Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,312,117 A→C 17.8% G211G (GGT→GGG ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,312,1170AC17.8% 122.3 / 4.4 51G211G (GGT→GGGompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (34/6);  new base C (2/8);  total (36/15)
Fisher's exact test for biased strand distribution p-value = 1.91e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCAC  >  NC_000913/2311887‑2312355
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              
aaCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTa                                                                                                                                                                                                                                        >  1:296496/1‑239 (MQ=255)
       aCTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATa                                                                                                                                                                                                                                  <  1:713842/238‑1 (MQ=255)
       aCTGAGCAACAGCTGCGAAGTTTTGGGGTTTGTACGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATa                                                                                                                                                                                                                                  <  2:13954/238‑1 (MQ=255)
           aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGa                                                                                                                                                                                                                             >  1:110990/1‑239 (MQ=255)
           aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGCTCGCCGTTAACGAAGTAAGCAGCGCAGGTCTGAGCATCAGTACGTTTGTAGCTGGAGATCGCACCAACGATACCGAAACCTTATTAATCATAAGTGa                                                                                                                                                                                                                             >  2:756692/1‑239 (MQ=255)
             cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATa                                                                                                                                                                                                                           >  1:714831/1‑239 (MQ=255)
             cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATa                                                                                                                                                                                                                           >  1:267185/1‑239 (MQ=255)
                   cTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAAcc                                                                                                                                                                                                                      >  1:149714/1‑238 (MQ=255)
                                 gcgttGTCCGCCAAACCCAGGGAACGTACGCGAGTTGGGTTGTAGGTCGGGGTGTACTGAGCACCCAGGTAGATTTTGTTAGCGTCGTATTTCAGACCCCCAGTGTGGTTTTCAGCCCGGTCGCCGTTACCGATGTAAGCAGCGGTTTTCTGAGCATCAGTACGTTGGGAGCTGGAGATCGCCCCCCCGATACCGAACCCTTCGTAATCATAAGGGATAGAACCGCCGAcgccgtcgc                                                                                                                                                                                                        <  2:714831/235‑1 (MQ=255)
                                       ttCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCAACGATACCGAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTg                                                                                                                                                                                                 >  2:636593/1‑239 (MQ=255)
                                                          cTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCg                                                                                                                                                                              >  1:291005/1‑239 (MQ=255)
                                                            gcgcggtttGCGTGGTGGGTGTGGGTTTACGAGGACGCCAGTTATATGTTTGTAGGTGCGTATTTGGGCCCACCAGTGTGGGTTTCCTCGCGGTCGCCTTTCCCGGTTTAAGCAGCGGTTTTCTGAGCATCTGTACTTTTGGAGCGGGAATCCCCCCCACCGATCCCAAACCTTTCGTAATCATAAGTAATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCgtt                                                                                                                                                                            <  2:1092051/232‑1 (MQ=255)
                                                                   ttGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAACCCTTCGTCATCATAAGAGATAGAACCGCCGACGCCGTCGCAGTTTGGACGAAGTGCGTCACGACCGATgttagtt                                                                                                                                                                     >  2:74571/1‑239 (MQ=255)
                                                                   ttGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTgttagtt                                                                                                                                                                     >  1:217293/1‑239 (MQ=255)
                                                                          tgaGGTCTGGGGTGTACGGACAAGCCAGGTCGATGTTGTGAGTGTCGTATTGCAGACCACCATGGTATTTATCAGCACGGTCGCCGTTACGGTTGAAAGCGGCGGTGTCCTGGCCAGAAGTACGTTGGGGGCTGAAGATCCCCCCACCGTTACCAAAACCTACGTAATCATAAGTGATACATCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGccc                                                                                                                                                               <  2:955696/237‑2 (MQ=255)
                                                                                cTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCAGCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAACAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTaa                                                                                                                                                        >  1:127182/1‑239 (MQ=255)
                                                                                cTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGGCAGTTGTTAGTTACGCCACTAGTaa                                                                                                                                                        >  1:1062306/1‑239 (MQ=255)
                                                                                    ggTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCGGGGTCGCCGTTACCGATGTAAGCAGCGGGGTTCTGAGCATCAGTACGTTTTGAGATGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATACGTGAGAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGAGCGTCACAACCGTTGTTAGTGACGCCACTAGTaaac                                                                                                                                                      >  2:783708/1‑237 (MQ=255)
                                                                                                        gagttgttgtTGGCTTCGTCTTTCAGCCCACCAGTGTGTGTTTCAGCACGTTCGCCGTTCCCGATGTACGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCCCCGATACCGAAACCTTCATAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGc                                                                                                                                   <  2:267185/234‑1 (MQ=255)
                                                                                                                gttAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTa                                                                                                                           >  1:107202/1‑238 (MQ=255)
                                                                                                                 ttAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACTGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCAGTTTTTAc                                                                                                                          >  1:786468/1‑238 (MQ=255)
                                                                                                                  gagggaggtATTTCAGACCCGTGGTGTAGTTTGCAGGAGGGGCGCCGTTACGTAGATAAGCAGAGGTTTTCTGCGCATCAGCACGTTTGGAGTGGGAGACCGCACCACCGATCCCGAACCTTGCTTAATCAAAAGGTAAAGATCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                                                        <  2:358074/232‑1 (MQ=255)
                                                                                                                   gggcttgtATTCAAGCCCACCAGTGTGGGTTGCAGCAGGGTCGCGGTACGCGATGTAAGCGGCGGTGTTCTGAGCATCAGTACTTTGGGAGCGGGAGACCGCCCCACCGATACCGAAACCTTCGAAATCAAAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                                                        <  2:217293/232‑1 (MQ=255)
                                                                                                                            ttgcacaccaccAGTGTGGTTTTCCGCAGGGCGGCGGTTACGGATGTAAGCGGGGGTGTTCTGAGCATCATTAGTTTTGGAGCTGGATACCGCACCCCCGATACCGAATCTTCCGTAATCATATGTGATAGATCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGaa                                                                                                              <  2:459209/233‑1 (MQ=255)
                                                                                                                                  accaccAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCaa                                                                                                        >  1:502429/1‑239 (MQ=255)
                                                                                                                                    caccaGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAg                                                                                                      >  1:1063597/1‑239 (MQ=255)
                                                                                                                                      ccaGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGt                                                                                                     >  1:1118379/1‑238 (MQ=255)
                                                                                                                                          gttAGGTTTCAGCCCGGTCGCCGTTCCCGATGTAAGCAGGGGTGTTCTGAGCATAAGTGCGTTTGGAGCGGGAGACCGCACCCCCGATACCAAACCCTTCGTAATCAAAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGc                                                                                                                                                                 <  2:9581/172‑1 (MQ=255)
                                                                                                                                                 ttCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGCTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCa                                                                                          >  1:287030/1‑238 (MQ=255)
                                                                                                                                                    aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTaaa                                                                                                                                                       >  1:9581/1‑174 (MQ=255)
                                                                                                                                                    aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTTACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                      >  1:402896/1‑239 (MQ=255)
                                                                                                                                                    aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAcc                                                                                       >  2:104421/1‑238 (MQ=255)
                                                                                                                                                    aGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                      >  1:896168/1‑239 (MQ=255)
                                                                                                                                                          gTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCga                                                                                >  1:625484/1‑239 (MQ=255)
                                                                                                                                                                gTTCCCGATTTAAGCACGGGTGTTTTGGGAAACAGTATTTTGGGAGCGGAAAATCGCCCCCCCGATACGAAACCCTTCGTAACCAAAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                                          <  1:115111/239‑1 (MQ=255)
                                                                                                                                                                              cagcGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGCAGAAGTCAGTGTTACGGTAg                                                            >  1:364496/1‑239 (MQ=255)
                                                                                                                                                                                 gcgttttCTGAGCATCAGTACTTTGGGAGCTGGAGCCCGCCCCCCCGTTACCAAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                                               <  2:945673/212‑1 (MQ=255)
                                                                                                                                                                                     tttCTGAGCATCAGTACTTTTGGAGCGGGAGATCCCCCCACCGATACCAAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGa                                                     <  1:238843/238‑1 (MQ=255)
                                                                                                                                                                                      ttCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCACACAGACCGAAGAAGTCAGTGTTACGGTAGGCAGCGaa                                                    >  2:820103/1‑239 (MQ=255)
                                                                                                                                                                                           ggCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGGTACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTAACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAACAAAGTTCCGGGCGTCAACCAACACGAAGAAGTCCGTGTTACGGTAGGTCCGGACGCCGt                                               >  2:135965/2‑239 (MQ=255)
                                                                                                                                                                                           aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtg                                                                     >  1:945673/1‑217 (MQ=255)
                                                                                                                                                                                           aGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGGGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                               >  1:904528/1‑239 (MQ=255)
                                                                                                                                                                                              aTCAGTACGTTTGGAGCTGGAAATCGCACCACCGATACCGAAACCTTCGTAATCATAAGAGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGCAAAGCCTTCACCAGATGGGGTGACGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAATCAGACCGAAGAAGCCAGTGTTACGGTCGGGCGCGAAGCCGGTa                                             >  2:1136023/1‑238 (MQ=255)
                                                                                                                                                                                                cAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGCCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAca                                           >  1:692298/1‑237 (MQ=255)
                                                                                                                                                                                                     tgTTTGGGGCTGGAGACCCCCCCACCGATCCCAAACCCTTCTTAATAAAAAGGTATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctg                                     <  1:1136023/238‑1 (MQ=255)
                                                                                                                                                                                                       tttGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGAAGGTCGCGAAGCCGTTACCACgcg                                      >  2:811520/1‑235 (MQ=255)
                                                                                                                                                                                                             gCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa                             >  2:69971/1‑239 (MQ=255)
                                                                                                                                                                                                                ggAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGt                          >  2:74960/1‑239 (MQ=255)
                                                                                                                                                                                                                           caccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCg               >  2:1104614/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ccGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                                                        >  2:676165/1‑126 (MQ=255)
                                                                                                                                                                                                                                   ccGAAACCTTCGTAATAATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTAccc                                                                                                                        <  1:676165/126‑1 (MQ=255)
                                                                                                                                                                                                                                        aCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGCGTcacca   >  1:250168/1‑238 (MQ=255)
                                                                                                                                                                                                                                        aCCTTCGTAATAATAGGTGATAGACCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac  <  1:636593/239‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              
AACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGTAGATGTTGTTAGCGTCGTATTTCAGACCACCAGTGTAGGTTTCAGCACGGTCGCCGTTACCGATGTAAGCAGCGGTGTTCTGAGCATCAGTACGTTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCAC  >  NC_000913/2311887‑2312355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: