Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,153,595 A→C 7.7% intergenic (‑497/+101) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,153,5950AC 7.7% 189.6 / 9.5 65intergenic (‑497/+101)ppc/argEphosphoenolpyruvate carboxylase/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base C (3/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

AAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCTTCGGTACAGTAGTTCACCACTTCGGTTTTT  >  NC_000913/4153311‑4153879
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             
aaaTGTTAAAAAATCGCCCCAAGGAACACAAAAGGTGTGGGTCGGATAAGATGCGAAAGTAGCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGGTGGGCATCGTTTGAGACACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGt                                                                                                                                                                                                                                                                                            <  2:400254/288‑1 (MQ=255)
    gTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGAt                                                                                                                                                                                                                                                                                       >  1:388860/1‑289 (MQ=255)
       aaaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAg                                                                                                                                                                                                                                                                                    <  2:388860/289‑1 (MQ=255)
                  caaaGTAAAACCACAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGCCATGATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAgca                                                                                                                                                                                                                                                                         <  2:522112/287‑1 (MQ=255)
                        aaCACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAgcatcgca                                                                                                                                                                                                                                                                    <  1:276943/288‑1 (MQ=255)
                           acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCGTCGTTCGAGTCACTGTAACTCGGATACGATGCGCAAGCATCTCAACCg                                                                                                                                                                                                                                                                >  2:416273/1‑289 (MQ=255)
                                             aTAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTTAGTCACTGTAAGTCGGCTAAGATGCGCAAGCATCGCATCCGACCTTATTGCGGCACTgg                                                                                                                                                                                                                                              >  2:388843/1‑289 (MQ=255)
                                                       cAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCAGTGTAGGTCCGATAAGATTCGTAAGCATCGCATCCGGCATTATTGGGGCACTGGAATTTggta                                                                                                                                                                                                                                     >  2:290469/1‑286 (MQ=255)
                                                                          gattGCGGCACGGGAGTTTGGCAACGGTGCCGGAGGGGGCGCGTGCGCCTTATCCGGCGTAAAGTTGGGAATCGGTTGAGTCGCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGcgcg                                                                                                                                                                                                                 <  2:136804/288‑1 (MQ=17)
                                                                                 gCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGCTGCGgcgccgcggcct                                                                                                                                                                                                          >  2:153272/1‑281 (MQ=255)
                                                                                 gCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGACTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGAGAAGATGCGCCAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCCACAGGGCCGGATGCGGCTCGAGCGCCt                                                                                                                                                                                                          >  2:211905/1‑289 (MQ=255)
                                                                                     gggAGTTTGGCAACAGTGCCGAATGGGGCGCGGGCGCCTTATCCGGCCTAAAGTTGGGCATCGTTTGGGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                                                                                      <  2:408801/288‑1 (MQ=255)
                                                                                            tGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCCTCGTTTGAGTCACTGTCGGCCGGATAAGATGCGCAAGTATCGCATCCGTCATTATTGCCGCACTGGAGTTTGGCGACAGTGCCGGATGCGGTGCGAGCGCCTTCTCCGGCCt                                                                                                                                                                                                >  2:290329/1‑288 (MQ=17)
                                                                                                    gTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGGTGCGGCGCGAGCGCCTTAGCCGGCCTTCGCTTTaa                                                                                                                                                                                       >  2:41991/1‑289 (MQ=255)
                                                                                                                ggcggAGGGCCTTATCCGGCCTACGTTTGGGAATCGTTTGAGTACTGGTAGGTCGGATAAGATGGGCAAGTATCGATTCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCGGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCaaaa                                                                                                                                                                            <  1:388843/284‑1 (MQ=17)
                                                                                                                 cgcgAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACCGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGACTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCCAGCATCGCATCCGACATTATTGCCGCACTGGCGCTTGGCAACAGCCCCGGACGCGGCGCGAGCCCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAAt                                                                                                                                                                          >  1:96677/1‑289 (MQ=255)
                                                                                                                      gcgcCTGTTCCGGCCAACAGTTGGCCATGTTTTGAGTCACTGTCGGTCGGTTAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTg                                                                                                                                                                      <  1:66950/288‑1 (MQ=255)
                                                                                                                       ctccTTATCCGGCTTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGa                                                                                                                                                                     <  1:60205/286‑1 (MQ=255)
                                                                                                                            tatcagagttaaaGTTGGGCATCGTTTGAGTCATTGTCGGTCGGTTAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTAcc                                                                                                                                                               <  2:500708/277‑1 (MQ=255)
                                                                                                                            tATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGGCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGAAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTAcc                                                                                                                                                               <  1:153272/289‑1 (MQ=255)
                                                                                                                                    ttACGGTTGGGCATCGTTTGAGTCACTGTGGGTCGGATAAGATGCGCAAGTATCGCATCCGAAATTATTGCGGCACTGGGGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTAt                                                                                                                                                       <  1:290329/288‑1 (MQ=255)
                                                                                                                                             ggCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCCCTGGAGTTTGGCAACAGCGCCGGCTGCGGGGCGAGCCCCTTATCCGGCCTACGTTTTACTGCCACCAAAAATGGTGAATTAACTGGGTTATCAGttaggg                                                                                                                                              >  1:399955/1‑285 (MQ=255)
                                                                                                                                                 tCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAgcatcg                                                                                                                                                                                                                                                                      <  2:453330/165‑1 (MQ=255)
                                                                                                                                                 tCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAgcatcg                                                                                                                                                                                                                                                                      >  1:453330/1‑165 (MQ=255)
                                                                                                                                                    tttGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGCGTCCCTGTAGGTCGGATAAGATGCGCCAGCATCGCATCCGACATTATTGCGGCCCTGGAGATAGGCCACCGCGCCGCATGGGGCGCGAGCCCCTTATCCGGCATACCTTTTACGGCCAGCAAAAATGGTGAATTACCTGGGTCTTCAGGTCGCCGGGTg                                                                                                                                         >  2:415790/1‑287 (MQ=17)
                                                                                                                                                       gAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGCTGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTACTGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGTCGGTTGGCTTg                                                                                                                                   >  1:167810/1‑289 (MQ=255)
                                                                                                                                                          aaggtGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATa                                                                                                                                >  2:111795/5‑289 (MQ=255)
                                                                                                                                                              tGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATgcgc                                                                                                                                                                                                                                                                              <  2:206910/144‑1 (MQ=37)
                                                                                                                                                              tGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCCACCGCGCCGGATGCGGCGCGCGCGCCCTATCCGGGCGACGTTTTAATTCCAGCACAAATGGTTAATTATCCGGGTTATCAGTTTGCGCGTGGGCTTgttcaat                                                                                                                             >  2:429295/1‑282 (MQ=17)
                                                                                                                                                                    gtcggAAAAGATGCGCATGTATCGCATCCGACAGTATTGCGGAACTGGAGTGTGGCAACAGTGCCGGAGGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCgtgt                                                                                                                        <  1:186084/288‑1 (MQ=255)
                                                                                                                                                                       ggATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTcc                                                                                                                    <  1:111795/289‑1 (MQ=255)
                                                                                                                                                                       ggATAAGATGCGAAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTcc                                                                                                                    <  1:290469/289‑1 (MQ=255)
                                                                                                                                                                         aTAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAgcatcgca                                                                                                                                                                                                                                                                    >  1:206910/1‑144 (MQ=37)
                                                                                                                                                                           aaGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGat                                                                                                                <  1:68157/289‑1 (MQ=255)
                                                                                                                                                                                   cAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGGCTTTATTTCGGCCCCGGAGTTTGGCAACAGCGCTGGATGCGGCGTCAGCGCCTTATCCGGCCTACGTTTTAATGCCCTCAACAATGGATGCTTACCTGGGTTATCCATTTGCGGGTTAGCTTGTCACACCGTGCTTCCAAATATtcatca                                                                                                        >  2:297357/1‑289 (MQ=14)
                                                                                                                                                                                   cAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTTAATTACCTGGTTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATtcatcc                                                                                                        >  2:81144/1‑288 (MQ=255)
                                                                                                                                                                                   aaagcagcGCATCCGACTATATGCCGTCACTGGAGTTTGGCAACAATGCCGGATGCGGCGCGAGCGCCATAGCCGGCCTACGGTTGGGCATCTGTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATtcatc                                                                                                         <  2:302675/281‑1 (MQ=21)
                                                                                                                                                                                       caTCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                                                                                      <  2:270842/191‑1 (MQ=37)
                                                                                                                                                                                              tcggaaattgttGCGGCACGGGAGTTTGGCAACAGGGCCGGAGGCGGCGCGAGTGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCAACGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGc                                                                                             <  2:96677/283‑1 (MQ=255)
                                                                                                                                                                                              tCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGGTGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAg                                                                                              >  1:460659/1‑288 (MQ=255)
                                                                                                                                                                                              tCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTAcc                                                                                                                                                               >  1:190252/1‑224 (MQ=255)
                                                                                                                                                                                              tCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTAcc                                                                                                                                                               <  2:190252/224‑1 (MQ=255)
                                                                                                                                                                                                 gACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGt                                                                                                                                                                                            >  1:270842/1‑192 (MQ=37)
                                                                                                                                                                                                   cATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGa                                                                                         <  1:41991/288‑1 (MQ=255)
                                                                                                                                                                                                       atggCGGAACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCAAGCGCCTTATCCGGCTTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGAATAAGATGCGCAAGCATCGCATCCGAAATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAAt                                                                                    <  1:464772/286‑1 (MQ=255)
                                                                                                                                                                                                           cGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGACGCGGCGAGAGAGCCTTATCCGGCCTACGTTTTATTGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAGACCGTGCTTCCCGATATTCATTAGGTTGATGATCCTGATGTATTGa                                                                                 >  1:416855/1‑288 (MQ=21)
                                                                                                                                                                                                             gCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTAt                                                                                                                                                       >  1:15327/1‑217 (MQ=255)
                                                                                                                                                                                                             gCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTAt                                                                                                                                                       <  2:15327/217‑1 (MQ=255)
                                                                                                                                                                                                             gCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGc                                                                               >  2:101517/1‑288 (MQ=255)
                                                                                                                                                                                                                     gTTTGGAAACAGGGCCGGATGCGGGGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCAGCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGcccc                                                                       <  1:416273/288‑1 (MQ=255)
                                                                                                                                                                                                                             aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGc                                                                                             >  2:378421/1‑258 (MQ=255)
                                                                                                                                                                                                                             aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGc                                                                                             <  1:378421/258‑1 (MQ=255)
                                                                                                                                                                                                                               aaggCCGGGTGCGGAGCGAGCGCCGTAGCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATGATTGCGGAACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGt                                                            <  2:169875/286‑1 (MQ=255)
                                                                                                                                                                                                                                    cGGATGGGGCGCGAGCGCCTTAGCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGGGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCggg                                                        <  1:211905/288‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACg                                     >  2:427793/1‑288 (MQ=255)
                                                                                                                                                                                                                                                            cgtccTACGGTTAGGATGCGTTTGAGTCACGGTGGGTCGGATAAGATGCGCAAGCATTGGATCCGACATTCTTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGAGCCTTATCCGGCCTACGTTCTAATGCCAGCAAAAATGGTGAATTACCTGGTTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCtt                               <  2:115986/286‑1 (MQ=255)
                                                                                                                                                                                                                                                            cGGCCTACGGTTGGGCATCGTTTGATGCACTGTAGGTCGGATAAGATGCGAAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCtt                               <  1:414359/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              gCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGAATAACGTTTGAATAAACGGCGCTTCg                             >  1:16851/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        gggCATCGTTTGAGTCACTGTACGACGGATAATATGCGCAAGCATCGCATCCGACATCATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGACTTATCCGGCCTACGCTTTAATTTCCGCTCAACTGGCGAATTATCTGGGCTATCAGTTCGCGGTTGGTCTTGATAAACCGTGTTTTCAGATATTCATCACGTTGATTAGGCTGATTATTTGAGACAGGCCCCAACACCATCGACGCACATAACCTTTGAATATCAGGCGCTTCAGTACagtcgt                   >  2:74026/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         ggCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCCCTTCGGTACagtagt                   >  2:237510/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                             tCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCTTCGGTACAGTAGTTcacc              >  1:288983/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              cGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCTTCGGTACAGTAGTTcacc              >  2:468938/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                   gAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGt                                                            >  2:355032/1‑237 (MQ=255)
                                                                                                                                                                                                                                                                                   gAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGt                                                            <  1:355032/237‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          tGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCTTCGGTACAGTAGTTCACCACTTCGGttttt  >  1:465890/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             
AAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTAATGCCAGCAAAAATGGTGAATTACCTGGGTTATCAGTTCGCGGGTGGGCTTGATAAACCGTGTTTCCAGATATTCATCAGGTTGATGAGCCTGATTAATTGAGCCAGGCCCCAACACCAGCGTCGGGCATAACGTTTGAATAAACGGCGCTTCGGTACAGTAGTTCACCACTTCGGTTTTT  >  NC_000913/4153311‑4153879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: