Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,681 G→T 19.0% intergenic (+35/+30) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6810GT19.0% 32.0 / 2.5 35intergenic (+35/+30)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (14/9);  new base T (3/3);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATA  >  NC_000913/2151443‑2151912
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTg                                                                                                                                                                                                                                              <  1:561242/239‑1 (MQ=255)
     taatGGCGTAATCAGCAATGAACCTGGTATTGTTAAAAACGTAGAATATGGCTAGTATTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACGATTGATCCTTTATTGGTCGCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                                                                       <  2:947592/236‑1 (MQ=255)
     tATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttg                                                                                                                                                                                                                                       <  1:396693/239‑1 (MQ=255)
             tAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTTATTCTTTTTTTGTTACCTTTCCAAAAGAAGAAAACGACGAGCGAAGAGCAAAAATAGTTTCCTTATCAAACGTTATATTTTTTTTTTAAAATAGCGGTGTAATTTTTGttttt                                                                                                                                                                                                                               >  1:699365/1‑238 (MQ=255)
                                                        gcAGGGGGGGAAAAAAAAAAAAAAAGAATATTTAAGGTTGGATGTATACGCGGGGGGAAAAAATTTCGGGGGTTTATACCATTGATCTTTTTTTGGTCCCCTATCAAAAAGAAAACGCCGAAGAGAAAAGAGAAAAATTAGTGCGTTAATCAGCCTTTTTTTTTTTTTTTAAAACGGGGGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc                                                                                                                                                                                    <  2:468798/237‑1 (MQ=255)
                                                             aGTAGAAAAACAAAAAAGAATATAGTTAAGACTGGTGTTATAGGCGGGGGAATAAATTTCGCGGGTGTTATACATTTAATCCTTTATGGCCCTCCAACCAAAAAGAAAAAGCGGATGAGCTAAGAAAAAAATTAGTTCGATAGCCAGCGTTTATATTTTTTGTTTAACCGGGGGGTTGAATTGTTTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                              <  2:972473/238‑1 (MQ=255)
                                                                    aaTAAAAAAAAAAGATATTTCAGCCTGGATGTATCCGCGGGGGAAAAAAATTTACGGCGGTTATCCCATTGTTCTTTTATTGCTCACCTACCAAAAAGAAGAAGCCAACGAGAAAAGAAAAAAATATGTTCGTTAATCACCCTTTATATTTATTGTTTAACCTGGGGGTTAAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatc                                                                                                                                                                      <  1:443884/239‑1 (MQ=255)
                                                                                    tacctcatgcTGGAGTTACACGCGCTGGAATAAAATTTACGGGCTTTACACTTTGGACCCTTTATGGCACACCTAGCAAAAAAAAGGAGGCGACAGGTCAAGAAAAAAAAGTCTGCGCTACACACCCGTTTTATTTATTGTTTAGCCTGGGGGTAAATTTGTTTCCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGtt                                                                                                                                                      <  2:115863/230‑1 (MQ=255)
                                                                                                                                gTTATACCATTGATCCTTTATTGGTCCCCTACCAAAAAGAAAAAGCCGAGGAGCTAAGAGAAAAATTAGTCCGCTAACCAGCCGTTATATTTATTGTTTAAACGGGCGGTTGAATTGTTGTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat                                                                                                                                                                    <  1:211309/182‑1 (MQ=255)
                                                                                                                                       ctttGATCTTTTATTGGCCCCCTACCAAAAAGAAAAAGCCGAAGAGCTAAGAGAAAAATTAGTTCGTTAATCAGCTTTTATTTTTTTTTTTAAAACGGGGGTTTAATTTTTTTTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                   <  1:544188/237‑1 (MQ=255)
                                                                                                                                          tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGTATTTTTTTTTTCCACCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTATCCTCCTCCGTACCCTc                                                                                                                                                         >  2:211309/1‑182 (MQ=255)
                                                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCCACCGCCATGAGGAAATCAATCTGCCCCCAGCGCCAGTGCCAACCGTTTATCATTATCCGTTAGCCCGTGTAACGCCGGGCTGGACCAAAACTGCTTTTACAATTTCGGTTATAAATCGAgttgt                                                                                               >  1:430787/1‑239 (MQ=255)
                                                                                                                                                 ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATCCGTAAGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAAACGAGTTGTTCCTGc                                                                                         >  1:567766/1‑239 (MQ=255)
                                                                                                                                                  tATTGCTCCCCTACCAAAAAAAAAAAGCCGACGAGCTAAGAGCAAAATTATTCCGTAAATCAGCTGTTATATTTATTTTTAAAACTGGGGGTTAAATTGTTGTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg                                                                                        <  1:914921/239‑1 (MQ=255)
                                                                                                                                                       gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTCAACCCCCATGAGAAACTCACTCTGCCCCCAACGCCCGTGCCCACGGTTTTTCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACAAAAACATCTTTAACAATTTAGGGAATAAATCGAGTTGTTCCTGCGTtgct                                                                                   >  2:268980/1‑239 (MQ=255)
                                                                                                                                                        tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGTATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCCACGGTTTATCATCATCCGTACACTCGTTTTCCGCCCGGCCGGACAAAAACTGCTTTAACCAATCCGGGAATAAATCGAGTTGTTTCttggtttct                                                                                   >  2:977994/1‑230 (MQ=255)
                                                                                                                                                        tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGGTGTTTCTGCGTtgct                                                                                   >  1:897092/1‑238 (MQ=255)
                                                                                                                                                          aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCCCCCGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTTTTTCTGCggttcttc                                                                                 >  1:1091478/1‑231 (MQ=255)
                                                                                                                                                                                       aaGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTGTTCAACCCCCATTAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGGTGGGCAAAAACTGCTTTCACAATTTCGGCAATAAATCGAGTTTTTCCTGCGGTTCCGCCGCCAGTCCCATGGAAAACGGGGGaaagca                                                   >  2:749916/1‑237 (MQ=255)
                                                                                                                                                                                             aaaaTTTGTTCCATAAACAGCCCTTATTATTTTTTTTTAAACCGGGGGTTTAATTTTTTGTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca                                              <  1:295498/239‑1 (MQ=255)
                                                                                                                                                                                             aaaaTTGGTTCCTTAATAACCCTTTATTTTTTTTGTTAAACCTGGGGTTTAAATTTTTTCCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                             <  1:500990/239‑1 (MQ=255)
                                                                                                                                                                                                  tAGTTCGTTATACACCCTTTTTTTTTTTTATTAAACCTGGGGTTTAATTTTTTTTTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                        <  1:99038/239‑1 (MQ=255)
                                                                                                                                                                                                    tgtccatAATCAGCCGTTATTTTTATTGTTAAAACTGGGGGTTGAATTTTTGTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagga                                      <  1:307402/233‑1 (MQ=255)
                                                                                                                                                                                                         cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGATGTATTGTTTTTTTTCAACGCCATGAGAAAATAACTCTGCCCACAACCCCCATGCCCACGTTTTTTTATCATCCCGTACCTCTGTTACCGCCGGGGTGGACAAAAAATTCTTTTACAATTCATGGAATAAATAGCGGTGTTTCTGCGGTTCTTCCGCCAGTCCATTGTAAAACGGGGAAAAAAAAGGCACATGAGGGGAAtt                                 >  2:1231099/1‑239 (MQ=255)
                                                                                                                                                                                                            acaaGCCTTTTTATTTATTGTTAAACCGGGCGGTTGATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                              <  1:872061/236‑1 (MQ=255)
                                                                                                                                                                                                                 ccGTTATATTTATTGTTTAAACTGGCGGTTGGATTGATGTCTTCCGACGCCATCAGAAAATCACTCTTCCCCCAACGCCAGGGCCAACCTTTTATCATCATCCGAAAGCTCGTTTTTCCCCGGGCTGCACCAAAAATGCTTTAACAATTCCAGCAATAATTCATGTTGTTCCGGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGATAAACAAATGCACATGAGGAGAAGTATCCAGCa                         >  2:636701/1‑239 (MQ=255)
                                                                                                                                                                                                                            attGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCTTGAGAAAATCACTCTGACCACAGCGCCAGTGCCAACGTGTTTTCATCATCCGTTCGCTCGTTTAACGCCGCGCTGGACAAAAAATTCTTTTACAATTCAGGTAATAAATTGAGTTGTTTCTGCGTTGCTGCCGCCAATCCATTGGAAAAAGGGGAGAAAAAAGGGACAATAGGAGAATTATCCAGGATATTTAACGcc              >  1:1331392/1‑239 (MQ=255)
                                                                                                                                                                                                                             attattAAACCGGCGGTTTGACTGTTTGTCACCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGcc              <  1:1231099/235‑1 (MQ=255)
                                                                                                                                                                                                                              tGTTTAAACTGGCGGTTTAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg            >  1:753409/1‑239 (MQ=255)
                                                                                                                                                                                                                              tGTTTAAACTGGCGGTTTAATTGTTGTCTTCAAACCCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCGGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCCGTCCCTTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg            >  1:760198/1‑239 (MQ=255)
                                                                                                                                                                                                                              tGTTTAAACTGGCGGTTGAATTGTTGTTTTCAACCGCCATGAGAAAATCACTCTGCCCCCAACGCCAGTGCCCACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAATGCTTTTACAATTCAGGTAATAAACTGAGTTGTTCCTGCGGTGCTGCCGCCCGTCCATTTGAAAACGGGGGAAAGAAAAGCACAAGAGGAGGATTATCCAGCCTATTTAACGcccg            >  1:66511/1‑237 (MQ=255)
                                                                                                                                                                                                                              tGTTTAAACTGGCGGCTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACCCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTTCCGCCGGGCTGGACAAAAACTTCTTTAACAATTCCGGTAATAAATCGAGTTGTTCCTGCGGTTCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTTACGCCaa            >  1:884981/1‑238 (MQ=255)
                                                                                                                                                                                                                              tGTTAAAACGGCCGTTTAATTTTTTGCCTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg            <  1:1095048/239‑1 (MQ=255)
                                                                                                                                                                                                                                tttAACCGGGGGGTTAAATTGTTGTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                   <  1:1315240/150‑1 (MQ=255)
                                                                                                                                                                                                                                          gggTTGAAATTGTTTTTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa  <  1:1012115/237‑1 (MQ=255)
                                                                                                                                                                                                                                          cGGTTGAATTTTTTTCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATTATCATCCGTACGCCCGTTTTCCCCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGGAATAAATCGAGTTGTTTCTGc                                                                                         >  2:1315240/1‑150 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            
ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATA  >  NC_000913/2151443‑2151912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: