Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,903 IS2 (–) +5 bp noncoding (5‑9/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4049903 =0 (0.000)65 (1.150) 56/284 0.0 100% noncoding (5/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 40499070 (0.000)71 (1.260) 55/284 0.0 100% noncoding (9/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

Only 100 of 134 total aligned reads displayed.

TGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTA  >  NC_000913/4049761‑4050045
                                                                                                                                               |                                                                                                                                              
tGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAgg                                                                                                                                                >  2:231052/1‑143 (MQ=255)
tGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTCTGAAAAGGCTATTAGAAATTACTATGCAACCACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTAAGCCGCGCAgg                                                                                                                                                >  2:554541/1‑143 (MQ=255)
 ttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggcgcttcaggtggccagtctag                                                                                                                                                <  1:834087‑M1/1‑1 (MQ=255)
 ttcctgtgtggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctag                                                                                                                                                >  1:725621‑M1/143‑143 (MQ=255)
     tgtggggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTAc                                                                                                                                            <  1:586689‑M1/5‑1 (MQ=255)
      gtgtggtacgccgtctgcgtttctccggacctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtatggagattcagagggcaagcctaGGTACa                                                                                                                                           <  2:328483‑M1/6‑1 (MQ=255)
       gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtgg                                                                                                                                         >  2:121998‑M1/1‑140 (MQ=255)
       gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtgg                                                                                                                                         <  2:418840‑M1/143‑4 (MQ=255)
        gtggtacgccgtctgcgttgctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACaga                                                                                                                                         >  1:395418‑M1/136‑143 (MQ=255)
         ttgttttccgAAGTAAGTAATATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGAAACAACTGAAAAAAAAGTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggat                                                                                                                                       <  2:316843‑M1/140‑6 (MQ=255)
         tggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcactgatttgagtgatattggttgtcgggaggttaagggggccagtctgGGTACagag                                                                                                                                        <  1:538411‑M1/9‑1 (MQ=255)
         cTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTTTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAACAGTAAAGTTAGTCGCGTCGGGTACtggat                                                                                                                                       >  2:329150‑M1/1‑138 (MQ=255)
          ttttttCGTAAGTAAGCACCGTAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggaat                                                                                                                                      >  2:529413‑M1/1‑137 (MQ=255)
           tttttCGCAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggattt                                                                                                                                     <  1:377489‑M1/143‑8 (MQ=255)
             tttCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgc                                                                                                                                   <  2:894711‑M1/143‑10 (MQ=255)
               tCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgccc                                                                                                                                 <  1:217620‑M1/143‑12 (MQ=255)
               gccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGa                                                                                                                                  <  1:816816‑M1/15‑1 (MQ=255)
               ccgTAAGTAAGCTACCTAAGGTGTCGCGTTTTGTGATGCCTATTACAAATTCTTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgccc                                                                                                                                 <  2:552272‑M1/142‑12 (MQ=255)
                 gtaagtaaGCAACATAAGCTGTCACGGTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGGACTGAACACACAAGCGGCAAGTTTGTCGCGGTGGGTTTtggatttgcccct                                                                                                                               >  1:351702‑M1/1‑130 (MQ=255)
                     tgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCt                                                                                                                            <  1:108974‑M1/21‑1 (MQ=255)
                        agCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTAACCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggattagcgcctatacttc                                                                                                                        >  2:878032‑M1/1‑123 (MQ=255)
                         tttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaagtagtattaagactatcacttatttaagtgatattggttgtctggggattcagggggccagtctaGGTACAGAGGTGAGAGGTTCTATCt                                                                                                                        <  1:438594‑M1/25‑1 (MQ=255)
                         gCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcc                                                                                                                       >  1:916909‑M1/1‑122 (MQ=255)
                          cAAGATAAGCTGTCACGGTTTGTGATGGCTATTAGAAATTCATATGCAACAAATGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcca                                                                                                                      <  2:478647‑M1/143‑23 (MQ=255)
                          cAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcca                                                                                                                      <  1:268841‑M1/143‑23 (MQ=255)
                          cAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcca                                                                                                                      <  1:100082‑M1/143‑23 (MQ=255)
                           tctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCttt                                                                                                                      >  1:994273‑M1/117‑143 (MQ=255)
                             cATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAAATTACAAAAAGTGCTTTCGGAACTGAACAAAACAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccccatagttccagg                                                                                                                    >  2:489088‑M1/1‑119 (MQ=255)
                               cggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggcgattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGa                                                                                                                  <  2:346824‑M1/31‑1 (MQ=255)
                                  ccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCt                                                                                                               >  2:944523‑M1/110‑143 (MQ=255)
                                   cctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCtt                                                                                                              >  1:500284‑M1/109‑143 (MQ=255)
                                    ctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagagggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCttt                                                                                                             <  2:965847‑M1/36‑1 (MQ=255)
                                     gTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgtta                                                                                                           <  1:880700‑M1/143‑34 (MQ=255)
                                      tCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcccgacatctgttac                                                                                                          >  2:35906‑M1/1‑109 (MQ=255)
                                       agacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTAc                                                                                                          <  1:207171‑M1/39‑1 (MQ=255)
                                        aCGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatca                                                                                                        >  1:301667‑M1/1‑107 (MQ=255)
                                         acatcaatcagcagttcgcctatgactagtctaaacgctaggattaagactatcacttatccgagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTGCAGACCTTTTACtt                                                                                                        >  2:269349‑M1/103‑143 (MQ=255)
                                          gTTTTGTGATGGCTAATAGAAATTCCTAAGCAAGAACTTAAAAAAAAGTACAATAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcact                                                                                                      <  2:209399‑M1/143‑39 (MQ=255)
                                             caatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACg                                                                                                    >  2:688618‑M1/99‑143 (MQ=255)
                                             caatcatctgttctccaatgactaggctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACg                                                                                                    >  1:875683‑M1/99‑143 (MQ=255)
                                               tgtatggCTATTAGAAATTCATAGGAAACAGTTGGGCACAACTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacc                                                                                                 <  2:714422‑M1/140‑44 (MQ=255)
                                               gtgATGGCTAGTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacc                                                                                                 <  1:250358‑M1/143‑44 (MQ=255)
                                                tcatctgttctccaatgactagtctaaaaactagtattaagcctatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGCGGTAAGATGTTCTATCTTTCAGACCTTTTCCTTCACGtag                                                                                                 <  1:964797‑M1/48‑2 (MQ=255)
                                                tcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggggattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTaa                                                                                                 <  1:765133‑M1/48‑1 (MQ=255)
                                                  aTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGGACGAAAAATAGTAAAGTTAGTCGCTTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccat                                                                                              <  1:469876‑M1/143‑47 (MQ=255)
                                                     gCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAATAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcacctatatttccagacatctgttatcacttaaccaattac                                                                                           >  2:493004‑M1/1‑94 (MQ=255)
                                                      gttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAt                                                                                           <  1:731918‑M1/54‑1 (MQ=255)
                                                        tctccaatgactagtctgaaagcgagtattgagactatcccttatttaagtgatattggttgtctggagtttcaggcggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAttt                                                                                         >  2:907047‑M1/88‑143 (MQ=255)
                                                         ctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTg                                                                                        <  1:469116‑M1/57‑1 (MQ=255)
                                                          tccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTATTACTTAGCGTAATCGGATTTgg                                                                                       <  2:34600‑M1/58‑1 (MQ=255)
                                                          tAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcc                                                                                      >  2:479754‑M1/1‑89 (MQ=255)
                                                            gAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattccaagcccg                                                                                    >  1:398058‑M1/1‑87 (MQ=255)
                                                               aTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTATTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctg                                                                                 <  1:547343‑M1/143‑60 (MQ=255)
                                                                    tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAGGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaaccccgctgccgcg                                                                            >  1:329844‑M1/1‑79 (MQ=255)
                                                                    agtctaaaaactcgtattaagactatcacttagttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGCCCTTTTACTTCACGTAATCCGGTTTGGCTGTATAttt                                                                             <  2:28140‑M1/68‑1 (MQ=255)
                                                                    agtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAttt                                                                             >  2:406500‑M1/76‑143 (MQ=255)
                                                                     gagcaACAACTGAAAAAAAATTACAACAAGGGCTTGCTGAACTGAACAAAACAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcag                                                                           <  2:207161‑M1/141‑66 (MQ=255)
                                                                      tctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                           <  1:949211‑M1/70‑1 (MQ=255)
                                                                      tctaaaaactagcattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                           >  1:701971‑M1/74‑143 (MQ=255)
                                                                           caacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattc                                                                     <  1:755446‑M1/143‑72 (MQ=255)
                                                                            aactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgcc                                                                     <  1:296123‑M1/76‑1 (MQ=255)
                                                                            aacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGCGTAAAGTTAGGGGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcc                                                                    >  1:279754‑M1/1‑71 (MQ=255)
                                                                                gAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtg                                                                <  2:139773‑M1/143‑77 (MQ=255)
                                                                                gAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgaggatactcccgtg                                                                >  2:749777‑M1/1‑67 (MQ=255)
                                                                                 gtgttaggactgtcgcttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagt                                                                >  2:44740‑M1/63‑143 (MQ=255)
                                                                                  aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtgg                                                               >  2:393023‑M1/1‑66 (MQ=255)
                                                                                    ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtcag                                                             <  1:713252‑M1/84‑1 (MQ=255)
                                                                                       aaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgcccccgatattcccgtggcgagc                                                          >  1:378328‑M1/1‑61 (MQ=255)
                                                                                        gaccatgacttatttgagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAt                                                         >  1:363949‑M1/56‑143 (MQ=255)
                                                                                            atcacttatttaagtcatattggttgtctggagattcagggggccagtctaCGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACt                                                     >  1:452276‑M1/52‑143 (MQ=255)
                                                                                             tcacttatttaagggatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTactgactg                                                    >  1:244831‑M1/51‑143 (MQ=255)
                                                                                              aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacc                                                   >  2:570621‑M1/1‑54 (MQ=255)
                                                                                                cttatttaagttattatggttgtctggagattccgggggccagtctaGGTATAGATGTAAGACGACCTATCGTTCAGACCGTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgggg                                                 >  2:486994‑M1/48‑143 (MQ=255)
                                                                                                 aaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccaggcatctgttatcacttaaccaattattagcgttctgccgcagatattcccgtggcgagcgataacccag                                                >  1:85005‑M1/1‑51 (MQ=255)
                                                                                                   atttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGt                                              <  1:310701‑M1/99‑1 (MQ=255)
                                                                                                       tttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataaccccgcgacct                                          >  1:473085‑M1/1‑45 (MQ=255)
                                                                                                        ttCTGAGCTGAACATAAAAGAGTAAAGTTAGTCGCCTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcacta                                         <  2:591059‑M1/143‑100 (MQ=255)
                                                                                                         gtgatatccgtcgtctggagatgcaggtggtcagtctaGGGACAGAGGTAAGTTGTTCTATCTTTCAGACCTTTCACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTa                                        >  2:317575‑M1/39‑143 (MQ=255)
                                                                                                           tgaactgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgc                                      <  1:668966‑M1/143‑103 (MQ=255)
                                                                                                           gatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAtt                                      <  2:451938‑M1/107‑1 (MQ=255)
                                                                                                             tattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGCCTGAATATTCTATCCGTCTCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                                    >  1:966240‑M1/35‑143 (MQ=255)
                                                                                                               ctgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggat                                  <  1:121998‑M1/143‑107 (MQ=255)
                                                                                                               ctgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaaccaattacaagcccgctgccgcagatcttcccgtggcgagcgataaccccacgcactatgcggac                                  >  2:260518‑M1/1‑37 (MQ=255)
                                                                                                                  aacAAAAAAGAGTAAAGTTAGTCGCGTGGGGTACtggatttgcccctatattcccagacatctgttatcactcaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgcc                               <  2:688983‑M1/143‑110 (MQ=255)
                                                                                                                  aacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcaagcgataacccagcgcactatgcggatgcc                               >  1:646589‑M1/1‑34 (MQ=255)
                                                                                                                   ttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaa                              >  1:434010‑M1/29‑143 (MQ=255)
                                                                                                                       aaaaGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagctattcccgtggcgagcgataaccccgcgcactacgcggacgcccttcg                          >  2:77686‑M1/1‑29 (MQ=255)
                                                                                                                        tggggattccgggggccagcctaGGTACAGAGGTGAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                         >  2:270136‑M1/24‑143 (MQ=255)
                                                                                                                        tggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTGACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                         >  2:498402‑M1/24‑143 (MQ=255)
                                                                                                                           agattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAt                      >  1:418604‑M1/21‑143 (MQ=255)
                                                                                                                            agTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactgtgcggatgccactcgttata                     >  1:784925‑M1/1‑24 (MQ=255)
                                                                                                                              ttcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGCCCTTTTACTTCACGTAATCGGAGTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCg                   >  1:69794‑M1/18‑143 (MQ=255)
                                                                                                                              tAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataccccagcgcaccacgcggctgcccttcgctacaat                   >  2:659882‑M1/1‑22 (MQ=255)
                                                                                                                               aacgtgagtcgcgTAGGGTACtggatttgcccctatatttccagacatctgttatcacttagcccattaccagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatg                  <  2:764142‑M1/137‑123 (MQ=255)
                                                                                                                                  gggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAAt               >  1:191399‑M1/14‑143 (MQ=255)
                                                                                                                                    gggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGc             >  1:90949‑M1/12‑143 (MQ=255)
                                                                                                                                     aGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaa            <  1:886889‑M1/143‑129 (MQ=255)
                                                                                                                                            ctaGGGACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATgcg     >  1:51080‑M1/4‑143 (MQ=255)
                                                                                                                                              aGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCt   >  2:469116‑M1/2‑143 (MQ=255)
                                                                                                                                               ggTACAGAGGTAGGATGTTCTATCTTTCACTCCTCGTACCTCACGTAGTCGGCTTCGGCTGAATATTTCGGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTCTAGTACGAAAGAAAAGATCCGTAATGCCTGATGCGCTa  <  2:258326/143‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
TGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTA  >  NC_000913/4049761‑4050045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.