Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,305,466 T→C 15.6% E430G (GAG→GGG)  mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,4660TC15.6% 157.2 / 2.4 66E430G (GAG→GGG) mqomalate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (20/33);  new base C (2/10);  total (22/44)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAA  >  NC_000913/2305236‑2305704
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
gTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATc                                                                                                                                                                                                                                           >  1:760703/1‑238 (MQ=255)
                         cAGGTTCCGTCCAAAAGGCGGAACGATCCGTTCCAACTTCGCCCGCCAATGGGGGCCTGAAACACGATCGCCAAATACTGTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGcc                                                                                                                                                                                                                 <  2:581351/239‑1 (MQ=255)
                               gCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGCCGACTTCCGTACCCCACGGCCGGAACCCCCCTTTTTCGGCACCACCCCTTTTAATTCGCACGCGCccccccccc                                                                                                                                                                                                            >  2:609546/1‑235 (MQ=255)
                               gCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCGGAGCGCGGCAATGGTTCCTTGCTGGGCACCGACGACTTCAGAACCCCGACCCCGTACCCCCCCCTTTTCGCCATCCCCCTTGATAATCTGCAACCGCCGccccc                                                                                                                                                                                                              >  2:191730/1‑235 (MQ=255)
                               gCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCCATGGGTCCTTGCTGGTCACTGACGACTTCCGTACCCCGACGCCGGACGCCACCCTTCTTCGCATCACCCCTGTTAATATGAACGCCCGGccccccc                                                                                                                                                                                                            >  2:398020/1‑235 (MQ=255)
                                     aTAAGACGGAACGGTCGTTTTCAACGTGCCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATCCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCcaca                                                                                                                                                                                                    <  2:788593/239‑1 (MQ=255)
                                     aTAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCcaca                                                                                                                                                                                                    <  1:146837/239‑1 (MQ=255)
                                     aTAAGACGGAACGATCCCTTTCAACGTAGCCTGCCTGTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCcaca                                                                                                                                                                                                    <  1:817397/239‑1 (MQ=255)
                                       aaGGCGGAACCATCGCTTTCAACGTAGCCTGCCATTGGGGGCTGGAAACACGATCGCCAAATCCTTTTTCCAGCAGATTCAACATAATCGGCGGGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACaaa                                                                                                                                                                                                  <  2:760703/239‑1 (MQ=255)
                                        aGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACCCGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCcaca                                                                                                                                                                                                    <  1:31189/236‑1 (MQ=255)
                                                        ttaaacggtgCCGGCCATTGCGGGCTGGAAACACGATCGCCAAATTCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCtttttt                                                                                                                                                                                <  1:1062102/230‑1 (MQ=255)
                                                        ttCAACGTGGCCGGCCTTTGCGGGCTGGAAACACGATCGCCAAATACTTTTGCCAGCAGATTCAACAGAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCtttttt                                                                                                                                                                                <  2:575678/239‑1 (MQ=255)
                                                        ttCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCCGGAGCGCGGCAATGGTTCCTTGCTGGTCCCTGACGGCTTCAGTACCCCAACGCAGTAACCCACCCTTTCCCGCCACCCCCCTGATAAACCTCCCCCCCTGCCCCCCCTTCCCCAAACCCCAGACCCTCttttt                                                                                                                                                                                 >  2:230718/1‑239 (MQ=255)
                                                          aaaCGTAGCCTGCCAGTGCGGGCTGGAACCACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCg                                                                                                                                                                              <  1:777071/238‑1 (MQ=255)
                                                             cGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATTCTTTTGCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                                                                                                                           <  1:805140/239‑1 (MQ=255)
                                                             cGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                                                                                                                           <  1:447062/239‑1 (MQ=255)
                                                             cGTAGCCTGCCATGGCGGGCTGGAAACCCGATTGCCAAATTCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCt                                                                                                                                                                           <  1:1022672/239‑1 (MQ=255)
                                                                 gCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCCCCCTTTCCCGCATCACCCTTTGTAAACTTCAACCGCTGCCCCCCTTTCCCCCAAACCCCGCCCCCCTTTTTTCCCTGCgg                                                                                                                                                                       >  2:686165/1‑239 (MQ=255)
                                                                   cTGCCATTGCGGGCTGGAAACCCGATCGCCAAATCCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGAt                                                                                                                                                                     <  1:790914/239‑1 (MQ=255)
                                                                          tGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGTGCGGCCAAGGTTCCTTGCTGGTCAATGGGGACTTCAGTACCCAGGCGCCGTACGCCCCCTTTCTCGGCATCACGCTTGGTAATCTGCACCGCCTGCGCCGCTTGCCCCCAAACCCAGGCCCCTTTTTTCACCAGCGGCTAGGAc                                                                                                                                                                >  2:227862/1‑237 (MQ=255)
                                                                           gCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTTGCGATGCCCCCAGGGCCGCGGCAATGGGTCCTTGCTGGTCACTGACGACTTCCGTACCCAGACGCAGTACGCCCCCCTTCTCGGCCTCACACTTGGTAATCTTCACGCGCTGCCCCCCTTGCCACCAAACCCAGCCCCCCTTTTTTCCCCGGCGGAtgtaccat                                                                                                                                                              >  2:342581/1‑232 (MQ=255)
                                                                                 tGGAAACACGATCGCCAAATCCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTCCGCCTGCGGATAGTACTCTTTCaa                                                                                                                                                        <  1:836952/238‑1 (MQ=255)
                                                                                          gATCGCCAAATGCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCaaaa                                                                                                                                              <  1:191730/239‑1 (MQ=255)
                                                                                             tcccAAGTTCTCTTTCCAGCAGATTCAACATACTCGGCGCGGCGGTTGCCGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACg                                                                                                                                            <  2:210885/236‑1 (MQ=255)
                                                                                             cGCCAAATTCTTTTTCCGGAAGATGCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGTAGCCGGGCAATGGTTCCTTGCTGGTCACTGGCGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACg                                                                                                                                            <  1:246164/238‑1 (MQ=255)
                                                                                              gCCAAATTCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGAt                                                                                                                                          <  1:609546/239‑1 (MQ=255)
                                                                                              gCCAAATTCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGAt                                                                                                                                          <  1:686165/239‑1 (MQ=255)
                                                                                               ccAAATCCTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGc                                                                                                                                                                                                                           <  1:965282/159‑1 (MQ=255)
                                                                                                      cTTTTTCCCAGAAATTCAACCTAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCtt                                                                                                                                    <  1:392255/237‑1 (MQ=255)
                                                                                                       tttttCCAGGAGATTCAAAATAAGCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCAc                                                                                                                                 <  1:703800/239‑1 (MQ=255)
                                                                                                         tttCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTc                                                                                                                               <  1:398020/239‑1 (MQ=255)
                                                                                                           tcccgccggctCAACATTGTCGGCGCGTGGGTTGACGCCCCTGGCGATGCCCCCAGGGCCGCGGCAATGGTTCCTTGCTGGTCCCTGACGGCTTCAGTACCCAGACGCAGTGCGCCCCCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCaa                                                                                                                             <  2:422316/229‑1 (MQ=255)
                                                                                                                         cATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCCTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGa                                                                                                                   <  1:35124/235‑1 (MQ=255)
                                                                                                                                       cggTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGGTTCTTGCTGGGCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACCCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTTGCCTGCGGGTAGGAATCTTTCAACGCCTTAAAACGATCCTCTTCACCCCACAACACCCGCACCACCAGAAAttttaac                                                                                                 >  2:518763/1‑235 (MQ=255)
                                                                                                                                           tgcccccccTGGCCATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGAt                                                                                             <  1:230718/234‑1 (MQ=255)
                                                                                                                                           tGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTTCTGTCTGGTCACCGAAGACGTCAGTTCCCAGACGCAGTACGCCAGCTTGCTCCGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTGTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCTCCTGACTCACCAGATATTTCACCAGAt                                                                                             <  1:498424/239‑1 (MQ=255)
                                                                                                                                                ccccTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTAACCAGATCGaaa                                                                                        >  1:848103/1‑239 (MQ=255)
                                                                                                                                                           gCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAcccc                                                                             >  1:836068/1‑239 (MQ=255)
                                                                                                                                                                      gccgtcAAGGGTTCCTTGCTTGGCCCGGACGACTTCAGTCCCCAGACGCAGTACGCCACCTTTCCCGGCCTCACGCTGTAGAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGcatc                                                                   <  2:782953/233‑1 (MQ=255)
                                                                                                                                                                      cgcgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGTTCCCCTTCACTCAACATCACCTGCCTACCCAGATATTTAACCAGATAAAAATAACCAAGCCCGaacacaacaa                                                                  >  2:424472/1‑230 (MQ=255)
                                                                                                                                                                       ccgagaaTGGTTCCTTCTGTGTCACGGACGACTTCAGTGCCCCAACGCAGTACCCCCCCTTTCTCGGCCGCAGGCGTGTAGATCCTCCCGGCCTGCCCCGCTTGGCACAAACGCCCGGCCTCTTTTGTCGCCTGCGGATATTCCTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGcatcat                                                                 <  2:187109/234‑1 (MQ=255)
                                                                                                                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCAc                                                                                                                                 <  2:248910/174‑1 (MQ=255)
                                                                                                                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCAc                                                                                                                                 >  1:248910/1‑174 (MQ=255)
                                                                                                                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCatcatc                                                                >  1:782953/1‑239 (MQ=255)
                                                                                                                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCatcatc                                                                >  1:60827/1‑239 (MQ=255)
                                                                                                                                                                        cgGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAACCCCACGCGcatccac                                                                >  2:207765/1‑236 (MQ=255)
                                                                                                                                                                             atttttCCTTGCTGGTCCCTGACGACTTCAGGACCCAGACCCAGTACGCCCCCTTTCCCGGCATCCCGGCTGATAATCTGCCCCCGCTGCCCGGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  2:309433/235‑1 (MQ=255)
                                                                                                                                                                             aTGGTTCCTTTCTGGTCACTAACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  1:907680/239‑1 (MQ=255)
                                                                                                                                                                             aTGGTTCCTTGCTGGTCCCGGGCGCCTTCAGGACCCAGACGCAGTACGCCCCCTTTCCCGGCATCCCGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  2:745123/239‑1 (MQ=255)
                                                                                                                                                                             aTGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  1:207765/239‑1 (MQ=255)
                                                                                                                                                                             aTGGTTCCTTGCTGGTCACGGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  1:627218/239‑1 (MQ=255)
                                                                                                                                                                             aTGGTTCCTTGCGGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCGCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  1:518763/239‑1 (MQ=255)
                                                                                                                                                                             aTGGTTCCTTGCCGGTCCCTGACGACTTCAGTGCCCGGACGCCGTACGCCCCCTTTCCCGGCATCACGCTTGATAATCTGCACGCCCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  1:109433/239‑1 (MQ=255)
                                                                                                                                                                              tGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATc                                                          >  1:99369/1‑239 (MQ=255)
                                                                                                                                                                                 gtttctttgttgTCCCTGACCATTTCCATTCCCAGACGCCGTCCCCCCCCTTTCCCGGCATCCCGGTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCAt                                                           <  2:60827/225‑1 (MQ=255)
                                                                                                                                                                                   ccTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCCCCAGATCGAAATTATCCACCCCGCCGTGCATCATCAGCCTCACGtt                                                     >  2:1030636/1‑239 (MQ=255)
                                                                                                                                                                                         tGGTCCCTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGGCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAggt                                               <  1:544498/239‑1 (MQ=255)
                                                                                                                                                                                          ggTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAggtg                                              >  1:45494/1‑239 (MQ=255)
                                                                                                                                                                                            gTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCCACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGGGCATCACCGGCATCACGTTAGAggggg                                             >  2:406756/1‑239 (MQ=255)
                                                                                                                                                                                                     ggcTTCAGCACCCAGCCGTGTTACGCCACCTTTCCCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCCCCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGaa                                     <  1:227862/236‑1 (MQ=255)
                                                                                                                                                                                                      acTTCAGTACCCAGACGCAGTACGCCACCTTTCCTGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACt                                   <  1:342581/239‑1 (MQ=255)
                                                                                                                                                                                                        ttCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATc                                                                                                                         <  1:317648/151‑1 (MQ=255)
                                                                                                                                                                                                        ttCAGGACCCAGACGCAGTACGCCTCCTTTCCTGGCATCACGCTTGATAAGCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCa                                 <  1:943920/239‑1 (MQ=255)
                                                                                                                                                                                                                  aGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTTCATCATCGGCATCACGTTAGAGGTGGTGGTGGCACTCATTAGAtacac                       >  2:873130/1‑235 (MQ=255)
                                                                                                                                                                                                                            cGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCg             >  1:676788/1‑239 (MQ=255)
                                                                                                                                                                                                                                     ttCTCGGCATCACGCTTGTTAATTTGCCCGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAg     <  2:836068/239‑1 (MQ=255)
                                                                                                                                                                                                                                        tcGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGGGGGGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGaa  >  1:80581/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
GTTCTGTTGCCGCTACATCACCGTTCAGCTTGCGTCCATAAGACGGAACGATCGCTTTCAACGTAGCCTGCCATTGCGGGCTGGAAACACGATCGCCAAATACTTTTTCCAGCAGATTCAACATAATCGGCGCGGCGGTTGACGCCCCTGGCGATGCCCCCAGGAGCGCGGCAATGGTTCCTTGCTGGTCACTGACGACTTCAGTACCCAGACGCAGTACGCCACCTTTCTCGGCATCACGCTTGATAATCTGCACGCGCTGCCCCGCTTGCCACAAACGCCAGTCCTCTTTTTTCGCCTGCGGATAGTACTCTTTCAACGCTTCAAAACGATCCTCTTCACTCAACATCACCTGACTCACCAGATATTTCACCAGATCGAAATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAA  >  NC_000913/2305236‑2305704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: