Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT32.4% 65.4 / 49.9 72intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (29/19);  new base T (11/12);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA  >  NC_000913/4295918‑4296200
                                                                                                                                              |                                                                                                                                            
tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGACTAAGCGGTTCAGTGAAGGCGTTCCCGCCGCATCCGACATCAAc                                                                                                                                              >  2:181962/1‑143 (MQ=11)
tAAGGCGTTTATGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                              <  2:639812/143‑1 (MQ=17)
    gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACCGCCt                                                                                                                                          >  1:291434/1‑143 (MQ=17)
     cGTTTATGCCGCATCCGACATTCACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGACAAGGCGTTTACGACGCATCCGCCATCACCGCCTg                                                                                                                                         >  2:889247/1‑143 (MQ=11)
       tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACAGCGTTTACGCCGCATCCGACCTCAATCCCTGAt                                                                                                                                       >  2:744678/1‑143 (MQ=11)
       tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATACGGCGCTTACGCCGCATCCGACATCAACGCCTTAt                                                                                                                                       >  2:499691/1‑143 (MQ=255)
        ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                      <  1:417933/143‑1 (MQ=32)
              cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAATGCCTCATCCGACGc                                                                                                                                >  1:668703/1‑143 (MQ=255)
                     gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                         >  2:507364/1‑143 (MQ=21)
                     gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTACGACGGATAACGCGTTTACGCCGCCTCCGACATCCCCCCCTGACGCCCCGCTTACcgc                                                                                                                         >  1:227606/1‑143 (MQ=11)
                       cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                       <  1:499691/143‑1 (MQ=21)
                       cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                       <  1:753033/143‑1 (MQ=38)
                        aTCACCGCCTGCTGCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                                                                      <  1:297988/143‑1 (MQ=255)
                        aTCAACGCCTGATGCGACGCTTACCGCGTCTCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                      >  2:316579/1‑143 (MQ=255)
                        aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATAAATGCCTGATGCGACGCTTGACGCGTc                                                                                                                      >  2:697032/1‑143 (MQ=12)
                        aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACACCGCAATAGCCTGATCTCGCGTGCTCTTGTAGGTCGGATCAGGCGCTTACGCCGCATCCGACATCAATGCCTGAGGCGCCGCCTGCCGCGTc                                                                                                                      >  2:44931/1‑143 (MQ=11)
                          tagcgcgTGATGCGACTCTTAACGCTTCGTATCCGGCCTACGCCAAACCGCTCAATGGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                    <  2:291434/140‑1 (MQ=11)
                          cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGTAAAGGCGTGTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCtc                                                                                                                    >  2:32744/1‑142 (MQ=255)
                          cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCGTGCTGCGACGCTTGCCGAGTCtt                                                                                                                    >  1:684009/1‑143 (MQ=255)
                            ccGCCTGATGCGACGCTCAACGCGTCGTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                  <  1:248926/142‑1 (MQ=255)
                            aCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                  <  1:313330/143‑1 (MQ=38)
                            aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                  <  1:697032/143‑1 (MQ=25)
                            aCGCCGGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                  <  1:94554/143‑1 (MQ=255)
                              gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                <  1:328355/143‑1 (MQ=39)
                                     gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                         <  1:493813/143‑2 (MQ=18)
                                     gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                         >  1:21912/1‑142 (MQ=21)
                                     gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                         <  1:32744/143‑2 (MQ=21)
                                      cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACACCGCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCCTACCAGGcgcatc                                                                                                        >  2:513675/1‑138 (MQ=11)
                                       gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTACGc                                                                                                       >  2:70235/1‑143 (MQ=255)
                                        aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCCTACGcc                                                                                                      >  2:742070/1‑143 (MQ=37)
                                             tAACGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa                                                                                                 <  1:355873/143‑1 (MQ=38)
                                               ccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCTAGACAgg                                                                                               >  2:72207/2‑142 (MQ=255)
                                                cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                    <  1:21834/105‑1 (MQ=18)
                                                cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg                                                                                                                                    >  2:21834/1‑105 (MQ=32)
                                                cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGgcg                                                                                              >  2:703738/1‑143 (MQ=255)
                                                   gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                                                                 <  2:344995/105‑1 (MQ=18)
                                                   gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                                                                 >  1:344995/1‑105 (MQ=33)
                                                   gTCTTAGCAGGCCGCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                           <  2:534588/143‑1 (MQ=255)
                                                    tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                     <  2:243412/100‑1 (MQ=18)
                                                    tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                     >  2:511606/1‑100 (MQ=34)
                                                    tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                     <  1:511606/100‑1 (MQ=34)
                                                    tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                     >  1:243412/1‑100 (MQ=33)
                                                     cTTATCGGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                         <  1:801578/143‑1 (MQ=38)
                                                      ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACCCCAGACAGCGCACTac                                                                                        >  2:493813/1‑142 (MQ=12)
                                                         tCAGGCCTACGCCAGGCCGCGCAACCGCCTGCTTTCGCGTGATTTTGTAGGTCGGATACGGCGTTTACGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                     <  2:755724/143‑1 (MQ=14)
                                                              ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGGTTGACGCGCCAGATCAGGACTACGCCAGGCAGCGCAAGAGCGTGAttg                                                                                >  2:97652/1‑142 (MQ=11)
                                                              ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATGGCCTGAttt                                                                                >  1:138946/1‑143 (MQ=38)
                                                                tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                          >  2:42761/1‑83 (MQ=25)
                                                                tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                          <  1:42761/83‑1 (MQ=25)
                                                                tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCCGCtttgc                                                                              >  1:680448/1‑141 (MQ=255)
                                                                 aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                             <  1:38043/143‑1 (MQ=39)
                                                                   gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                        <  1:825394/82‑1 (MQ=25)
                                                                   gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                        >  2:825394/1‑82 (MQ=35)
                                                                            cgcAATAGCGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                  <  1:541252/143‑1 (MQ=255)
                                                                             gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATACCCTGATTTAGCGTGCTCTTGTac                                                                 >  1:724874/1‑142 (MQ=255)
                                                                                             gCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                       <  1:612497/57‑1 (MQ=255)
                                                                                                   ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                                               <  2:262712/139‑1 (MQ=18)
                                                                                                   ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAt                                               >  1:262712/1‑139 (MQ=14)
                                                                                                         agttCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                     <  1:889247/140‑1 (MQ=38)
                                                                                                            tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                                                                    >  1:196121/1‑109 (MQ=255)
                                                                                                                     gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAGTAGCCTGATATAGCGTGATTTTGTAGGTCGGCTAGGGCGGTTACGCCGCATCCGGCATCACCGCCt                         >  2:453893/1‑143 (MQ=11)
                                                                                                                             gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATGCCGCACCAGCGCCTGAcgcgcc                 >  2:696376/1‑141 (MQ=11)
                                                                                                                              ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAGGCCTGTTGCGACg                >  2:131731/1‑143 (MQ=25)
                                                                                                                                gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGATAAGGCGATTACGCTGCATCCGACCTCAACGCCTGCCGCGACGCt              >  2:644384/1‑143 (MQ=11)
                                                                                                                                    ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCAt                                        >  1:815202/1‑113 (MQ=38)
                                                                                                                                    ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAt                                        <  2:815202/113‑1 (MQ=18)
                                                                                                                                     cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                             >  2:819520/1‑43 (MQ=40)
                                                                                                                                      gaaaTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGTGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc        <  1:735100/140‑1 (MQ=21)
                                                                                                                                      gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                             >  2:4025/1‑90 (MQ=40)
                                                                                                                                      gACATCAACGCCTGATGCGACGATTGCAAAGAGTGAGTAAGCGTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTACGCCGCATCCCACATCACAGCCCGACGCGACGCtgcccgc        >  2:489699/1‑137 (MQ=2)
                                                                                                                                       aCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATGTCAAACAGTGTTTTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATCCGACATGAACGCCTGCTGCGACGCGTGAcgcg       >  1:128710/1‑143 (MQ=11)
                                                                                                                                        cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt      <  1:97652/143‑1 (MQ=34)
                                                                                                                                            aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa  >  1:587358/1‑143 (MQ=32)
                                                                                                                                             aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTac                                               <  2:812645/97‑2 (MQ=17)
                                                                                                                                              |                                                                                                                                            
TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTA  >  NC_000913/4295918‑4296200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: