Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,936,308	C→T	V3I (GTA→ATA)	zwf ←	glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,308	0	C	T	100.0%	185.9 / NA	59	V3I (GTA→ATA)	zwf	glucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAA  >  NC_000913/1936170‑1936447                                                                                                                                           |                                                                                                                                            cTACGCCGATAATCCGGATGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGcc                                                                                                                                         <  1:417447/143‑1 (MQ=255) cTACGCCGATAATCCGGATGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGcc                                                                                                                                         <  1:195579/143‑1 (MQ=255)        gATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACCGGCCTGGGCTGTTTGCGTTATCGCCagggcca                                                                                                                                  >  2:339999/1‑137 (MQ=255)        gATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCAt                                                                                                                                  >  2:253210/1‑143 (MQ=255)             tCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTcc                                                                                                                             <  1:495332/143‑1 (MQ=255)             tCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTAATAACGACAATTCAATAAATTATTACGCGTTGGTGCACCGCGCGCGCCGAAAATGAACAGGTCACGGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTcc                                                                                                                             >  1:886087/1‑143 (MQ=255)              ccGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGAGCTGTTTGCGTTATCGCCAGCTCATTGTCCt                                                                                                                            >  1:576977/1‑143 (MQ=255)                 ggTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTaa                                                                                                                         <  1:133684/143‑1 (MQ=255)                      ccGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaa                                                                                                                    >  1:851950/1‑143 (MQ=255)                         ggTTGAGCTGACCGGCTTTTTCCAGTCGATACACGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaacta                                                                                                                 <  1:28770/143‑1 (MQ=255)                          gTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTGAGTtaactaa                                                                                                                >  1:753644/1‑143 (MQ=255)                           ttGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTCAGTtaactaac                                                                                                               >  1:254123/1‑143 (MQ=255)                              aGCTGACCGGCTCGTTGCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCg                                                                                                            <  2:710736/143‑1 (MQ=255)                                cTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGt                                                                                                          >  1:180035/1‑143 (MQ=255)                                    ccGGCGTTTTCCAGTTGAGACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGTGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACtt                                                                                                      <  2:612415/143‑1 (MQ=255)                                     cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTa                                                                                                     >  1:310904/1‑143 (MQ=255)                                      ggCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTaa                                                                                                    >  1:507453/1‑143 (MQ=255)                                      cgcttcctCCAGTTGATACAGGGAAGGCAGCAATTTCCGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTaa                                                                                                    <  1:339999/136‑1 (MQ=255)                                          cttcccAGTTGATACAGCGAAGGCAGCAATCTACGACGCGCAAGGTCGCCCTTCGCGCCGAAAATGACCAGGTCACAGCCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCa                                                                                                <  1:673003/139‑1 (MQ=255)                                           tttCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCAAg                                                                                               >  2:851247/1‑143 (MQ=255)                                            ttCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAgg                                                                                              <  1:807844/143‑1 (MQ=255)                                              ccAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGGTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGt                                                                                            >  1:509678/1‑143 (MQ=255)                                                aGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGtat                                                                                          >  1:15958/1‑143 (MQ=255)                                                  ttGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGGCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTGAGTTACCTAACCCGCTCCTTAAGCCAGGGgatcc                                                                                        >  2:750614/1‑141 (MQ=255)                                                    gATACAGGGAAGGCAGCAATTGACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACtt                                                                                      <  1:437600/143‑1 (MQ=255)                                                      tACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGt                                                                                    <  1:172427/143‑1 (MQ=255)                                                             aaGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTCAGCCCGGGCGTCCTTGTCATTTTc                                                                             >  1:12571/1‑143 (MQ=255)                                                             aaGGCAGCAATTTACGACGCGCAAGGTCGCCTTGCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGGCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTc                                                                             <  2:162229/143‑1 (MQ=255)                                                             aaGGCAGCAAGGTACGACGCGCAAGGTCGCGTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTGGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTc                                                                             <  2:243214/143‑1 (MQ=255)                                                              cggCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCTTGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCt                                                                            <  1:374068/142‑1 (MQ=255)                                                                  agcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTTCCTTCTCGCCATGTCATTCTCCTTCAGTTAACTAACCCGGTCCTTAAGCCATGGAATACTTGTAATTTTCTTACg                                                                        >  2:603352/1‑143 (MQ=255)                                                                       tttACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGACTACTTGTAATTTTCTTACGGTGCa                                                                   >  2:715871/1‑143 (MQ=255)                                                                            gacgCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTa                                                              <  1:100413/143‑1 (MQ=255)                                                                                 gcAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCt                                                         >  2:488858/1‑143 (MQ=255)                                                                                   aaGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCttt                                                       >  1:523449/1‑143 (MQ=255)                                                                                    aGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATCCTCCTTAAGTTAACTAACCCGGTACTTAAGCAAGGGTATACTTGTCATTTTCTTACGGTGCACTGTACTGCtttt                                                      <  1:655931/143‑1 (MQ=255)                                                                                      gTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTAc                                                    >  2:129018/1‑143 (MQ=255)                                                                                      gTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTAc                                                    <  2:180035/143‑1 (MQ=255)                                                                                       tCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACg                                                   >  2:60894/1‑143 (MQ=255)                                                                                       tCGCCTTTCGCGCCGAAAATGACCACGTCACAGTCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACg                                                   <  1:28260/143‑1 (MQ=255)                                                                                       tCGCCTTCCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATTGCCATGTCATTCTCCTTAAGTTATCTAACCCGGTACGTAAGCCAGAGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACg                                                   <  2:14710/143‑1 (MQ=255)                                                                                             ttCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTg                                             >  2:156961/1‑143 (MQ=255)                                                                                                   ccGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaaaa                                       <  2:843174/143‑1 (MQ=255)                                                                                                   ccGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGCTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaaaa                                       <  1:448775/143‑1 (MQ=255)                                                                                                   ccGAAAATGACCAGCTCACAGGCCTTGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaaaa                                       <  2:3243/143‑1 (MQ=255)                                                                                                       aaaTGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGt                                   >  2:111791/1‑143 (MQ=255)                                                                                                          tGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTGACTAACCCGGTACTTACGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTGACGCGCTTGACAAAAcgcgaaa                                >  2:490952/1‑137 (MQ=255)                                                                                                           gACCAGGTCACAGGCCTGGGCTGCTTCCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAAc                               <  1:633346/143‑1 (MQ=255)                                                                                                             ccAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAATGc                             <  2:851950/143‑1 (MQ=255)                                                                                                              cAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCt                            >  1:436292/1‑143 (MQ=255)                                                                                                                ggTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTa                          <  1:458113/143‑1 (MQ=255)                                                                                                                 gccacaGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATCCTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTAt                         <  1:40114/141‑1 (MQ=255)                                                                                                                        gCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTCCTTAAGCCAGGGTATACTTGGAATTTGCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCg                  >  1:166267/1‑143 (MQ=255)                                                                                                                          cTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGTACACGAATTACGCTTATCCACCCGTg                >  2:346985/1‑143 (MQ=255)                                                                                                                           tGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGc               <  2:841419/143‑1 (MQ=255)                                                                                                                             ggCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGa             >  1:629449/1‑143 (MQ=255)                                                                                                                                  tttGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCCGGGTATACTTGTACTTTTCTTACGGTGCACTGTACTGATTTTACGCGCTTGCGAAAACTGTAAACGCTTATCCAGCGGTGCGCTTACg        >  1:352854/1‑143 (MQ=255)                                                                                                                                  gttGCGTTATCGCCATGTCATTCTCCTTAGGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACg        <  2:640265/142‑1 (MQ=255)                                                                                                                                        ttATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGaaaa  <  1:71018/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAA  >  NC_000913/1936170‑1936447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑