BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,253,949	A→C	10.2%	V66G (GTG→GGG)	nupX ←	nucleoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,253,949	0	A	C	10.2%	318.3 / 2.1	126	V66G (GTG→GGG)	nupX	nucleoside permease
Reads supporting (aligned to +/- strand): ref base A (59/49); new base C (4/12); total (64/62)
Fisher's exact test for biased strand distribution p-value = 3.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

ATTTAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGCTTTCTG  >  NC_000913/2253735‑2254186

atTTAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAACCCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGTT‑CCGACCAGACAACCGAAGATAAATGCCCAACCCCCTCCGCGGTACGCCATCACTTTA‑TGCACGCCAAAAGCCACtt                                                                                                                                                                                                                         >  2:1293461/1‑239 (MQ=255)

atTTAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAATTAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACtt                                                                                                                                                                                                                         >  1:585862/1‑239 (MQ=255)

atTTAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACtt                                                                                                                                                                                                                         >  1:310982/1‑239 (MQ=255)

atTTAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCCCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAACCGACtt                                                                                                                                                                                                                         >  1:1338208/1‑239 (MQ=255)

   tAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACtttt                                                                                                                                                                                                                       >  1:881859/1‑238 (MQ=255)

     aTGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGTTAAATGCGCTACCCGCGCCGCTGTACGCCATACCTTTA‑TGCACGCCAAAAACGACTTTTTCa                                                                                                                                                                                                                    >  1:1083376/1‑239 (MQ=255)

       ctttccgggAAGATCCCGCCGAAAATTCCAATTAAAATCCCCATCACCCCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGAGAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGc                                                                                                                                                                                                                  <  2:1338208/232‑1 (MQ=255)

           tCTGGAAGATACCGCCGAGAATTCGAATTCAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGCTAAATCCTGCACCATCAAATAAGGAATCCATTTTCGGT‑CCGCCCCGAGCACCAAAGATAAATCCGCTACCCCGTTCGCGGTCAGCCATAACTTAA‑TGCACACACAAAGAGACTTTTTCATGGAcc                                                                                                                                                                                                              >  2:692406/1‑239 (MQ=255)

           tCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGCGAATACTCACCAGCGCGGTGACGAAGATACTTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGACGATAAATGCGCTACCCGCGTCGCTGTACGCCATACCTTTA‑CGCAAGCCAAAAGCGCCTTTTTCAGCGAc                                                                                                                                                                                                               >  2:1137590/1‑238 (MQ=255)

           tCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGAcc                                                                                                                                                                                                              >  2:274444/1‑239 (MQ=255)

           tCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCCCGTCCCTGTACGCCACACCTTTT‑TGCACGCAAAAACCGCCTTTTTCAGCGAc                                                                                                                                                                                                               >  2:1010637/1‑238 (MQ=255)

              ggaagtaccccccgAAAATTCGAATTAAAACCCCCAACCACCCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCaa                                                                                                                                                                                                           <  2:15109/228‑1 (MQ=255)

              ggaaagttccgacgAGAATTCGAATTAAAATCCCCATCACACCGATGGAGTAGAAAATTCTCACCAGCGCGGTGACGAAGATAATTGACGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCaa                                                                                                                                                                                                           <  2:111874/231‑1 (MQ=255)

              ggAAGATACCGGCGAAAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACGCCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCaa                                                                                                                                                                                                           <  1:409883/239‑1 (MQ=255)

                  gATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGCATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTCCGCCCTCACTTTA‑TGCACGCCAAAAGCGACATTTTCAGCGACCCacacc                                                                                                                                                                                                        >  1:1130222/1‑234 (MQ=255)

                   atcccgccgAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGccc                                                                                                                                                                                                      <  1:1243506/236‑1 (MQ=255)

                   aTACCCCCGAAAATTCGAATTAAAACCCCCATCCCACCTATTGAGTAGAGAATACCCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGccc                                                                                                                                                                                                      <  2:324286/239‑1 (MQ=255)

                    ttccgccgAGAATTCGAATTAAAATCCCCATCACACCGATGGAGTAAAGAATACCCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGcc                                                                                                                                                                                                       <  2:222267/235‑1 (MQ=255)

                     aCCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTg                                                                                                                                                                                                    >  1:15109/1‑239 (MQ=255)

                        tgcgAGAATTCGAATTAAAACCCCCATCACACCGATGGAGTATAGAATTCTCCCCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCGAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCg                                                                                                                                                                                                 <  2:225707/237‑1 (MQ=255)

                               aTTCGAATTAAAATCCCCATCACGCCGAGGGAGTAGAGAATACTCACCAGCGCGGTGACGAAAGTAATTGCCGGTAACGCCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTCCCCGCGTCGCTGTACGCCATCACGTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCa                                                                                                                                                                                          <  2:406529/239‑1 (MQ=255)

                                   caaTTAAAATCCCCCTCACACCGATGGAATAGAGAATACGCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGc                                                                                                                                                                                      <  1:1338974/238‑1 (MQ=255)

                                             ccccATTACCCCGGTGTAGTAGAGAATTCTCACCAGCGGGGTGACGAAGATAATTGCCGGTAACACCGTGAAACCAAAGATAAATCCTGCAACATAAAATAAGGTACCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCAAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATgccgc                                                                                                                                                                            <  2:965380/239‑1 (MQ=255)

                                                     ccccGATGTATTAGAGAATACCCCCCGGCGCGGTGACGAAGATAATTGCCGGTAACCCCCTGAACCCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACg                                                                                                                                                                   <  2:881859/237‑1 (MQ=255)

                                                     cacCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACg                                                                                                                                                                   <  1:274444/239‑1 (MQ=255)

                                                           tGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGt                                                                                                                                                            <  1:1010637/239‑1 (MQ=255)

                                                              agtagAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCa                                                                                                                                                                                          >  2:480430/1‑208 (MQ=255)

                                                              agtagAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCa                                                                                                                                                                                          <  1:480430/208‑1 (MQ=255)

                                                               gtagAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCCTAATGCCGCCA‑ATCACGA‑CCTGTAac                                                                                                                                                         >  1:271307/1‑238 (MQ=255)

                                                                tagAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAacac                                                                                                                                                       >  1:1123999/1‑239 (MQ=255)

                                                                tagAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGGAACCAAAACATAAACCCGCCA‑AACACCA‑CCttgaaaac                                                                                                                                                       >  2:1267740/1‑232 (MQ=255)

                                                                     aaTACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCCAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAAc                                                                                                                                                   >  1:494031/1‑238 (MQ=255)

                                                                     aaTACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAAc                                                                                                                                                   >  1:1050460/1‑238 (MQ=255)

                                                                     aaTACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGCAACACTAAc                                                                                                                                                   >  1:1057952/1‑238 (MQ=255)

                                                                     aaTACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGAAACCAAAGCATAAt                                                                                                                                                                                 >  1:627084/1‑210 (MQ=255)

                                                                     aaTACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCACGTCGCTGTCCGCCATCACTTTA‑TGCACGCCAAAATCGCCTTTTTCAACAACCCAACGCCCTGCCGGAAACACAACCATAATGCCGCCA‑ATCACGA‑CCGTGAACACTAAc                                                                                                                                                   >  1:1161750/1‑238 (MQ=255)

                                                                     aaTACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACAGAAGATAAATGCGCTACCCGCCTCGCTGTACGCCATCCCCTTA‑TGAACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGCCGGTCACCAAACAATAATGGTGCCACACCAC‑A‑CCTGCAACACCAAc                                                                                                                                                   >  2:1281903/1‑238 (MQ=255)

                                                                          tCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGTCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAAcgcccc                                                                                                                                              >  1:626652/1‑236 (MQ=255)

                                                                          tCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCACAAAGCCGCCA‑ATCACGA‑CCCGTAACACAAAcgaccc                                                                                                                                              >  2:415219/1‑234 (MQ=255)

                                                                          tCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGGATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCTTCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCAACCCAACGCCCTGCCGGTAACCAAACCATAATCCGGCCA‑CTCACGA‑CCTGTACAACTAAcccccg                                                                                                                                              >  1:828442/1‑236 (MQ=255)

                                                                            aCCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCCAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATTCCCCCA‑ATAACCA‑CCTGAAACACTAACCCCCCGcc                                                                                                                                           >  2:1371991/1‑239 (MQ=255)

                                                                               aGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ACAACGA‑CCTGTACAACTAAcccaccccccac                                                                                                                                        >  2:808989/1‑228 (MQ=255)

                                                                                 cgcgGTGACGAAGATAATTGCCGGTAACACCCTGACACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCTTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTCACCAAAGCATAATGCCGCCA‑AACACGC‑CCTGTACAACCAACGCCGCCCCAACgc                                                                                                                                      >  2:961272/1‑238 (MQ=255)

                                                                                 cgcgGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCACCGACCCAACGCCCTGCCGGTAACCCAAACATAATGACGCCA‑ATCACGC‑CCTGTCAAACAAACGCCccgcaacagg                                                                                                                                      >  2:710361/1‑233 (MQ=255)

                                                                                     gttaCGAAAATAATTGCCGGTAAACCCCTGAAACCAAAGATAAACCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  <  2:271307/236‑1 (MQ=255)

                                                                                      tGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGTTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  <  1:1281903/238‑1 (MQ=255)

                                                                                      tGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTACCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCCCTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  <  2:1017698/238‑1 (MQ=255)

                                                                                      tGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAAGCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCCGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  <  1:203933/238‑1 (MQ=255)

                                                                                      tGACCAATATAATTGCCGGTAACACCCGTAAACCAAAGATAAATCATGCCCCAACAATTAAGGTTTCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  <  2:585862/238‑1 (MQ=255)

                                                                                                aaTTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTaa                                                                                                                                                                                             >  2:175394/1‑171 (MQ=255)

                                                                                                aaTTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTaa                                                                                                                                                                                             <  1:175394/171‑1 (MQ=255)

                                                                                                        gTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAACTTCTTCTTg                                                                                                               >  1:859445/1‑239 (MQ=255)

                                                                                                               ccTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTTGAACACAAACCCCGCGCCAACGGTACGCAGGCTGATCTTTTTCTTGTTTACTg                                                                                                        >  1:684897/1‑239 (MQ=255)

                                                                                                                 tGAAACCAAAGATAAATCCTGCCACCTCAAATCAGGTATCCATTTGCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTGTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGAc                                                                                                      <  1:1254125/239‑1 (MQ=255)

                                                                                                                 tGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTGCCCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACCCTACAGCCGCGCCAACGGGACCCAGCCCAATTTTCTTTTTGTTTACAGAc                                                                                                      >  2:867107/1‑239 (MQ=255)

                                                                                                                     aCCAAAGATAAATCCTGCACCATCAAATAA‑GTATTCCTTTTCCGTCCCAACCAGAAAACGGAAATTAAATGCCCAACCCCCGTCCCTGTCCCCCTTC‑CTTTATTCCACCCCAAAACCACCTTTTTAACGAACCCAACCCCCT‑GCCGTAACCCAAACATAAAGCCCCCC‑ATCCCCACCCCTTAACCCACACCCCCCCCCAAGCGCACCCAGCCTGATCTTCTTCTTGtttcatccacaaa                                                                                                  >  2:609602/1‑229 (MQ=255)

                                                                                                                      ccAAAGAAAAATCCTGCCCCAACAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTaa                                                                                                 <  1:528317/239‑1 (MQ=255)

                                                                                                                         aaGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ACTCCGA‑CCTGCAACACTAACGCCCCCCCACCGGTAACCAGGCCGATCTTCTTCTTGTTTACTGACAGTaaaaa                                                                                              >  2:695022/1‑239 (MQ=255)

                                                                                                                                     gccccATCAAAAAAGGTATCCTTTTTCGGT‑CCGACCAAGAAACCGAAATAAAATGCGCTCCCCGCTTCGCTGTACGCCAGCCCTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACCCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAg                                                                                  <  2:1072986/236‑1 (MQ=255)

                                                                                                                                      cACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttctt                                                                                                                   <  2:1183928/205‑1 (MQ=255)

                                                                                                                                      cACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttctt                                                                                                                   >  1:1183928/1‑205 (MQ=255)

                                                                                                                                           tCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCCATa                                                                             >  1:555043/1‑238 (MQ=255)

                                                                                                                                           tCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCACTa                                                                             >  1:550906/1‑238 (MQ=255)

                                                                                                                                           tCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATa                                                                             >  1:1354756/1‑238 (MQ=255)

                                                                                                                                                 aaGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCAt                                                                      <  1:415219/239‑1 (MQ=255)

                                                                                                                                                     tATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCaa                                                                                                                                                                                                           >  1:356535/1‑104 (MQ=255)

                                                                                                                                                       tCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACCCACGCTGATCTTCTTCTTTTTTCCTGACAGTAAAAACGCAAACGCCAGAAAAACCACCATTCCCaa                                                                >  2:626061/1‑238 (MQ=255)

                                                                                                                                                       tCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCAACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAACACGCAATCGTCAGCAATACCACCATTCCCAg                                                                >  1:921293/1‑239 (MQ=255)

                                                                                                                                                         ccttttCGGT‑CCCGCCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGACATCACTTTA‑CGCACGCCAAAAGCGGCTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGaa                                                              <  1:813626/237‑1 (MQ=255)

                                                                                                                                                         cATTTTCCGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCTTCGCTGGACGGCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGaa                                                              <  1:115644/239‑1 (MQ=255)

                                                                                                                                                                 gT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  >  1:339843/1‑163 (MQ=255)

                                                                                                                                                                 gT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACg                                                                                                                                  <  2:339843/163‑1 (MQ=255)

                                                                                                                                                                    ccGCCCAGAGAACCCAAGTAAAATGGCCCACCCCGGTCGCTGTACGCACGCCCTTTA‑TGCCCGCCAAAAGCGGCTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCAt                                                    <  2:430976/239‑1 (MQ=255)

                                                                                                                                                                    ccGAACAGAGAACCGAAGATAAAGGCGCGACCCGCGTCGCTGTACGCTATCGCGTTA‑TGCACCCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCAt                                                    <  1:710361/239‑1 (MQ=255)

                                                                                                                                                                     cGACCAGAGAACCGAAGATAAATGCGCTTCCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCAt                                                    <  1:512257/238‑1 (MQ=255)

                                                                                                                                                                     cGACCAAAGAACCGAAAATAAATGCGCTTCCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCAt                                                    <  1:1125021/238‑1 (MQ=255)

                                                                                                                                                                       ccccgagaACCGAAGATAACTGCGCTCCCCTCGTCGCTGTACGCCATTACTTTA‑GGCACGCCAAAACCCAATTCTTCAGCCACCCAACGGCCTGGGCGTAACCAAACAAAAATGCCGCCA‑AACACGT‑CCTGTAACACTAACGCCGCGCCAACGGTTCGCAGGCTGATCTTCTTCTTGGTTACTGACAGTAAAAACGCAATCGTCAGCAAGACCACCATTCCCAGAACACTTCTCAGGAc                                                 <  2:1217564/235‑1 (MQ=255)

                                                                                                                                                                       aCCAGGAAACCAAAAATAAATGCGCTCCCCGCGTCGCTTTACCCCCTCCCTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCCTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGAc                                                 <  2:555043/239‑1 (MQ=255)

                                                                                                                                                                       aCCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCCACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACCGTAAAAACCCAATCGCCAGCAATACCACCATTCCCAGAACACTTCCCATGAc                                                 >  1:640906/1‑239 (MQ=255)

                                                                                                                                                                        cccgagaCCCGAAAATAAATGCCCTCCCCGGGCCGCTGTACCCCATCCCTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACa                                                <  2:859445/236‑1 (MQ=255)

                                                                                                                                                                        ccAGAAAACCAAAAATAAATGCGCTACCCGCTTCGTTGTACGCCACCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGCCCCAAAGACCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACa                                                <  2:310982/239‑1 (MQ=255)

                                                                                                                                                                            gaaaCCCAAAAAAAATGCCCTCCCCCGGGCCCTGTACCCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGCCCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCa                                            <  2:1123999/237‑1 (MQ=255)

                                                                                                                                                                            agaACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCa                                            <  1:62709/239‑1 (MQ=255)

                                                                                                                                                                              aaCCGAAGAAAAAGTCGCTACCCGCTTCCCTGTACCCCTTCCCTTTA‑GGCACCCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATa                                          <  2:626652/239‑1 (MQ=255)

                                                                                                                                                                              aaCCGAAAATAAAGTCCCCCCCCGCGGCCCTGGACCCCATCCCTTTA‑AGCCAGCCAAAAGCGAATTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCa                                            <  2:1050460/237‑1 (MQ=255)

                                                                                                                                                                                   aaGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGTCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCAt                                                                      <  2:537567/206‑1 (MQ=255)

                                                                                                                                                                                          atggcacaCCCGCGTCCCTGTCCCCCATCCCTTTA‑AGGCCCCCAAAAGCGACTTTTTCAGCGGCCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATctct                              <  2:640906/232‑1 (MQ=255)

                                                                                                                                                                                          aggcgcTACCCGCGTCGCTGGACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATctct                              <  1:1371991/237‑1 (MQ=255)

                                                                                                                                                                                          aTGGGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTCCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATctct                              <  1:781141/239‑1 (MQ=255)

                                                                                                                                                                                            gcccTACCCCTGTCCCTGTCCCCCCTCACTTTT‑TGCACCCCAAAAGCGACTTTTTCACCGACCCAACGCCCTTGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTc                            <  2:1173135/236‑1 (MQ=255)

                                                                                                                                                                                                  cccGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCAtaa                                                  >  1:74254/1‑209 (MQ=255)

                                                                                                                                                                                                         cGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGa                                      <  1:1014298/216‑1 (MQ=255)

                                                                                                                                                                                                            tttACGCCATCCCTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGa            <  1:1267740/237‑1 (MQ=255)

                                                                                                                                                                                                                     tCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAACAACGCAATCGTCAGCAATACCACCATTCCCAGAACCCTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTTGGCGACGGCtt      >  1:1159374/1‑236 (MQ=255)

                                                                                                                                                                                                                     tCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGAc                                                 <  2:582910/193‑1 (MQ=255)

                                                                                                                                                                                                                     tCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGAc                                                 >  1:582910/1‑193 (MQ=255)

                                                                                                                                                                                                                     tCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGGCCGCAAAACCACCATTCCCCGAACACTTCTCATGACCTCCATAATGATTATCTCTTCATGTCATATTGAGCGACGGGTTTc    >  2:1050745/1‑238 (MQ=255)

                                                                                                                                                                                                                      cACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATCGGGATTATCACTTCATGTCATATTGGGCGACGGCttttt   >  2:982800/1‑236 (MQ=255)

                                                                                                                                                                                                                      cACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGCTTTCTg  <  1:1336020/239‑1 (MQ=255)

                                                                                                                                                                                                                      cACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACGGACAGTAAAAACGCAATCGTCAGCATTACCACCATTCCCAAAAAACATCTCCTGCAAACCAAAGGTATTATCCCTCCATGCCAAATTTGGGGAAggaatttt   >  2:768786/1‑232 (MQ=255)

ATTTAATGCTTTCTGGAAGATACCGCCGAGAATTCGAATTAAAATCCCCATCACACCGATGTAGTAGAGAATACTCACCAGCGCGGTGACGAAGATAATTGCCGGTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGT‑CCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTA‑TGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCA‑ATCACGA‑CCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTCAGCAATACCACCATTCCCAGAACACTTCTCATGACATCCATAGTGATTATCTCTTCATGTCATATTGGGCGACGGCTTTCTG  >  NC_000913/2253735‑2254186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`