Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 766,690 T→A 16.0% L236H (CTC→CAC)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,6900TA16.0% 88.4 / 2.5 52L236H (CTC→CAC) mngAfused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base T (31/10);  new base A (3/5);  total (36/16)
Fisher's exact test for biased strand distribution p-value = 4.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGA  >  NC_000913/766453‑766922
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
cGGCGACGCCGCCTGATGATTTGCTCTCGGCGACGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACt                                                                                                                                                                                                                                              <  2:516280/238‑1 (MQ=255)
   cgacgaCGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCaa                                                                                                                                                                                                                                          <  2:1042807/238‑1 (MQ=255)
                         ctcGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCCCACACCTATCTGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGAGTTTAACGGCGGATACACACAATATAGCAACCCCCTGTAtttttt                                                                                                                                                                                                                   >  1:530517/1‑238 (MQ=255)
                                    aggACAGGGGAGGCCCCCACCCTTCTGCCTCTTTTCCCACCGCGCCAACTATCGTCGGCGCACCGGCCTGTCCGGGGGGTATTGCACCCCCTTATAGGGCTGCGAAATATATAAAAAAGGCCGGACGAAACCGCGGCGAAATTTTTTACGTAAAAAAACAAGGCGCAAACGGCATTGAAGGTCGTTAAACGGGGGATCACCAAAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGt                                                                                                                                                                                                         <  2:583480/236‑1 (MQ=255)
                                       aaaggggAGGCACCCACCCTCCTGCCTTTTTTCCCACCGCGCCAACTATGGTCGGCTTAACGGCGGGTCGGGGGGGTATTGCACACCCCTATATGGGTGCGAAATATGTGAAAAACGCCGGACGAAACCCCGGCGTAATTGTTTAGGTTAAAAAACAAGGCTCTAACGGCTTTGAGGGGCTTTAAACGGCGGAACAACACAATGGGGCAACCGCCTGTATTTTTGCGGCTGAAGTCGcc                                                                                                                                                                                                     <  2:1073399/235‑1 (MQ=255)
                                             gAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGGAGTCGCCATCAAg                                                                                                                                                                                               >  1:583480/1‑239 (MQ=255)
                                                      acccTTCTGCCTCTTTCACCAACGCCCCAATTTGCGTGTCCATACCGGCCTCGCGGGGGGGTTTGCCCCCCACATATGTGGGGGCGAAATATCAGAAAAAGGCCGGACGCAAACGGGGGATAAATTTTTAGGTTAAAAAACAAGGCGCAAACGGCATGGAGGGGTTTTTAACGGGGGTCCAACTAAATGGGGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAg                                                                                                                                                                                      <  2:1383485/237‑1 (MQ=255)
                                                           tCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGGAACCCCCTGTATTTTTGCGGCTGAAGAAGCCATCAAGGAGAGTGAGCGttt                                                                                                                                                                                >  1:506746/1‑239 (MQ=255)
                                                           tCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAAAAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAAACCCCTGTATTTTTTCGGCTGAAGTCGCCCTCCAGGAGAGGGAGCGttt                                                                                                                                                                                >  1:782982/1‑239 (MQ=255)
                                                              gCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGGTCCACCCACTAAGGCAACCCCCCGGATTTTTGTGGGTGTAGACCGCAACAAAGAGAGGGGGGGCTTTaa                                                                                                                                                                             >  2:1154028/1‑239 (MQ=255)
                                                                  tttttcCCACCGCTCCAATTTCCGTCTGGGTACCGGCCTGTCCGGTGGGTTTTGCTCACCCTTATGTGGCTGCAAAATATCTAAAAAAACCGGGACGAAACCTCGGAGAAAATTTTTACGTTAAAAAAAAGGGCGCTACCGGCGTTGAGGGGCGTTTAGCGGGGAATCAACTCAAAGGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGc                                                                                                                                                                         <  2:914158/238‑1 (MQ=255)
                                                                   tttttCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACAAATCAATAGTTCAACCCCCTGTATTTTTGGGGCTGAAGTAGCCATCAAGGCGAGTGAGCGTTTTGATTGc                                                                                                                                                                         >  1:384328/1‑238 (MQ=255)
                                                                             gcgcCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAATCAATAGTGCAACCGCCTGGATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTTCCGCGCt                                                                                                                                                             >  1:1511530/1‑239 (MQ=255)
                                                                                   aCTATGGTCTGCGAAGCCGCTTGTCGGGCGGGTATTCCGCACACCTAGATGGCGGCGGAATAGCTGAAAAAAGCCGGACCAAAAGTGGGCGTAAATGTTAACGTTAAAAAACAAGCGGCAAGCGGCATTGAAGGGGGTTAAAGGGGGGACCACCTAAAGAGTGCCGCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGt                                                                                                                                                       <  2:1395316/239‑1 (MQ=255)
                                                                                              cGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGGAACCCCCTGTATTTTTGTGGCCGCAGTCGGCCTCCAGGAGGGTGAGCGGTTTTATGGCATTCCCGCGCTTTCCAGGCCTGTTtccg                                                                                                                                           >  1:543662/1‑239 (MQ=255)
                                                                                              cGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAAATCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAAACGCCTGTATTTTTTGGGCTGAAGGCGCCATCAAGGGGGGTGAGCGGTTTTATGGGCTTCCCGGGCTTTCAGTGCCTGTTgccg                                                                                                                                           >  1:47887/1‑239 (MQ=255)
                                                                                                         gcccGGGGGATATTCCCCACCCCTATAGGGCTGCAGAATATCTGAAAAAAGCCGGACCAAACCGCGGGGTAAATTTTTACTTAAAAAAACAAGGCGCAAACGGCTTTGAGGGGTTTTAAACGGGGGATCAACTAAATAGTGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCg                                                                                                                                 <  1:1321666/236‑1 (MQ=255)
                                                                                                          tCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGGTAAAATCAATAAGGCAAACCCCTGTATTTTTTCGGCTGAAGGGGCCCCCAAGGAGAGGGAGCGGTTTTATGGCATTCCCCGCCCTTTAGTGCCCGTTGCCGGGGCGAGTCgg                                                                                                                                >  2:1055061/1‑237 (MQ=255)
                                                                                                                            cacCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAGCAAACCAAAAGAGCAACCGCCCGTAGTTTTGCGGCTGAAGCAGGCAACCAGGGGGGGGAGCGGTTTCATTGCATTCCCGCGCTTTCAGTGCCTGTTGCCGTGACGGTTCGCCATGCAGAAGCGTTGATcc                                                                                                             >  2:1194709/1‑239 (MQ=255)
                                                                                                                             acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGGGCCGATTTGCCATGccg                                                                                                                         >  1:1147672/1‑224 (MQ=255)
                                                                                                                             acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCca                                                                                                            >  1:1373290/1‑239 (MQ=255)
                                                                                                                             acCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAAAAGTGCCACCCCCTGTATTTTTTCGGCTGAAGGCGCCATCAAGGAGAGTGAGCGGTTTTATGTCATTCTCGCGCTTTCAGTGGCTGTTGGCACGCCGCTTCGCCCTGCCGAAGCGTTGTTctc                                                                                                            >  2:1026576/1‑237 (MQ=255)
                                                                                                                                      ggtgggaattatCGGAAAAAGGCCGGACAAAAACCCGGAGTAAATGTTTACGTAAAAAAACAAGGCGCTAAGGGCATTGAGGGGCGTTAAACGGCGGATAAACAAAATAGTGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAgcgc                                                                                                    <  1:441141/229‑1 (MQ=255)
                                                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGGAGTCGCCCTCCAGGAGAGTGAGCGTTTTAATTGCATTTCCGCGCTTTCAGTGCCCGTTGCCGAGGCGATTCGCCATGCCGGAGCGGTGTTCCAACCAGCCCtt                                                                                                  >  2:441141/1‑239 (MQ=255)
                                                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGGCGGCGCCATCCATGAGAGTGGGCGTTTTAATTGCATTCCCGCGCTTTCAGTTCCTGGTTCCCGTCCCGTTTGCCATGCCGCAGCGTTGTTCCAACAAGCGCtt                                                                                                  >  2:43329/1‑239 (MQ=255)
                                                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCtt                                                                                                  >  1:485056/1‑239 (MQ=255)
                                                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAAAAGTGCAACCGCCTGTATTTTTTTGGCTGAAGTCGCCATCGAGGAGAGTGAGCGGGTTAATGGCATTACCGCGCTTTCTGTGCCTGTCGCCGCGCCGGTTTTGCACGCAGGAGCGTTGATCCAAAAAgcgcct                                                                                                  >  2:957575/1‑237 (MQ=255)
                                                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAAAAGTGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCa                                                                                                                          >  1:1202592/1‑215 (MQ=255)
                                                                                                                                       cTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAATCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATAAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCCGAAGc                                                                                                                     >  1:225466/1‑220 (MQ=255)
                                                                                                                                        ggggaaaTATCGGAAAAAGGCCGGACGAAAACTCGGCGTAAATTTTTACTAAAAAAAAAAAGGCGCTACCGTCATGGAGGGGCGTTAACCGGCGAATAAACTAAATAGTAAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTa                                                                                                 <  1:1314106/234‑1 (MQ=255)
                                                                                                                                         gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAATCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGGCGCCATCAAGGGGAGGGAGCGGTTTTATGGCATTCCCGCGCTTTCCGTCCCTGTTGCCGGGGCGGTTTGCCACGCCGAACGGTTGATTCAACAAACGCTTa                                                                                                 >  2:1186174/1‑238 (MQ=255)
                                                                                                                                         gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAATCAATAGTACAACCGGCTGTATTTTTTTGGCTGTAGTCGCCATCAAGGGGAGTGAGCGGTTTATTGGCAGTCCCGCGCGTTCAGTGCCTGTTGCCGAGCCGGTTCGCCATGCAGAAGCGGTGGTCCAAGAAGCGCGTa                                                                                                 >  2:266912/1‑238 (MQ=255)
                                                                                                                                         gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAAAACAAACGGGCAAACGCCTGTATTTTTTCGGCTGAAGTAGCCACCTAAAGGCGGGAACGGTTTTAAAGGCTTCCCCTCCCTTCCAGTCGCGGTTCCCGGCCGCATCGCCATGTCCAAGCACTGCTCCAACAAGCGCTTa                                                                                                 >  2:1134261/1‑238 (MQ=255)
                                                                                                                                          gcgaaagacTGAAAAAAGGCGGACACAAACCCGGGAGAAATTGTTTAGGATAAAAACAGGGGCGTAAAGGGATTGGAGGGGTGTTAAGGGGGGAACCACTAAAATGGGGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTa                                                                                                 <  2:485056/230‑1 (MQ=255)
                                                                                                                                          gcaaaattcgagAAAAAGGCGGACCAAAACCCGGAGTTAATTTTTTCGTTTAAAAAAAAGGCCGCTAAGGGTTTTGAGGGTCGTTAAAAGGGGTACAACTTAAATTGTACAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTa                                                                                                 <  1:803907/227‑1 (MQ=255)
                                                                                                                                          cGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGGTTAACTCAAACGTGCAACCGCCTGTATTTTTGCGGAGGAAATCGCCCTCAAGGCACGGGAACGGTTTAAAGGCAGTCCCCCGCCTTTTATACCCGTTGTCCAGCCGCTTTGCCATGCAGAAGAGTGGATCctaccaaccgctac                                                                                                >  2:1336829/1‑228 (MQ=255)
                                                                                                                                             attagtgagAAAAGGCGGGACGAAACGGCGGCAAAATTGTTGAGGTAAAAAAAAAAGGCTAAAGCGGTATGGAGGGGCGTTAAGCGGCGGAAAAACAAAATAGCGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTc                                                                                            <  2:182843/231‑1 (MQ=255)
                                                                                                                                             aaTATCTGAAAAACGCGGGACACAAAGAGGGGTAAAGTGTTTACTTTGAAAAACGAGGCGCTAACGGCTTTGAGGGGCTTTTAGCGGCGAATCAACTCAATGGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTc                                                                                            <  1:290165/239‑1 (MQ=255)
                                                                                                                                                       aaaaGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACACAATAGTGCAACCGCCTGTATTTTTGCGGCCGAAGGCGCCCTCAAGGGGGGGGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGGGCCGATTCGCCATGCAGGAGCGTTGATCCAACCAACGCTTACCCTCAAACGTAGCg                                                                                  >  2:1356320/1‑239 (MQ=255)
                                                                                                                                                          aGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATAAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGAGAGCGTTTTAAAGGCATTCCCGCGCTTTCAGTGCCTGTTGGCGGGCCGATTAGCCATGCAGAAGCGTTGATCCAACAAACGCTTACCCTCAAGCGTAGCGATg                                                                               >  2:1450707/1‑239 (MQ=255)
                                                                                                                                                                                        tACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGGTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGGTTCGCCATGCAGAAACGGTGGTCCCACAAGCGCCTACCCCCCAGCGGAGCGGTGGGACGCGGACCGCACCGCAAGATACGCa                                                   >  2:606473/1‑239 (MQ=255)
                                                                                                                                                                                        tACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGGTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAg                                                              >  1:922045/1‑228 (MQ=255)
                                                                                                                                                                                        tACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCGGAAGGCGCCATCAAGGAGAGTGAGCGTTTTAAAGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATgag                                                                             >  1:13481/1‑213 (MQ=255)
                                                                                                                                                                                             taaaaaaCAAGGCCGCAACCGGATTGAAGGGGCTTTAACGGGGGGACAAACTAAATAGTGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAAc                                                 <  1:1055061/235‑1 (MQ=255)
                                                                                                                                                                                               aaaaaCAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGGCGCCATCCAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGGTCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGCACAGCAAGATAAGCAACCGGTg                                            >  1:1045199/1‑239 (MQ=255)
                                                                                                                                                                                                 aaaCACGGCTCTAGCGGGTTTGAAGGGCGTTAAAGGGTGTCACATCAAAAGAGAGCAGCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGGCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGaa                                          <  1:381756/239‑1 (MQ=255)
                                                                                                                                                                                                       ggCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCCATAGTGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTTCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGAc                                                                           >  1:838417/1‑200 (MQ=255)
                                                                                                                                                                                                       ggCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCCAGCGTAGCGATGAGAAGCGTACCGTAACGCAAGATACGCAACCGGGGAAAAgag                                     >  1:1288685/1‑236 (MQ=255)
                                                                                                                                                                                                                    cattgaggggttttAACGGGGGAAACAACGAAATAGCAAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAg                          <  2:782982/227‑1 (MQ=255)
                                                                                                                                                                                                                                        cggATCAACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGAAGTCGCCATCCAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGGTTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAACGTAGCGATGGGACGCGTACCGCACAAGAAGATACGCAACCGGGGAAAAGTGACAAAACGGAGCTGGAAAAGGACCTGGTGAga     >  2:309483/1‑238 (MQ=255)
                                                                                                                                                                                                                                        cggATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGc     >  1:1092822/1‑239 (MQ=255)
                                                                                                                                                                                                                                          gATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCgg   >  1:148991/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aTCAACTCAATAGTGCAACCGCCTGTATTTTTTCGGCTGAAGGCGCCATCAAGGAGAGGGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGGTTTGCCATGCAGAAGGGGTGTTTCAACCAGCGCTTTCCCTCAAACGGAGGGCGGGGACGCGTACCGGCCAACACGAAACGCCACCGGGGAAAAATGTCCAAAAGGGGCTGAAACAAGCACAGTTGAGCGGa  >  2:282214/1‑239 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
CGGCGACGACGCCTGATGAGTTGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGA  >  NC_000913/766453‑766922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: