BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,293,716	T→G	9.3%	V106G (GTA→GGA)	lpoA →	OM lipoprotein stimulator of MrcA transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,293,716	0	T	G	9.3%	464.4 / 2.1	173	V106G (GTA→GGA)	lpoA	OM lipoprotein stimulator of MrcA transpeptidase
Reads supporting (aligned to +/- strand): ref base T (60/87); new base G (24/2); total (84/89)
Fisher's exact test for biased strand distribution p-value = 6.38e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TGTGGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCA  >  NC_000913/3293478‑3293949

tgtgGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑t                                                                                                                                                                                                                                              <  2:39370/239‑1 (MQ=255)

tgtgGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAG‑AGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑Ta                                                                                                                                                                                                                                             <  2:826398/239‑1 (MQ=255)

 gtgGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑Ta                                                                                                                                                                                                                                             <  2:75956/239‑1 (MQ=255)

                ccGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGgcg                                                                                                                                                                                                                              <  1:244256/239‑1 (MQ=255)

                ccGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCATTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGgcg                                                                                                                                                                                                                              <  2:956573/239‑1 (MQ=255)

                  gATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGAGAGAGAA‑AACTGACCGCGG‑TAGGGATTAAACTGGCGCa                                                                                                                                                                                                                            >  1:678741/1‑239 (MQ=255)

                  gATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGACGCTCAGCGTTGCGAGAAAA‑AACTGCTGGCGG‑TAGAGATTAAACTGGGGCa                                                                                                                                                                                                                            >  1:1000731/1‑239 (MQ=255)

                   aTCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAg                                                                                                                                                                                                                           >  2:473583/1‑239 (MQ=255)

                    tCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGa                                                                                                                                                                                                                          <  1:22021/239‑1 (MQ=255)

                        tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCCAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGGGATTAAACTGGCGCAGAAAGa                                                                                                                                                                                                                      >  2:1104427/1‑239 (MQ=255)

                        tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACCGCTGGCGG‑TAGAGATTAAAACGGGGCGGAAAgg                                                                                                                                                                                                                      >  2:54503/1‑238 (MQ=255)

                                  atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGgc                                                                                                                                                                                                            >  1:824536/1‑239 (MQ=255)

                                  atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGAATTTGCTGgc                                                                                                                                                                                                            >  1:132528/1‑239 (MQ=255)

                                    atGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACAGGCCCAGAAAGATTTTGCTGgcgc                                                                                                                                                                                                          >  2:1068932/1‑239 (MQ=255)

                                     tGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGgcgcg                                                                                                                                                                                                         <  1:473583/239‑1 (MQ=255)

                                     tGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGgcgcg                                                                                                                                                                                                         <  1:582232/239‑1 (MQ=255)

                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGGGTTGTTTAAACAACTAC‑‑CGCAAAAACTGAACAATGTTCACAGTCGCGAGAAAA‑CAATGCTGGCGGTTAGGGTTTAAACTGGGGTGGAACG‑TTTTTCTGGGGCGCaaa                                                                                                                                                                                                     >  2:54376/1‑239 (MQ=255)

                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCaaa                                                                                                                                                                                                     >  2:20988/1‑239 (MQ=255)

                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAAATGGCGCAGAAAGATTTTGCTGGCTCGCcaa                                                                                                                                                                                                     >  1:789343/1‑239 (MQ=255)

                                         gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAAAACTGAACGATGCGCAGCGTCGCGGGAAAA‑CACTGCTGGCGG‑GAGAGGATAAACTGGCGACGAAAGATTTTGCTGGCGCGCcaa                                                                                                                                                                                                     >  1:972991/1‑239 (MQ=255)

                                          ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAAGGATTAAACTGGCGCAGAAAGATTTTTCTGgccgggaaaa                                                                                                                                                                                                    >  2:887862/1‑231 (MQ=255)

                                              cGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACGGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTg                                                                                                                                                                                                <  1:779651/239‑1 (MQ=255)

                                                    aGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGc                                                                                                                                                                                           <  2:680142/238‑1 (MQ=255)

                                                    aGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGc                                                                                                                                                                                           <  1:476489/238‑1 (MQ=255)

                                                       cTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                                                                                                                                                                                          <  2:1324465/236‑1 (MQ=255)

                                                       cTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                                                                                                                                                                                          <  2:1402281/236‑1 (MQ=255)

                                                                  ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                            <  2:43250/239‑1 (MQ=255)

                                                                  ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                            <  1:146050/239‑1 (MQ=255)

                                                                  ttttATCTCCAGCATATGCAGCAAATCTCGGATTACACCAGGATCAACTGGCAATTTCTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCACCTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAAAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAAAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                            <  2:1122073/239‑1 (MQ=255)

                                                                       gctgcagcaaAGGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGAtt                                                                                                                                                                       <  2:752136/235‑1 (MQ=255)

                                                                            agcagATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACCGGCGCAGAAAGATTTTGCTTGCGCGCAAAAATTGCTGGCGAAAATTACAACTGCCGATTTAGaa                                                                                                                                                                  >  1:1185324/1‑239 (MQ=255)

                                                                               agATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGaa                                                                                                                                                                  <  2:1045187/236‑1 (MQ=255)

                                                                                 aTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTTCTGGCGCGCCAAACTTGCTGGCGAAAATCACACCTGCCGATTTTGAaaaaaa                                                                                                                                                             >  2:432602/1‑239 (MQ=255)

                                                                                  tGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAAACTGCGCAGGAAGATTTTGCTGGCGCGCAAAACTTGCTGGCCAAAAACACACCCGCCGATTTTGAAcctgt                                                                                                                                                             >  2:809811/1‑234 (MQ=255)

                                                                                   gcagcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAAATGGAGCAGAAAGAATTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCCATTTAAAACAAAAcc                                                                                                                                                           >  2:1061580/1‑239 (MQ=255)

                                                                                      gcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCCCGCAAAACTTGCTGGCGAAAATCACAACTGCCGATTTAGAACAAAACC‑AGc                                                                                                                                                       >  1:167073/1‑239 (MQ=255)

                                                                                            ctctGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CCCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGAATTTGTGGGAGCGCAAAACTTTCTGGCGAAAAACACACCTGCCCATTTTAAACAAAACC‑CGCAAgcac                                                                                                                                                 >  2:9108/1‑237 (MQ=255)

                                                                                             tctGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGcg‑cg                                                                                                                                               <  1:1252745/239‑1 (MQ=255)

                                                                                               tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACAGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCCGGCGG‑GAGAGGTTAAACTGGCGCCGAAAAATTTTGCCGGGGCGCCAAACTTGCTGGCGGAAATCACACCTGGCGATTTTGAACACAACC‑AGCAAGCG‑CGtt                                                                                                                                             >  2:166547/1‑239 (MQ=255)

                                                                                                     tACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTgg                                                                                                                                        <  1:506855/238‑1 (MQ=255)

                                                                                                           gATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCACCTAC‑‑CGCAAGCACTGAACGATGCCCAGCGTCCCGAGAAAA‑CACTGCTCGCGG‑TTGAGGTTTAACCGGGGCCAAAACGTTTTGCTGGCGCGCCAAACTTTCTTGCGGCAATTACAACCGCCGGTTTTTGACAAAACC‑AGCAAGCG‑CGTTTCtggct‑ggcca                                                                                                                                >  2:102373/1‑237 (MQ=255)

                                                                                                               aaCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAAt                                                                                                                            <  2:1217640/239‑1 (MQ=255)

                                                                                                               aaCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTTCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCCGCCGATTTAGAACAAAACC‑AGCAAACG‑CGGTAATTGCA‑AGCcaaaa                                                                                                                             >  2:642614/1‑238 (MQ=255)

                                                                                                               aaCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAAATTTTGCTGGCGCGCCAAACTTGCTGGCGAAAATCACAACTTCCGATTTAGAACAAAACCAAGCAA‑CC‑CGTTACTGGCA‑GGCAAAAAt                                                                                                                            >  2:894185/1‑239 (MQ=255)

                                                                                                               aaCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAAATGGCGCAGAAAGATTTTTCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAAt                                                                                                                            >  2:1267956/1‑239 (MQ=255)

                                                                                                                aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTTACCAACTAA‑‑CCCAAGAACTGAACGAGGCCCAGCGGCGCGAGAAAA‑CACAGCTGGCGG‑TAGAGATTAAACTGGCGCGCAAAAACTTTGCTAGCGTGCAAAGATTGGTTGCGCAAATCACACCTTCCGATTTTGAAAAAAACC‑ATCAAGTG‑CGTTACTGGCG‑TGCaaaaaa                                                                                                                            >  2:1012043/1‑237 (MQ=255)

                                                                                                                aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTCCCGATTTAGAACAAAACC‑AGCAAACG‑CGTTACTGGCA‑AGCaaaaaac                                                                                                                           >  2:1046825/1‑237 (MQ=255)

                                                                                                                 cTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTGCTTGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGGTTTAGAACAAAACC‑AGCAAACG‑CGTTACTGGCAGGGCaaaaacg                                                                                                                           >  2:155041/1‑237 (MQ=255)

                                                                                                                    gCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAA‑CACTGCTGGCGG‑TAGAGGTTAAACATGCGCAGAAAAAATTTTCTTGCCCGCAAAACCTTTTGGCGGAAATCACAACTGCCGATTTAGAACCAAAAC‑AGGAAGAGCCGTCAC‑GGCA‑GGCAAAAAACGaag                                                                                                                       >  2:758126/1‑237 (MQ=255)

                                                                                                                    gCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAAACACACCTGCCGATTTAGAACCACAAC‑AGCAAGCG‑CGTAACTGGCC‑GGCAAAAAACGaa                                                                                                                        >  2:367044/1‑237 (MQ=255)

                                                                                                                     cAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATg                                                                                                                       <  1:1160435/238‑1 (MQ=255)

                                                                                                                     cAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATg                                                                                                                       <  2:333002/238‑1 (MQ=255)

                                                                                                                     cAATTACTCGCACTTCGGGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTAAACCAACTAC‑‑CGCAAGAACTGAACGATGTGCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTGGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAATCC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATg                                                                                                                       <  1:657236/238‑1 (MQ=255)

                                                                                                                        ttACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAAATGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACCGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGTTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGGTACTGGCC‑GGCGAAAAACGATgcca                                                                                                                    >  2:441545/1‑238 (MQ=255)

                                                                                                                                       gCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAGGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTc                                                                                                    <  1:889086/239‑1 (MQ=255)

                                                                                                                                       gCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTc                                                                                                    <  1:359572/239‑1 (MQ=255)

                                                                                                                                         atgggTGAAAGAAGGGCAAACCGGGCAGGCGGTTGAGTTTTTTAACCAACTAC‑‑CGCAAGAACGGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                                                  <  2:318134/236‑1 (MQ=255)

                                                                                                                                         aCTGGTGAAAGAAGGTAAAACGGGGCAGGCGGTTGAGGTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                                                  <  2:723665/239‑1 (MQ=255)

                                                                                                                                          cTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCCGGAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAAACACACCCGCCGATTTTAAAAAAAACC‑ACCAAGAG‑CGGTACTGGCAGGGCAAAAAACAAGCCTCGCCAGGGGGGCCCCTCCa                                                                                                  >  2:1165523/1‑239 (MQ=255)

                                                                                                                                             gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGGGCGCAAAACTTGCTGGCGAAAATCACACCTTCCGATTTAGAACAAAACC‑AGCAAACG‑CGGTACTGGCC‑GGCAAAAAAAGCTAGCAGCCAGGGGGGTCCCTCCact                                                                                                >  2:473118/1‑237 (MQ=255)

                                                                                                                                                    aagGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgc                                                                                      <  1:1115717/239‑1 (MQ=255)

                                                                                                                                                     agGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTg                                                                                       <  2:108907/238‑1 (MQ=255)

                                                                                                                                                       gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAAAC‑AGCAAGCG‑CGTTACTGGGA‑GGCAAAAAACGATGCCACCCAGGGGCGTCCCTCCATTGGTTTAATgcgc                                                                                    >  2:1239333/1‑239 (MQ=255)

                                                                                                                                                       gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAAATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGGTTTAGAAAAAAACC‑AGCAAACG‑CGTTACTGGCA‑GGCAAAAATAGAAGCCAGACAAGGGCGCCCTTCCACTGGTTTAATgggc                                                                                    >  1:1152082/1‑239 (MQ=255)

                                                                                                                                                        tAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcgc                                                                                    <  1:977487/238‑1 (MQ=255)

                                                                                                                                                        tAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                                                     <  1:610141/237‑1 (MQ=255)

                                                                                                                                                        tAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                                                     <  1:384187/237‑1 (MQ=255)

                                                                                                                                                              cGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTaa                                                                             <  1:1046825/239‑1 (MQ=255)

                                                                                                                                                               gggCAGGCGGTTGAGTTGTTAAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCTTCGCGAGAAAA‑CACTTCTGGCGG‑TAGAGATTAAACGGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGTCGAAAATCCCACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt                                                                            <  2:517144/239‑1 (MQ=255)

                                                                                                                                                               gggCAGAGGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACGGAACGATGCTCAGCGCCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTGGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt                                                                            <  1:1310711/239‑1 (MQ=255)

                                                                                                                                                                ggCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑GAGAGATTAAAATGGCGCAGAAAGATTTTGCTGGCGCGCAAAAATTGCTGGCGAAAATCACACCCGCCGATTTAGAACAAAAAC‑AGCAAACG‑CGTTAACGGCA‑CGCAAAAAACACAGCCCGCCAGGGGCGCCCCTTCCTTTCTTTATAGCGCGCGTTAAtt                                                                           >  2:977487/1‑239 (MQ=255)

                                                                                                                                                                   aGGCGGTTGTGTTTTTTAACCAACTAC‑‑CGCAAGACCTAAAGGATGCCCAG‑GGCGCGGGAAAACCCCTGCGGGCGG‑TAGAGATAAACCGGGCGAAGAAAAATTTTGCGGGCGCGAAAACCTTGCTGGCGAAAACCACCCCTGCCGATTTAGAAAAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGc                                                                         <  2:1176121/238‑1 (MQ=255)

                                                                                                                                                                                   tAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTTCTCAGGAACCGCTGCTTg                                                        >  1:390702/1‑239 (MQ=255)

                                                                                                                                                                                   tAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGAAGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTg                                                        >  1:559632/1‑239 (MQ=255)

                                                                                                                                                                                           tAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGc                                                                                                                                                                                           <  1:537677/103‑1 (MQ=255)

                                                                                                                                                                                           tAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGc                                                                                                                                                                                           >  2:537677/1‑103 (MQ=255)

                                                                                                                                                                                                cGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaagaa                                             <  1:862954/239‑1 (MQ=255)

                                                                                                                                                                                                 gCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaagaaa                                            <  2:824536/239‑1 (MQ=255)

                                                                                                                                                                                                   aaGAACTGAACGATGCTCAGCGTCGCGAAAAAG‑CCCTGCTGGCGG‑TAGAGATTAAACGGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa                                          <  2:346837/239‑1 (MQ=255)

                                                                                                                                                                                                       acggAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag                                       <  1:642614/235‑1 (MQ=255)

                                                                                                                                                                                                         tGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag                                    <  1:54503/239‑1 (MQ=255)

                                                                                                                                                                                                          gAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGa                                   <  1:1069473/239‑1 (MQ=255)

                                                                                                                                                                                                           aaCGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGaa                                  >  1:877481/1‑239 (MQ=255)

                                                                                                                                                                                                                tGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTgg                                                                                                                                        <  2:574956/133‑1 (MQ=255)

                                                                                                                                                                                                                tGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTg                             >  1:408264/1‑239 (MQ=255)

                                                                                                                                                                                                                tGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGCCCTTCCCTTGATTTACTGCGCGCGTTAATTGCTCAGGGACCGCTGCTTGGCGCGAAAGAAAAAAAGCAGAATAtta                             >  2:1150840/1‑238 (MQ=255)

                                                                                                                                                                                                                   tCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGAt                           <  1:731877/238‑1 (MQ=255)

                                                                                                                                                                                                                   ccAGCGTCGCGGGAAAC‑CCTGGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCACAAAACTTGCGGGGGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGAt                           <  2:873837/237‑1 (MQ=255)

                                                                                                                                                                                                                     aGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                                                  <  2:462702/165‑1 (MQ=255)

                                                                                                                                                                                                                     aGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGcc                        >  2:125900/1‑239 (MQ=255)

                                                                                                                                                                                                                     aGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGGTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGcc                        >  1:1391941/1‑239 (MQ=255)

                                                                                                                                                                                                                     aGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGACCTTCCa                                                                                                  >  1:462702/1‑165 (MQ=255)

                                                                                                                                                                                                                          cgcgAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa                                          >  1:803809/1‑216 (MQ=255)

                                                                                                                                                                                                                               gaAAA‑CCCTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCGGGCCCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAg               <  2:680639/238‑1 (MQ=255)

                                                                                                                                                                                                                                   a‑cacTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGct          <  1:1239333/239‑1 (MQ=255)

                                                                                                                                                                                                                                     cacTGCTGGCGG‑GAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc         <  1:760645/239‑1 (MQ=255)

                                                                                                                                                                                                                                     cacGGCCGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCGGGCGCACAAAACTTGATGGCGAAAATCCCCCCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc         <  2:789343/239‑1 (MQ=255)

                                                                                                                                                                                                                                       ctgctgGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCtctc       <  1:1193782/239‑1 (MQ=255)

                                                                                                                                                                                                                                            ggCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCa  <  1:441545/239‑1 (MQ=255)

                                                                                                                                                                                                                                                  tAGAGAGTAAACTGGCGCAGAAAGATTTGGGTGGCGCGCAAAACTTGCTGGGGAAAATCACACCTGCAGATTTAGAACAAAATC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag                                       <  1:324452/197‑1 (MQ=255)

TGTGGCACCCATACTCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAC‑‑CGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAA‑CACTGCTGGCGG‑TAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACC‑AGCAAGCG‑CGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCA  >  NC_000913/3293478‑3293949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`