAATATTCAACA‑GTTGCAGGACCTTTAACCttt                                                                                                                                                                                                                                            >  1:459776/1‑238 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCC‑TT‑AAGTTTTTTGGCTGTA‑AAATTATTTAACA‑GTTTCCGG‑CATTTTTCCtttt                                                                                                                                                                                                                                           >  1:1073144/1‑238 (MQ=255)          gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGC‑TT‑AAGGTTTTTGGCTGAA‑AAACTATTCTGCA‑AGTGCCGG‑CACGTTTCCTTTTTCAGCTGGaa                                                                                                                                                                                                                                 >  1:870877/1‑238 (MQ=255)          gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGGTGTA‑AGATTATTTAGCA‑GTTGCAGG‑CATTTTTCCTTTTTCCGCTggcg                                                                                                                                                                                                                                 >  1:417443/1‑237 (MQ=255)                 gCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGC‑TT‑TAATTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTTTCGCTGGGCGt                                                                                                                                                                                                                               >  1:258688/1‑234 (MQ=255)                      ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGC‑TT‑CAGTTTTTTTGCTGAG‑AAAATATTCAACA‑GATGCGGG‑GACTTTCACTTTTGCAGCCGGGCGGTCtttgctac                                                                                                                                                                                                                     >  2:869339/1‑232 (MQ=255)                                   tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGAACAAGACCGGCGGGC‑TT‑AAACTTTTTTGCTGAACAGTTTATTCAG‑A‑ATTGCAGC‑GACTTTTCCTTTTGCCGCTGGAAGTTTTTT‑CAAACGCCTCTCAAAAtt                                                                                                                                                                                                       >  2:591102/1‑239 (MQ=255)                                                       aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑TAGGTTTTTTGCTTGA‑AGATTATTTACCC‑GGTGTAGG‑CATTTTTCCTTTTTACGCCGGTCGTGTTTT‑TAGACGCTTCTCAAAATATTCATTAAAATGAttttttt                                                                                                                                                                                   >  2:349983/1‑237 (MQ=255)                                                              tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAGGACCGGCGGTCTTT‑AATTTTTTTGATTGAA‑A‑ATTATTTAGCA‑GTTGCAGG‑CATTTTTCCCTTTTCCGCCGGACGTTTTTT‑CTGACGCGTTCCAAAAATAGAATTAAATTATTTTTTTTTTTTCgg                                                                                                                                                                            >  2:565345/1‑239 (MQ=255)                                                                   aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAACA‑GTTGCCAG‑CCTTTTTCCTTTTGCCGCTGGACGTTTTGTCCAGA‑GCTTCTCAAAATTAACATTAAATTGCTTTTTTTTTTCCGGCGT‑Ca                                                                                                                                                                      >  1:411123/1‑239 (MQ=255)                                                                                       gtggtgATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑AT‑AAGGTTTTTGGCTGGA‑AGATTATTTAGCA‑CTTGCGGG‑CCACTTACCACTTGCCCCGGGACGATTCAT‑GAGACCCGTCCCTAATTTTCCATTTAATTATCTTTTTTGTTCCCGCCG‑CCGGATGTTTTAAAAttttttt                                                                                                                                                  >  2:78995/1‑238 (MQ=255)                                                                                            gATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTTAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGGCGTTCTGT‑AAGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCCGCGT‑CAGGATGTTGGTAATTTTGTTCTTGGt                                                                                                                                             >  1:781501/1‑239 (MQ=255)                                                                                             aTCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCTCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GCTGCAGG‑GCTTTTACCTTTTGCCGCTGGACGTTCTGG‑CCGACCCTTCCCAAAATTCACATCTAATTGACTTTTTTGTTCCGGCGG‑CCGGGTGTTGTAAATtttttttgtggtt                                                                                                                                            >  1:627361/1‑232 (MQ=255)                                                                                               cAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTTAGCA‑GTTGCAGG‑CATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGT‑CCGGATGTTGTAAATTTTTTTTTGGGgtt                                                                                                                                           >  1:961401/1‑238 (MQ=255)                                                                                               cAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGGTTTTTTGCTGGA‑AGATTATTCAGAA‑GTTTCAGG‑CATTTTACCTTTTTCCGCTGGACGTTCTGT‑TAGACACTTCTCCAAATTATCATTATATTGCGTTTTTTGTTCCGGCGTCCAGGA‑GGTGTAAAATTTTTTCTTGGgtt                                                                                                                                           >  2:57033/1‑238 (MQ=255)                                                                                                  gCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACAGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑CAGTTTTTTTGCTTAA‑AGATTTCTTCGCA‑GTTGCAGG‑GCTTTTACCTTTTTCCCCTGGAGGGTCTTT‑CCTACGCTTCTCAAAATATCCTTTTAATATCCTTTTCTCTTCTCGCGT‑CAGGCTGTTGTAAATTTTTTTTTGGGtttgcct                                                                                                                                       >  2:5770/1‑235 (MQ=255)                                                                                                    tttttttAGATTTG‑TTGAGAAAGAAAAAAAAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGGCCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑tgt                                                                                                                                    <  1:284529/239‑1 (MQ=255)                                                                                                     ttttttACAACTGTTTGAG‑AAGAGAAGAGAAAA‑CCGCCGCTCCTGTACACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGC‑TT‑ATGTGTTTTTGCTGGA‑GAATTTTTTCGCATGTTAGGGG‑C‑TTTTAACTTTTTCAGATGGAACTTCTCT‑CAGACGCCTCTCCAACTTACCGTTTAGTTACCTTTTTTCTTCGCGCGT‑CAGTGTAATTTCAATTTTGTTCTGGGTTTctagtt                                                                                                                                     >  2:67682/1‑233 (MQ=255)                                                                                                     ttttttACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCC‑TT‑AAGGTTTTTTGCGGAA‑AAATTTTTTAGCA‑GTTTCAGG‑GATTTTTCCTTTTGTCACTGGACGTTCTTT‑CCGAAGGCTCTCAAAATTAGCACTTTATTTTTTTTTTTTTTTCGGTGC‑CAGGGTGTTTTAAAATTTTTTTTGGGGTTccctttt                                                                                                                                    >  2:265947/1‑234 (MQ=255)                                                                                                       ttttACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGCCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGGAGG‑CCATTTACCTTTTGCCGCTGGACGTTCTGT‑CAGAGCCTTCTCAAAATTAGCAGTAAATTTCTTTTTCTGTTCCGGCGG‑CAGGATGTTGGAAATTTTTTTCTTGGGTTCCA‑TTTgc                                                                                                                                  >  1:1039613/1‑238 (MQ=255)                                                                                                        ttcaCAATTGTTGGA‑‑AAGAAAAAAAAAAACCCGCCGATCCTGTCCTCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTgcgc                                                                                                                                <  2:1073144/236‑1 (MQ=255)                                                                                                                 gTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACATTTTGCCGCTGGACGTTTTGT‑CAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTGTTCCGGCGT‑CAGGGTGTTGTTAATTTTGTTTTTGGGTTCCA‑TTTTCGCCAGctggtt                                                                                                                       >  1:1105200/1‑234 (MQ=255)                                                                                                                  tttGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTATCATTAAATTGCTTTTTCTGTTCCCGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGGTTCCC‑TTTGCGCCAGCATTTTa                                                                                                                      >  1:686041/1‑239 (MQ=255)                                                                                                                  tttGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGGTTTTTTGGTGAA‑AGATTATTCAGCA‑GTTTCAGG‑GATTTTTCCTTTTTCCGCTGGACGTTCTGT‑CAAACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTTTGGGTTTTCC‑TCTTCGCCCGCATGTTa                                                                                                                      >  1:115633/1‑239 (MQ=255)                                                                                                                    tGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCGGCGGTC‑TT‑AAGGTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTTTGT‑CAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTTTGTTCCGGCGT‑CATGTTGTTGTAAATTTTTTTCTGGGGTTCCA‑TGTGCGCCAGCATGTTa                                                                                                                      >  1:180373/1‑237 (MQ=255)                                                                                                                             aaaaaaaaac‑CCGCCGATCCTGTCCACCGCATTACGGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTgcg                                                                                                             <  1:591102/236‑1 (MQ=255)                                                                                                                                              aTCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAAACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTTTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTTCGCTGTTCTTTCCttttttc                                                                                           >  1:550758/1‑237 (MQ=255)                                                                                                                                               tCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGcc                                                                                                                               <  1:1022347/203‑1 (MQ=255)                                                                                                                                                        ccggtttACTTAAAGGTGGGGCACAAGCCCGGCGGTT‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAATATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCt                                                                                 <  2:550758/234‑1 (MQ=255)                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TTAAAGTTTTTTGG‑TGAA‑AGAGTATTTAGCA‑ATTTTCAG‑CAGTTTTCCTTTTTCCTCTGGGAGTTCTTT‑CAAACGCCTCTCAAAAATAACATTAAAATCATTTTTTTTGTCCGCGGT‑CAGGAATTTTGCAATTTTGTTTTGGGTTTCCACTTTCCGCCCGC‑TTTTAGA‑TTTTCACTTTTTTTCCATTTTTTCGCTATgcgcc                                                                                 >  2:964428/1‑237 (MQ=255)                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTTGCTTAA‑AGATTTTTCAGCA‑ATTTCAGG‑CATTTTTCCTTTTTCCGTTGGACGTTCTTT‑CAGACGCTTCTCAAAATTAGCATTAAATTTCCTTTTCTTTTCCGGCGT‑CAGGTGGTTGTAAATTTTGTTCTGTTGTTCCA‑TGTGCCCCGCCATGTTAAC‑CTTATGCTGTTCTCCCTTTTTTGCgtacttgcct                                                                                 >  1:557899/1‑229 (MQ=255)                                                                                                                                                         cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGGTTTTTGGCTGAA‑AGATTATTCAGCA‑GGTGCAGG‑CATTTTAACTTTTTTCGCTGGACGTTTTGT‑CAGACGCTTCTTCACAATAGCATTAAATTTCTTTTTTTGTTCCGGCGT‑CAGGATGTTTTAAATTTTGTTCTGGGTTTCCC‑TGTTGGCCAGCCTGTTAGCTTTTTGGCTG‑TCTTCCCTTTTTGCGATCTGCCCt                                                                                 >  1:502680/1‑238 (MQ=255)                                                                                                                                                          gCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                                                               <  1:837034/239‑1 (MQ=255)                                                                                                                                                                 tGCAAGGTAGTGGACAAGCCCGGCGGTC‑TT‑AAGTTGTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAg                                                                                                                             <  1:782513/187‑1 (MQ=255)                                                                                                                                                                   cAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGcc                                                                                                                               <  1:5937/183‑1 (MQ=255)                                                                                                                                                                    aaGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCGGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                     <  1:587453/239‑1 (MQ=255)                                                                                                                                                                      ggTAGTGGACAAGACCGGCGGTC‑TT‑AAGGTTTTTTGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑GATTTTACCTTTTTCCGCTGGACGTTTTGT‑CAGACGCGTCTCAAAATTTGCATTAAATTGCTTTTTTTGTTTCGGCGT‑CAGGATGTTGTTAATATTTTTTTTGGTTTTCC‑TTTGCGGCAGCATGTTTGA‑TTTGTGCTGTTTTTTCATTTTTTTGATTTGCGCTTTAGCTTTTACttt                                                                   >  2:445767/1‑239 (MQ=255)                                                                                                                                                                            ggACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCg                                                               <  1:386437/237‑1 (MQ=255)                                                                                                                                                                                aaGACCGGCGGTC‑TT‑AAGTTTTTTTGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTTACTTTTGCCGCTGGACGTTCTTT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTTCCGGCGT‑CCGGCTGTTGTAAATTTTGTTCTTGGTTTCCA‑TTTGCGCCAGCATGTTAGC‑TTTTCGCTGTTTTTCCCTTTTTTGGTTCTGGGCTTCACCTTTTACTTTTTCGAAGGTa                                                         >  1:863742/1‑239 (MQ=255)                                                                                                                                                                                aaGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCC‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAAt                                                                                                                                                                                                        >  1:1059277/1‑99 (MQ=255)                                                                                                                                                                                aaGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAAt                                                                                                                                                                                                        <  2:1059277/99‑1 (MQ=255)                                                                                                                                                                                  gACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGG‑CCGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATTTTCGCTTCAGCTTTTACTTTTTCTAAGGTAt                                                        >  1:1099406/1‑238 (MQ=255)                                                                                                                                                                                  gACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAACATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTTTGGGTTTCCA‑TTGGCGCCAGCATGTTAGC‑TTTTCGCTGTTCTTCCATTTTTGCGGTCTTCGCTTCAGCTTTTACTTTATCGAAGGTAt                                                        >  1:543887/1‑238 (MQ=255)                                                                                                                                                                                  gACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GGTGCAGG‑CATTTTTACTTTTTCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGG‑CCGGTTGTTGGAAATTTTTGTTTTGGTTTCCC‑TGTGGGCCAGCCAGGTAGA‑TTTTCGCTTTTTTTTCATTTTTTCGATCTGCGCTTTAGCTTTTATTTTATCGAAggggta                                                       >  2:370180/1‑235 (MQ=255)                                                                                                                                                                                     cggcggTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                    <  1:565345/239‑1 (MQ=255)                                                                                                                                                                                         gggtttttattttttttGGCTTAA‑AGATTATTCAACA‑GTTGCAGG‑CATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCCGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTgg                                                  <  2:615616/228‑1 (MQ=255)                                                                                                                                                                                           tC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                               <  1:345574/238‑1 (MQ=255)                                                                                                                                                                                           tC‑TT‑AAGTTTTTTGGCTGAA‑AGATTAATCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                               <  1:850006/238‑1 (MQ=255)                                                                                                                                                                                            c‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  1:142646/239‑1 (MQ=255)                                                                                                                                                                                            c‑TT‑AAGTTGTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  1:349983/239‑1 (MQ=255)                                                                                                                                                                                             ttt‑AAGTTTTTTTGGTTAA‑AGATTATTCAGCA‑GTTGTATG‑CATTTTTCCTTTTGCCGGTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:180373/238‑1 (MQ=255)                                                                                                                                                                                             ttt‑AAGTTTTTTGGCTGAA‑AGTTTATTCAGCA‑GTTGCAGG‑CATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:543887/238‑1 (MQ=255)                                                                                                                                                                                             ttt‑AAGTTTTTTGGCTGAA‑AGATTTTTCAGCA‑GTTGCAGG‑CATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:258688/238‑1 (MQ=255)                                                                                                                                                                                              tt‑AAGTTTTTTGGCTGAA‑AGTTTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGGCGCTGGTCGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  1:727850/238‑1 (MQ=255)                                                                                                                                                                                              ctttatttttttGGGTTAA‑AGATTTTTCAGCC‑GTTGCAGG‑CATTTTTCCTTTTGCCGTTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                             <  2:1099406/233‑1 (MQ=255)                                                                                                                                                                                                       tttGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TTTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGGATCGCTGGCAATGATGTCTTGc                                    >  1:431016/1‑239 (MQ=255)                                                                                                                                                                                                              gAA‑AGTTTTTTCAGTA‑GTTTGAGG‑CATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                             <  2:627361/239‑1 (MQ=255)                                                                                                                                                                                                                    attattCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGt                        <  1:1085902/239‑1 (MQ=255)                                                                                                                                                                                                                           agca‑gTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCa                 >  1:710458/1‑239 (MQ=255)                                                                                                                                                                                                                                 ttGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTTTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                <  2:271669/171‑1 (MQ=255)                                                                                                                                                                                                                                 ttGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                >  1:271669/1‑171 (MQ=255)                                                                                                                                                                                                                                 ttGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggc            >  1:734860/1‑239 (MQ=255)                                                                                                                                                                                                                                 ttGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGAGATCTGAGCTTCAGCTTTTACTTTATCGAAGGCATCGCTGGCAATGATGTCATGCATTTCGCGGCGTTCTTCCatccgc            >  1:297651/1‑234 (MQ=255)                                                                                                                                                                                                                                  tGCTGG‑CTTTTTTCCTTTTGCCGCTGGACTTTCTTT‑CAGACGCTTTTCAAAATTGACATTAAATTTCTTTTTCTGTTCCGGCGT‑CAGGATTTTTTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCTCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg           <  2:781501/239‑1 (MQ=255)                                                                                                                                                                                                                                   gCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTgcg                                                                                                             >  1:814207/1‑140 (MQ=255)                                                                                                                                                                                                                                   gCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTgcg                                                                                                             <  2:814207/140‑1 (MQ=255)                                                                                                                                                                                                                                   gCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGcagcgg          >  1:928058/1‑239 (MQ=255)                                                                                                                                                                                                                                          aTTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCATCGCCGGACGttt    >  1:497480/1‑239 (MQ=255)                                                                                                                                                                                                                                            tttACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCACTGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCa  >  1:1058297/1‑239 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                TCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTC‑TT‑AAGTTTTTTGGCTGAA‑AGATTATTCAGCA‑GTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCA  >  NC_000913/1824933‑1825399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑