Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,358,812 A→G 20.1% S126P (TCC→CCC)  puuP ← putrescine importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8120AG20.1% 157.3 / 4.1 74S126P (TCC→CCC) puuPputrescine importer
Reads supporting (aligned to +/- strand):  ref base A (30/26);  new base G (15/2);  total (46/28)
Fisher's exact test for biased strand distribution p-value = 1.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTTCTCGCTGATAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAC  >  NC_000913/1358583‑1359049
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             
gcgTTCTCGCTGATAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTTCCACCCCCACCCCCGGCGGCACTTCCGGGGAGACGGGGGGGagaaagat                                                                                                                                                                                                                                      >  1:919521/1‑239 (MQ=255)
gcgTTCTCGCTGATAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGGCCTTCCGGGGAGGGGGCGGGGagatagga                                                                                                                                                                                                                                      >  2:1109098/1‑237 (MQ=255)
 cgTTCTCGCTGATAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACCGCGGCACTTCCGGGGAGAGGGAGGGGAGATAGGtt                                                                                                                                                                                                                                     >  2:189881/1‑239 (MQ=255)
 cgTTCTCGCTGATAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACCGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGAtt                                                                                                                                                                                                                                     >  1:796184/1‑239 (MQ=255)
             tAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCcaacaa                                                                                                                                                                                                                          <  1:760023/238‑1 (MQ=255)
                      gAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGGTAAAATTGGGAAGAAGGGTAACACCCACACCCCCCGCCGCACTTCCGGGGAGAGGGGGGAGAGAAAGAATTTCGCCAACAACACGGTaaac                                                                                                                                                                                                                >  2:435186/1‑237 (MQ=255)
                            aCCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCCCCCACACCCACGGCGGCACTTCCCGGAAGAGGGCGGAGAGATAGATTTTCGCCCACACGAAGTTAATCAACGGc                                                                                                                                                                                                          >  2:1140529/1‑239 (MQ=255)
                                 aCGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCaaaaa                                                                                                                                                                                                     >  2:347464/1‑239 (MQ=255)
                                           cGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATcc                                                                                                                                                                                           <  1:1032103/239‑1 (MQ=255)
                                              cTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCcag                                                                                                                                                                                         <  1:347464/238‑1 (MQ=255)
                                                        aTGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGAc                                                                                                                                                                               >  2:588101/1‑238 (MQ=255)
                                                              tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCCAGGCGGCACTTCCGGGGAGAGGGCGGAGAGGTAGATTTTTGCCAACAAAACGTTAATCACGGGCAAAAAGAGATAATGCAGCAGCGATGACCAGCCg                                                                                                                                                                         >  1:602754/1‑238 (MQ=255)
                                                              tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCg                                                                                                                                                                         >  1:112302/1‑238 (MQ=255)
                                                              tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCg                                                                                                                                                                         >  1:1217494/1‑238 (MQ=255)
                                                              tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACCAAGGGTACACCCACCCCCACGGCGGCACTTCCGGGGAGAGGGCGGAGGGATAGAATTTTCCCCACAAGACGTTAATCATCCGCCAAAAGGGATAATCACACAGCGATGACCAGCCgc                                                                                                                                                                        >  2:39405/1‑237 (MQ=255)
                                                              tCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGAAGCAGGGTACAACCCACACCCACCGCGGCCCCTCCGGGAAGAGGGCGGAGAGACAGATTTTCCCCCACAAGACGGTAATCATCCGGAAAAAGAGAGAATCCACCAGCGGTGCCCAGCCg                                                                                                                                                                         >  2:1187789/1‑238 (MQ=255)
                                                               cccTGAACACCCAGGGAGATAAACACCACCAGTATGGAGATTTGCACCAGTACAAACAGGGTAGTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCTCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGcc                                                                                                                                                                          <  2:381335/236‑1 (MQ=255)
                                                                 cTGAACTACCAGGAAGATAAACACCACCAGGATGGAGAGTTGCAGCAGTACAAACAGGGTATTGAAGTTTGCGACCAGGTTGACGCTCTTCAGATGCGCGGCGGTTAAAATGGCGACAAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCa                                                                                                                                                                     <  1:88102/239‑1 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCCCTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACCAGACGTTAATCATCGGCCAAAAGAGATAATCCAGCAGCGGTGACCACCCGACCATAAATc                                                                                                                                                               >  2:799738/1‑238 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGGGGTAGGTTTTCGCCCACAAGACGGTAATCATCGGCAAAAAGAGATAATCCAGCCGCGATGAACAAcctgccctaacac                                                                                                                                                               >  2:751066/1‑227 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCCAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCCTAAAta                                                                                                                                                               >  2:540554/1‑237 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATc                                                                                                                                                               >  2:424977/1‑238 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCcagcag                                                                                                                                                                                      <  2:369709/215‑1 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATc                                                                                                                                                               >  1:133592/1‑238 (MQ=255)
                                                                        aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGGTAATCATCGGCAAAAAGAGATAATCcagcag                                                                                                                                                                                      >  1:369709/1‑215 (MQ=255)
                                                                                       aacaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAAt                                                                                                                                               <  1:1187789/237‑1 (MQ=255)
                                                                                        ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCCCCCACCCCCACGGCGGCACTTCCGGGGCGAGGGGGGGGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCACCCGACCATAAATCCCACGTGCGGGTTAAt                                                                                                                                               >  2:54675/1‑238 (MQ=255)
                                                                                           ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCcag                                                                                                                                                                                         <  1:113605/193‑1 (MQ=255)
                                                                                           ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCCGCCGACCATAAATACGACGTGCGGGTTAATCg                                                                                                                                             >  1:1044830/1‑237 (MQ=255)
                                                                                           ccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAAACcag                                                                                                                                                                                         >  2:113605/1‑193 (MQ=255)
                                                                                              tgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAAACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCCACCATAAAACCGACGTGCGGGTTAATCGACttt                                                                                                                                        >  1:971422/1‑239 (MQ=255)
                                                                                               gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtttt                                                                                                                                       <  1:840848/239‑1 (MQ=255)
                                                                                               gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTg                                                                                                                                      <  1:39405/239‑1 (MQ=255)
                                                                                                 tgGAGATTTGCCCCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTg                                                                                                                                      <  1:918792/238‑1 (MQ=255)
                                                                                                      aTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCCCTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCAACAAGGCGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGACGCACATAAATCCGACGTGCGGGGTAATTGACTTTTTcgcgga                                                                                                                                >  2:60273/1‑237 (MQ=255)
                                                                                                      aTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAACCCACCATAAATCCGACCTGCGGGTTAAACGACTTTTGCGCGTa                                                                                                                                >  2:268855/1‑239 (MQ=255)
                                                                                                       tttGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGt                                                                                                                                 <  1:608480/237‑1 (MQ=255)
                                                                                                        ttGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCAACGGGAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGGCTTTTGCGCGGAgg                                                                                                                              >  1:1224965/1‑239 (MQ=255)
                                                                                                         tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATTAATCCGACGTGCGGGTTAATCGACTTTTTCGCGTAGGt                                                                                                                             >  1:65294/1‑239 (MQ=255)
                                                                                                                gTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGGACTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACCAGACGTTAATCATCGGCAAAAAGAGAAAATCCAGCAGCGATGACCAACCGACCATAAATCCGACGTGCGGGTTAATCCGCTTTTTGCCGTAGGTAAAGGc                                                                                                                       >  2:1081321/1‑238 (MQ=255)
                                                                                                                          ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGTTACCCCCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAACCGACCATAAATCCCACGTGCGGGGTAATCGACTTTTTCGCGTAGGGAAAAGCCCAACCCGcc                                                                                                             >  2:582089/1‑238 (MQ=255)
                                                                                                                          ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGcc                                                                                                             >  1:16427/1‑238 (MQ=255)
                                                                                                                          ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGAcc                                                                                                             >  1:663483/1‑238 (MQ=255)
                                                                                                                           gTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGCGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGGGGGGGGGTAGATTTTTGCCAACCAGAAGTTAATCATCGGGAAAAAGAGATAAACCAGCAGCGGAGAACAACCGACCATAAATCCGAAGTGCGGGGTAATAGACTTTTGGGCGGGGGGGTAGGCCCAACCGGccc                                                                                                            >  2:80833/1‑237 (MQ=255)
                                                                                                                           gTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTTATCATCGGCAAAAAGAGATAATCCCGCAGCGGTGACCAGCCGACCATAAATCCGACGTGCGGGTTAAATTACTTTTTCGCGTAGGTATAAGCCGAACCCGccg                                                                                                            >  2:1146722/1‑237 (MQ=255)
                                                                                                                                  aGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAAc                                                                                                    <  1:435186/239‑1 (MQ=255)
                                                                                                                                      aGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAAATGGc                                                                                                >  1:63744/1‑239 (MQ=255)
                                                                                                                                               gTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGtt                                                                                       >  1:1219334/1‑239 (MQ=255)
                                                                                                                                                ttGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACTCCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGt                                                                                        <  1:371544/237‑1 (MQ=255)
                                                                                                                                                    cGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGATAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCg                                                                                  <  1:1109098/239‑1 (MQ=255)
                                                                                                                                                        ctTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTTCGCGTAGGGATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTa                                                                                >  1:180140/1‑237 (MQ=255)
                                                                                                                                                         ttCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGGACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGAAGACCAGCCGACCATAAATCCGAAGGGCGGGGTAATCGCCTTTTTCGCGTAGGTGTAGGCCGCACCGGCCTCCGGGAACTGGCGAACCAGTTTGCCgga                                                                                >  2:185481/1‑234 (MQ=255)
                                                                                                                                                         ttCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCAACCGCAAAAAGAGATAATCCAGCAGCGATGACCAGACGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAAGCCGAACCGGCCTCCGGAAAATGGCGAACCAGTTTGCCGtc                                                                                >  2:636843/1‑235 (MQ=255)
                                                                                                                                                           caggctCGCGGGGGTTAAAGTGGCGACGAAGGTTACCAGCCTCACCCTGGGCGGCACCTACGAGACGAGGGCGGAGAGATCGATTTTCGCAACCAAGACGTTTATCTTCGGCAAAAAGGGATAATCCAGCAGCGATGTCCAGCCGACCATAAACCCGATGTGCAGGTTAATCGACATTAGCGCGTAGGTATAGGCCGAGCCGGCCCCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGa                                                                           <  2:718291/234‑1 (MQ=255)
                                                                                                                                                           cAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGa                                                                           <  1:423640/239‑1 (MQ=255)
                                                                                                                                                                tCGCGGGGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCg                                                                      <  2:602754/239‑1 (MQ=255)
                                                                                                                                                                tCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCg                                                                      <  1:60273/239‑1 (MQ=255)
                                                                                                                                                                   cggcggTTAAAATGGCGACGAAGGGTACCACCCCCACCCACGGCGGCCCTTCCGGGGAGGGGGGGGGGAGGTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGGATAGGCCCAACCGGCCTCCGGAAACCGGCGAACCCGTTTGCCGTTGCCGATAGCggga                                                                   >  2:581396/1‑237 (MQ=255)
                                                                                                                                                                    ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGCTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTa                                                                   <  2:493277/238‑1 (MQ=255)
                                                                                                                                                                       ggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACa                                                               <  1:799738/239‑1 (MQ=255)
                                                                                                                                                                       ggTAAAAACTGCGACGAAGGTTACCCCCCACCCCCGCGGCGGCACTTCCGGGAAGACGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTGGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACa                                                               <  2:112446/239‑1 (MQ=255)
                                                                                                                                                                                        aGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACTGGCGAACCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcgc                                               <  1:636843/238‑1 (MQ=255)
                                                                                                                                                                                                    acacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCg                                                                      >  1:563652/1‑203 (MQ=255)
                                                                                                                                                                                                    acacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCg                                                                      <  2:563652/203‑1 (MQ=255)
                                                                                                                                                                                                    acacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGAc                                  >  1:680375/1‑239 (MQ=255)
                                                                                                                                                                                                           cggcggCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGa                            >  1:83936/1‑238 (MQ=255)
                                                                                                                                                                                                            ggcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAAc                          >  2:649530/1‑239 (MQ=255)
                                                                                                                                                                                                                gctcTTCCGGGAAGAGGGCGGCGAGATAGATTTTCGCCAACAAGACGTTCATCATCGGCTAAAAGAGATAATCCAGCAGCGATGACAAGCCGACCCTAACTCCGACGTGCGGGTTAATCGACTTTTGCGAGTAGGTATAGGCCGAACCATCGTCCGGTAACTGGCGAACCAGGTTGCCGTAGCTGATAGCGGTAAACCGCACACCCCCCAGACACAGCAACTACAACGCCGGAAAGTg                       >  2:959077/4‑238 (MQ=255)
                                                                                                                                                                                                                  ccTTCCGGGAAGAGTGCGAGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGAAAAAAGAGATAACCCAGCAGCGATGACCACCCGACCATAATTCCGACGGGCGGGTTAACCGGCTTTTGCGCGTAGTTATAGGCCGACCCGTCCGCCGGAAACTGGCGAACCAGTTTGCCGTAGCGGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAcc                    <  2:919521/238‑1 (MQ=255)
                                                                                                                                                                                                                  aCTTCCGGGAAAGGGGCGAGGAGAAAAATTTCCGCAAAAAAGACGTTAACCATCGGAAAAAAGAGATAATCAAGCGGCGATGACCAGCGGACAATAAATCCGACGTGCGGGTTAACCGCCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACGGGAGAACAAGTTTGCCGTAGCTGATAGGGGAAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAcc                    <  2:1224965/239‑1 (MQ=255)
                                                                                                                                                                                                                          gAAGAGGGGGGAAGAAAAAATTTCCGCAAAAAAGACGTTAATAATCGGAAAAAAGAGAAAACCCAGAACGGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTa             <  2:1219334/238‑1 (MQ=255)
                                                                                                                                                                                                                               gggCGGAAAGATCGATTTTCGCCAACAAGACGTTAACCATCGGCAAAAAGAGAAAATCCAGCAGCGATGACCAGCCGACCATAAATCCGCCGTGCGGGTTAACCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAACCGGGCGAACCAGTTTGCGGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGcc        <  2:83936/238‑1 (MQ=255)
                                                                                                                                                                                                                                ggCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCgg      >  1:907254/1‑239 (MQ=255)
                                                                                                                                                                                                                                ggCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCgg      >  1:807457/1‑239 (MQ=255)
                                                                                                                                                                                                                                     agagaTAGATTTTCGCCAAAAAGACGTTAATCATCGGAAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAc  <  1:424977/238‑1 (MQ=255)
                                                                                                                                                                                                                                     agagaTAGATTTTCGACAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGAAAACTGGCGAACCAGTTGGCCGTAGCTGATGGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAc  <  1:54675/238‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             
GCGTTCTCGCTGATAAACGGCTGAAGTGACCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAC  >  NC_000913/1358583‑1359049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: