| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,542,110 | A→G | 20.7% | S161P (TCC→CCC) | narZ ← | nitrate reductase 2 (NRZ), alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,542,110 | 0 | A | G | 20.7% | 138.5 / 4.9 | 76 | S161P (TCC→CCC) | narZ | nitrate reductase 2 (NRZ), alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (36/21); new base G (2/15); total (39/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGG > NC_000913/1541872‑1542339 | tCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGa < 2:1024390/239‑1 (MQ=255)tCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCCGGGTTAGTTTCT‑GCCCGTTGGa > 2:27296/1‑239 (MQ=255) gTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGGTCCT‑GCCCGTTTGAGC‑GGATCAA‑ccc > 1:454895/1‑239 (MQ=255) cTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGGTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑TCCCGTTGGAGC‑GGATAAA‑Cccgcccc > 1:963041/1‑237 (MQ=255) ccccccAGGCCGTCGGGGAGGCGGGCGGCAGGC‑CGCAATACCAGACGTAGAAACTTAAACAGGCGCCGCCAGGCCGCGACAAATAACGCGTGCGGGCGGCGTAAGAACCCGCCGACCTCGCGGGGATCGGAGAGAAACC‑GG‑CAACGCGCCCCGGGCCGTAGTTTTGGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCAAGTGGGAGC‑GGATAAA‑CCCGCCgcgc < 2:1305505/237‑1 (MQ=255) cccAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTCCCGGCGGCGTAAGAACCCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCa < 1:689983/239‑1 (MQ=255) acggTTATCGGCGAGGCGGGCGG‑AGGTCCGCATAACCAGTCGGAGAAACTAAAAAGGGGCCCGCCAAGCAGCGACAAATAACGCTTCCCGGCGGCTTAAGAACCCATCGACATCGCCGGGATCGGCGAAAACCC‑GG‑AACCGCGACCCGGCCCGTGGTTTTTGAGGGCCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGGGC‑GGAAAAA‑CCCGCCGCGCCCACg < 2:976320/237‑1 (MQ=255) aGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGGTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGc > 1:773113/1‑239 (MQ=255) gtacggggggAGGCGGGCGGGAGGC‑CGCAATCCCGGTCGTAGAAACTTAACCAGGTCCCCCCAGGAGGCGACAGATACCGCGTCCCGCGGGCGTAAAAAACCAGCGACAGCGCGGGGAGCGGCGAGAAACC‑GG‑CACCGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACg < 2:1713495/227‑1 (MQ=255) gcatgcggcggGGCGGGGGGAAGGT‑CGAAATCCCAGTCGGAGAACCTTAAACAGGTCCCGCCAGGCAGCGACAGAGACCGCGTTCCGGCGGCGTAAGAACCCATCGACATCGCCGGGATCGGCGAGAACCC‑GG‑CAACGCGATCGGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCG‑GCCAGTTGGGGC‑GGATAAA‑CCCGCCGCGCCCACGCa < 2:1690445/233‑1 (MQ=255) tcgccggcggGGGGGGCGGCAGGT‑CCAAATCCCAGGCGTAGAAACTTAAACGGGTGCCCCAAACCGGCGACAGAAACCGCGTCCGGGCGGCGAAAGAACCCAGCGACACCGCGGGGACCGGCGAGAACCC‑GG‑CACCGCGATCCGGCCGGAAGTTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTGGGGGC‑GGATAAC‑CCCGCCGCGCCCACGCAc < 2:957226/234‑1 (MQ=255) cATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAAAGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACtt > 1:910520/1‑239 (MQ=255) tCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGGTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACttgt > 1:1036023/1‑239 (MQ=255) cGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCCCttgtt > 1:976320/1‑239 (MQ=255) cGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGGTTTGATGGTCCAGACGTTAGCGGCGGCCATCCGCCGGTTTTAGTCCT‑TGCAGTTTGGGC‑GGGTAAA‑CCCCCCCCGCCCACCCCCctgtt > 2:1143216/1‑239 (MQ=255) ggcgggcgGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGGTCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAg > 1:641836/1‑238 (MQ=255) aGGT‑CGCAATACCAGTCGTAAAAACTAAAACAGGTCCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAACCCATCGACATCGCCGGGATCGGCGAGAACCC‑GG‑CAACGCGTTCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACt < 1:1332910/238‑1 (MQ=255) gT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAg < 2:728465/190‑1 (MQ=255) gT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAg > 1:728465/1‑190 (MQ=255) tACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCACTCCGCTGGTTTAGTTCCT‑GCCAGTTGGGGC‑GGATAAA‑CCCGCCGCGCCCACGCCCTTGCTTGTAGCCCAGGCAATTTTGCCGATc > 2:1103639/1‑239 (MQ=255) gTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCa > 1:1100658/1‑239 (MQ=255) gTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCa > 1:640418/1‑239 (MQ=255) gTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGATTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTCCTGATCGTTCATaa > 2:1715532/1‑239 (MQ=255) aGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATc > 1:1122435/1‑239 (MQ=255) caaccaaaaaCGGGCGCCGCCAGCCGGCGACAGAAAACGCGCTCCGGGGGCGTAAGAACCCCCCTCCACCGCGGGGACCGGCGAAAACCC‑GG‑CAACGCAATCGGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCGGGTTTAGTCCCT‑GCCAGTTGGCGG‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCg < 2:1243768/232‑1 (MQ=255) aaCTAAACCCGGTCCCGCCAGGCAGGAACAGATAACGCGTGCCGGCGGCGTAAGAACCCACCGACATCGCCGGGGCCGGCGAGAACCC‑GG‑CAACGCGATCCGGGCCGTAGTTTTTGATGGCCCAGCCGTTAGCGGCGGCAATCGGCTGGTTTAGTTCCT‑GCCATTTGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCg < 2:1600823/237‑1 (MQ=255) ttAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACtt > 1:161314/1‑195 (MQ=255) gtaaccgggTGCCGCCAAGCGGCGCCAAAAAACGCGTTCCGGGGGCTAAAGAAACCATCGACAGCGCGGGGATGGGCGAGAAACG‑GG‑CACCGCGTCCGGGGCGGTAGGTTTTGAGGGTCCAGCCGTGGGCGGCGGCAGTCGGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAC‑CCCGCGGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGcc < 2:910520/232‑1 (MQ=255) aaCAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTTCGGATCGGTCATAATCCATGCCCa > 2:121721/1‑238 (MQ=255) tGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGt > 2:555000/1‑239 (MQ=255) tGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGt > 1:780492/1‑239 (MQ=255) ggccccaaaGCAGCGAAAAAAACCGCTTCCCGGCGGGGAAAAAACCCACCACCACCGCGGGGACGGGCGAAAAACC‑GC‑CAACGCGACCCGGGCCGTAGTTTTTGAGGGCCAGGACGTTAGCGGCGGCAATCAGCTGTTTTAGTCCCT‑GCCAGTTGGGGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGc < 2:1122435/228‑1 (MQ=255) gccgccAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGGTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGGTCGTTCATAATCTATGCCCACGCCCGt > 2:1151829/1‑238 (MQ=255) gcagcGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCCGTACCGGATc > 2:1721946/1‑238 (MQ=255) cagcGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGCGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGGTTGTAGCCCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGCTc > 2:920960/1‑237 (MQ=255) gACAGATAACGCGTTCCGGCGGCGAAAAAAACCTGCGGCCTGGGCGGGACGGGGGAGAACCC‑GG‑GCACGGGACCGGGGCCGTAGGTTTGGATGGTCCAGCCGTTAGCGGCGGCAACCACCTGTTTTAGTTCCT‑GCCAGTTGGGGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCtgt < 2:1570700/238‑1 (MQ=255) aCAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTa > 1:1712518/1‑225 (MQ=255) cgcgTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCCCTTGTTTGTAGCTCAGGCCCTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTGGCTTGAg > 1:39534/1‑239 (MQ=255) gTTCCGGCGGCGTAAGGAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCCATCAGCAGGTTTAGTTCCT‑GACAGTTGGAAC‑GGATAAA‑CCCGCCGCCCCCACGCACTTGTTTGTAGCACCGGCACTTTTTCGGTTCGATCTTAATCGCTGCCCACGCCAATACCGGATCGCGGTGTGCCTTGAgg > 2:170036/1‑237 (MQ=255) ttCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCt > 2:586422/1‑239 (MQ=255) tCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGAAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGTTCGTTCATAATCGCTGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGTGGGCt > 2:994722/1‑238 (MQ=255) gcggcgTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTcgc > 1:1255057/1‑238 (MQ=255) ggaaagaaaaCCGTCGCCATCGCGGGGGGCGGGGAGAAACG‑GG‑AACCGCGTCCGGGGCCGTTGTTTTTGATGGCCCAGGCGTGAGCGGCGGAAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGGGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGcc < 2:1176277/231‑1 (MQ=255) tAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCCTAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAg > 2:858389/1‑239 (MQ=255) cATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCCGTACCGGGTCGCTGTGTTTCTTGAGGGCTTTGCGCCACAGTTCAATCa > 2:1447926/1‑239 (MQ=255) cATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACCCCAGTACCGGATCGCTGTGTTg > 1:615938/1‑210 (MQ=255) tccGACATCGCGGGAATGGGCGAGAAACC‑GG‑AAACGGGATCGGGGCCGTGGGTTTTGACGGTCCAGACGTAAGCGGCGGCAATCAGTTGGTTTAGTTCCG‑GCCAGTGGGAGG‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAg < 1:68100/237‑1 (MQ=255) cGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTc > 1:1725547/1‑239 (MQ=255) cGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGa > 1:982825/1‑190 (MQ=255) ggggaaaaCCGGG‑CAACGCGATCCGGGCGGTAGTTTTTGAGGGCCCGGAGTTTGGCGGCGGCAATCAGCTGTTTTAGTTCCT‑GCCAGTTGGGGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCgg < 1:273035/234‑1 (MQ=255) ccggaAACC‑GGCCACCGGGACCCGGGCGGTGGGTGTTGAGGGCCCAGCGGTTAGGGGCGGCACTGGGCTGGTTTAGTCCCT‑GCCAGTGGGGGC‑GAATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCgg < 2:456930/235‑1 (MQ=255) cacggtacccGGGCGGTGGGTTTTGAGGGCCCAGGCGTTAGCGGCGGCAATCGGCTGTTTTAGTTCCG‑GCCAGTTGGGGC‑GGAAAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGt < 2:780492/231‑1 (MQ=255) gcgATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCGGCTGGTTTATTTCCT‑GCCAGTTGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAg < 1:1103639/239‑1 (MQ=255) tCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTCCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAgcgc < 1:624034/238‑1 (MQ=255) gccGGGCTGTAGGTTTGGAGGGGCAAGCCGTGGGCGGCGGCACTCAGCGGGTTTATTTCCG‑GCTGGTGGGGGC‑GAAAAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCt < 2:1111248/238‑1 (MQ=255) ccgggtctgTAGTTTTGGATGGCCCAGACGTTAGCGGCGCCACTCAGCTTGTTTGGTTCCT‑CCTAGTTGGGGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCt < 2:459661/231‑1 (MQ=255) cccGGGCGTTAGGTTTTGATGGTCCGAACGTTAGCGGCGCCAACCGGCT‑GTTTACGTCCTGGCCAGTTGGAGC‑GAATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAgcgc < 1:843621/237‑1 (MQ=255) cggtggttttgGAGGGCCCAGACGTTAGCGGGGGCAATCGGTTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGAAAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTaaa < 1:27296/231‑1 (MQ=255) ccGTGGGTTTTGATGGTCCAGCCGTTAGCGGCGGCAATAAGCTGGTTTAGTTCCT‑GCCAGTTGGAGG‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTaaa < 1:940026/238‑1 (MQ=255) ccGTGGGTTTGGATGGTCAAGGCGTTAGCGGCGACAATCAGCTGGTTTAGTGCCT‑GCCAGTTGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTaaa < 1:1544546/238‑1 (MQ=255) ggggggTTTTGGTGTTCAAGCCGTTAGCGGGGGCAATGAGGTGGTTGAGTTCCG‑GCAGGTGGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGa < 1:1517856/235‑1 (MQ=255) aGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATAc < 1:555000/239‑1 (MQ=255) aGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGAGCTGTAAAGATa > 1:737393/1‑238 (MQ=255) aGGTTTTGAGGGCCAAGACGTTAGCGGCGGCAACCAGCTGGTTTAGTTCCT‑CCCAGTTGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATa < 1:1143216/238‑1 (MQ=255) gTTTTGATGGTCGACACGTAAGGGGGGAGAAGCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAgcg < 2:355764/225‑1 (MQ=255) tttgggtGGTCCAGACGTTAGGGGCGGCAATCAGTTGGTTTATTCCCT‑GCCAGTTGGAGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCa < 2:632340/232‑1 (MQ=255) tCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTATGGCTGTAAAGATACCAGGGGTAACtt > 2:1510957/1‑239 (MQ=255) tCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGAt > 1:355764/1‑225 (MQ=255) tCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACtt > 1:632340/1‑239 (MQ=255) aGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGCTCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTGTTTCAGGCGGTTAGCGCTGTAACGATACCTGGAGTAACTTGCGcccctgc > 2:822271/1‑234 (MQ=255) aGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGAACTTGTTTGTAGCTCAGGCACTTTTGCGGAACGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGATTCGCGCCACAGTTCACTCAGTCGGTTACGGATGAGCGGGTCTTTCAGGCGGTTAGCGCTGGAAAGAAACCAGGAGTAAATTGCGCGCcgcg > 2:1532861/1‑239 (MQ=255) aTCAGTTGGTTTGGTTCCG‑GCCAGTTGGGGC‑‑GATAAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCCCTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCcgcg < 1:1090988/239‑1 (MQ=255) aTCAGCTGGTTTGGTTCCG‑TCCAGTTGGGGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTGGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCcgcg < 2:1036023/239‑1 (MQ=255) cAGCTGGTTTATTCCCG‑GCGGTTTGGGGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCcgcg < 2:524778/237‑1 (MQ=255) tttAGTTCCT‑GCCAGGTGGAGC‑GAAAAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCAt < 1:1673381/238‑1 (MQ=255) ttAGTGCCG‑GCCAGTTGGGGC‑GGATAAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGa < 2:640418/239‑1 (MQ=255) tatgTTCCT‑G‑CTGTTGGAACAGGATCAC‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATGCCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGa < 2:592525/236‑1 (MQ=255) gCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAgg > 1:592525/1‑239 (MQ=255) | TCCGGTACGTCGGTTTGCTCGCCCCAGGTCATCGGCGAGGCGGGCGGCAGGT‑CGCAATACCAGTCGTAGAAACTTAAACAGGTGCCGCCAAGCAGCGACAGATAACGCGTTCCGGCGGCGTAAGAAACCATCGACATCGCCGGGATCGGCGAGAAACC‑GG‑CAACGCGATCCGGGCCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCT‑GCCAGTTGGAGC‑GGATAAA‑CCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCCAGTACCGGATCGCTGTGTTGCTTGAGGGCTTCGCGCCACAGTTCAATCAGTCGTTTACGAATGAGCGGGTATTTCAGGCGGTTAGCGCTGTAAAGATACCAGGAGTAACTTGCGCCACGCGGGCAGCCGCGAGGTTCATGATTGGGCAGG > NC_000913/1541872‑1542339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |