Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,854 T→G 14.1% V117G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8540TG14.1% 286.9 / 6.3 106V117G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (16/71);  new base G (16/3);  total (32/74)
Fisher's exact test for biased strand distribution p-value = 9.07e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAT  >  NC_000913/429621‑430088
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          
tattatGGGCGGTCGCGGCTTTAACGGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTa                                                                                                                                                                                                                                       <  1:829016/239‑1 (MQ=255)
   tatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGCGGAAACAGGCGGACAGGTGTTGGGGCGCCAGGTTCTGAAGGGGGTTacag                                                                                                                                                                                                                                    >  1:408160/1‑236 (MQ=255)
       ggCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGGGGTGGGCGACCCGGGTCTGGAAGGGGATAAACaaa                                                                                                                                                                                                                                  >  2:280224/1‑238 (MQ=255)
        gCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGGTGGGCGGCCAGGTTCTGAAGGGGGTTAACGaaaac                                                                                                                                                                                                                               >  2:1087967/1‑236 (MQ=255)
         cGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGGGAAACCGGCGGCCAGGGGGTTGGGCGCCAGGGTTTTAAGGGGATTAACAAAccc                                                                                                                                                                                                                               >  2:1069892/1‑238 (MQ=255)
          gTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAATTTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTGTAGGCAGCCGGGTTCTGAAGGGGCTTAACCAATccc                                                                                                                                                                                                                              >  1:292729/1‑237 (MQ=255)
          gTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                                              <  1:300407/238‑1 (MQ=255)
            gcgaGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGCAATCATGGTCCGTATGCCGCCTGTTGAAGGCGAAACCGGCGGCCAGGGGTTGGGGAGCCCGGTTGTAAAGGGTATTACAGAaccaacca                                                                                                                                                                                                                           >  2:150054/1‑239 (MQ=255)
              ccgGCTTTAACGGGGGGCTGGATTTCACCGGTGGTACGTTTATTGAAATTACGGCTGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAt                                                                                                                                                                                                                         <  2:343781/238‑1 (MQ=255)
                   tttAACTGGGGGCTGGATTGCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                                                    <  1:899233/239‑1 (MQ=255)
                     tacgcggggggTGGATTTCGCCGGTGGTTGGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCTGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                                                  <  2:234070/234‑1 (MQ=255)
                     tAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                                                  <  1:212173/239‑1 (MQ=255)
                        cTGGGGGTTGGATTTCCCCGGGGGTACGGTTATTTAAAGTTCCCTCGAAAACCCGGCTGAAATTGAAGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcag                                                                                                                                                                                                               <  2:782466/239‑1 (MQ=255)
                        cTGGGGGCTGGATTTCACCGGTGGTACGGTTAATGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACAGCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcag                                                                                                                                                                                                               <  1:536200/239‑1 (MQ=255)
                             ggttGGTTTTCCCCGGGGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  2:286486/235‑1 (MQ=255)
                             ggCTGGATTTCCCCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  1:808608/238‑1 (MQ=255)
                             ggCTGGATTTCCCCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  1:102958/238‑1 (MQ=255)
                             ggCTGGATTTCACCGGTGGGACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  1:603471/238‑1 (MQ=255)
                               cTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTTAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                                         <  1:168980/238‑1 (MQ=255)
                                    tttCAACGGTGTGACGGTTAGTGAAATTACTCGCGAAAAACCGGCGGAAATTGACGTAATGCGTGATGCATGGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTa                                                                                                                                                                                                   <  1:983154/239‑1 (MQ=255)
                                       cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGTGTTGGGCAGCCCGGGTCTGAAGGTGATTAACGGATCCACCAATCAGAATGAAGCAGGGAAGCGTATTg                                                                                                                                                                                                >  1:1057614/1‑239 (MQ=255)
                                       cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGATCTGACGGGGATTa                                                                                                                                                                                                                                       >  1:1072141/1‑200 (MQ=255)
                                       cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGAGTTGGGCAGCCAGGGTCTGAAGGGGCTTATAGCACCACCCAATCAGAAGACAGCAGCGAAgacgattg                                                                                                                                                                                                >  1:509255/1‑232 (MQ=255)
                                       cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGGGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGTCGGTCCGGTGTTGGGGAGCCAGGTTCTGAAGGGGCGTAACGAATCCACCAATCCGAATGCCGTAGGGaacggatttg                                                                                                                                                                                                >  1:1029138/1‑231 (MQ=255)
                                              tgtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  1:371150/238‑1 (MQ=255)
                                              ggtTCGGTTTTTGAAATTTCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  2:880481/239‑1 (MQ=255)
                                              ggtTCGGTTTTTGAAATTCCCCACGAAAAACCGTGTAAAATTGACGGAAAGCGTGTCGCATTGCAAAAAGCCTGTTTGGAAGAGCCTAGGCTGGAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTCAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  2:176857/239‑1 (MQ=255)
                                               gtACGGTTAGTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGGATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  1:354068/238‑1 (MQ=255)
                                                  cGGTTATTGAAATTACGCTCGAAACGCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTGTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCAGGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGt                                                                                                                                                                                     <  1:1087967/239‑1 (MQ=255)
                                                     ttatgaaaaTTCCGCCCGAAAACCCGGTGGAAATGGACGTAATGGTGGATTCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCg                                                                                                                                                                                  <  2:1077596/233‑1 (MQ=255)
                                                       aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTTAAGGGGGTTAACGAATCCCCCAATCAGAATGCCGCAGGGGAGCGGATTGGGTTCGGCGGGcccga                                                                                                                                                                                >  2:18078/1‑236 (MQ=255)
                                                       aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTAGGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTTGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAAAGCAGCAGTGAAGCGAATTGAAGTCCTCGGGccca                                                                                                                                                                                 >  1:513458/1‑236 (MQ=255)
                                                         ttaatttccgcgcgAAAACCCCGCTGAAATTGACGAAATGCGTGATCATTTGCAAAAGGCCGGTTTGGAGGGGCCGATCCTGCAAAACTTTGGTAGCAGCGATGACAGCAAGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTCCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  2:408160/231‑1 (MQ=255)
                                                         ttaaaTTTCCCTGGAAAAACCGGTTTAAATTGACGTAATGGGGGAGGCATTTAAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  2:749494/237‑1 (MQ=255)
                                                         ttaaaTTCCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  1:1000940/237‑1 (MQ=255)
                                                         tGAAATTCCGCCAAAAAAACCGGCGGAAATTGAGGGAAGTCGGTATGAATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  2:140162/239‑1 (MQ=255)
                                                               tACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCCGAATGCAGCAGTGAAGCGTATTGGGTTCGTCGGTCCGCGCgggggg                                                                                                                                                                         >  1:598081/1‑238 (MQ=255)
                                                                     cGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                                                  <  1:594187/239‑1 (MQ=255)
                                                                            ccGGCTGAAATTGACGTAATGCGTGATGAATTGCAAAAATCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCa                                                                                                                                                           <  1:252054/239‑1 (MQ=255)
                                                                            ccGGCTGAAATTGACGTAAGGCGTGATGCATTGCAAGAAGCAGGTTTGTAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCa                                                                                                                                                           <  1:499070/239‑1 (MQ=255)
                                                                                       ttatgTAATGGGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGATATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGa                                                                                                                                                <  2:513458/235‑1 (MQ=255)
                                                                                         ggttAATGGGGGTTGCATTGGAAAAAGCCGGGTTGGAAGGGCCGATGGTTGAAAACTTTGGGAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                                              <  2:1057614/236‑1 (MQ=255)
                                                                                          cGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGCGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                                              <  1:282941/238‑1 (MQ=255)
                                                                                             atggggttaTGCATTGCAAAAAGCCGGTTTTTAAGGGCCGGTGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCg                                                                                                                                           <  2:504182/230‑1 (MQ=255)
                                                                                                gCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACAAAACCCCCAATCAGAATGCAGCAGGGAAGGGGTTTTGGTTCGTGGGTCCGGGCCGGGGGGCCGCCCTTGGGCAAACCGGGGGGGTGGGGTTgg                                                                                                                                       >  2:1042559/1‑238 (MQ=255)
                                                                                                   tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATg                                                                                                                                     >  1:253310/1‑238 (MQ=255)
                                                                                                    gtggtatggCAAAAAGCGGGTTTGGAGGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGGCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGGTGGGCAGCTAGGTTGTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATgg                                                                                                                                    <  2:619917/230‑1 (MQ=255)
                                                                                                     tttctttGCAAAAAGCCGGTTTTGAAGGGCCGAGGCTTGAAAATTTGGGGAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCa                                                                                                                                  <  2:902026/234‑1 (MQ=255)
                                                                                                      ttcttttaaaaaaGCCGGTTTTGAAGGGCCGATCCGGCAAAACTTTGGTAGCAGCCATGATATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGCTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  2:295436/231‑1 (MQ=255)
                                                                                                      tGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAATCTTTGGTAGCAGCCATGACATCAGGGGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  1:1042559/239‑1 (MQ=255)
                                                                                                      tGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGGCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGATgtgggcg                                                                                                                                  >  1:1001531/1‑232 (MQ=255)
                                                                                                      agCATTACAAAAGGCCGTTTTTGAAGAGCCGATGCTGCAAAACTTTGGTCGCCGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  1:969914/238‑1 (MQ=255)
                                                                                                          tttaaaaaaGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGGAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgct                                                                                                                             <  2:677775/235‑1 (MQ=255)
                                                                                                           ttgaaaaaGCCCGTTTTGAAGGGCCGATTGTGCAAAACTTTGGGAGCGGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:1001531/236‑1 (MQ=255)
                                                                                                           tgaaaaaaGCTGGTTTTGAAGAGCCGATTCTGAAAAACTTTGGTAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:1008275/236‑1 (MQ=255)
                                                                                                           tgaaaaaaGCCGGTGTGGGACAGCCGTTGCTGCAAAACTTTGGTAGCGGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCTGCgctg                                                                                                                            <  2:975733/236‑1 (MQ=255)
                                                                                                           gtaaaacaGCCGTTTTTGAAGGGCGTATGCGGCAAAATTGTGGGAGCCGCGATGACATAGGTGGCCGTAGGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTTTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:33348/236‑1 (MQ=255)
                                                                                                                    ccGTTTTGGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  1:362847/239‑1 (MQ=255)
                                                                                                                     cGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  1:18078/238‑1 (MQ=255)
                                                                                                                     cGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  1:950961/238‑1 (MQ=255)
                                                                                                                       gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGTTGATGGAAGCGCTGCTGGCTTTcccc                                                                                                                >  1:123954/1‑237 (MQ=255)
                                                                                                                       gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                >  1:221041/1‑239 (MQ=255)
                                                                                                                       gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGGCCGGTCGTGGGGGCGAGCCTTGCGCAAACCGGTGCGGTGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                >  1:920161/1‑239 (MQ=255)
                                                                                                                       gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCCGGTTCTTAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTATTGGGTTCGTCGGTCCGCGCGGGGGGGCAGACCCTGCGCAAACCGGTGGGAGGGGGGTGAGGGCGGCGGTGCTGTCTCTcccc                                                                                                                >  2:950961/1‑237 (MQ=255)
                                                                                                                        ttttGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                <  1:845072/238‑1 (MQ=255)
                                                                                                                        ttttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  1:186548/176‑1 (MQ=255)
                                                                                                                        ttttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                <  1:922948/238‑1 (MQ=255)
                                                                                                                                  ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGGTGCTGGCAGAGCTGCGGTCTATCCTCGTGTACGTAgg                                                                                                     >  1:235519/1‑239 (MQ=255)
                                                                                                                                  ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGGTTAACGAATCCCCCAATCAGAATGCAGCAGGGAAGCGGTTTGGGGTCCGCGGTCCTAGCGGGGGGGCAGa                                                                                                                                                                    >  2:186548/1‑176 (MQ=255)
                                                                                                                                                   tGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGAGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGc                                                                                    >  1:540392/1‑239 (MQ=255)
                                                                                                                                                          cgcCTTGACATCAGGGTCCGTATGCCCCCTGCTGAAGGCAAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggc                                                                             <  2:123954/238‑1 (MQ=255)
                                                                                                                                                             cATGACATCGTGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAAGCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                                           <  1:598961/238‑1 (MQ=255)
                                                                                                                                                              aTGACATCAGGGTCCGTATGCCGGCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGTAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                                         <  1:86540/239‑1 (MQ=255)
                                                                                                                                                                   ctcctggggcGTATGCCGCCTGCGGAAGGCGAAACCGGGGGTCAGGGGGGGGGCAGCCAGGGGCGGAAGGTTATGAACGAATCCACCAAGCAGAATGCAGCAGGGAAGCGTATGGAGGGCGGCGGTCCGAGCGGGGGGGCAGACCGGGCGCAAACCGGGGCGAGGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGggt                                                                    <  2:598081/230‑1 (MQ=255)
                                                                                                                                                                   agctttgTCCGGATGCCGCCTGGTGAAGGCGAAACCGGGGGGCAGGTGTTGGGGCGCCGGGTTCTGAAGGGGATTAACGAATCCACCAATGAGAATGCAGCAGGGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgggg                                                                     <  2:292729/232‑1 (MQ=255)
                                                                                                                                                                          tCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGGTGGGCAGCCAGGTTCTTAAGGGGCTTAACCAATTCACCAATCAGAATGCAGCAGTGAAGCGTATTGTGTTCGTCGGACCAGGGGTGGGGGCAGACCTTGCGCAAACCGGGTGGATGGGGTGGATGGCAACGCTGCTGTCTATCCTCGTGTTCTGAGGTTTCCGCTTTGAGTGGGGCCTGGCGGCAGGggtggtagtt                                                             >  2:232291/1‑235 (MQ=255)
                                                                                                                                                                            cGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTTTTg                                                            >  1:908349/1‑238 (MQ=255)
                                                                                                                                                                             gttttccgccTGCCGAAGGCGAAACCGGCGGTTAGGTGTTGGGCAGCCTGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcg                                                          <  2:279719/234‑1 (MQ=255)
                                                                                                                                                                             gTATGCCGGCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcg                                                          <  1:41214/239‑1 (MQ=255)
                                                                                                                                                                               aTGCCGCCTGCTGAAGGCTGAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcgc                                                         <  1:180094/238‑1 (MQ=255)
                                                                                                                                                                                   cgccTTCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcg                                                    <  2:221041/239‑1 (MQ=255)
                                                                                                                                                                                    gccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                                              >  1:438094/1‑164 (MQ=255)
                                                                                                                                                                                    gccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                                              <  2:438094/164‑1 (MQ=255)
                                                                                                                                                                                      cttgtgAAGGGGAAACCGGGGGGTAGGTTTTGGGGCGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCac                                                 <  2:920161/235‑1 (MQ=255)
                                                                                                                                                                                           gAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                                            <  1:324575/239‑1 (MQ=255)
                                                                                                                                                                                           gAAGGCCAACCCGGCGGTCAGGTGTTGGGCAGACAGGTTCTGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                                            <  2:1080635/239‑1 (MQ=255)
                                                                                                                                                                                                       gggggTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATGAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:667440/236‑1 (MQ=255)
                                                                                                                                                                                                       ggcggTCAGGTGTGGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:560468/239‑1 (MQ=255)
                                                                                                                                                                                                       ggcggTCAGGGTTTGGGCTGCCGGGTTCTGGAGGGGATTCACGGATCCCCCAATCAGAATGCAGCAGTGAAGCGTAATGAGTTCGTCGGTTCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:433533/239‑1 (MQ=255)
                                                                                                                                                                                                                  tttggggAAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAGCAGAATGCAGGAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAtttt                       <  2:1059146/234‑1 (MQ=255)
                                                                                                                                                                                                                     tGGGGAGCCGGGTTGTGAAGGTGATTAACGAATCCACCAATCAGAATGCGGCAGTGAACCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATTGCGTTGATGGCAGCGCTGCTGTCTAGCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCg                   <  2:540392/239‑1 (MQ=255)
                                                                                                                                                                                                                     gtgtcAGCCGGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGGTCGGCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGAGGGCAGCGCTGCTGGCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCg                   <  1:25368/235‑1 (MQ=255)
                                                                                                                                                                                                                       ggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCt                                                        >  1:696428/1‑200 (MQ=255)
                                                                                                                                                                                                                       ggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCt                                                        <  2:696428/200‑1 (MQ=255)
                                                                                                                                                                                                                            ccAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGGACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGCGGTTATTGCGCTGGCGCACGACGTTATCATTAGGCGGGGGGTTTTGTGGTGTTTcc            >  2:1003313/1‑239 (MQ=255)
                                                                                                                                                                                                                                 ttCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCatat        <  1:958420/238‑1 (MQ=255)
                                                                                                                                                                                                                                 ttCGGAAGGGTATTAACGAATCCGCCAATCAGAATGCAGCAGGGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGGCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCatat        <  1:476411/238‑1 (MQ=255)
                                                                                                                                                                                                                                   cTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGACCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGGTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCga     >  1:574337/1‑239 (MQ=255)
                                                                                                                                                                                                                                    tGAAGGTTATTAACGAATCCACCAATCAGAATGCAGGAGTGAAGCGTATTGGGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCgag    <  1:1084638/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGCGGGTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAt  >  1:672951/1‑239 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          
TATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAT  >  NC_000913/429621‑430088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: