New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 458313751 (1.050)4 (0.090) 4/270 6.7 7.9% intergenic (‑89/+112) hsdM/hsdR DNA methyltransferase M/endonuclease R Type I restriction enzyme
?NC_000913 = 4583224 44 (0.940)intergenic (‑176/+25) hsdM/hsdR DNA methyltransferase M/endonuclease R Type I restriction enzyme
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTG  >  NC_000913/4582995‑4583279
                                                                                                                                              |                                                                                                                                              
gcgcAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAgg                                                                                                                                                <  2:751023/143‑1 (MQ=255)
  gcAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGtt                                                                                                                                              <  1:318964/143‑1 (MQ=255)
      gTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCAt                                                                                                                                          >  2:522185/1‑143 (MQ=255)
          tCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGCTGcctctgc                                                                                                                                      >  2:204789/1‑137 (MQ=255)
            gCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTAAGTTACGTAAGTTAGGGAGCGGGGAAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAGTATACACACAATCATCCCGGTTGCATCAAggc                                                                                                                                    >  1:509217/1‑143 (MQ=255)
             cacggcttccaaggcgtcGCGACGGGAGCGTTATTGTTCATTGTGAGCGCCGTAAATTAAGGAGCGGCCAAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAggcg                                                                                                                                   <  1:44970/143‑1 (MQ=255)
                 gcttccacagcttcGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCAGCGAGCAGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCCTTCAGGTTGCATCAAGGCGGCaa                                                                                                                               <  1:275833/143‑1 (MQ=255)
                         agcttcGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAgtgagtga                                                                                                                       >  1:552336/1‑143 (MQ=255)
                                   ctaGATCGGTATTGTTCATGGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGa                                                                                                             <  2:78014/141‑1 (MQ=255)
                                    cAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCCGGTTGCATCAAGGCGGCAAGGGAGTGAATCCCCGGGAg                                                                                                            >  2:694640/1‑143 (MQ=255)
                                     aGATCGTGATTGTTCATTGTGAGTTCCGTGAATTAAGCAGCGGCGCAAATTCATCGGGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGc                                                                                                           <  1:720269/143‑1 (MQ=255)
                                       aTCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGt                                                                                                         >  1:706026/1‑143 (MQ=255)
                                        tCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGCGCGTa                                                                                                        >  2:495966/1‑143 (MQ=255)
                                         cGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTAc                                                                                                       >  2:140992/1‑143 (MQ=255)
                                           ttATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAg                                                                                                     >  2:205578/1‑143 (MQ=255)
                                             atcgTTCATTGTGAGTTCCGTAAATGAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGaa                                                                                                   <  1:504834/140‑1 (MQ=255)
                                             aTTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGaa                                                                                                   >  1:689811/1‑143 (MQ=255)
                                                 ttCATTGTGAGTTCCGTAAATTACGCGACAAACGAAACTAAGTGTGCCGCAGAAGAAGAAATTGACGAGATTAAAATCCATGATATCATTCAATTTACATCATTGCGGCAAGTGAGTGAATCTCCGGGGGCGTACAGAAGTAc                                                                                               >  2:674702/1‑143 (MQ=255)
                                                 ttCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTAc                                                                                               >  1:803634/1‑143 (MQ=255)
                                                 ttCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTAc                                                                                               >  2:547435/1‑143 (MQ=255)
                                                     ttGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGCATCCCCGGGAGCGTACAGAAGTACGTGa                                                                                           >  1:407027/1‑143 (MQ=255)
                                                         gAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCgg                                                                                       <  1:522185/143‑1 (MQ=255)
                                                          aGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCggg                                                                                      <  2:29446/143‑1 (MQ=255)
                                                                  aaaTTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGa                                                                              <  1:48446/143‑1 (MQ=255)
                                                                      tAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAgtgagtga                                                                                                                       <  1:106493/98‑1 (MQ=255)
                                                                      tAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAgtgagtga                                                                                                                       >  2:106493/1‑98 (MQ=255)
                                                                       aaGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTAAAGAAGTACGTGACCGGGGTGAACGAGCGCa                                                                         >  1:520750/1‑143 (MQ=255)
                                                                          cagcGGCCCAAATTCAACGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGcc                                                                      <  1:498863/143‑1 (MQ=255)
                                                                           agcGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGGGCGTACAGAAGTACGTGGCCGGGGTGGACGAGCGCGGCCa                                                                     >  1:67026/1‑143 (MQ=255)
                                                                                   aaaTTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGCAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCagag                                                             >  2:624318/1‑143 (MQ=255)
                                                                                    aaTTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAgg                                                            <  2:87249/143‑1 (MQ=255)
                                                                                       tCATCCAGCCGCAGAAGAAGAAATTGCCGAGGGTAATAGACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGGGCGTATAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAg                                                         <  2:889646/143‑1 (MQ=255)
                                                                                           cGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCGAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGCGCCAGCCTg                                                     >  2:147842/1‑143 (MQ=255)
                                                                                            gAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGa                                                    >  1:807140/1‑143 (MQ=255)
                                                                                             aGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGaa                                                   <  2:552336/143‑1 (MQ=255)
                                                                                               ccGCAGGAGAAGAAAGTGCGGAGGGTAATATGCGCAAGCTCAGTCAGGTTGCAGCCAGGCGGCAAGTGAGTGAAGCCCCGGTAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAgg                                                 <  2:117930/143‑1 (MQ=25)
                                                                                                cGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGa                                                >  1:130364/1‑143 (MQ=255)
                                                                                                cGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGa                                                >  1:320166/1‑143 (MQ=255)
                                                                                                 gCACAAGAAGAAACTGCAGAGGGTAACATACACAAGATCATGCAGGTTGCATCAAGGCGGCAAGGGCGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGAt                                               <  2:885924/143‑1 (MQ=37)
                                                                                                    gaagaagaaATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGaa                                            <  1:732352/143‑1 (MQ=255)
                                                                                                              ttGCCGAGGGTAATATACACAAAAGCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACgt                                  <  2:832055/143‑1 (MQ=255)
                                                                                                                 ccGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTc                               >  1:743953/1‑143 (MQ=255)
                                                                                                                     gggTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGc                           <  2:611672/143‑1 (MQ=255)
                                                                                                                        tAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTATGTGTCCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAg                        <  1:500240/143‑1 (MQ=255)
                                                                                                                         agtataCACAAAATCATTCAGGTTGCATCAAGGCGGCAAGGGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGc                       <  2:608652/141‑1 (MQ=255)
                                                                                                                               cacaAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTcc                 <  1:140992/143‑1 (MQ=255)
                                                                                                                                acaAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTccg                >  1:294488/1‑142 (MQ=255)
                                                                                                                                  aaaaTCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAg              >  1:662552/1‑143 (MQ=255)
                                                                                                                                    aaTCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGat            >  1:735242/1‑143 (MQ=255)
                                                                                                                                      tCATGCGGGTTGCATCAGGGCGGGAAGTGAGTGAATCCTGGGGGGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGATGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGatat          <  2:563220/143‑1 (MQ=38)
                                                                                                                                        aTTGAGGGTGCAGCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATaa        <  2:329565/143‑1 (MQ=255)
                                                                                                                                            aGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGGGTAGACGTGTCAGGCCAGCTGGTCCCAGATATAATCGc    >  1:40982/1‑143 (MQ=255)
                                                                                                                                              gTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTg  >  1:614161/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GCGCAGGTTGTCGCACAGCTTCCACAGCTTCGCGACCAGATCGTTATTGTTCATTGTGAGTTCCGTAAATTAAGCAGCGGCCCAAATTCATCGAGCCGCAGAAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTG  >  NC_000913/4582995‑4583279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.