Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,120,818 A→G 21.1% L246P (CTC→CCC)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,120,8180AG21.1% 109.3 / 6.0 53L246P (CTC→CCC) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (10/31);  new base G (10/2);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 3.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCA  >  NC_000913/2120590‑2121053
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           
aTCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGGAAGCGGCGTGTTGTAAAGCGGCACCACCGGGACGCCGTTCCTCTCTTCGAGGGGTGGATGGGGAATGTTgga                                                                                                                                                                                                                                   >  1:934668/1‑237 (MQ=255)
    gCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGCGGACCCCCCGGTACGCCGTTCATTTCTTCCAGGCGGGGATGGGGAATGTTGAAGGTa                                                                                                                                                                                                                               >  1:947276/1‑239 (MQ=255)
      ttGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGGGGCCCCACCGGTACGCCGTTCATCTCTTCGAGGCGGGAATGGGGAATGTTGAAAGGaaa                                                                                                                                                                                                                             >  1:275251/1‑239 (MQ=255)
        gCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGTCAGCACAATGTCTTCCGGACGTTTGAGCAGGCGGTTAAACCCGGAAAGCGGGGGAGCGTAAAGCGGCACCCCCGGTACGGCGCTCACCTCCTCGAGGGCGCGAAGGAGAAGGCTGCAGGGaaaaa                                                                                                                                                                                                                           >  2:661488/1‑237 (MQ=255)
               atGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGTAAAGCGGCACCACCGGTACGCCGGTCATCTCTTCGAGGCGTGAATGGGGAATGTTGAAGGGAAAAACGTCggg                                                                                                                                                                                                                    >  1:247344/1‑239 (MQ=255)
                tGCCGTAGCGGGTCGGGCGGAAAATACCCGGCCTTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                                                                                                                                                                   <  1:1083219/239‑1 (MQ=255)
                ggcggtggCGAGTCTGGCGGAAAATACCCGGCCCGGGTAAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                                                                                                                                                                   <  1:863602/232‑1 (MQ=255)
                   cGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCCCTTCCAGGGGGGAGTGGGGAATGTTTAAGGGAAAGACCTCGGGGGTc                                                                                                                                                                                                                >  1:407794/1‑239 (MQ=255)
                     tAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAAACCCGGAAAGCGGCGTGTCGGAAAACGGCCCCACCGGTACGCCGTTCATCTCTTCCAGGCGTGAATGGGGAATTGTTAAGGGAAAGACGGCGGGGGTcag                                                                                                                                                                                                              >  1:1087918/1‑239 (MQ=255)
                     gaggcaGTCTGGGCGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATcag                                                                                                                                                                                                              <  1:399014/234‑1 (MQ=255)
                         gtgTCGGGGGGAAAATACCCCGCCCGGGGGAACTGGTTTTACCCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCagcagc                                                                                                                                                                                                          <  2:247344/237‑1 (MQ=255)
                           ttcaggCGGAAATAAACCGGCCCTGGTGAATGGAGTTTCCCCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAgcagca                                                                                                                                                                                                         <  1:757851/234‑1 (MQ=255)
                            tCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGGAAAGGTGCACCACCGGTACGCCGTTCATCTCTTCGCGGCGGGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGGTCaacagccac                                                                                                                                                                                                       >  2:855197/1‑236 (MQ=255)
                            tCTGGCGAAAAATACCCGGCCCTGGTGACCTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                        <  1:165824/238‑1 (MQ=255)
                               ggCGAAAAATACCCGGCCCTGGTAAACTGGGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGaa                                                                                                                                                                                                    <  1:715521/239‑1 (MQ=255)
                                                    gTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGGATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGGGGGGTCCGCCCGTTGa                                                                                                                                                                               >  1:573972/1‑239 (MQ=255)
                                                        accggttttCCCCGCCAGCGCAATCCAGCAACGCCCCGGGGAGATCAGGAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGa                                                                                                                                                                           <  2:1087918/233‑1 (MQ=255)
                                                         cTGAGTTCCACCGCCAGCGCAATACAGCACAGCCCCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGAc                                                                                                                                                                          <  1:429265/239‑1 (MQ=255)
                                                                          gccAATACCGACCAACACCGGGGGGGGCCGGAGCAGATAAGGCGCCGCCAGCAAAATGGCATCCTCGCGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGGGTGCCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGGCCAGTTTTTTCACTcgcg                                                                                                                                                         <  2:395733/237‑1 (MQ=255)
                                                                                       agcacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGGAAAGGGGGGGGCCGCCCGTTGTTGGGCCAGGTTTTTCCCCCGCGCGCCGCCGCCCAt                                                                                                                                            >  1:941945/1‑239 (MQ=255)
                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGgag                                                                                                                                                                                                                                            <  2:701813/141‑1 (MQ=255)
                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGgag                                                                                                                                                                                                                                            >  1:701813/1‑141 (MQ=255)
                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGGGTCCGCCCGTTGATGGACCAGGTTTTTTCCTCGCGCGCGGTGGCACAttt                                                                                                                                          >  1:588820/1‑239 (MQ=255)
                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGCGTCGTAAAGCGGCACCACCGGTAAGCCGGTCATCTCTTCGAGGCGTGAATGGGGAATGTTGAAGGGACAGACGTCGGGGATCAGCACAACCGAAAAGAGGGTGTCCGCCAGTGGGGGGACAAGTTTTTTCACCAGCGCGCCGGCGcacaatg                                                                                                                                          >  2:177755/1‑236 (MQ=255)
                                                                                          cccGGGGAGATCAGCCGCAAAATAAGCGGCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGGAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTg                                                                                                                                         <  2:559389/238‑1 (MQ=255)
                                                                                           ccGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTg                                                                                                                                         <  1:855197/238‑1 (MQ=255)
                                                                                             gggggcgtCAGCAGCAGAAAAAGGCGCGCCAGGCCAAGTTCTTCCGCACGTTTGGGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCAt                                                                                                                                      <  2:1072072/232‑1 (MQ=255)
                                                                                               ggAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATc                                                                                                                                     <  1:292315/238‑1 (MQ=255)
                                                                                                gagaTCCGCAGCAAAATAAGCCTCCCCAGCACAATGTCTTCCGCACGTTTGAGCAGGGGGTTAACCCCGGAAAGGGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                    <  2:955612/238‑1 (MQ=255)
                                                                                                gagaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                    <  1:316474/238‑1 (MQ=255)
                                                                                                   aTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                        <  2:410012/167‑1 (MQ=255)
                                                                                                   aTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                        >  1:410012/1‑167 (MQ=255)
                                                                                                        ccacaaaaTAAGCTTCCCCAGCACAATGTTTTCCGCACGTTTGGGCTGGCGGTGAACCCCGGAAAGGGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGGCGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGa                                                                                                                           <  2:931371/233‑1 (MQ=255)
                                                                                                                      tCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGGTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGGTTTAGACGTTTTGaag                                                                                                                 >  1:931371/1‑234 (MQ=255)
                                                                                                                        gCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCTCGCCGTCGCACATTTGCATCGCGATATAGCCGTTATGAATCtttc                                                                                                            >  1:198788/1‑236 (MQ=255)
                                                                                                                                     tttCCGCCCGTTTGAGCAGGGCGTTAACCCCGGAAAGGGGCGGTTCGTAAAGCGGCCCCTCCGGTACGCCGTTCATCTGTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTc                                                                                               <  2:275251/237‑1 (MQ=255)
                                                                                                                                           cccGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGAcc                                                                                        <  1:68273/237‑1 (MQ=255)
                                                                                                                                                      cggtggTTAACCCGGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCa                                                                              <  1:517542/234‑1 (MQ=255)
                                                                                                                                                                ccccGGAAAGCGGGGTTTCGTAAAGCGGCACCACGGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  2:407794/239‑1 (MQ=255)
                                                                                                                                                                ccccGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTTAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  1:788789/239‑1 (MQ=255)
                                                                                                                                                                     gAAAGCGGCGTGTCGTAAAGCGTCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGCATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGCACAGGTGGGTTCCGCCAGTTGGTGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCCCTTTCGCGTCCTCCACCAACTGTTCCAAGTTAACCGCCCAGTc                                                              >  2:518093/1‑239 (MQ=255)
                                                                                                                                                                                     aaaGCGGCACCCCCGGTACGCCGTTCATCTCTTCGAGCCGTGAATGGAAAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  1:644095/238‑1 (MQ=255)
                                                                                                                                                                                     aaaGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  1:887180/238‑1 (MQ=255)
                                                                                                                                                                                                  cGGTACTCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGt                                 <  1:726797/239‑1 (MQ=255)
                                                                                                                                                                                                         ccGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCCCGTCCTCGACCACCTGTTGCAGGTTACCCCCCCAGTCGTTCGAAACCCCCCCCGCTTTCGGGCGGTGGTaac                           >  2:254285/1‑237 (MQ=255)
                                                                                                                                                                                                              tctcttTTCGGGGCGTTATGGGGAAATGTTTAAGGGAAAGGCGTCGGGGATCAGCAGCCCCGAACGGGTGGGGCCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCcca                     <  2:934668/237‑1 (MQ=255)
                                                                                                                                                                                                               cccttttCGCGGCGGGAATGGAGAATGTTGAAGGGAAAGCCGTCGGGGTTCCGGACCCCCGAACAGGGGGGTGCCGCCAGTTGATGGACCCTTTTTTTCCCTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCccac                    <  2:941945/234‑1 (MQ=255)
                                                                                                                                                                                                                actcgTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCccacc                   <  1:254285/238‑1 (MQ=255)
                                                                                                                                                                                                                    ttCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCTTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGCCGTTCGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACtt               >  1:337331/1‑239 (MQ=255)
                                                                                                                                                                                                                      cGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGCTATAGACGTTATGAATCTTGCCCGCTTTCCGGTCCTCGACCCGCGGTTGCAGGTTACCCGCCCAGTCGTTAGAAACCCCGCCCCGTTTCGGGTGGAGGTAAACGCCACCCACTCCa             >  2:449307/1‑239 (MQ=255)
                                                                                                                                                                                                                      cGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGCCCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCCCCCGGCTTCGGGTGGGGGTAAAccccccccccccca             >  2:356716/1‑233 (MQ=255)
                                                                                                                                                                                                                       gAGGGGTGAATGGAGAATGGTGAAGGTAAAGACGGCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCaa            <  1:356716/239‑1 (MQ=255)
                                                                                                                                                                                                                        ggggCGTGAATGGATAATGTTGAAGGTAAAGGCGGCGGGGATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTCTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCaa            <  1:556639/237‑1 (MQ=255)
                                                                                                                                                                                                                                  tGGGGAATGGTTAAGGTAAAGGCGGCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCTGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCa  <  1:456335/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tGGAGATGGTTGAAGGTAAAGCCGTCGGGGATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCa  <  1:147043/239‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           
ATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCA  >  NC_000913/2120590‑2121053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: