BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,506	C→A	12.3%	T15K (ACA→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,506	0	C	A	12.3%	262.3 / 2.6	116	T15K (ACA→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (42/56); new base A (5/11); total (49/67)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

AGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206281‑3206742

aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGAAAAAAGTGACAGAGATGTTCGc                                                                                                                                                                                                                                      >  2:894581/1‑238 (MQ=255)

aGCGAAGGGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCGGGCGCAATATTCATTCTTATTTCCGCGTTGATTCACGAACTGTTAATTACTTATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                                     <  2:800534/239‑1 (MQ=255)

  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAAAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCACGTAACTAA‑TTGGCAGAGAtttagcgat                                                                                                                                                                                                                                    >  2:147067/1‑231 (MQ=255)

  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTACAAATTC‑AAAAAATTGAGTTTCACT‑GATGAGCGCAAATAAAAAACAC‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAAATTACATAGATTGTTTCTaa                                                                                                                                                                                                                                   >  1:800534/1‑239 (MQ=255)

       tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                                                                                                                                                                                              <  1:1082447/239‑1 (MQ=255)

              cGCAAAACATTCCCA‑TGCCTTGCTTCGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCAAACTTTTAATTACATATA‑CTCGGTGGCAAAAAATAAAAACCAAAATTTTTGGTGGTAGTTTGGGGAGGTGAATTTTTCCAGAAAAAAAC‑AAAAACTGAGGTTGCAAT‑GATGAGCGAAAGTAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAt                                                                                                                                                                                                                       <  1:618457/239‑1 (MQ=255)

                 aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTAACAGAGTTTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                                                   <  2:1306196/233‑1 (MQ=255)

                 aaaaCATTGCCA‑TTCCCTGTTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAATTGTTAATTACATATT‑CACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                                                   <  1:1220250/239‑1 (MQ=255)

                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAAAGAATGTCGCTAACTTTAACGCCATG‑At                                                                                                                                                                                                                   >  1:251319/1‑239 (MQ=255)

                   aaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑Attt                                                                                                                                                                                                                 <  1:1192224/239‑1 (MQ=255)

                        tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAAAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGGAAAAAATTAAAGAGATTGGTGCTCACTTTTACGCCATG‑Aatttttc                                                                                                                                                                                                             >  1:642912/1‑234 (MQ=255)

                          ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAA‑‑‑‑CAA‑‑‑GCAAAAAGCA‑GCGGATAAGTTGACAGAGATTTTTGCTAAATTTACCCACATG‑Attt                                                                                                                                                                                                                 >  1:558935/1‑239 (MQ=255)

                            ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GCGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                                                                         <  1:677346/237‑1 (MQ=255)

                            a‑TTCCCTGCTCTGGCACAATATTCTTTCTTTTTTTCGCGTTGATTCACGAACGGTTAATTACATTTT‑CACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                                                        <  1:482289/239‑1 (MQ=255)

                                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ACGAATAAGATTAGAGAGAATGTGGGTCACTTTACCGCCCTG‑ATTTCTATCCGAATGc                                                                                                                                                                                                     >  2:970454/1‑239 (MQ=255)

                                         ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATCGAGTTTTCCT‑GATGTGGGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAATTTACAGAGAATGTCGCTAAATTTAACTCCATG‑TTTTTTACCCGCATGACcgatgtgg                                                                                                                                                                                            >  1:623602/1‑236 (MQ=255)

                                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GCA‑CAGAATAAGTTTTCAGAGATTGTCGTTACCTTTATAGCCCCG‑CTTTCTATCCGAATGccccgggacgt                                                                                                                                                                                           >  2:968220/1‑230 (MQ=255)

                                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCAT‑GATGAGCGAAAGAAAAAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTGACAGAGATTGGCGCTAACTTTTCCGCCATG‑ATTTTTACCCCAATGCCtgattaaggg                                                                                                                                                                                          >  1:1349641/1‑233 (MQ=255)

                                            gcAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATT‑CAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAAACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                                          <  1:970454/238‑1 (MQ=255)

                                                tatTCTATCTTATTTTCGAGTTTATTCACGAACTGTTAATTAAATATC‑CAGGGTGCCAAAAAATATAAAACCTCATTTGTAGGGGAAGTTTGTGGGGGTGAATTTTCCAAGACAAATAC‑AAAACCTGGAGTTGCCAT‑GAGTAGCAAAAATAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTGACAGAATTTGCCGCTATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  2:1286999/239‑1 (MQ=255)

                                                aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAATTGTTCAAAAAAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAG‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑ACA‑TGAAATAAGTGGAAGGGAATTGGCGCAAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  2:1291506/234‑1 (MQ=255)

                                                        tttATTTTAGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGAAAAAAAAATAAAACCACTTTTTAAGTGTGAGTTTGTGGCGTGGAATTTTTCAAGAAAAAAAC‑AAAAACTGGAGTTGCCAT‑GTGAAGAAAAATTAATAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCCCTAACTTTTCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                             <  1:968220/238‑1 (MQ=255)

                                                        cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                                                                                                             <  1:328413/239‑1 (MQ=255)

                                                         ttATTTTCGCGTTGATTCACGAACTGTTAATTACATTGT‑CACGGTGGAAAAAAATAGAAACCCACATTTGTAGTGGTATTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGACTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                            <  1:359612/239‑1 (MQ=255)

                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑ACA‑GAGAAACAGATTAAAAAAATTGTAGGTAAATTTTAAGCCATG‑AGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACCaaac                                                                                                                                                                           >  2:958061/1‑238 (MQ=255)

                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATTGAGTTTGCCT‑GATGAGAGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑ACA‑GAGAAAAAATTGACAGAGATTGTTGCTAAATTTAACGCCAAG‑ATTTTTACCCCAAAGCCCGATTACGGGGGGGATAAACTaaaa                                                                                                                                                                           >  1:459018/1‑239 (MQ=255)

                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTAAGACAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑AAA‑ATGAAAAAGATGGCAGAGAGTGTTGCTAACTATACCGCCCTG‑ATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACAaaaa                                                                                                                                                                           >  2:475605/1‑239 (MQ=255)

                                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTACGAAAAAAAC‑AAAAAATGGAGTTTTCAT‑TGTGGGAGGAAAGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAAAAAATTGACAAAGATTGTGGCGTACTTTTTCGCCCTG‑ATTTCTTTCCGAATACCTGATGACGTGGGGGGTATACAaaaaaa                                                                                                                                                                         >  2:582594/1‑237 (MQ=255)

                                                                 gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCAGACAAAAAA‑AAAAACAGTAGGGTCCTG‑TATTGGGGGAAGTTATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAAATTGCAGAAATTGTCGCTAACATTACCCCCCAG‑AATTCTTCCCAAATGCCTGATTACGCGGTGGATAAATTaaaaagagtta                                                                                                                                                                    >  2:1151044/1‑232 (MQ=255)

                                                                     gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAATTTTTACAGACAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCG‑GTGAATAAGTTGACAGAGTTTTTCACTAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg‑g                                                                                                                                                                <  2:1280570/231‑1 (MQ=255)

                                                                                  gTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCCT‑GATGAGGGAAAGTAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTTACAGAGAATTTTGCTAACTTTAACCCCATG‑ATTTTTACCCGAAAGCCTTAATAAGTGGGGGGTAAACTAAAAAAGATAAAA‑GAAGGCGAAAcagcg                                                                                                                                                  >  1:652970/1‑235 (MQ=255)

                                                                                   ttAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAgaga                                                                                                                                                                                                                                             <  1:697647/149‑1 (MQ=255)

                                                                                   taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  1:263711/202‑1 (MQ=255)

                                                                                     aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTACCAGACAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGtc‑gtcg                                                                                                                                              <  2:595367/239‑1 (MQ=255)

                                                                                       ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGAt                                                                                                                                            <  1:1068744/239‑1 (MQ=255)

                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAAAAAATTGAAAGAGAGTGTCGCTAACTTTACCGCCATG‑ATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACTAAAAAACCTAAAG‑GATACCGAAAAGTC‑CTCGGTgggg                                                                                                                                        >  1:631210/1‑239 (MQ=255)

                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATTGAGTTTTCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAGTTGAAAGAGATTGTCGCTAACTTTAACGCCCTG‑ATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAAATAAAAAAGCTAAAG‑GATGACGCAACGCA‑GGCGATgggg                                                                                                                                        >  1:1388078/1‑239 (MQ=255)

                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTATAACAAATAA‑AAAAACAGGAGTTGGCAT‑TATGAGGGGAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑GGGGAAAAGTTTAGAGGGAATGTTGCTACATTTATACCCATGAATTATTACCCAAAAGCC‑GGTGTTGTGGGGGATAAAACAAAACAGCTAAAA‑AACTTCGGAAAGTC‑GTCGACgggg                                                                                                                                        >  2:1019021/1‑239 (MQ=255)

                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAATAC‑AAAAAAGGGGGGTGCCGT‑GGTGGGCTGGAGTAATAAAAAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GAA‑ATAACTAAGTTTTCTGACATTTGGGCTAAATTTAAATCAATG‑CGTTCTTCCCCAACCCCTGAGTGCGCCGGGGGTAAAAAAAAACAGCTAAAGAGTTTCCTGAAC‑TC‑GTCGATgggg                                                                                                                                        >  2:229326/1‑239 (MQ=255)

                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGa                                                                                                                                                         <  1:1049051/223‑1 (MQ=255)

                                                                                           atatT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa                                                                                                                                                                     <  1:829964/212‑1 (MQ=255)

                                                                                            tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAATTTTTACATACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTGGACAGAGATTGTCGTTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                                                       <  1:1403772/239‑1 (MQ=255)

                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                                                       <  1:469404/239‑1 (MQ=255)

                                                                                            attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                                                       <  1:1064968/237‑1 (MQ=255)

                                                                                             tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTAACAGATATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                                                       <  2:251319/233‑1 (MQ=255)

                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGAAAAAAAA‑AAAAAATTGAGTTGGCCT‑GGTGGGTGGAAAAAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑ACA‑AGGGATAAATTGGAAGAGATTGTTGCTaa                                                                                                                                                                                                                                   >  2:697647/1‑149 (MQ=255)

                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGGGGTGCCAT‑GATGGGCGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGCCCTG‑ATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACTAAAAACGCTAAAA‑GAAGCCGAAAAGTC‑GGTGATggggga                                                                                                                                      >  1:1374632/1‑237 (MQ=255)

                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAAATGAGTTTGCAT‑GGTGAGCGGAAAAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑AGGAATAAATTTACAAAGAATGTCGCCAACTTTACCGCCCTT‑ATTTTTAACCCAATGGCTGATGACGTGGGGGGTAAAATAAAACAACAAAAA‑GATTCCGCAACATC‑GTCGATggggga                                                                                                                                      >  1:1232761/1‑237 (MQ=255)

                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGaa                                                                                                                                      <  1:1151052/239‑1 (MQ=255)

                                                                                                  aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAA‑AAAAAATTGAGTTGCCCT‑GATTGGGGGAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAATTGACAGAGATTTTCGCTAACTTTTCCGCCATG‑ATTTTTACCCGAATGCCTGATTACGTGGGGGGTAAAATAAAAAAGATAAAG‑GATGCCGAAAAGTC‑GGCGATGGGGGAAAtt                                                                                                                                  >  1:1201523/1‑239 (MQ=255)

                                                                                                    gagaaaaaaaaaTA‑AAAACCAAATTTTTGTGGGTGGTTTGTGAGGGTGAATTTTTCAAAAAAAATAA‑AAAAACGGGAGTTGCCTG‑GATGAGAAAAATAAAAAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAGGTTGACAGAGATTGCCGATAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                                                                <  2:459018/232‑1 (MQ=255)

                                                                                                     ggggCAAAAAAAAAAAAACCAAATGTTTAGTGGTAGTTTGGGGCGGTGAATATTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAATAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTGACAGAGATTGTCGCAACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAAGTAt                                                                                                                                <  2:576654/236‑1 (MQ=255)

                                                                                                     gTGGAAAAAAAAAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAA‑AAAACCTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                                                                <  2:623602/238‑1 (MQ=255)

                                                                                                      tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACATCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATc                                                                                                                               <  1:134290/238‑1 (MQ=255)

                                                                                                           aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAGATTTTCCAAACAAAAAA‑AAAAAATGGAGTTGACCT‑GGTGGGCGGAAGAAAAAAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑ACA‑AAGCAAAAGATGGCAGAGATGTTCGATCAATTTACTGCCCTG‑ATTTCTAACCGAAAGCCCGACTACGGGGTGGAGAAACAAAAACAGCCAAAG‑GAGGCCCACA‑GTCAGTCGGTGGGGAAAATTATCTACCa                                                                                                                          >  2:1323481/1‑238 (MQ=255)

                                                                                                              aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTTCCAAAAAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTGACAGAGATTGTCGCAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                                     <  2:1201523/239‑1 (MQ=255)

                                                                                                              aaTAAAAACCCACATTTTGAGTGGGAGTTGGGGGGGGTGAATTTTTCCAAAAAAAAAC‑AAAAAGGGGAGTGGCAAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCG‑GTAAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                                     <  1:229326/239‑1 (MQ=255)

                                                                                                              aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAATTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑TGGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                                     <  1:40417/239‑1 (MQ=255)

                                                                                                              aaTAAAAAACCACATTTGTAGTGGTAGTGTGTGGCGGTAATTTTTTCAAGAAAAATAC‑AAAAACGGGAGTTGCCAG‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAGCAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                                     <  2:1374632/239‑1 (MQ=255)

                                                                                                                tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                                     <  2:77967/129‑1 (MQ=255)

                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGATGACAGAGATTGTCGCCAACTTTACCGCCCTG‑ATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACTAAAACAGCTAAAA‑GATGCCCAAACGAC‑GTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                                                                         >  1:957057/1‑238 (MQ=255)

                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAAATGACAAAGATTGTCGCCAACTTTTCCg                                                                                                                                                                                                                           >  1:77967/1‑129 (MQ=255)

                                                                                                                                       ggTTTGGGGTGGAAAATTTTACAAAAAAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                                            <  2:652970/238‑1 (MQ=255)

                                                                                                                                             tgGCGGTGAATTTTTCCAGACAAAAAC‑AAAAATTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGAc                                                                                       <  1:669602/238‑1 (MQ=255)

                                                                                                                                               gCGGTAAATTTTCCAAGACAAATAA‑AAAAACTGGAGTTGCCAA‑GATGAGCAAAAGTAATAAGCAAAGGGC‑AAGGG‑‑‑‑‑‑GCA‑GTGAAAAAGTTGACAGAGATGGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                                            <  2:558935/238‑1 (MQ=255)

                                                                                                                                                   taaaTTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGAACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                                                                 <  1:147067/236‑1 (MQ=255)

                                                                                                                                                         ttaccAAACAAAAAA‑AAAAACTGAGTTTCCCAG‑GAAGAGCAAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑CGA‑GGAAATAAGTTGAAAGAGTGTGTCGTAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          <  2:1232761/236‑1 (MQ=255)

                                                                                                                                                                      taaaaaaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                                              <  1:642776/236‑1 (MQ=255)

                                                                                                                                                                         aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGGACAGATTTTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                                           <  1:795382/239‑1 (MQ=255)

                                                                                                                                                                           aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                         >  2:1250640/1‑239 (MQ=255)

                                                                                                                                                                            aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAAAAAGTTGACAGAGTTTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  2:1349641/239‑1 (MQ=255)

                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:615170/239‑1 (MQ=255)

                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:794496/239‑1 (MQ=255)

                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGAAAGAGTTGGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:905093/239‑1 (MQ=255)

                                                                                                                                                                            aaCTGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:582594/239‑1 (MQ=255)

                                                                                                                                                                            aaCTGAAGTTGCCAT‑GATGAGCGAAATTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAATAAGTTGGCAGAGATCGTACCTAATTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:793625/239‑1 (MQ=255)

                                                                                                                                                                             aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                                       >  2:931448/1‑239 (MQ=255)

                                                                                                                                                                                 gAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑CATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCACAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGCACCCGCGGAGAtt                                                   >  2:570219/1‑239 (MQ=255)

                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                                             >  2:1112121/1‑197 (MQ=255)

                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:545939/1‑239 (MQ=255)

                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:561009/1‑239 (MQ=255)

                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                                             <  1:1112121/197‑1 (MQ=255)

                                                                                                                                                                                                     aaGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg                                >  1:909508/1‑239 (MQ=255)

                                                                                                                                                                                                         aaaaaGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAAAAATTAGAAAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                            <  1:355740/236‑1 (MQ=255)

                                                                                                                                                                                                          ataaGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                            >  2:137945/1‑238 (MQ=255)

                                                                                                                                                                                                                      c‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                    >  2:410470/1‑238 (MQ=255)

                                                                                                                                                                                                                      c‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                    >  1:910312/1‑238 (MQ=255)

                                                                                                                                                                                                                      c‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                    >  2:1054292/1‑238 (MQ=255)

                                                                                                                                                                                                                      c‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTATCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                    >  2:317067/1‑238 (MQ=255)

                                                                                                                                                                                                                                    cA‑GTGAATAAGTTGACAGAGATTGGCGCAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   <  1:319521/235‑1 (MQ=255)

                                                                                                                                                                                                                                        tGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCCCTGCtt            >  1:1181337/1‑239 (MQ=255)

                                                                                                                                                                                                                                        agAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGATCCCGCTTCCCACTGCtt            >  2:174114/2‑239 (MQ=255)

                                                                                                                                                                                                                                            tAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  1:601997/239‑1 (MQ=255)

                                                                                                                                                                                                                                            tAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  1:971447/239‑1 (MQ=255)

                                                                                                                                                                                                                                            tAAGTTGAAAGAGATTGTCGTTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  1:792421/239‑1 (MQ=255)

                                                                                                                                                                                                                                               gTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGa       >  1:519469/1‑237 (MQ=255)

                                                                                                                                                                                                                                               gTTGAAAGAGATTGTCGTTATCTTTACCGCCATG‑ATTTCTACCCGAAGGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  2:1167874/188‑1 (MQ=255)

                                                                                                                                                                                                                                                  gACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:952502/1‑239 (MQ=255)

                                                                                                                                                                                                                                                    cAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATa                                                                                                                        <  2:1036024/120‑1 (MQ=255)

                                                                                                                                                                                                                                                    cAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATa                                                                                                                        >  1:1036024/1‑120 (MQ=255)

AGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206281‑3206742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`