Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,213,570 Δ48 bp coding (524‑571/1842 nt) rpoD → RNA polymerase, sigma 70 (sigma D) factor

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3213570 3213617 48 51 [0] [0] 51 rpoD RNA polymerase, sigma 70 (sigma D) factor

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 32135690 (0.000)47 (0.760) 41/264 0.5 100% coding (523/1842 nt) rpoD RNA polymerase, sigma 70 (sigma D) factor
?NC_000913 3213618 = 0 (0.000)coding (572/1842 nt) rpoD RNA polymerase, sigma 70 (sigma D) factor

CTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGTGACACCATCAAAGCGA  >  NC_000913/3213476‑3213758
                                                                                                                                              |                                                                                                                                            
aaccaggttcaatgctccgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagccgaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaaggcagaagaagagctgga                                                                                                                                              >  2:556915‑M2/143‑143 (MQ=255)
aaccaggttcaatgctccgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctgga                                                                                                                                              >  1:896674‑M2/143‑143 (MQ=255)
 accaggttcaatgctccgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggAc                                                                                                                                             >  2:459399‑M2/142‑143 (MQ=255)
    aggttcagtgcgccgttgatggatgaccggaagcgatcacctagctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGAt                                                                                                                                          <  1:493687‑M2/5‑1 (MQ=255)
     ggttcaatgctccgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgactcgaacgcagaaggagttctggTCGATg                                                                                                                                         >  1:640548‑M2/138‑143 (MQ=255)
      gttcaatgctccgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagcagcgcgtcggtccgatctgatcaccggcttggtcgacccgaacgcagcagaagaacgggACCATGa                                                                                                                                        >  2:995723‑M2/137‑143 (MQ=255)
      gttcaatgctccggtgctgaatatccggaagcgagcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGa                                                                                                                                        <  1:1087137‑M2/7‑1 (MQ=255)
            tgctccgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGa                                                                                                                                  >  2:314446‑M2/131‑143 (MQ=255)
              ctccgttgctgaatatccggacgcgattacctatctgccggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATg                                                                                                                                <  1:320789‑M2/15‑1 (MQ=255)
                 cgttgctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtcggcaccatcttatctccgtttttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAg                                                                                                                             <  1:555112‑M2/18‑1 (MQ=255)
                  gctgctgaatatccgggagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGa                                                                                                                            <  2:58575‑M2/19‑1 (MQ=255)
                      ctgaatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagacgaagatctggACGATGACGAAGATGAAGACgaa                                                                                                                        >  2:715382‑M2/121‑143 (MQ=255)
                         aatatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACgaagaa                                                                                                                     >  1:801894‑M2/118‑143 (MQ=255)
                          atatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgcctgtccgatctgatcaccggctacgttgacccgaacgcagaagaagatctggACGATGACGAAAATGAAAACGaagaag                                                                                                                    >  2:911483‑M2/117‑143 (MQ=255)
                           tatccggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggcattgttggcccgaacgccgaagaagatctcgACGATCACGAAGAAGAAGACCAcgaaga                                                                                                                   >  2:815376‑M2/116‑143 (MQ=255)
                              ccggaagcgatcacctatctgcgggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgagctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATgg                                                                                                                <  2:942787‑M2/31‑1 (MQ=255)
                                ggaagcgatcacctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCg                                                                                                              >  2:102486‑M2/111‑143 (MQ=255)
                                     cgatcacctatctgctggaacaggacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATgac                                                                                                         <  1:631142‑M2/38‑1 (MQ=255)
                                            ctatctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAgcg                                                                                                  >  1:849265‑M2/99‑143 (MQ=255)
                                               tctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGGTGGCGAGGACGACAGCGCCg                                                                                               >  2:5786‑M2/96‑143 (MQ=255)
                                               tctgctggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggAACGTGTCTTAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCg                                                                                               >  1:868433‑M2/96‑143 (MQ=255)
                                                gtgctggagcagtacgatcgtgttgatgcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGTCGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCga                                                                                              <  2:988201‑M2/49‑1 (MQ=255)
                                                    tggacccgtagggtcgtgttgcagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCgatgat                                                                                          <  2:318385‑M2/53‑1 (MQ=255)
                                                    tggaacagtacgatcgggttgaagcagaagaagggcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCgatgat                                                                                          <  2:102107‑M2/53‑1 (MQ=255)
                                                     ggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGatgatg                                                                                         >  1:1111607‑M2/90‑143 (MQ=255)
                                                     ggaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcacaggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGatgctg                                                                                         >  1:626054‑M2/90‑143 (MQ=255)
                                                      gaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGAtgatga                                                                                        <  1:941685‑M2/55‑1 (MQ=255)
                                                      gaacagtacgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgtggacccgaacgcagaggaagatctggACGCTGACGAGCATGGAGACGAAGACAATGGCGAGGACGACAgcgcggatagtga                                                                                        >  2:92092‑M2/89‑138 (MQ=255)
                                                              cgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGCAGAAGATGGCGCTGGCGACAGCGCCGATGATGACAACAGCa                                                                                >  1:1151408‑M2/81‑143 (MQ=255)
                                                              cgatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcacaggctttgttgacccgaacgcagaagacgacctggACGAAGACGACGATGACGACGAACCAGATGGCGACGACGACCGCGCCGACGACGACAACAGCa                                                                                >  2:455329‑M2/81‑143 (MQ=255)
                                                               gatcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCAt                                                                               <  1:1174327‑M2/64‑1 (MQ=255)
                                                                 tcgtgttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCg                                                                             >  2:191596‑M2/78‑143 (MQ=255)
                                                                  cgggttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGa                                                                            <  1:370692‑M2/67‑1 (MQ=255)
                                                                     gttgaagcagaagaagcgcgtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGAccc                                                                         >  1:158037‑M2/74‑143 (MQ=255)
                                                                                agaagcgcgtctgtccgatgtgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTc                                                              <  2:874619‑M2/81‑1 (MQ=255)
                                                                                      gcgtgtgtccgatcggatcacgggctttggggacccgaacgcggaagaagatctggACGATGACGAAGATGAAGACGAAGAGGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGTCCCGGAACTGGCTCGCGaaa                                                        <  2:148633‑M2/87‑1 (MQ=255)
                                                                                        gtctgtccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACCGCGCCGATGATGCCAACAGCCTCGACCCGGAACGGGCTCGCGaacaa                                                      >  2:238540‑M2/55‑143 (MQ=255)
                                                                                              ccgatctgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCg                                                <  1:459399‑M2/95‑1 (MQ=255)
                                                                                                   ctgatcacgggctttgtggatccgaacgcagaagaagagctggAGGATGACGAAGATGAAGACGAAGAAGAGGGCAATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACt                                           <  1:500614‑M2/100‑1 (MQ=255)
                                                                                                    tgatcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTa                                          >  2:171353‑M2/43‑143 (MQ=255)
                                                                                                      atcaccggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGGAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAAGTGGCTCGCGAAAAATTTGCGGAACTAcg                                        >  2:672976‑M2/41‑143 (MQ=255)
                                                                                                           gggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTc                                   <  1:1033498‑M2/108‑1 (MQ=255)
                                                                                                            ggctttgttgacccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGAGCCGGAACTGGCTCGCGAACAATTTGCGGAACTACGCGCtcc                                  >  1:408869‑M2/35‑142 (MQ=255)
                                                                                                                      acccgaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTa                        >  2:497803‑M2/25‑143 (MQ=255)
                                                                                                                          taacgcagaagaatatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAAcgc                    <  1:1070243‑M2/123‑1 (MQ=255)
                                                                                                                          gaacgcagaagaagatctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCCCGCGAAAACTTTGCGGAACTACGCGCTCAGCACGGTGTAAcgc                    >  2:52352‑M2/21‑143 (MQ=255)
                                                                                                                            acgcagaagaagacctggACGAGGACGAAGACGAAGACGAAGAGGATGGCAATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGt                  <  2:510549‑M2/125‑1 (MQ=255)
                                                                                                                                     agtatcttgGCGATGACGAAGATGAAGACGAAGAAGAGGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGTGACACCATc         <  2:338101‑M2/134‑1 (MQ=255)
                                                                                                                                        atctggACGATGACGAAGACGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGTGACACCATCaaa      <  1:53114‑M2/137‑1 (MQ=255)
                                                                                                                                        acctggACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGTGACACCATCaaa      <  1:123797‑M2/137‑1 (MQ=255)
                                                                                                                                            ggACGATGACGAAGATGAAGCCGAAAAAGATGGCGTTGACGACAGCGGCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGTGACACCATCAAAGCGa  <  2:1010059‑M2/141‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
CTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGTAACGCGTGACACCATCAAAGCGA  >  NC_000913/3213476‑3213758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.