Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,479,972 G→C 18.7% G212G (GGC→GGG kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,479,9720GC18.7% 132.2 / 2.3 66G212G (GGC→GGGkefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base C (11/3);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 3.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGC  >  NC_000913/3479739‑3480203
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
tGGGTTTAACACCCCCCGGGGTTGAGTGACTGAGCGAAAGAAAAAAAGAACAAACCGAGCAAGCAGCTTTTGAAGGGATCGATGGCCGTTTCCAGTTCAGGGCGATTTTCACTTCCCGCCAACAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGa                                                                                                                                                                                                                                          <  2:28212/239‑1 (MQ=255)
  ggTAAAAAGCCCCCCGGGGTTGAGTGACATGCCGACAGAAATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGa                                                                                                                                                                                                                                          <  2:30712/237‑1 (MQ=255)
      taAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCACCAGCCGCGTCGCGCCGGTGAACACTTCCCGCAccccctaacaccc                                                                                                                                                                                                                                     >  1:432658/1‑230 (MQ=255)
        aaGCCCCCGGAGGTGAAGTACCAGGCCGACAGAGAAAAAAAACAACCCGGGCAGCAAGCCTTGAGAGGGGTCGATGGCCTTTTCCATTTCATGGCGAAAATCACTTTCCGCCAGCAGGACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCaa                                                                                                                                                                                                                                 <  2:161407/239‑1 (MQ=255)
            aCCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATa                                                                                                                                                                                                                               <  1:259937/237‑1 (MQ=255)
             ccccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGACCACTTCCCGCCACCCCGAAGCCGCAATaaac                                                                                                                                                                                                                            >  1:1067744/1‑238 (MQ=255)
             ccccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACCCCAGAAGCTGCAATAAAg                                                                                                                                                                                                                            >  1:883915/1‑239 (MQ=255)
              cccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATTCGGAACCCACCACCAGCAGCAGCGTCGCGGCGGGGAACCATTCCCGCACCCCCAAACCGCCAATAAAGc                                                                                                                                                                                                                           >  1:88910/1‑239 (MQ=255)
              cccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGc                                                                                                                                                                                                                           >  1:59419/1‑239 (MQ=255)
              cccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGGGAACAATTCCCGCCCCCCAGAAGCTGCAAAAAAGc                                                                                                                                                                                                                           >  1:969105/1‑239 (MQ=255)
              cccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGGGAAAACTTCCCTCACGCCCCAAACTGCAAGaaaac                                                                                                                                                                                                                           >  1:719150/1‑237 (MQ=255)
              cccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGGTTCCAGTTCATGGCGATTTTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCCCGGCGGTGAACACTTCCCGCACGCCCGAAACTTCAATAAAGc                                                                                                                                                                                                                           >  1:400487/1‑239 (MQ=255)
               ccGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCg                                                                                                                                                                                                                          <  1:571175/239‑1 (MQ=255)
                cggggTTGAGTGACATGCCGACAGAAAAAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCgg                                                                                                                                                                                                                         <  2:301928/236‑1 (MQ=255)
                cGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCACCACCAGCACCAGCGCCCCGCCGGTGAAACCTTTCCCGACCCCCGCAACTGCAAAAAAGCgg                                                                                                                                                                                                                         >  1:543014/1‑239 (MQ=255)
                cGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCACAACCAGAACCACCGCCCCCGCGGGGAACACCCCCCCCCCCCCAAAAACGGCaaaaaaaggg                                                                                                                                                                                                                         >  2:1010906/1‑235 (MQ=255)
                        aGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGCGGCGGTGAAAACATCCCCCCCCCCCGAAGCTCGCAAAAAACAGAAAACCgg                                                                                                                                                                                                                 >  2:843709/1‑239 (MQ=255)
                        aGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACCCCAAAAGCTGCAAAAAAACGGAAAACCgg                                                                                                                                                                                                                 >  1:877044/1‑239 (MQ=255)
                           tactggCCGACAGAGATAAAGAATAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCCTCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGAc                                                                                                                                                                                                               <  2:357181/233‑1 (MQ=255)
                           gaaattcccaCAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGAc                                                                                                                                                                                                               <  1:549828/229‑1 (MQ=255)
                           gACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCCCTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACg                                                                                                                                                                                                              <  1:1010906/239‑1 (MQ=255)
                                        aaaTAAAAAACAAACCGAGCAGCAAGCCTTTGAAGGGAACGATGGCCTTTTCCAGTTCATGGCGATAGTCCCTTCCCGCCAGCAGCACGCCCGCAATAAATGACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATCCCGGACGCAGTAAATAGCGcc                                                                                                                                                                                                <  2:598325/237‑1 (MQ=255)
                                           tAAAGAACAACCGCATCAGCAAGCCTTTGAAGGGATCGGTGGGCGTTTCCGGTTCAAGGAGATTTTCACTTTCCGCCAGCATCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCCTCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGTGcc                                                                                                                                                                                                <  2:729643/237‑1 (MQ=255)
                                            aaaGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACCCCCGAAGCTGCACTAAAGCGGAATACCGGACGCAGTAAATCGCCCccac                                                                                                                                                                                             >  1:380408/1‑239 (MQ=255)
                                                    aaCCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGa                                                                                                                                                                                                                                          <  2:471350/187‑1 (MQ=255)
                                                    aaCCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCCCACGCCCCAAACGGCAATAAACCGGAATACCGGAACCCGTAAAAAACGCccacccaccacc                                                                                                                                                                                     >  1:1027823/1‑232 (MQ=255)
                                                    aaCCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACAATTCCCGCACGCCAGa                                                                                                                                                                                                                                          >  1:471350/1‑187 (MQ=255)
                                                    aaCCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCCGCGGTGAAAACTTCCCGCACCCCCGAAGCTGCAATAAAACGGAATACCGGACGCACTAAATAGCGCccaccacccacc                                                                                                                                                                                     >  1:296231/1‑233 (MQ=255)
                                                                                       gTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCCCCCCAGAAGCTGCAATAAAACGGGATACCGGACGCAGTAAATAGCCCCCCCCCATCACCAAGCCCCCAAACCCCCGCCCCCCCATGCCGccccc                                                                                                                                                  >  2:783796/1‑237 (MQ=255)
                                                                                          tCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCTAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAAAACCGGACGCAGGAAATAGCGCCCACCAATCAGCATGCCTGCAAACGCCAGCACCTTCATGCCGACCTTCa                                                                                                                                                >  1:18259/1‑238 (MQ=255)
                                                                                                  aTGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCCTGCCGACCTTCCTCCCGTCGa                                                                                                                                       >  1:545598/1‑239 (MQ=255)
                                                                                                       tatatTCCTTTTCCGCCAGCAGCCCGCCCCCAAAAAATGTCCCGCGCGCCAGCGACAGCCCCAGCGGATCCATAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATg                                                                                                                                   <  2:59419/237‑1 (MQ=255)
                                                                                                       gATATTCCCTTTCCGCCAGCAGCCCGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATg                                                                                                                                   <  1:356444/238‑1 (MQ=255)
                                                                                                                                          aaagtcccGGGCGCCATCGACCGCCCCAGCGCATCCAAAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                                            <  1:755734/233‑1 (MQ=255)
                                                                                                                                          aaTGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                                            <  1:287102/239‑1 (MQ=255)
                                                                                                                                                ccGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCCGCACCTTCATGCCGACCTTCATCCAGTCGCAATGTTCGTCTGCCGACCCCGCCAAAACCGCGCATAACGCCAggg                                                                                       >  1:700733/1‑236 (MQ=255)
                                                                                                                                                ccGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCCCCAACAAAGGCACTAACGcccaggc                                                                                      >  1:173533/1‑234 (MQ=255)
                                                                                                                                                ccGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGGAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAATCGAAAAGTTGGTCTTCCGCCCCCGCCACCAACGGCCCTAAAGcccgggc                                                                                      >  1:745318/1‑234 (MQ=255)
                                                                                                                                                 cGAGCGCCATCGACAGCCCCAGGGCATCCAAAAACAATGCGGAACCCAACCCCAGCAGCAGCTTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGCCCTTCATCCAGCCGAAATGTTCGGCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCt                                                                                     <  1:708840/239‑1 (MQ=255)
                                                                                                                                                  gAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAACATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGATCGTCTTCCGACCCCGCCAACAACCGCACTCACGCCAgcactg                                                                                    >  1:713425/1‑234 (MQ=255)
                                                                                                                                                       ccatgcgaaGCCCCAGGCCATCCCAAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGCGGCGGTGAACCCTTCCCGAACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGAt                                                                               <  1:756628/230‑1 (MQ=255)
                                                                                                                                                        cagcGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTa                                                                             <  1:843709/236‑1 (MQ=255)
                                                                                                                                                        cATCGACAGCCCCAGCGCATCCATAAACAAGGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACCCTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTa                                                                             <  1:259120/239‑1 (MQ=255)
                                                                                                                                                              cccccccCGCCCAACCACAAAAAAAGCGGAACCCACCCACAGCAGCTGCTCGCCGGCGGGGACACCTTCCCACCCCCCAGAACCTGCAAAAAAGCGGAATACCGGACGCAGGAAAAAGCGCCCACCAATCAGCATGCCGCCAAACGCCAACACCTTCAGGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGCCCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                                      <  2:691583/236‑1 (MQ=255)
                                                                                                                                                               aGCCCCAGGGCAACCATAAACAATGCGGAACCAAACACCAGCAGCAGCGTCGCGGCAGTGAACACTTCCCGCACGGCAAAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCCCCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCAGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                                      <  1:172884/238‑1 (MQ=255)
                                                                                                                                                                  cccAGGGCATCCAAAAACAATGCGGAACCCAACCACAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                                      <  1:1025537/235‑1 (MQ=255)
                                                                                                                                                                     aGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAAAAGCGCCCCCCAATCAACATGCCGACAAACGCCAGCCCCTTCATGCCGACCCTCCTCCACTCGAAAACGTCGTCTCCCGACCCCCCCCCCAACCGCACTAACCCCCATGCGGGGGTTACTggccggaccgg                                                                >  2:1084295/1‑229 (MQ=255)
                                                                                                                                                                      gcgcATCAATAAACAATGCGGAACCCAACCCCAGCAGCAGCGTCGGTGCGGTGAACACTTCCCGCACGCCAGAAGCGGCAATAAAGCGGAATCCCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGa                                                              <  1:209072/239‑1 (MQ=255)
                                                                                                                                                                                      tGCGGAACCCAACACCAGCAGCGGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCa                                                                                          <  1:864806/197‑1 (MQ=255)
                                                                                                                                                                                      tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCCGAAGCTGCAATAAAGCGGAATACCGGACGCCGTAAATAGCGCCCACCAATCAGCATGCCGGCAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGCCTGCCCACCCCCCCGCAAACCGCCACTACGCCCGCGGTGGGGATTACGCCAGTGCCGGAAACAACAGAACCaaaa                                              >  2:78303/1‑239 (MQ=255)
                                                                                                                                                                                      tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCCCTAACGCCCGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGaaa                                              >  1:264428/1‑239 (MQ=255)
                                                                                                                                                                                      tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCCATGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGaaa                                              >  1:969893/1‑239 (MQ=255)
                                                                                                                                                                                      tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAACGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGACCTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCCGAACCGaaa                                              >  1:252215/1‑239 (MQ=255)
                                                                                                                                                                                      tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACCTCCCGCTCGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCCCCCACCAATCAGCATGCCGACAAACACCACCACCTTCATGCCACCCCTCCCCCAGTCGAAATGTTCCGCTTCCGCCCCCGCCCAAAAACGCACTCAACCCCCTGCTCGGGTTAATCCCCAATCCTGAAACACCAGAAccccaa                                              >  2:197936/1‑235 (MQ=255)
                                                                                                                                                                                                aaCACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAACGCGGGATACCGGACGCAGTAAATAACGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCCTCCTCCCGGCAGAATGTTCGTCTGACGCCCCCCCCCCCAACCGCCCTAACCCCAGTGCTGGGag                                                                               >  2:864806/1‑196 (MQ=255)
                                                                                                                                                                                                     cagcagcagCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGCAAACGCAGAACCGAAAACCCGCACTGGccca                               >  2:754795/1‑237 (MQ=255)
                                                                                                                                                                                                        cagcagcGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACACCGGCACTAACGCCAGTGCTGGGATTACTGCCCAATCCTGAAACAGCACAACCGCAAACCCGAGCGGGCCgagt                             >  1:593523/1‑235 (MQ=255)
                                                                                                                                                                                                        cagcagcGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCCCTAACGCCAGTGCTGGGATTACTGCCCGATCCTGAAACAGCAGAACCGCAAACCCCGGCTGGCCGGAt                             >  1:672520/1‑238 (MQ=255)
                                                                                                                                                                                                                 cgcgGCGGTAACCCCTTCCCGACCGCAAGAAGCTGCAATAAAGCGGAATGCCGGTCGCAGTAAATAGCTCCCGCCAATCAGCATGCCGACAAACGCCAGCCCCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGGCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGAt                   <  1:78303/239‑1 (MQ=255)
                                                                                                                                                                                                                      gcggTGACACTCTCCCCCCGCCACGAAGCTGCAATAAAGGGGAATCCCGGCAGCAGTAAATGCCGCCCCCCCATCAGCATGCCGACAACCGCCAGCACCTTCATGCCGACCTTACTCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAACCAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCa                <  2:500842/238‑1 (MQ=255)
                                                                                                                                                                                                                            aaCACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGGATACCGGACGCAGTAAATAGCGCCCACCAATCAGCAAGCCGACAAACGCCAGCACCTTCATGCCGACCTTCCTCCAGTCGCAATGGTCGTCTGCCGGACCCCCCCACAACGGCCCTAAACCCCAGTCTGGGGTTACTGCCCACACCCGGAAAAACAGAACCGGAAACCCCACCCGGCCCGACTCCCCCCGAATCAAcccct          >  2:340342/1‑236 (MQ=255)
                                                                                                                                                                                                                                ctccccGCCCCCCTGAAGTTTCAATAAAGCGGAATCCTGGACGGATTAAATTGCGCCCCCCAACCAGCCCGCCGAAAAACGCCAGCGACTTCAGGCCCACCTCCATCCAGTGGAAATGTGCGTCTGCGGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGACCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCa     <  2:380408/236‑1 (MQ=255)
                                                                                                                                                                                                                                 ttCCCGCCCCCCGGAAGCTCCAAAAAAGCGGAAACCCGGACGACATAAATGGCGCCCACCAATCAGCATGCCGACAATCGCCAGCCCCTCCATGCCGACCTTACACCATGCGAAATGTTGGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCAc    <  2:252215/239‑1 (MQ=255)
                                                                                                                                                                                                                                 ttCCCGCAAGCCGAAAGCTCCAAAAAAGCGGAATCCCGGACCCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTCCATGCCGCCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCAc    <  1:877857/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ccccGCACGCCAGAATGCGCAAGAAAGCGGAATACCGGACGCAGTAAATCGCGCCCACCAATCCGCATGCCGACAAACGCCAGCCCCTGCATGCCGACCTTCATACAGTCGAAGTGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACg   <  2:88910/238‑1 (MQ=255)
                                                                                                                                                                                                                                   cccGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTCCGATTCATCCCttttcccccc  >  2:89924/1‑232 (MQ=255)
                                                                                                                                                                                                                                   cccGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCAACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCCCTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCCGAACCGAAAACCCCAGCTGGCCGGATTCCCCCCGATTAATCCCTTTCTCAct   >  2:411344/1‑237 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              
TGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGC  >  NC_000913/3479739‑3480203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: