New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1691381	55 (0.860)	3 (0.050)	3/260	9.6	5.8%	intergenic (+21/+205)	ydgA/uidC	DUF945 family protein/putative outer membrane porin for beta‑glucuronides porin protein
	?	NC_000913	= 1691389	47 (0.800)					intergenic (+29/+197)	ydgA/uidC	DUF945 family protein/putative outer membrane porin for beta‑glucuronides porin protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAC  >  NC_000913/1691249‑1691530                                                                                                                                             |                                                                                                                                              tAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCGATTACGCAGGAGTAATTGAGGGACAAAAAGACGGAGAATTTTGACt                                                                                                                                             >  2:1036493/1‑143 (MQ=255)    gggTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCg                                                                                                                                          <  2:923014/142‑1 (MQ=255)       gcAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCg                                                                                                                                       <  2:357679/142‑1 (MQ=255)           aTAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGtttt                                                                                                                                   >  1:802440/1‑143 (MQ=255)           aTAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGtttt                                                                                                                                   >  1:518275/1‑143 (MQ=255)            tAACGTTAAATGGGCAGCAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCACTTAACGTTCCGGCTGTACATGCAATTCCGCAGAAGTCACTGGCGGCCAAAACGCCGGAGAATTTTCACTCCGGCGctttg                                                                                                                                  >  2:239765/1‑142 (MQ=255)            tAACGTTAAATGGGCAGAAAATGTCGTTATAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTc                                                                                                                                  <  1:704124/143‑1 (MQ=255)             aaCGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAGTTTTCACTCCGGCGTTTGCa                                                                                                                                 >  2:29550/1‑143 (MQ=255)              aCGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCAt                                                                                                                                <  2:541630/143‑1 (MQ=255)              aCGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTCTTcca                                                                                                                                >  2:304027/1‑141 (MQ=255)               cGTTAAGTGGGTCGAAAATGTCGTTAGAAGATTTTGTTCGCATGTTTGCGATGCCAGCTCTTAACTCTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATc                                                                                                                               <  2:653651/143‑1 (MQ=255)                gTTAAATCGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCt                                                                                                                              <  1:339528/143‑1 (MQ=255)                      tGGGCAGAAAATGTCGTTAGAAGATTTTGGTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaac                                                                                                                        <  1:540074/143‑1 (MQ=255)                         gCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCAAGGCCAAAACCCCCGAGAATTTTCACCCACGCGATTTCATCTGAaaacaaa                                                                                                                     >  2:1056603/1‑143 (MQ=255)                          cAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAaaacaaac                                                                                                                    >  2:598053/1‑143 (MQ=255)                                  gTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGAtt                                                                                                            <  2:827440/143‑1 (MQ=255)                                   tCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAACACAAACCAGAGATTg                                                                                                           >  1:740268/1‑143 (MQ=255)                                    cGTTAGAAGATTTTGCTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGc                                                                                                          <  1:553065/143‑1 (MQ=255)                                      ttAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCGATTCCGGAGCGGTGTTTCAGGGCCGAATCGCCGGAGGATTTTCACTCCGGCGCTTTCAGCTGAAAACAAACCAGAGATTACCt                                                                                                        >  1:295430/1‑143 (MQ=255)                                       tAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTg                                                                                                       <  1:1134239/143‑1 (MQ=255)                                         gaagaTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGAt                                                                                                     <  1:753430/143‑1 (MQ=255)                                            gaTTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCCGCAATTCACGGCCAAAACGCCGGAGACTCATCACTCCAGCGTATTACTCTGACAACAAACCAGAGATTGCCTGATgcg                                                                                                  >  2:399285/1‑143 (MQ=255)                                                    tgGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCtt                                                                                          >  2:661412/1‑143 (MQ=255)                                                     ggCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCCAAACGCCGGAGAATTTTCACTCCGGCCTTTTCATCCGACAACAACCCACAGATCCCCTGATGCGCTACGcctc                                                                                         >  1:81710/1‑140 (MQ=255)                                                        aTGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCa                                                                                      >  1:107486/1‑143 (MQ=255)                                                         tGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGCAAACACACCAGAGATCGCCTGCTCAGCGACGCtgagcca                                                                                     >  2:744449/1‑137 (MQ=255)                                                           tttGCGATGCCAGCTCTTAACGTTCCGGCTGTACTGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGACAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGc                                                                                   >  1:606418/1‑143 (MQ=255)                                                           tttGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGc                                                                                   <  2:537417/143‑1 (MQ=255)                                                           tttGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGc                                                                                   <  1:60270/143‑1 (MQ=255)                                                                gATGCCAGCTCTTGACGTTGCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTGTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTAcc                                                                              <  1:757776/143‑1 (MQ=255)                                                                   gCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCttt                                                                           >  1:995143/1‑143 (MQ=255)                                                                      aGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCACAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAAACAAAGAATGCCTGACGCGCTACCCTTATCAGGCCTAGCTTATcc                                                                        >  2:109540/1‑143 (MQ=255)                                                                        ctctTAACGTTCCGGCTGGACCGGCAAGTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTg                                                                      <  1:236680/143‑1 (MQ=255)                                                                           ttAACGTTCCCGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCACAACGCCGGAGAATTTTCACTCCCTTGTTTTCATCTGGCAACAATCCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCaa                                                                   <  2:245546/143‑1 (MQ=38)                                                                            tAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCCCCAac                                                                  >  2:434138/1‑143 (MQ=255)                                                                                gTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAGGGGGCCCAAAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACtt                                                              >  1:843899/1‑143 (MQ=255)                                                                                 ttCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACttt                                                             <  1:598053/143‑1 (MQ=255)                                                                                 tgccGGCTGTACCGGCAAGTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACttt                                                             <  2:72804/141‑1 (MQ=255)                                                                                       cTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGTGTTTTCATTTGAAGACAAACCGGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAAttt                                                       <  1:53655/143‑1 (MQ=255)                                                                                           aCCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGa                                                   <  1:681633/143‑1 (MQ=255)                                                                                              ggCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGtt                                                >  2:547121/1‑143 (MQ=255)                                                                                                 aaTTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGttttt                                             >  2:696629/1‑143 (MQ=255)                                                                                                         agcagTGATTCACGGCCAAAACGCCGGAGATTTTGCCCGCCGGCGTTTGCATCTGAAAACAAACCAGAGATTGCCTGATGCGATACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTg                                     <  1:790576/143‑1 (MQ=255)                                                                                                           cagTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGa                                   <  2:999856/143‑1 (MQ=255)                                                                                                            agtagtTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACCTCCCATTTATGAGTTTTTGTAGGCTGGAt                                  <  2:142562/143‑1 (MQ=39)                                                                                                                    aCGTCCAAAACGACGGAGAATTTTCACTCCGGCGTCTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGtt                          <  2:136990/143‑1 (MQ=255)                                                                                                                    aCGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACGAACCAGAGATTGCCTGATGCGCTAGGCTTATCAGGCGTACCTTTTCCAGGAACACTATGAAGCTATGAGTTATTGTAGGCTGGATGGGGCGtt                          >  1:154630/1‑143 (MQ=255)                                                                                                                    aCGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGtt                          >  1:290811/1‑143 (MQ=255)                                                                                                                        ccAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcg                      >  2:110130/1‑143 (MQ=255)                                                                                                                          aaaaCGCCGGAGAATTTTCACTCCGGCGTTTTCATCTTAAAACAAACCAGAGATTGCCTGATGCGTTACGCTTATCAGGCCTTCCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgcc                    <  2:383452/143‑1 (MQ=255)                                                                                                                            aaCGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgccgc                  >  1:358544/1‑143 (MQ=255)                                                                                                                                 cGGAGAATTTTCACTCCGGCGTTGTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCg             <  1:97904/143‑1 (MQ=255)                                                                                                                                            cACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAc  <  1:1093266/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                              TAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAACGTTCCGGCTGTACCGGCAATTCCGCAGCAGTAATTCACGGCCAAAACGCCGGAGAATTTTCACTCCGGCGTTTTCATCTGAAAACAAACCAGAGATTGCCTGATGCGCTACGCTTATCAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAC  >  NC_000913/1691249‑1691530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.