BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,101,255	C→T	9.8%	S149F (TCT→TTT)	sodA →	superoxide dismutase, Mn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,255	0	C	T	9.8%	306.4 / 4.9	120	S149F (TCT→TTT)	sodA	superoxide dismutase, Mn
Reads supporting (aligned to +/- strand): ref base C (19/87); new base T (10/3); total (30/90)
Fisher's exact test for biased strand distribution p-value = 3.20e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 121 total aligned reads displayed.

CCGTACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATG  >  NC_000913/4101018‑4101485

ccGTACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATtct                                                                                                                                                                                                                                          <  1:828500/239‑1 (MQ=255)

    acggcgcAACAACGCTGGCGGTCAAGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCGCCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCg                                                                                                                                                                                                                                       <  1:1291117/235‑1 (MQ=255)

      tGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTg                                                                                                                                                                                                                                    <  2:965653/239‑1 (MQ=255)

      cgcgcAACATCGCTGGCCGGCAAGCTAACCCCTGGCGGTGATGGGAAGGTCCAAAAAACGGCTGCACCCGGCGGGGTGGCCTGAAAGCGGCCATCGAACGTGACTTCGGCACCGTTGAAAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGGATGGATGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATACTCCGCTg                                                                                                                                                                                                                                    <  2:356991/238‑1 (MQ=255)

            acaacGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGt                                                                                                                                                                                                                              <  1:903465/239‑1 (MQ=255)

             caccGCTGGCGGTCACGCTAACCACCGCCGTTTGTGGAAAGGTCGGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTg                                                                                                                                                                                                                             <  2:499499/236‑1 (MQ=255)

                       ggTCACGCTAGCCCCAGTCTGTTCTGGAAGAGTCTGAAAAAAGGCACCACCCTGCCGGTGGACCTGAAAGCGGCTATCGAACGTGACTTCGGATCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTAttt                                                                                                                                                                                                                    <  2:262146/238‑1 (MQ=255)

                           aCGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCCCCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTgg                                                                                                                                                                                                                <  1:865264/238‑1 (MQ=255)

                                       gCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCCCCCGTCGGGGTGACCTGAAACCGGCTAGCGAACGTGACTTCGGCTCTGTTGATAACTGCAAAGCATAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGGAGCTATTTCTGGCGCTTCCGGCTTc                                                                                                                                                                                                   <  2:1036904/239‑1 (MQ=255)

                                       gCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTTTACTGCTAACC‑CGGGCTTCCCGCCGACGGGGGGAGGCATTTCTGGCAGTTTCGGCtt                                                                                                                                                                                                    >  1:1266045/1‑238 (MQ=255)

                                         cTGTTCTGGAAAGGTCTGAAAAAAGGCACCCCCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTc                                                                                                                                                                                                   <  1:1297113/237‑1 (MQ=255)

                                         cTGTCCCGGAAAGGTCTGAAAAAAGGCACCCCCCTGCTGGGTGTCCTGAAAGCGGCTATCGAACGTGTCTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTccc                                                                                                                                                                                                 <  2:1109747/239‑1 (MQ=255)

                                           gTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGGTTTTCCGCGGATGGGGTAAGCGTTTTTTGGGGCTTCCGGCTTccc                                                                                                                                                                                                 >  1:458981/1‑237 (MQ=255)

                                           gTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACA‑CGGATTTTCCGCTGAGGGGTGAAGCGATTTTTGGCGCTTCCGGGTTccccg                                                                                                                                                                                               >  1:1082559/1‑237 (MQ=255)

                                            ttCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGGGAAGCCATTTCTGGCGCTTCCGGCTTCCCGAt                                                                                                                                                                                              >  1:170759/1‑239 (MQ=255)

                                             tCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGCTAAACTGGCGGTGGTTTCTACTGCCAACC‑CGGATTATCCCCTGATGGGGGAAGCTATTTTTGGGGCTTCCGGCTTCCCGGtt                                                                                                                                                                                             >  1:237566/1‑239 (MQ=255)

                                             tCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGAtt                                                                                                                                                                                             <  1:1000240/239‑1 (MQ=255)

                                              cTGGAAAGGTCTGAAAAACGGCCCCACCCTGCAGGGTGACCCGAAAGCGGCTATCGAACGTGACTTTGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCTGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTa                                                                                                                                                                                            <  1:85688/239‑1 (MQ=255)

                                               tGGAAAGGTCGGAAAAAAGGCCCCCCCCGGCAGGTGGACGTGAAAGGGGCTATAGGACGTTACTTCGGCTCCGTTGATAACTGCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTAt                                                                                                                                                                                           <  2:1128399/239‑1 (MQ=255)

                                                ggAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGGGGGTTCTACTGCTAAAC‑AGGGTTCTCCGCTGATGGGTGAAGCCATTTTCTGCGCTTCCGGCTTCCCCGTTATg                                                                                                                                                                                          >  1:838375/1‑239 (MQ=255)

                                                   aaGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGc                                                                                                                                                                                       <  1:1181381/239‑1 (MQ=255)

                                                   aaGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGc                                                                                                                                                                                       <  1:412926/239‑1 (MQ=255)

                                                    aggttggAAAAAGGGCCCCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGGAGAATTTGAAAAAGCGGCAGTTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGc                                                                                                                                                                                       <  1:1109686/232‑1 (MQ=255)

                                                      gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTTTCCTGGTAACC‑CGGAATTTCCCCCGTTGGGTGAAGCATTTTCTGGGGCTTCCGGCTCCCCGCTTTTAGGccgt                                                                                                                                                                                    >  2:923802/1‑237 (MQ=255)

                                                      gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTTTCCCCTGTTGGGGGGAGCTATTTTGGGCGCTTCCGGGTTCCCGATTTAGGGccgg                                                                                                                                                                                    >  1:170305/1‑237 (MQ=255)

                                                      gTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACC‑AGGATTTTCCCCTGGTGGGGGAAGCTATTTCTGGTGCTTCCGGCTTCCCGATTATTGGCCTg                                                                                                                                                                                    >  1:298362/1‑239 (MQ=255)

                                                       tCTGAAAAAAGGCCCCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCt                                                                                                                                                                                     <  1:1104140/237‑1 (MQ=255)

                                                       tCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGATATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCt                                                                                                                                                                                     <  1:1260172/237‑1 (MQ=255)

                                                        ttGAAAAAAGGCACCACCCTGGAGGTTAACCTGAAAGGGGGTATCAAACGTGGCTTCGGCCCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGTTTCCCGCTTTGGTTCCGGCTGGGCATGGCGGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGa                                                                                                                                                                                  <  2:95175/238‑1 (MQ=255)

                                                        cTGAAAAAAGGCCCCCCCCTGCAGGGTGACCTGAAAGCGGCTCTCGGAGGGGACTTGGGCTCGGTTGAAAACTTGAAAGCAGAATTTGAAAAAGCGGGAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTgg                                                                                                                                                                                   <  2:1128219/238‑1 (MQ=255)

                                                        cTGAAAAAAGGCCCCCCCCTGCAGGGTGACCGGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGa                                                                                                                                                                                  <  2:170759/239‑1 (MQ=255)

                                                         ttaaaaaaGGCCCCGCCCTGCAGGGTGACCTGAAAGCCGCAATCGAACGTGACTGCGGCTCCGGTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGa                                                                                                                                                                                  <  2:1297701/236‑1 (MQ=255)

                                                         tGAAAAAAGGCCCCCCCCTGCAGGGGTACCTGAAGGCGGCTATCGAACGGGACTGCGGGTCCGGTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGAt                                                                                                                                                                                 <  2:698509/239‑1 (MQ=255)

                                                                aGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGCTAAACTGGCGGTGGGTTCTTCTTCTAACCAAGGATTTCCCCCTGTGGGGGGAAGCTATTTTGGGCGCTCCCGCCTCCCCCATAATGGG‑CTGGAtgtggtgg                                                                                                                                                                          >  2:30941/1‑235 (MQ=255)

                                                                   tacccccCTGGTGGGGGACATAAAAGCGGGTATGGAAAGTGACTTGGGGTCCGGTGAGAACTTCAAAGCAGAATTGGAAAAAGCGGCAACTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAGCC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGaa                                                                                                                                                                       <  2:586523/237‑1 (MQ=255)

                                                                   ccccaccCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTGGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGaa                                                                                                                                                                       <  1:414973/236‑1 (MQ=255)

                                                                    cccccccTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGCCTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACa                                                                                                                                                                     <  2:1071963/235‑1 (MQ=255)

                                                                    acccccCTGCAGGGTTACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAAc                                                                                                                                                                      <  1:994319/238‑1 (MQ=255)

                                                                          cTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCt                                                                                                                                                                                     <  1:901844/218‑1 (MQ=255)

                                                                                  tGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATTGGGCTGG‑GGTGTGGGGGACATCCTTAATACCTGa                                                                                                                                                       >  1:1028791/1‑239 (MQ=255)

                                                                                   taccgaaaaGCGGCCATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGa                                                                                                                                                       <  1:101554/232‑1 (MQ=255)

                                                                                   gACCTGAAAGCGGCTATGGAACGTGACTTGGGCTCCGTTGATAACTTAAAAGCAGAAGTTGAAAAAGCGGAAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGa                                                                                                                                                       <  1:49567/238‑1 (MQ=255)

                                                                                   gACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGGTAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTGCTGCTAACC‑CGGCATTTCCGCTGATGGGGGAAGCAATTTCTTGGGCATCCAGCGTTCCGATTATTGGCCTGGATGTGT‑GGGAAGATCGTTACTACCTGaa                                                                                                                                                      >  1:852952/1‑239 (MQ=255)

                                                                                   gACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGa                                                                                                                                                       <  1:5797/238‑1 (MQ=255)

                                                                                     ccTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTTCTGCTAACC‑AGGGTTCTCCGCTGGTGGGGGGAGGTTTTTTTTGCGCTTCCGGCTTCCCCGTTTTTGGCCTGGGGGGGTGGGGAACAT‑CTTATCACCCGAAAt                                                                                                                                                    >  2:1338086/1‑239 (MQ=255)

                                                                                               gttttCGAAGGTGCGTTCGTGTCCGTTGATATCTGCAAAGCGGAATTGGAAAAAGCGGCAGCTTCCCGCTTTGGTGCCGGTTGGGAAGGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTGCTACGGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCGGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGG‑GGGAACATGCTTACTACCTGAAATTCCAGAAccg                                                                                                                                          <  2:203968/235‑1 (MQ=255)

                                                                                                cTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGCGGGTTTTAATGCCTACC‑AGGATTTCCCGCTTAAGGGGCAAGCTATTTTTTGCGCTTCCCGCTTCCCCCTTATGGGCCGGGAGTGGG‑GGGGACAAGCTTACTACCTAAAATTCCAGAAcagc                                                                                                                                         >  2:426514/1‑239 (MQ=255)

                                                                                                   tCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGt                                                                                                                                      <  1:30941/239‑1 (MQ=255)

                                                                                                    cGAACGTGGTTCCGGCTCCGTTGATAACTGCAAAGCAGAATTTGAAAAAGGGGTAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTc                                                                                                                                     <  1:1043518/239‑1 (MQ=255)

                                                                                                    cGAACGGGTCTTCGGTTCCGTTTATAACTTCAAATCGAAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTc                                                                                                                                     <  2:598314/239‑1 (MQ=255)

                                                                                                      accGTGCCTTTGGCTCCGTTAATAACTTCAAGGCAGAATTTGAAAAGGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCg                                                                                                                                   <  2:1298878/237‑1 (MQ=255)

                                                                                                        ggTGACTCCGGCTCCGTTGATAACTTCAAAGCAGAATTGGAAAAAGGGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCGGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  2:298362/238‑1 (MQ=255)

                                                                                                        ctgtctttCGGCCCCTTTTATTACTTAAAAGCAGAATTTGAAAAGGCGGCAGCTTCCCGGTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTGCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTCCCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  2:578582/233‑1 (MQ=255)

                                                                                                        cGTGTCTTCGGCCCCGTTGAATATTTAAAAGCAGAATTTAAAAAGGGGGCGGCTTCCCGTTTTGGTTCCGGTTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  2:458981/239‑1 (MQ=255)

                                                                                                        cGTGCCTGCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAGGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  1:1338086/239‑1 (MQ=255)

                                                                                                        cGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  1:474644/239‑1 (MQ=255)

                                                                                                        cGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  1:298182/239‑1 (MQ=255)

                                                                                                        cGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  1:11073/239‑1 (MQ=255)

                                                                                                          tgccTTCGGCCCCTTTTATAACTGCAAAGAAGAATGTGAAAAGGCGGGAGCTTCCCGTTTTGGTTCCGGTTGGGCATGGCTGGTGCTGAATGGCGATAAACTGGCGGTGGTTTCTTCTGCTAACC‑AGGATTCTCCGGTGATGGGTGAAGCTATTTCTGGCTCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  2:597573/234‑1 (MQ=255)

                                                                                                                cggtgccGTTGATAACTTCAAAGCTGAATTTGAAAAAGCGGCACCATCCCGCTTTGGTTCCGTGTGGGCATGGCGGGGGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTa                                                                                                                         <  2:255989/234‑1 (MQ=255)

                                                                                                                  gCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGGGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGGTGTGT‑GGGAACATGCTTACTACCTGAAATTCCCGAACCCCCGTCCGGCCTTCATTaaa                                                                                                                       >  1:1233973/1‑239 (MQ=255)

                                                                                                                   ttCCGTTGATAACTTCAAAGCAGAATTTGAAAAGGCGGCAGCTTCCCGTTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAGCTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                       <  1:385811/237‑1 (MQ=255)

                                                                                                                   tccctttGTTAATTTCAAAGCAGATTTTGAAAAAGCGGCAGCTTCCCGTTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGACTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                       <  2:521654/233‑1 (MQ=255)

                                                                                                                   gaaggTTGATAACTTCAAAGGAGAATTTAAAAAGGGGGCAGTTTCCCGTTTGGGTTCGGGGTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                       <  2:472089/234‑1 (MQ=255)

                                                                                                                   cTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTaaa                                                                                                                       <  1:131410/238‑1 (MQ=255)

                                                                                                                      cggtGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTCCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCGGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGa                                                                                                                                 <  1:564843/222‑1 (MQ=255)

                                                                                                                      cGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTa                                                                                                                                                                                            <  1:81819/167‑1 (MQ=255)

                                                                                                                              aCTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAAc                                                                                                           <  1:923802/239‑1 (MQ=255)

                                                                                                                                 tCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtg                                                                                                        <  1:1068157/239‑1 (MQ=255)

                                                                                                                                 tCAAAGCAGAATTGAAAAAGGCGGCAGCTTCCCGGTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtg                                                                                                        <  1:426514/239‑1 (MQ=255)

                                                                                                                                  cAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGGTTCTCCGCTGATGGGTGAAGCTATTTCTTGCGCTTCCGGCTTCCCGCTTATTGGCCTGGATGGGT‑GGGAACATGCTTACTTCCTGCAATTCCAGAACCGCCGTCCGGACTCCATTAAAAAGATCTgtgaatg                                                                                                         >  1:472089/1‑231 (MQ=255)

                                                                                                                                     aGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGGGT‑GGGGACATGCTTACTACCTGAAATTCCCGAAACGCCGTCCGGGCTACATTAAAGAGTTCTGGAACGGGGG‑Ga                                                                                                   >  1:663859/1‑239 (MQ=255)

                                                                                                                                       aaaattttGAAAAAGCGGCAGCTTCCCGCTTTGGTGCGGGCGGGGCAGGCGTGGTGCTGAAGGGCGATAACCGGGCGGTGGTTTTTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGGGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAAc                                                                                                 <  2:1272069/234‑1 (MQ=255)

                                                                                                                                          aaTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGAt                                                                                                                                                                                 >  1:1253042/1‑158 (MQ=255)

                                                                                                                                            tttGAAAAAGCGGCTGCCTCCCGCTTTGGTTCCGGCTGGGCTTGGCGGGGGCTGAAGGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGa                                                                                            <  2:636681/239‑1 (MQ=255)

                                                                                                                                                                     ttGGTTCCTGCTGGGCATGGCTGGTTCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGTTGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTgcggcg                                                                  <  1:818493/239‑1 (MQ=255)

                                                                                                                                                                      tGGTTCCGGCTGGGGATGGCTGGTTCTGAAAGGCGAAAAACGGGCTGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGa                                                                 <  2:663859/239‑1 (MQ=255)

                                                                                                                                                                             ggCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTTTACTGCTAAAC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTTTGGCGCTTCCCGCGTCCCGGTTATGGGCCCGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAAACGCCGGCCCGGCTACATTAAAAAGTTTTGGGAAGGGGTGGAAAGGGGACAA‑AACACGGGCCCGTTTTGGGCGGaaaaaaa                                                           >  2:733287/1‑238 (MQ=255)

                                                                                                                                                                              gCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGGTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGAGGGGT‑GGGAACATGCTTAATACCCGAAATTCCAGAACCGCCCTCCGGGCTACCTTAAAGAGGTCTGGAACCTGGG‑GAACCGGGACGG‑AAAAACGGCACGGTTTTTGGGGAaaaaaaaa                                                         >  2:483010/1‑239 (MQ=255)

                                                                                                                                                                                         cTGGTGCTGAAAGGCGATAAACTGGCGGTGGGTTCTACTGCTAACC‑CGGGTTCTCCGCTGGTGGGTGAAGCTATTTCTGGCGATTCCGGCTTCCCCGTTTTGGGGCTGGCTGTGT‑GGGGACATGCTTAATACCTGAAATTCCCGAAACGCCCTCCGGCACACATTAAAACGTTTTGGAACGGGGG‑GAACTGGGGCGAGACGACGGGCCGGTTTTGGGGGGAAAAAA‑AAACATTTgccac                                              >  2:1234404/1‑237 (MQ=255)

                                                                                                                                                                                            gTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGGTTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTAGATGTGG‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCCTCCGGGCTACCTTAAAGAGATCTGGAACCTGGT‑GAACTGGGACGA‑AACAGCGGGACGGTTTTCGGCGAAAAAAAAAGAATTTggcgccc                                            >  1:925346/1‑237 (MQ=255)

                                                                                                                                                                                             tGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGGAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACCTGGG‑GAACTGGGACGA‑AGAAGTGGCACGTTTTTCGGCGAAAAAATCATACTTTGccgcccgc                                          >  1:525012/1‑239 (MQ=255)

                                                                                                                                                                                             ggcggAAAGGTGATAAACTGGCGGGGGTTTCTACTGCTAACC‑AGGATTCTCCGGTGATGGGTGAAGCTATTTCTGGGGGTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCctg                                           <  1:260715/234‑1 (MQ=255)

                                                                                                                                                                                                 taaaGGCCATAAATGGGGTGTTGTTTCTTCTGCTACCC‑CGGATGCTCCGCTGATGGGTTAAGCTATTTCTGGCTCTTCCGGCTTCCCGATAATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCa                                      <  2:945557/238‑1 (MQ=255)

                                                                                                                                                                                                  aaaGGGTATAAACGGGCGGTGGTTTCTACTGCTACCC‑AGGATTCCCCGCTGATGGGTGAAGCTATTTCTGGCCCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGGACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCa                                      <  1:499203/238‑1 (MQ=255)

                                                                                                                                                                                                   agggCGATAACTTTGCGGTTGTTTCTCCTGTTAACC‑AGGATTTCCCGGTGGTGGGTGAAGCTTTTTCTGGGGCTTCCGGCTTCCCGGTTATGGGCCTGGATGGGT‑GGGAAAGTGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAt                                    <  2:1233973/237‑1 (MQ=255)

                                                                                                                                                                                                   aaGGCGTTAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAt                                    <  1:159793/239‑1 (MQ=255)

                                                                                                                                                                                                   aaGGCGGTAAACTGGGGGTTGTTTCTACGGCTAACC‑GGGATTCCCCGTTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGGGT‑GGGAACATGCTTCCTCCCTGAAATTCCAGAACCGCCGTCCGGACTACAGTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAt                                    <  2:1071001/239‑1 (MQ=255)

                                                                                                                                                                                                   aaGGCGAGACACTGGGGGTGGTTGCTACGGCTAACC‑AGGATTTGTCGTTTATGGGTAAAGTTATTGCTGGCCCTTCCGGTTTCCCGATTAGTGGCCGGGATGTGG‑GGGAACTGGCTGACTGCCAGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAt                                    <  2:799164/239‑1 (MQ=255)

                                                                                                                                                                                                   aaGGCAATAAACGGGCGGTTGTTTCTACGGCCAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTGCTGGCGCTTCCGGTTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAAt                                    <  1:407624/239‑1 (MQ=255)

                                                                                                                                                                                                       cGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTc                                                                                                                                     <  1:1299810/140‑1 (MQ=255)

                                                                                                                                                                                                       cGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGa                                  <  1:733287/237‑1 (MQ=255)

                                                                                                                                                                                                             ccTGGGGGTGTTTTTTACTGTTTACC‑GGGTTTCTCCGCGGATGGTTGAAGCTATTTCAGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCTGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGtata                          <  2:237566/238‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACgtg                                                                                                        <  1:18317/163‑1 (MQ=255)

                                                                                                                                                                                                                ggCGGTTGTTTCTCCTGCTAACC‑AGGATTCGCCGCTGATGGGTGAAGCTATTTCGGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGc                       <  1:750282/239‑1 (MQ=255)

                                                                                                                                                                                                                 gCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGTGCCTGGATGGGT‑GGGGACATGCTTTCCACCTCAAATTCCAGAACCGCCGTCCCGACTACAt                                                                                                                           >  2:1299810/1‑140 (MQ=255)

                                                                                                                                                                                                                                 cTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGGTTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGCCGA‑AGCAGCGGCCCGTTTTGCGGCGTAAAAATATTCATTTGCCAACGGCTGCAATGAGGCGTATGGGCCGCAAATAACCTTaaaa      >  2:575063/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCACAGAGGCGTATAGGCCGCATATCATCTTaaaa      >  2:1062263/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCCTATCAGCTTaaaa      >  1:1044614/1‑239 (MQ=255)

                                                                                                                                                                                                                                 cTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTaaaa      >  1:734270/1‑239 (MQ=255)

CCGTACTGCGCAACAACGCTGGCGGTCACGCTAACCACAGCCTGTTCTGGAAAGGTCTGAAAAAAGGCACCACCCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACC‑AGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGT‑GGGAACATGCTTACTACCTGAAATTCCAGAACCGCCGTCCGGACTACATTAAAGAGTTCTGGAACGTGGT‑GAACTGGGACGA‑AGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATG  >  NC_000913/4101018‑4101485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`