Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,417,249	T→G	13.6%	G739G (GGT→GGG)	acrF →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,417,249	0	T	G	13.6%	229.5 / 4.8	100	G739G (GGT→GGG)	acrF	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (31/50);  new base G (14/4);  total (46/54)
Fisher's exact test for biased strand distribution p-value = 3.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTGGAA  >  NC_000913/3417021‑3417480                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            tccattcAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGGGCGCCCTAATGGCCTGGAAGAAACCCCGCAGTTTAAACTGGAAGTTTGCCAGGGAAAGACGC‑CGGCATTAGGTGTTTCACttt                                                                                                                                                                                                                                   >  1:1052027/1‑239 (MQ=255)  cctttaaaTATGCCCGGCTTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTc                                                                                                                                                                                                                                  <  2:797571/233‑1 (MQ=255)             gccagtcaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACatcaat                                                                                                                                                                                                                        <  1:492597/238‑1 (MQ=255)               cagccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCCCGCAGTTTAAACTGGAAGTTTACCAGGAAAAAGCGC‑AAGCATTAGGGGTTTCCCTTTTTTACATCCATCAg                                                                                                                                                                                                                     >  1:98331/1‑239 (MQ=255)                 gccaTTGTTTAACGGGGCACGGCTACGGGTTTCGACTTGGAGTTAATTGATCAGGTGGGGCTGGGTAACGATGCCCGAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACCTCCTGCCAGCTTAGGCAGCGTGCGCCCTAATGGCCTGGAAGACCCCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGAc                                                                                                                                                                                                                   <  2:617893/239‑1 (MQ=255)                 gccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGAc                                                                                                                                                                                                                   <  1:604301/239‑1 (MQ=255)                  ccaTTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCCACATCCTTCCACCTTAGGCAACGTGCGCCCTAATGGCCTGGGAGACACCGCCCAGGTTAAACTGGAAGATGACCAGGAAAAGGCGC‑CGGCATTAGGGGTTTTAATTTCTGACATCAATCAAAcc                                                                                                                                                                                                                  >  2:791875/1‑239 (MQ=255)                      tgttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGAAACCGCGCAGTTTAAACTGGAAGTTGACCAGGCAAAGGCGC‑AGGCATTTGGGGGTTCACTTTCTGGCATCAATCAGACCA‑ttt                                                                                                                                                                                                             >  1:114547/1‑239 (MQ=255)                       gttgAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCCACATCCCGCCAGCCTAGTCAGCGTGCCCCCCAAATGCCCGGAAGAGACCGCGCCGGTTAAACTGGGAGGTGACCAGGAAAAGGCGC‑AGGGCATAGGGGTTTCACTTTTTGACAACAATAAGAACA‑att                                                                                                                                                                                                             >  2:492597/1‑238 (MQ=255)                              tGGGCACGGCAACGGTTTGCGAATTTGAGTTAAGTGAACAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCTCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     <  1:628564/239‑1 (MQ=255)                              tGGGCACGGCAACGGGTTTCGGCTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     <  1:333633/239‑1 (MQ=255)                              tGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     <  1:753944/239‑1 (MQ=255)                              tGGGAACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGGCATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     <  1:335466/239‑1 (MQ=255)                                ggCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATACTCCCAGCTTAGTCAGCTTTCGCCCCAATGGCCCGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGCCGC‑AGGCTTTAGGGGTTTCACATTCTGACATCAAACACACCA‑TTTTAAAGacgcc                                                                                                                                                                                                   >  2:786552/1‑238 (MQ=255)                                ggCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGACGCACCATCCTCCCACCCTAGTCAGCGTGCCCCCCAATGGCCTCGAAGACACCCCGCGGTTTAAACAGGCAGTTGACCAGGGCAAGGGGCGAGCCCTCAGG‑GGTTCACTTTCTCAGATACACCAAACCATTTTCAACgggag                                                                                                                                                                                                    >  2:971/1‑236 (MQ=255)                                    cGGGAACGGGTTTTGGCTTTGGGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCtgg‑gt                                                                                                                                                                                              <  2:1052027/239‑1 (MQ=255)                                     ggaaaCGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCtgg‑gtg                                                                                                                                                                                             <  1:615365/236‑1 (MQ=255)                                          cGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTTTGGCGGCGCAACCTCCTGCCAGCTTATTCACCGTCAGCCCTCATTGCCTGGAGAGCACCCCGCCGTTTTAACTGGAAAGTGCCTCGGAGAAGACGC‑AGGCCTTTGGGGGTTCCCATTCTGCACTCCAACAGACCA‑ATTTACcggggcggg‑ggtgggca                                                                                                                                                                                        >  2:105516/1‑232 (MQ=255)                                             gTTTCGACTTTGAGTTATTTGATCAGGCTGGGGTGGGTCACGATGCCCGAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACtt                                                                                                                                                                                       <  2:537361/237‑1 (MQ=255)                                                      ttGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCCGCCAGCTTAGTCAGCGGGCGCCCTAATGGCCTGGGAGACACCGCGCAGTGTAAACTGGAGGTTGTCCAGGAAAAGGCGC‑AGGCATTAGGGGTTTCACCTTCTCACATCAATCCAACCC‑TTTCAACGACGCTGG‑GGTGGACTTACGTGAAAGa                                                                                                                                                                             >  2:930492/1‑238 (MQ=255)                                                       tGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACAACCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCCGTTTAAACTTGAAATTGACCCGGAAAAGGCGC‑AGGCAGTATGGGTTTTAACTTTTGGCATTAAACAAACCA‑ATTCAACAGGCCTGG‑GGGGGAGTTAATTTaacaac                                                                                                                                                                            >  2:11813/1‑238 (MQ=255)                                                                       gctgggctggGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGCAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTACCTTTCTGACATCAATCAGACCC‑TTTCAACGGCGCTGG‑GGGGGGCTTACGTTAACGACTTCATCGACCGTGGccc                                                                                                                                                           >  1:96306/1‑238 (MQ=255)                                                                       gctgggctggGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCt                                                                                                                                                                                                   <  1:60482/200‑1 (MQ=255)                                                                          gggctgggTCCCGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCc‑gcg                                                                                                                                                        <  1:359969/238‑1 (MQ=255)                                                                              tgggTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑aa                                                                                                                                                   <  1:732750/238‑1 (MQ=255)                                                                                 gTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGATGACCAGGAAAAGGCGC‑AGGCATTAGGTGGTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTTAGTTAACGACTTCATCGCCCGTGGCC‑CCGTG‑CAAAAg                                                                                                                                               >  1:592573/1‑239 (MQ=255)                                                                                     cGATGCCCTACCCCAGGCCCGTTACCAGGTGCTTTGTATGGGGGCGCAACACCCCTCCAGCTGAGTCAGCGTGCGCCATAATGGCCTGGAAGACACCGCGACGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGt                                                                                                                                           <  2:280240/239‑1 (MQ=255)                                                                                     cGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCATGCGCCCTAATGGCCTGGGAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GGGGGGCTTACAGTAAAGACTTTAACAACCATGGCCGGGGGG‑AAAAAGtgg                                                                                                                                            >  2:419748/1‑237 (MQ=255)                                                                                          cccTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCCGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGGAAAGGCGC‑AGGCATTAGGGGGTTCACTTTCTGACATCCATCAAACCC‑ATTCAAAGGCGGCGG‑GGGGGACTTAAGTTAAAGACTTCATCGACCCGGGCG‑GCGGG‑gaaaaatggttagtg                                                                                                                                      >  2:955951/1‑229 (MQ=255)                                                                                               aCCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     <  1:996105/174‑1 (MQ=255)                                                                                               aCCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGc                                                                                                                                 <  1:419748/239‑1 (MQ=255)                                                                                                    ggCCCGTAACCAGTTGCTTGGTATGGCGGCGCCACATCCTGCCCGCTTAGTCAGCGGGCGCCCTAATGGACTGGAAAACACCGCGCAGTTTAAACTGGCAGTTGACCCGGCAAAGGAGC‑CAGCATTAGGGGTTTCACCTTCTTACCTCAATCAGACCA‑TTTCAACCGCGCTGGTGGGGGCCTTA‑GTTAAATACTCCTACGACCCTGGCC‑CCGGG‑ACAAAGTTGGATGATTAGGCgggg                                                                                                                             >  2:250285/1‑236 (MQ=255)                                                                                                     gCCCGTAACCAGTTGCTTGGTATGGCGGCGCCACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAAACACCCCGCAGTTTAAACTGGAAGTTGACCAGGTAAAGGCGC‑AGGCATTAGCGGTTTCACTTTCTGACATCAATCACAACA‑TTTCAACCGTGCTGG‑GTGGGACTTACGTTAAACACTTCATCGACCCTGGCC‑CCCTG‑TAACAGATGCATGTTCCGTCGGATg                                                                                                                            >  1:271183/1‑238 (MQ=255)                                                                                                     gCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAAACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGAACA‑TTTCAACGGCGCCGG‑GGGGGACTTACGTTAACCACTTCATCGACCGTCGCC‑CCGTG‑AAAAAATTGTATGTTGAGGCGGATg                                                                                                                            >  1:1038987/1‑238 (MQ=255)                                                                                                     gCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCCGCCAGCTTAATCCGCGTGCGCACCCATGGCCTTGGAGACACCGCGCCGGTTTAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGGGGTTCCCTTTCTGACATCCATCAGACGA‑TTTCAACGCCGCTGG‑GGGGGACTTCCGGTAAAGCCTTCTTCGACCGTGGCC‑CCGTC‑AAAAAATTGTTTGTTCAGGCggtggc                                                                                                                           >  2:159258/1‑235 (MQ=255)                                                                                                       ccGTAACCAGTTGCTTGGTTTGGCGGCGCAACATCCGGCCAGGTTAGTCCGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGGTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGcc                                                                                                                          <  2:616391/238‑1 (MQ=255)                                                                                                       ccGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGcc                                                                                                                          <  1:643422/238‑1 (MQ=255)                                                                                                                  ttcTTTGTAGGGGGGGGGAACATCCTGCCAGGTTAGTCCGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTAtg                                                                                                              <  2:98331/237‑1 (MQ=255)                                                                                                                  tggttGGTATGGCGGCCCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTAtg                                                                                                              <  1:105516/236‑1 (MQ=255)                                                                                                                           tGGGGGGGCAAAAACCTGCCAGCTTAGGTAGCGTGGGCCCTAAAGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTTTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCaga                                                                                                      <  2:604747/238‑1 (MQ=255)                                                                                                                            ggcggcGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGGGTTTCACTTTCTGAAATCAATCAGAACA‑TTTTAACGGCGCTGG‑GTGGGGCTTACGTTAACGACTTCTTCGCCCGTGGCC‑GCGGGAAAAAAGTTG‑ATGGTCAGTGGGGTGCCCAAATCCGGAAGCGACCagaaa                                                                                                    >  2:108955/1‑238 (MQ=255)                                                                                                                                 cgcAACATCCTGCCCGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGGCTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGATGTATGTTCAGGCGGATGCCAAATTCCGTATGCTTCCAGAAGAGGTc                                                                                               >  1:874902/1‑239 (MQ=255)                                                                                                                                   caccATCCTGCCAGCTTAGTCAGCGTGCGCCCTAAGGGCCTGGAAGACACCGCGGAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGa                                                                                             <  1:1021586/236‑1 (MQ=255)                                                                                                                                   cAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGGGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGa                                                                                             >  1:998826/1‑239 (MQ=255)                                                                                                                                     aCATCCTGCCAGGTTAGTCGGCGTGCTCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATa                                                                                           <  1:357535/239‑1 (MQ=255)                                                                                                                                     aCATCCTGCCAGCTTAGGCAGGGTGCGACCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATa                                                                                           <  2:803839/239‑1 (MQ=255)                                                                                                                                       ggggacaaaGCCTAGGCAGTGGGCGCCCTAATGGCCGGGAAGACGTCTCGAAGTTTAAACTGGAAGTTGAACAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGAAATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTAATCGACCGTGGAC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATGCCGTATGCTGCCAGAAGATGTCGATa                                                                                           <  1:729483/229‑1 (MQ=255)                                                                                                                                       aTCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAATTGTATGTTCAGGCGGATGCCCAATTCCGTAAGCCCCCAAAAAAGGGCGATaaa                                                                                         >  2:490103/1‑239 (MQ=255)                                                                                                                                             ccAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTAt                                                                                   >  1:313536/1‑239 (MQ=255)                                                                                                                                                 cTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGCCCGTGGCC‑GCGTG‑AAAAAGCTGTATGTTCAGTAGGATGCCAAATTCCCGATGCAGCCAGAAGATGTCGATAAACTTTagtccc                                                                               >  2:981824/1‑234 (MQ=255)                                                                                                                                                         gggggcgcCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGcc                                                                       <  1:747894/235‑1 (MQ=255)                                                                                                                                                          cGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTCAACTGGCAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCACTGG‑GTGGGACTTACGTTAACGACTTCATCCACCGTGGCC‑GCGTG‑ACAAAGTTGTATGTTCAGGCGGAGTCCAAAATCCGTGAGGTACCAGAAGAGGTCGATACACTTTTTTTcctgtatttt                                                                       >  2:729483/1‑230 (MQ=255)                                                                                                                                                          cGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGGCTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGGATGTTCAGGCGGAGTCCTAATTCCGGATGCGGCCTGAAAATTGTGATAAACTTTATGTCCGAGGCGCCa                                                                      >  2:71016/1‑239 (MQ=255)                                                                                                                                                                   tAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCCATTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGaaa                                                             <  2:598292/239‑1 (MQ=255)                                                                                                                                                                   tAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGaaa                                                             <  1:108955/239‑1 (MQ=255)                                                                                                                                                                           tGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATTTTCAGGCGGAGGCCAAATTCCGTATGCTGCCAGAAAATGTGGATAAACTTTATGTCCGCAGCGCCACCGGGGAAATGGGGCCa                                                     >  2:864751/1‑239 (MQ=255)                                                                                                                                                                           tGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCACAGGCGAAAAGGGGc                                                       >  2:731285/1‑237 (MQ=255)                                                                                                                                                                           tGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGc                                                       >  1:676890/1‑237 (MQ=255)                                                                                                                                                                           tGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAAAAAATGGCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGAGCCa                                                     >  2:151538/1‑239 (MQ=255)                                                                                                                                                                           tGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGCATGCTGCCAGAAAATGTGGATAAAATTTATGTCCGTAGCGCCAACGGGGAAATGGGGCCa                                                     >  2:847616/1‑239 (MQ=255)                                                                                                                                                                           tGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGAATGCTGCCAGAAGATGTCGAAAAACTTAATGTCCCCAGCGCCAAAGGCGAAAAGGGGcaa                                                     >  2:548043/1‑237 (MQ=255)                                                                                                                                                                             gAAGACCCCGGGCAGTTTAAACTGGAAGTTGGCCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGc                                                       <  1:541924/235‑1 (MQ=255)                                                                                                                                                                             gAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGCTGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCAt                                                    >  1:948929/1‑238 (MQ=255)                                                                                                                                                                                gACACCGCGCAGTTTAAACGGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATtct                                                 <  1:864751/238‑1 (MQ=255)                                                                                                                                                                                  cacCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGGGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCg                                               <  2:998826/238‑1 (MQ=255)                                                                                                                                                                                   acCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCCATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGCCCGTGGCC‑GCGTG‑AAAAAGATGTATGTTCAGGCGGCGGCCAAATTCCGTATGCTGCCGACAGATGTCCATCAACTTTAATTCCGCAGAGCCAACGGCGCAATGGAGCCATTCTCgg                                              >  2:667063/1‑238 (MQ=255)                                                                                                                                                                                   acCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCCAATTCCGTATGCTCCCAGAAGATGTCGATAAACTTTATGTCCGCAGCCCCAAAGGCGAAATGGGGCCATtctaga                                              >  2:1032049/1‑235 (MQ=255)                                                                                                                                                                                     cgcgCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     >  2:162239/1‑88 (MQ=255)                                                                                                                                                                                     cgcgCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGgcg                                                                                                                                                                                                     <  1:162239/88‑1 (MQ=255)                                                                                                                                                                                       cgcAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑GAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAAATGTCGATAAACTTTTTGTCCGCAGCGCCAACGGGGAAAAGGGGCCCTTCCCGGccctg                                         >  2:541924/1‑236 (MQ=255)                                                                                                                                                                                       cgcAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCt                                           >  1:663884/1‑237 (MQ=255)                                                                                                                                                                                       cgcAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCt                                           <  2:663884/237‑1 (MQ=255)                                                                                                                                                                                       cgcAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAAAGGGGCCATTTCGGGCCtta                                         >  2:525504/1‑238 (MQ=255)                                                                                                                                                                                       cgcAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGAATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGCTGTCGATACACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCttt                                         >  1:127839/1‑239 (MQ=255)                                                                                                                                                                                         cAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTa                                        <  1:11813/238‑1 (MQ=255)                                                                                                                                                                                             ttAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGGCCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCAc                                    <  1:981824/238‑1 (MQ=255)                                                                                                                                                                                              tAAACGGGAAGTTGGCCAGGAAAAGGCGC‑AGGCATTGGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCAc                                    <  1:949748/237‑1 (MQ=255)                                                                                                                                                                                                  cTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACAACAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTcaca                              <  1:786552/239‑1 (MQ=255)                                                                                                                                                                                                   tGGAAGTTTGCCAGGAAAAGGCCC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAt                             <  2:948929/239‑1 (MQ=255)                                                                                                                                                                                                   tGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACAACAATCAGACCA‑TTTCAACGGCGCTTG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAt                             <  1:1032049/239‑1 (MQ=255)                                                                                                                                                                                                    ggAATTTGACCAGGGAAAGGGGC‑GGGCATTTGGGTTTGCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAtt                            <  2:824798/239‑1 (MQ=255)                                                                                                                                                                                                    ggAAGTTTCCCAGGAAAAGGCGA‑GGGGATTAGGGGTTTACCTTTCTGACATCAATCAGACCA‑TTTCAACGGCGGTGG‑GTGGGACTTACGTTAACGCCTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGTATGCCAAATTCTGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTAGCTCTTCACAtt                            <  2:116883/239‑1 (MQ=255)                                                                                                                                                                                                    ggAAGTTGCGCATGAAAAGGCCC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGGCCA‑TTTCAACGGCGCTGG‑GTGGGACTTCCGTTACCGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCTCTTCACAtt                            <  1:357186/239‑1 (MQ=255)                                                                                                                                                                                                    ggAAGTTGACCAGGAAAAGGCGC‑AGGCATTATGTTTTTTCCTTTCTGACATCAATCAGGCCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAtt                            <  1:761018/239‑1 (MQ=255)                                                                                                                                                                                                    ggAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAtt                            <  1:151538/239‑1 (MQ=255)                                                                                                                                                                                                    ggAAGTTGAACAGGAAACGGGCT‑AGGCATTAGGTGTTTCACTTTCTTACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTTCGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAtt                            <  1:250285/239‑1 (MQ=255)                                                                                                                                                                                                    ggAAGGTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCCCTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACAtt                            <  1:525504/239‑1 (MQ=255)                                                                                                                                                                                                      aaGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTgg                          >  1:1003021/1‑239 (MQ=255)                                                                                                                                                                                                      aaGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTAGGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCACCGCCAACGGCGCAATGGTGCCATTCTCGGCCTTTACCCCTTCCCCTTgg                          >  2:1057412/1‑239 (MQ=255)                                                                                                                                                                                                      aaGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTAACTTTCTGACATCAATCAGACCA‑TTTCAAAGGCGCTGG‑GTGGGACTTACGTTAACGACTTAATCGACCATGGCC‑GCGTG‑AAAAAGTTGGATGTTCAGGCGGATGCCAAATTCCGGATGCGGCCAGAAGATGTAGGTAAACTTTATGTCCGCAGCGCCAACGGGGGAATGGGGCCATTCTCGGCCTTTACCACttaccatagg                          >  2:587435/1‑231 (MQ=255)                                                                                                                                                                                                          tgcccAGGAAAAGGGGC‑AGGCCTTGGGTGTTTTCCTTTTTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTTGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGgtgt                      <  2:114547/236‑1 (MQ=255)                                                                                                                                                                                                              cAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATgg                  <  2:676890/239‑1 (MQ=255)                                                                                                                                                                                                               aGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAAATGTCGGTAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGGGCCATTCTCGGCCTTTACCACTTCAAATTggggggaaggc                 >  2:400159/1‑231 (MQ=255)                                                                                                                                                                                                                            aGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTg     >  1:87884/1‑239 (MQ=255)                                                                                                                                                                                                                            aGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCCCTCCCCGCCTg     >  1:1060168/1‑239 (MQ=255)                                                                                                                                                                                                                             ggCATTAGGTGTTTCATTTTCTGACATCAATCAGACCA‑TTTCAACGGGGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTgg    <  1:930492/239‑1 (MQ=255)                                                                                                                                                                                                                                gttgggTTTTTCTCTTTCTGACAGTCATCCGACCC‑TTTCACCGGCGGTGG‑GTGGGACTTACGTTAACGACTTCATCGCCCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTGGaa  <  2:812681/234‑1 (MQ=255)                                                                                                                                                                                                                                cttgggTTTTTCACTTTTTGACACCAATCAGACCA‑GTTCAACGGGGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTGGaa  <  2:313536/234‑1 (MQ=255)                                                                                                                                                                                                                                aTTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTGGaa  <  1:791875/238‑1 (MQ=255)                                                                                                                                                                                                                                aTTAGGGTTTTCACTTTCTGACATCAATCAGACCA‑TTCCAACGGCGCTGG‑GTGGGATTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTTTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTGGaa  <  1:667063/238‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            TCCATTCAATATGCCAGCCATTGTTGAACTGGGCACGGCAACGGGTTTCGACTTTGAGTTAATTGATCAGGCTGGGCTGGGTCACGATGCCCTAACCCAGGCCCGTAACCAGTTGCTTGGTATGGCGGCGCAACATCCTGCCAGCTTAGTCAGCGTGCGCCCTAATGGCCTGGAAGACACCGCGCAGTTTAAACTGGAAGTTGACCAGGAAAAGGCGC‑AGGCATTAGGTGTTTCACTTTCTGACATCAATCAGACCA‑TTTCAACGGCGCTGG‑GTGGGACTTACGTTAACGACTTCATCGACCGTGGCC‑GCGTG‑AAAAAGTTGTATGTTCAGGCGGATGCCAAATTCCGTATGCTGCCAGAAGATGTCGATAAACTTTATGTCCGCAGCGCCAACGGCGAAATGGTGCCATTCTCGGCCTTTACCACTTCACATTGGGTGTATGGCTCTCCGCGACTGGAA  >  NC_000913/3417021‑3417480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑