Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,196,305 C→T R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT95.7% 118.7 / NA 48R395C (CGT→TGT) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (30/15);  total (33/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196173‑1196444
                                                                                                                                    |                                                                                                                                           
gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGTCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGCGTGTCTgatgga                                                                                                                                   >  1:812776/1‑143 (MQ=255)
     tCCTGGCTCTATTTTTCTCTCCGCTGTGATGATGCTGCGACACATGGGTTGGACTGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                              <  1:697142/143‑1 (MQ=21)
     tCCTGGCTCTATTATTCTCTCCGCTGAGATTATGCTGCGATATATTTGTTCTACCAAAGCGGCTGACTAAAAGGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                              <  1:372092/143‑1 (MQ=21)
        tGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                           >  1:730585/1‑143 (MQ=255)
        tGGCTCTATTATTCTCTCCACTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                           >  1:136532/1‑143 (MQ=255)
            tctATTATTCTATCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGTAGCGGCTGACTTAATTGTTAAAGGTATGGCAGGCGCAATCTAGGGGGAAACGGTAACCTACGAGTTCGAGCGCACGATGGATAgcgcggacct                                                                                                                       >  2:857460/1‑137 (MQ=255)
              tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTCATTGTTAAAGGTATGGCAGGCGCAATCAACGCGAAAACCGTAACCTCTGACTTCGAGCGACTGATGGATGGCCCTaaccgcc                                                                                                                     >  2:89410/1‑138 (MQ=255)
              tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAGTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTGTGATGGATGGCGCTGAActgc                                                                                                                     >  1:433726/1‑143 (MQ=255)
                      tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAGACTGCTGAAATGt                                                                                                             >  2:637037/1‑143 (MQ=255)
                      tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGt                                                                                                             <  1:834317/143‑1 (MQ=255)
                      tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGt                                                                                                             >  2:461512/1‑143 (MQ=255)
                        tcCGCTGAGATGATGCTGCGCCACATGGGCTGGACCGAAGCGGCTGACTTAATTGTTCATGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTc                                                                                                           >  2:851593/1‑143 (MQ=255)
                         ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGTTct                                                                                                          >  1:544986/1‑142 (MQ=255)
                          cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTCAACTGCTAAAACGTTCag                                                                                                         >  2:2775/1‑143 (MQ=255)
                                 atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAATGTATGGAAGGCGATATAAACGCGACAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAATGGTTCTGAGgttcg                                                                                                  >  2:498584/1‑141 (MQ=255)
                                 atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATCCTCAGAGCTTgg                                                                                                  >  2:76179/1‑143 (MQ=255)
                                        tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGATAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                           >  2:527747/1‑143 (MQ=255)
                                          cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat                                                                                         >  2:72020/1‑143 (MQ=255)
                                            ccACATTGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTACCCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGTTCAGAGTTTGGTGACGCGatca                                                                                       >  2:829327/1‑143 (MQ=255)
                                             cacaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGGAACCTATGACTGCGAGTGGCTGATGTATGCCGCTAAACTGCGGAAATGTTCAGAGTTTGGTGACGCGatcat                                                                                      <  1:588491/143‑1 (MQ=18)
                                             cacaTGGGGTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcat                                                                                      <  1:31955/143‑1 (MQ=255)
                                               caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCg                                                                                    <  1:291912/143‑1 (MQ=255)
                                                  gggCTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAGGGCGCAATCAGCGCGAGAACCTTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa                                                                                 <  2:670864/143‑1 (MQ=18)
                                                   ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa                                                                                >  1:628060/1‑143 (MQ=255)
                                                           cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGACCGCGCTCCCGCTACGGCTGAAATGTTCAGCGTTTGGTGACGCGATCATCGAAAACACGTAAt                                                                        >  1:646804/1‑143 (MQ=18)
                                                                      aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg                                                             >  1:385127/1‑143 (MQ=255)
                                                                        gtAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACGTGTAATGCCGGAGTTTGtt                                                           >  1:701822/2‑143 (MQ=255)
                                                                         tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa                                                          >  1:754739/1‑143 (MQ=255)
                                                                              gTTACAGGTATTGAAGGCGCAATCAACGCGAAAGCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAttt                                                     <  2:639821/143‑1 (MQ=255)
                                                                               ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta                                                    >  1:781151/1‑143 (MQ=255)
                                                                               ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAAACGGAATGCCGTAGTTTGTTAAATtta                                                    >  2:140618/1‑143 (MQ=255)
                                                                                     gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACg                                              >  1:280623/1‑143 (MQ=255)
                                                                                       aTGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACggg                                            >  2:502484/1‑143 (MQ=255)
                                                                                         ggAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAg                                          >  2:586707/1‑143 (MQ=255)
                                                                                          gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGAGTCTGATGGATGACGCTAAACTGCTGAAATGTTCCGAGTCTGGTGACGCGATCATAGCAAACATGTAATCACGTAGGTTGTTAAATCTATTAACGGgcgc                                         >  2:584518/1‑143 (MQ=255)
                                                                                              gcgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTaa                                     <  1:179532/143‑1 (MQ=255)
                                                                                                gcAATCAACGTCAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                                   <  2:616688/143‑1 (MQ=255)
                                                                                                gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTGTGATTGATCGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                                   >  2:189901/1‑143 (MQ=255)
                                                                                                         gcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgggg                          >  1:109997/1‑140 (MQ=255)
                                                                                                           gaaattcGTAGCCTATGGCTGCGAGTGCCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgtt                        <  1:684425/137‑1 (MQ=25)
                                                                                                              aaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGCGCGTAACGCTCCCGTTGttttt                     >  1:55451/1‑143 (MQ=255)
                                                                                                               aCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                    <  1:72020/143‑1 (MQ=255)
                                                                                                                   tAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTa                >  1:147663/1‑143 (MQ=255)
                                                                                                                   tAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTa                <  2:730585/143‑1 (MQ=255)
                                                                                                                     aCCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg              <  1:851593/143‑1 (MQ=255)
                                                                                                                          tGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctgct         >  2:11616/1‑143 (MQ=255)
                                                                                                                                 gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  <  1:139375/143‑1 (MQ=255)
                                                                                                                                 gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  >  1:34727/1‑143 (MQ=255)
                                                                                                                                    |                                                                                                                                           
GTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196173‑1196444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: