Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,124,053 T→C 14.3% E60G (GAG→GGG)  glcG ← DUF336 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,124,0530TC14.3% 175.9 / 2.6 72E60G (GAG→GGG) glcGDUF336 family protein
Reads supporting (aligned to +/- strand):  ref base T (41/18);  new base C (2/10);  total (43/29)
Fisher's exact test for biased strand distribution p-value = 1.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGT  >  NC_000913/3123816‑3124289
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
ctcGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTtct                                                                                                                                                                                                                                                 >  1:561646/1‑238 (MQ=255)
  cGTTTTCGCTTATTTCGCCAACCCCGCTGCTGCCGCTTTCGCGTCCTGCCCTTCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCt                                                                                                                                                                                                                                              <  1:513209/239‑1 (MQ=255)
  cGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCCGTCACGAACGCGGGACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAATTTCACGACGCCCCATCGCGGCGGGACGCGCTTTccccg                                                                                                                                                                                                                                              >  2:552726/1‑238 (MQ=255)
  cGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTtct                                                                                                                                                                                                                                                 >  1:272424/1‑236 (MQ=255)
     tttcctttttttCCCCCACCCCCCTTCTGCCCCTTTCGCGACCCGCCCATCCTGTCCTCCGGTTAAACCAGAAACGCCCCCGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCTGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTgg                                                                                                                                                                                                                                            <  2:410198/229‑1 (MQ=255)
      ttCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTTCCCTgggc                                                                                                                                                                                                                                          >  1:1062714/1‑238 (MQ=255)
              tttCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATaa                                                                                                                                                                                                                                  >  1:410198/1‑238 (MQ=255)
                            ctgctgCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAACCGCAATCGgagc                                                                                                                                                                                                                   >  1:240276/1‑238 (MQ=255)
                                       ttCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCCCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCCCGCAATCGTCCa                                                                                                                                                                                                        >  1:173864/1‑238 (MQ=255)
                                              ccccccaagccTGTGTCCCGGTTAAACCAAAAACCCCCCCGGACCCAATAATTGTCCCAACCACAACAACCGGGACGCCGCCTGCCAGCGACGTTAATAACGGCGCTGTAACGAACGCGGTACGGCCGTTGTTCACCATCTCTGCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGGCCATGCGACtt                                                                                                                                                                                                <  2:341874/230‑1 (MQ=255)
                                                  cgcATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGGGCTTTCCCCTGGGAGGTTAAAGCCCCAATCGGCGCGCAATCGTCCAGGCCACTaaaa                                                                                                                                                                                             >  2:148057/1‑237 (MQ=255)
                                                            gCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAAAGCCAGCAGATg                                                                                                                                                                                  >  1:401374/1‑239 (MQ=255)
                                                                cGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGCCTTAACGCCAGCAGATGAcc                                                                                                                                                                               >  1:219121/1‑238 (MQ=255)
                                                                  gTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGGGATATAAGACGCAATCGACGCGCAATCGTCCATGCGGCCTAAACCCAGCAGGTGAccggc                                                                                                                                                                            >  1:339356/1‑237 (MQ=255)
                                                                     aaaCCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCCCTTTCTCCTGGGACACATAAGCCGCAATCGGCGCGCCATCCGCCAGGCGACTTAACCCCACCAAAGGAcccccgca                                                                                                                                                                         >  2:1008216/1‑237 (MQ=255)
                                                                            aaaCGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGCCGCCCCAGCGCGGCGGTACGGGCTTTCTCCTGGGGGATTTAAGCCGCAATCGGCGCGCCATCGCCCCTTCGACTTAACGCCACAAGAAGACCCCCGTcccgggca                                                                                                                                                                  >  2:154743/1‑232 (MQ=255)
                                                                                    aCGGCCCAAATAATTGCCCCACCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCCCGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAAt                                                                                                                                                          <  1:776257/239‑1 (MQ=255)
                                                                                             ataatTTGCCACACCACAACCACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGGGGTACGTCCGTTGTTCACCATCCCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGa                                                                                                                                                 <  1:289356/239‑1 (MQ=255)
                                                                                                  ttGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATTCGCCTTAACGCCAGCAGATGACCGCCGTCACCGGCAACAGCAATGGAAACCGACCAGt                                                                                                                                            >  1:1088828/1‑239 (MQ=255)
                                                                                                        aTCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTAtttt                                                                                                                                      >  1:96903/1‑239 (MQ=255)
                                                                                                         tCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTCGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATACACCGCAATCGGCGCGCAAACGTCCATGCAACTTAACACCAGCCGATGAACGCCACCACACGCAACACAAATGGAAAAAAAACAATAAttttt                                                                                                                                     >  2:298038/1‑239 (MQ=255)
                                                                                                         tCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGGTATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAACAGATGACCCCCGCCCTCGGCAAAAACAAAGGAAAAAGACCAGTGAttttt                                                                                                                                     >  2:462497/1‑239 (MQ=255)
                                                                                                         tCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGGACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAACACGACCAGTTAttttt                                                                                                                                     >  1:256166/1‑239 (MQ=255)
                                                                                                                     ggTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCCCtt                                                                                                                          >  1:797385/1‑238 (MQ=255)
                                                                                                                        aCGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATc                                                                                                                                                                                                             <  2:121032/152‑1 (MQ=255)
                                                                                                                        aCGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATc                                                                                                                                                                                                             >  1:121032/1‑152 (MQ=255)
                                                                                                                        aCGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCCGATGACCGCCGTCAACGGCAACCGCACTGGAAACAAACCAGTTATTTTTCTGCGCCCCTTCCt                                                                                                                       >  2:404492/1‑238 (MQ=255)
                                                                                                                                        aCGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTCCGGCaataat                                                                                                       >  1:88262/1‑238 (MQ=255)
                                                                                                                                         cGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAAtt                                                                                                      >  1:920636/1‑238 (MQ=255)
                                                                                                                                            taaaaccGGCGCAGTCACGAACGCGGTACGTCCGGTGTTCCCCTTCTTATAAAAGCCCTTAGTTCCACGACGCCCCCGCGGCGGGTTACGCTCTTTCCCCTGGGGAATAAAACCCGCAATCGGCGCCCAAACGTCCAGGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCAc                                                                                                  <  2:1062714/233‑1 (MQ=255)
                                                                                                                                                      gcAGTCACGAACGGGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTCCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTg                                                                                       <  1:96101/239‑1 (MQ=255)
                                                                                                                                                      cccgtcccagaCGGGGTTCCTCCGTTTTCCCCCCTCCCTGTATAGCCCTTAGTTTCCCGACGCCCCGGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAACCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTg                                                                                       <  2:401374/229‑1 (MQ=255)
                                                                                                                                                               aaCGCGGTACGTCCGTTGTTCACCATCCCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAg                                                                              <  1:552726/239‑1 (MQ=255)
                                                                                                                                                                    ggTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACCGCAATGGAAACAGACCAGTTATTTTTCTGCGCACCTTCCTGACCCCCGGCAATAATTGCCCCCGCCATTTGCTGGCTAaaaaat                                                                          >  2:14526/1‑236 (MQ=255)
                                                                                                                                                                    ggTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGaaag                                                                          >  2:259438/1‑236 (MQ=255)
                                                                                                                                                                    ggTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATg                                                                          >  1:420363/1‑238 (MQ=255)
                                                                                                                                                                    ggTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCCCTTCCTGACCTGCGGCACTAATTGCACTCGCCATTTTCTGGCTAAGactta                                                                         >  1:656898/1‑235 (MQ=255)
                                                                                                                                                                          tCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAg                                                                   >  1:677328/1‑239 (MQ=255)
                                                                                                                                                                                gtccACCATCCCTTCAAACCCCTTAGTTTCACGACGCCCCAGCGGGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCa                                                             <  1:404492/236‑1 (MQ=255)
                                                                                                                                                                                  tCACCTCCTCTTCATCCCCCTTAGTTTCCCCGCCCCCCGGCGAGGCGTTCCGCCCTTTCCACAGGGACTAATAGCCCCGACTCGGCGCGCGATCGTCCATGCGCTTTAGCGCCAGCCGATTACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTTACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCAtt                                                           <  2:240276/239‑1 (MQ=255)
                                                                                                                                                                                   cACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTTGACTAGCCATTTTCTGGGTAAGAATGACtttattttttattt                                                          >  2:929856/1‑239 (MQ=255)
                                                                                                                                                                                   cACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTg                                                                                       <  1:922525/210‑1 (MQ=255)
                                                                                                                                                                                   cACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTg                                                                                       >  2:922525/1‑210 (MQ=255)
                                                                                                                                                                                      cATCTCTTCGTAGCCCTTAGTTTCACGACCCCCCAGGGCGGCGGTACGCGTTTTCCCCGGGAAGATAAAAGGCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGt                                                       <  2:464310/239‑1 (MQ=255)
                                                                                                                                                                                           ctTCATAGCCCTTTGTTTCCCGCCCCCCCGGCGCGGCGGTACGGGTTTTCCCCGGGGAGATAAAAGCCGCAAGCGGCGCGCAATCGCCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTc                                                  <  2:920636/239‑1 (MQ=255)
                                                                                                                                                                                           ctTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATTCGACTTAACGCCCGCACATGACCGCCGTCATCGGCAACAGCAATGGACACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCCGCGGGCATCATTCACTCCGCCACTTCCTGCCTCAGCATGACTTTCGTTTCTATTTTGtttt                                                    >  2:629355/1‑235 (MQ=255)
                                                                                                                                                                                           ctTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTAt                                                    >  1:146378/1‑237 (MQ=255)
                                                                                                                                                                                                tAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCttt                                              >  1:965227/1‑238 (MQ=255)
                                                                                                                                                                                                tAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGAGGACCGCCGGCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTCCGGCAACAATTGCACTCGCCATTTGCGGGTTAAAACAGACTTTAATTTTTATTTGTTTATTACtttt                                             >  2:569325/1‑239 (MQ=255)
                                                                                                                                                                                                   cccTTAGTTTCACGACGCCCCAGCGCGGCGGTGCGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCCTCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCAATCGCCATTTGCTGGCTCAGCATGACTTTAGGTTTCATTTTGTTAATCCTTTTCaa                                          >  1:60943/1‑239 (MQ=255)
                                                                                                                                                                                                   cccTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCctc                                                                                                                            <  1:1056524/158‑1 (MQ=255)
                                                                                                                                                                                                   cccTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGAACGCCGTCACCGGCAACAGCAATGGAAACATACCAGTTATTTTTCTGCGCCTCTTCCTTACCTGCGGGAATAATTTCAACCGCCCTTTGCTGGCTACAAATGACTTTTGTTTTCATTTTGTTATTCCtttttca                                          >  2:346694/1‑236 (MQ=255)
                                                                                                                                                                                                   cccTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGCCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCctc                                                                                                                            >  2:1056524/1‑158 (MQ=255)
                                                                                                                                                                                                    cctttttttCCCGCCGCCCCTGCGCTGCGTTCCCCGTTTTCTCCGGGGGAAATAAACGCGTAACCGGCGCAAAATCGTCCAGGCGGCTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGTGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAg                                         <  2:420363/233‑1 (MQ=255)
                                                                                                                                                                                                      ttAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCa                                                                                                                                                                        <  1:885242/111‑1 (MQ=255)
                                                                                                                                                                                                      ttAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCa                                                                                                                                                                        >  2:885242/1‑111 (MQ=255)
                                                                                                                                                                                                      ttAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAggg                                       >  2:1039887/1‑239 (MQ=255)
                                                                                                                                                                                                        aGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACCGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGACCTCGCCATTTCTTGGCTAAGAATGACTTTAGTTTTCATTTTTTTATTCCTTTTCAAgg                                        >  2:18896/1‑236 (MQ=255)
                                                                                                                                                                                                        aGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTTTTCCTTTTCAAGGGCt                                     >  1:1066728/1‑239 (MQ=255)
                                                                                                                                                                                                             cACGACGCCCCAGCGCGGCGGTACGAGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCCGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGGTATTTTTCTGCGCCTCTTCCTGACCTGCTGCAATAATTGCACTCGCCCTTTTCTGGCTCAAGATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGATTGtt                                 >  2:491/1‑238 (MQ=255)
                                                                                                                                                                                                              acgacgCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTTTCCGCCTCTTCCTGACCCGCGGCACTAATTGCACCCCCCATTTGCCGGCTTAGAATGCCTTTAGTTTTCTTTTTGTTATTCCTTTTCCAGGGCTTGttat                               >  2:968105/1‑237 (MQ=255)
                                                                                                                                                                                                                    ccccAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAAAGACAGCAGATGACCGCCGTAATCGGCAACAGCAATGGAAAAAGACCAGATATTTTTCTGCGCCTCTTCCTGACCTCCGGAAATAAATGCACCCGCCATTTGCTTGCCAAGACTGAATTTAGTTTTCATTTTGTTATTACTTTTCAAGGGCTAGTTCTCAAAtt                         >  2:861868/1‑239 (MQ=255)
                                                                                                                                                                                                                     cccaggcgggcggTACGCGCTTTCCCCTGGGAAATTAAAGCCGCAACCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAAttt                        <  1:1008216/231‑1 (MQ=255)
                                                                                                                                                                                                                     cccAGCGCGGCGTGCCGCGTTTTAGCGTGGGAGATGTAAGCCGGAAGCGGTCCAAAAGCGTCCATGCGTCTTAACGCCAGCAGATGACCGCCGTCAGCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAAGGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAAttt                        <  1:989770/239‑1 (MQ=255)
                                                                                                                                                                                                                           gcggcgGTACGCGTTTTCCCCTGGGAGAATAAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATc                   <  1:148057/238‑1 (MQ=255)
                                                                                                                                                                                                                           gcggcgGTACGCCCTTTCCCCTGGGAAATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATc                   <  1:212297/238‑1 (MQ=255)
                                                                                                                                                                                                                            cggcggTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGttt                                                                <  1:637177/192‑1 (MQ=255)
                                                                                                                                                                                                                            cggcggTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGttt                                                                >  2:637177/1‑192 (MQ=255)
                                                                                                                                                                                                                              gcggTACGGGCTTTCCCCTGGGAGATATAAGCCGAAAGCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAg                <  1:154743/238‑1 (MQ=255)
                                                                                                                                                                                                                               cggTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGGCTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGGTATTTTTCTGCGCCTCTTCCTGACATGCGGCAATAATTGGACTCGCCTTTTGTTGGCTAAGAATGACTTTAGTTTTCATTTGTTTATTCCTTTTCAAGGGCTGGTTTTACAATTTCAATCAAGta              >  2:258107/1‑238 (MQ=255)
                                                                                                                                                                                                                                ggTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTg              <  1:259438/238‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcgTTTTCCCCGGGGAGATAAAAGCCCAAACCGGCGCCTAAGCGGCCGTGCGGATTAACGCCGGCAGATCACCGGCGTAATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGca         <  2:845221/239‑1 (MQ=255)
                                                                                                                                                                                                                                      cgttttCTCCTGGGAAATAAACGCCGAAATGGGCCCGCAATCGTCCATGCCGCTTACCCCCAGCAGGTTACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGGCCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGcaca       <  2:824890/236‑1 (MQ=255)
                                                                                                                                                                                                                                      cccTTTCTCCTGGGGGAAATAAAGCCGCAATCGGCGCGCACTGCTCCATGCGCTATAACGCCAGTAGTGGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGcac        <  2:719024/237‑1 (MQ=255)
                                                                                                                                                                                                                                       gcTTTTCCCTGGGAGAATTAAGCAGCAATCGGCCGGAAATGGTCCATGCGACTTAACGCCAGCAGATGCCCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGATAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGcaca       <  1:861868/238‑1 (MQ=255)
                                                                                                                                                                                                                                            ctcCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCATTGACGCACAGAGGt  >  1:824890/1‑238 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
CTCGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCGCAATCGGCGCGCAATCGTCCATGCGACTTAACGCCAGCAGATGACCGCCGTCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGT  >  NC_000913/3123816‑3124289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: