Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 520,534 A→G 23.7% E40G (GAG→GGG)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913520,5340AG23.7% 98.3 / 4.2 51E40G (GAG→GGG) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (6/31);  new base G (11/2);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 1.90e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCC  >  NC_000913/520298‑520765
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        
tgTTTTCCCTCAACCGTGAACATGGCCCCCGGTTGATTATGGTGACCCACGACCTGCAACGGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGgaga                                                                                                                                                                                                                                        <  1:78032/239‑1 (MQ=255)
      ccccCAAACGTGACCATGGCACCCCGTTGATTATGGTGACCCACCGCCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTTCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCt                                                                                                                                                                                                                                  <  1:602222/239‑1 (MQ=255)
         tCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCCCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGACGGGCttt                                                                                                                                                                                                                                >  1:659607/1‑237 (MQ=255)
          cAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAAAGGGCAGTTGCAGGAGGAAGCATGATGGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATAAATTTTCTGGCTGGCCCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCAAGCACAAGGGGAAGGGCTAAAg                                                                                                                                                                                                                              >  2:145554/1‑239 (MQ=255)
                tGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGGTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGGCTGCGTGCTTGGACTGGGGAAATACAGCGATCGCATGGGGAAGGGGTTAAGCCAgac                                                                                                                                                                                                                        >  2:684134/1‑237 (MQ=255)
                 gAACATGGCACCACGTTGATTATGGTTACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCa                                                                                                                                                                                                                        <  1:768049/238‑1 (MQ=255)
                       ggCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTTTCTGGCTGGCGCTAAGCCTGGCGGGGGCCTGCGTGCTGGGGCTGGGCCATATCCGCGATCGCATGGGGAAGGGCTTAAGCCAGCaaaagcgg                                                                                                                                                                                                                 >  2:96699/1‑234 (MQ=255)
                       ggCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCGCTGGGCAATATCAGGGATCGCATGGCGAAGGGGTTAAGCCCGCAAAGCCGt                                                                                                                                                                                                                 >  1:480769/1‑239 (MQ=255)
                         ccccccGTTGATTATGGTGACCCCCGGCCTGCAACTGGCGGCCCGCTGCGACCGCTGCTTACGGCTGGGGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCg                                                                                                                                                                                                                  <  2:688409/231‑1 (MQ=255)
                              cGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTa                                                                                                                                                                                                          >  1:894890/1‑239 (MQ=255)
                                  gATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATggc                                                                                                                                                                                                      <  1:276603/239‑1 (MQ=255)
                                   aTTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGGGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCCAAGCCGGGAGTTTATg                                                                                                                                                                                                        >  1:688409/1‑236 (MQ=255)
                                   aTTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATTGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATggcg                                                                                                                                                                                                     >  1:851442/1‑239 (MQ=255)
                                   aTTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGc                                                                                                                                                                                                                             <  1:231610/215‑1 (MQ=255)
                                    ttttggtccccccCGCCCTGCAACGGGCGGGCCCCTGCGGCCGCTGCTGACGGGTGGGGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCGGGCGCGAATGGCTTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATggcg                                                                                                                                                                                                     <  2:737008/229‑1 (MQ=255)
                                       tGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGGGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAACCTGGCGGGGGCCGTCGGTGTTGCCCTGGGCAAAATGCGCGATCGCCTGGGGAAGGGGGTAAGCCCGCAAACACGGGGGTTTagtggggggg                                                                                                                                                                                                 >  2:276603/1‑230 (MQ=255)
                                             cccACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTCCGTGCTGGCGCTGGGCACAATACGCGATTCCATGGGGAAGGGCTTTAGCCACCAAAACCCGGGGTTTTTgggggggggggggg                                                                                                                                                                                           >  2:911200/1‑231 (MQ=255)
                                             cccACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGGGGCCTGCGGGCGGGCGCTGGGCAATATCCGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAACCGGGGGTTTATggggggggggcggg                                                                                                                                                                                           >  2:847089/1‑231 (MQ=255)
                                                     cTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTgcg                                                                                                                                                                                    <  1:781401/238‑1 (MQ=255)
                                                                             cGCTTCTTCCGGGTGGGGAACGGGCAGTTGCAGGAGGAAGTATTATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                           <  2:1044867/239‑1 (MQ=255)
                                                                             cGCTGTTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACTTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                           <  2:833376/239‑1 (MQ=255)
                                                                             cGCTGGTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                           <  1:96699/239‑1 (MQ=255)
                                                                               ctgctTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCGCTGGGCAATGTCCGCGAGCGCATGGGGAAGGGCTTAAGCCACACAGGCCGGGGGTTGTTGGCGGGCGGTCGGGGGGGGCGCGGCTGACGCGGAGGGGGGCAAggggg                                                                                                                                                         >  2:1078776/1‑235 (MQ=255)
                                                                                 ggtttgggCTGGTGACGGGGCAGGTGCAGGAGGAGGCTTGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGc                                                                                                                                                       <  2:851442/233‑1 (MQ=255)
                                                                                        gCTGGTGAACGGGCAGTTGGAGGAGGAAGCATGAGTGCACGGTGGTTCTGGCGCGAATGGCGGTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTggagg                                                                                                                                                 <  1:374173/238‑1 (MQ=255)
                                                                                                     cAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGGCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAgcg                                                                                                                                    <  1:263997/238‑1 (MQ=255)
                                                                                                      cgTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAgcgc                                                                                                                                   <  1:939930/237‑1 (MQ=255)
                                                                                                                   aGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGGGATCGGGGGGTGGGCAGGTTACGCGGAAGGCCGCCAGCGTGGCTGGGGGGAAGGCACAAACGCGGCCCGaaaagcg                                                                                                                      >  1:214455/1‑234 (MQ=255)
                                                                                                                      attgttGCCCGTTGGTTCGGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCa                                                                                                                   <  2:894890/234‑1 (MQ=255)
                                                                                                                         aTTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGGGATCGGGGGGGTGGCGGGTCCCCCGAAGGGGCGCAAGCGGGGGTGGGGGAAGGGCAAAAACGCGGGCCGAAAAAAGGCAagag                                                                                                               >  2:133670/1‑237 (MQ=255)
                                                                                                                           tGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                           <  1:705632/193‑1 (MQ=255)
                                                                                                                             cACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACCCGAAGTGCCGCCAGCGTGGCTGGAGGAAGCGCCAAAACGCCGCCCGAAAATCGGCCagcagca                                                                                                            >  1:571963/1‑237 (MQ=255)
                                                                                                                                         ggCGCATATTGCGTTGGCCGTCTCTATTTATTGTCTGGCTGGCGTTTAGCCTGGGGGTGGCCTGGCTTGTGGCGCTGGGCAATTTCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCCCGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCcaca                                                                                              <  2:458471/239‑1 (MQ=255)
                                                                                                                                                          ccGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCCGTTCACACGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAACGCGGCCTGAAAGTCGGCAAGCCACTGACTTTTGCCACACAGACCctttaccgcg                                                                              >  1:254531/1‑231 (MQ=255)
                                                                                                                                                                   ttAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGGTCACGCGAAGTGCCGCAAGCGTGGCTGGGGGAAGCGCAAAAACGCGGCCTGAAAGTCGGCAAGCAGCTGGCTTTTGCCCACAAGACCTTTTCAGGGGACACAccccc                                                                    >  1:788139/1‑236 (MQ=255)
                                                                                                                                                                           ctggctggCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCg                                                             <  1:684134/238‑1 (MQ=255)
                                                                                                                                                                            tggcgggcGCTAAGGCTTGGGGGGGCCTGCGGGCCGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGaa                                                           <  2:214455/239‑1 (MQ=255)
                                                                                                                                                                                           cTGGCGTTGGCCTGCGTGCTGGCGCTGGGCAATGTCATCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGTCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                            <  1:119671/239‑1 (MQ=255)
                                                                                                                                                                                            tGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgat                                           <  1:145554/239‑1 (MQ=255)
                                                                                                                                                                                            gggggggggCCGGCGGGCGGGCGCGGGGAAATAACCGCGCTCGCAGGGGGAGGGGCGTAAGCCAGAAAAGCCGTGAGTTTATGGGGGGCGTTCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgat                                           <  2:991178/232‑1 (MQ=255)
                                                                                                                                                                                                ggTGGCCTGCGTGCTGGCGCTGGGCAATTTCAGCGAGCGCAGGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgatgat                                        <  2:76837/238‑1 (MQ=255)
                                                                                                                                                                                                  tGGCCTGGGTGGTGGCGCTGGGCAATATTAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGatat                                      <  1:335079/238‑1 (MQ=255)
                                                                                                                                                                                                  tGGCCTGCGGGGTGGCGCTGGGCAATTTCAGCAGTCGAATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGatat                                      <  1:692058/238‑1 (MQ=255)
                                                                                                                                                                                                      cTGCGTGCTGGCGCTGGGCAATTTCAGCGATCGGATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAc                                  <  2:453973/238‑1 (MQ=255)
                                                                                                                                                                                                       tGCGTGCTGGCGATGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAcc                                 <  1:178464/238‑1 (MQ=255)
                                                                                                                                                                                                       ggCGTGCTGGCGCTGGGCAATAGCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAcc                                 <  1:302949/237‑1 (MQ=255)
                                                                                                                                                                                                        gggTGCTGGGGCTGGGCAATATCAGCGGTCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCg                               <  2:788139/237‑1 (MQ=255)
                                                                                                                                                                                                                  gcTGGGCAATGTCAGCGATCGCAGGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGc                      <  1:747847/238‑1 (MQ=255)
                                                                                                                                                                                                                          ataaccgCGGTCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCa              <  1:976841/232‑1 (MQ=255)
                                                                                                                                                                                                                           tatCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGGGCCGCAAGCGTGGCTGGAGGAAGCGCACAAGCGCGGCCTGCAAGCCGGCCAACAGCGGAGTTTCGCCCCAATGACCCTTTCAGGCGGCACACCGCCGCTGGGGACCGCCAAAACGGGGGGGGGTATCTTCCCCATGGATGGGGAtgcgacaa            >  2:128659/1‑232 (MQ=255)
                                                                                                                                                                                                                               cggtgtCGCGTGGGGGAGGGCTTAAGCCTGCAAAGACTTGGGTTTATGGCTGGCGGTCGGGCGTTGCGCATTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTaa        <  2:411039/234‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCATGGAGAAGGGCTTAAGCCAGGAAAGCCGGGAGTTTATGGGGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATccccc  <  2:254531/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        
TGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCC  >  NC_000913/520298‑520765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: