BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,760,389	A→C	14.5%	S287R (AGT→AGG)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,389	0	A	C	14.5%	276.1 / 7.7	128	S287R (AGT→AGG)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand): ref base A (67/38); new base C (3/19); total (70/58)
Fisher's exact test for biased strand distribution p-value = 2.45e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAATCATTC  >  NC_000913/3760157‑3760626

aaaTGCCCGCTGGGCGGGGGGTAGGAGGGCGGAGGGGCGGTAATTTTCCCCCCAGAGCTTCAGGTTGAAAAAAAGGACGCACGGGGGCTCCCAGCGCGGCGAGGTTCATTTAACCCGCGCGTAATGCAGGCTTCAGGGGGGGGCTTGGCGGGGGGGCAATCATCTC‑TTTTTTACCAACAATAATTCCGGCCTGCGG‑CCCGCCTAAAAC‑CTTGTCGCCGGAAAAACTCCCCAG‑ACTGCcg                                                                                                                                                                                                                                          <  2:39077/238‑1 (MQ=255)

 aaTGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TCTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGAcg                                                                                                                                                                                                                                          >  1:1324297/1‑238 (MQ=255)

               tgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCCAACAA‑CTTGGCGCCGGAGAAACTCACCAG‑ACTGACCCCCGCCGCCATCaa                                                                                                                                                                                                                            >  2:1491714/1‑238 (MQ=255)

                 cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACt                                                                                                                                                                                                                          >  1:1039300/1‑238 (MQ=255)

                    gTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑ACTGTCGCCCGAGAAACTCACCCG‑ACTGACGCCCGCCCCCAACAACTCCt                                                                                                                                                                                                                       >  2:828555/1‑238 (MQ=255)

                              gcAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTTCCAACAATAATTCCTGCCCGCGG‑CCCGCCTAACAA‑CTTGTCGCCCGAGAAAATCACCAG‑ACTGACGCCCGCCCCAATCAACTCCTGCGGCATTgg                                                                                                                                                                                                             >  1:832384/1‑238 (MQ=255)

                              gcAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCCCCATCAACTCCTGCGGCATTgg                                                                                                                                                                                                             >  1:584190/1‑238 (MQ=255)

                                  ggTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACCATAATTTCTGCCTGCGG‑CCCGCCTAACAA‑CTTTTCCCCGGAGAACACCACCAG‑ACTGACGCCCGCCCCAATCCACTCCTGCGGCCTTGGCCCtt                                                                                                                                                                                                        >  2:442631/1‑239 (MQ=255)

                                  ggTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCtt                                                                                                                                                                                                        >  1:451212/1‑239 (MQ=255)

                                      ggTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCg                                                                                                                                                                                                     >  1:1342721/1‑238 (MQ=255)

                                      ggTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCg                                                                                                                                                                                                     >  1:665680/1‑238 (MQ=255)

                                              ttCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CCTTTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCCCAATCCACTCCTGCGGCATTGGCCCTTTCGGCAAACCg                                                                                                                                                                                             >  2:1473424/1‑238 (MQ=255)

                                               tCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                           <  1:337458/239‑1 (MQ=255)

                                                cACCCAGAGCGTCGGGGTGAAAATAAAGACGCAACGGGGCTCCCGCGGCCGCGAGGTTAATTTTACCCGCGGGTAATGCACGCTCCAGGGGGTGTCTTGGCGGGCGGGCGATCTTCTC‑TTTTTTCCCAACAATAATTCCTGCCTGCGC‑CCCGCCTAACAA‑CTTGGCGCGGGAGAAACTCACCAG‑CCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTAc                                                                                                                                                                                          <  2:101769/239‑1 (MQ=255)

                                                         cTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCAGCTC‑TTTTTTACCAACAATAATTCATGCCTGCGG‑CCCGCCTAACAA‑CATGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCAATGGCTCTTTCCGCAAAACGCACTGGCTCAGa                                                                                                                                                                                 >  2:384191/1‑239 (MQ=255)

                                                            cAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCCGAGAAACTCAACCA‑ACTGACCCCCGCCGCAATCCACTCCTGCGGAATCGGCTCTTTCGGCAAACCGCACTGGCTAACATcc                                                                                                                                                                              >  2:1099535/1‑238 (MQ=255)

                                                                 tgtAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAAACCGACCa                                                                                                                                                                          >  1:767373/1‑238 (MQ=255)

                                                                 tgtAAAAAAAGACGCAACGTGGCTTCCAGCGGCGCGAGGGGCATTTTATCCGCGCGTAATGCCCGCTTCAGCGGGTGGTTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCGGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCCCCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCa                                                                                                                                                                          <  1:1644323/238‑1 (MQ=255)

                                                                   taaataaaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCg                                                                                                                                                                       >  1:1539888/1‑239 (MQ=255)

                                                                    aaataaaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGa                                                                                                                                                                      >  1:681365/1‑239 (MQ=255)

                                                                        aaaGGCGCAACGTGGCTTCCAGCGCCGGGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTT‑TTTTTTCCCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                                  <  1:383236/239‑1 (MQ=255)

                                                                        aaaGACGCACCGTGGCTTCCGGCGCCGGGGGTGTGTTTTTATCCCCTCGTAATGCCCGCTACAGCGGGTGGCTTTGCGGGCGGGAGATCATCTC‑TTTTTCACCAAAAATAATTCCGTCCGGCGC‑CCCGCCTAACAT‑CTTGTCGCCGGAAAACCCCCCCAG‑CCTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                                  <  2:451212/239‑1 (MQ=255)

                                                                         aaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGGACTGGCTAAGATCGACCAGCGAAccc                                                                                                                                                                  >  1:1313183/1‑237 (MQ=255)

                                                                           gACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTTCCAACAATAATTTCTGCCTGCGG‑CCCGCCTAACAA‑CTTTTCGCCGGAGAAACTCACCAG‑ACTGACGCCCCCCGCAAACAACTCCTGCGGCATTGGCTCTTTCGGCAAACCCTCCTGGCCAAAATCGACCCGCCCGCCaat                                                                                                                                                                >  2:674415/1‑236 (MQ=255)

                                                                             cGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTg                                                                                                                                                               >  1:144769/1‑237 (MQ=255)

                                                                             cGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑ATTGTCGCCGGAGAAAATCACCAG‑CCTGACGCCCCCCCCAATCAACTCCTGCGGCCTTGGCCCTTTCGGCAAAACGTACTGGCTAAGATCGACCCGCGAGCCACTGc                                                                                                                                                              >  2:1624852/1‑238 (MQ=255)

                                                                             cGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCCGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCCACCAGCGAGcccatg                                                                                                                                                               >  1:1345921/1‑233 (MQ=255)

                                                                              gCAACGTGGCTCCCAGCGCCGCGGGGGTCATTTTACCCGCGCGTAATGCACGCTTCAGCGGGTGGTTTTGCAGGGGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAC‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCt                                                                                                                                                            <  1:146623/239‑1 (MQ=255)

                                                                                   gTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGGGATCCTCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTACCAA‑CTTGTCGCCGGCGAAACTCACCAG‑CCTGAAGCCCGCCGCAATCAACTCCTGCCGCCTTTGCTCTTTCGGCCAACCGGACCGGCTAAGAACGGCCACCGAGCCACCGCCTAAAt                                                                                                                                                        >  2:616583/1‑238 (MQ=255)

                                                                                      gCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACCGACGCCCCCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGGACTGGCGAAGAACGACCAGCGAGCCCCTGTCTAAATCAg                                                                                                                                                     >  2:1534054/1‑238 (MQ=255)

                                                                                       cTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCCACAATAATTCCCTCCTGCGG‑CCCCCCTAACAA‑CTTTTCGCCGGAGAAACTCACCAG‑ACCGACCCCCGCCGCAATCCACTCCTCCGGCACTGGCTCCTTCGGCCACCCGTACTTGCTAAAATCGTCCCGCCGCCCACCGCCCAACTCAGt                                                                                                                                                    >  2:6576/1‑238 (MQ=255)

                                                                                            aGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCCTCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTTTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTCCCGGCTCAGATCCACCCGCCAGCCACTGCCTAAAACAGTCACTAc                                                                                                                                              >  2:160262/1‑239 (MQ=255)

                                                                                              cgccgcGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCCCCTAACAA‑CTTGTCGCCGGAGAAAATCACCAG‑ACTGACGCCCGCCCCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACgg                                                                                                                                            >  1:278158/1‑239 (MQ=255)

                                                                                                ccgcGGGGTTCTTTTTATCCGGGCGTAATGCACCCTTCAGGGGGTGGTTTTGCAGGCGGGCGATCATCTT‑TTTTTTACAAACAAAAATCCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCCCCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑a                                                                                                                                         <  1:910104/239‑1 (MQ=255)

                                                                                                      gggTCATTTTACCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCGGGCGGGCGATCATCTC‑TTTTTTACCAAAAATAATTCCTGCCGGCGG‑CCCGCCTAACAA‑CTTGTCGCGGGAGAAACTCCCCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATc                                                                                                                                    <  1:1693896/238‑1 (MQ=255)

                                                                                                                  ccGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCg                                                                                                                       >  1:274090/1‑239 (MQ=255)

                                                                                                                      gcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCCAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑GACATCCAGCTCTTTGCCGAgc                                                                                                                    >  1:1559774/1‑238 (MQ=255)

                                                                                                                       cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGCGAAAATCAACAG‑AATGGCGCCCGCCGCAATCAACTCCTTCGGCATTGGCTCTTTTTGCAAACCGTACCGGCTAAGACCGACCCGCGAGGCAATCCCTAAAACACGCACCACGGGCAACACCCA‑CTCATTGcccggcc                                                                                                                   >  2:130713/1‑233 (MQ=255)

                                                                                                                       cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                                  >  2:144625/1‑192 (MQ=255)

                                                                                                                        gTAATGCACGCTGCAGCGGGTGGCTTTGCAGGCGGGGGATCATCTT‑TTTTTTACCAACAATAATCCCGGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGGCGGAGAACCTCCCCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                           <  2:1358678/166‑1 (MQ=255)

                                                                                                                                  cTTCAGCGGGTGGCTTTGCAGGCGGGCGCTCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTTTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCCTTGGCTCTTTCGGCAAACCCTACTGGCCAAGa                                                                                                                                                                                 >  1:1358678/1‑166 (MQ=255)

                                                                                                                                   ttCAGGGGGTGGCTTGGCGGGCGGGCGATCATCTT‑TTTTTTACCAACAATAATCCCGGCCGGCGC‑CCCGCCAAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTa                                                                                                                                                                                    <  2:1718502/162‑1 (MQ=255)

                                                                                                                                   tcggggggggggCTTTGCGGGCGGCCTACCCCCTT‑TTTTTCACAACCAAAACTCCCCGCCGGGCC‑CCCCCCTAAAAA‑CTTTCCGCCGGAAAAACCCACCAG‑CCGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCg                                                                                                       <  2:440535/232‑1 (MQ=255)

                                                                                                                                    tCAGCGGGTGCTTTTCGGGGCGGGCGACTACCTC‑TTTTTTACAAACACAATTATCTCCCT‑CGC‑CCCCGTCAACAATTTTGCCGCCGGAAAACCCCCCCAT‑ACTGCCGCCCGCCGCAATCAGCTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCt                                                                                                     <  2:1223033/239‑1 (MQ=255)

                                                                                                                                     ccgCGGGTGGCTTTGCAGGCGGGCGATCTCCTCTTTTTTTCCCAACAATAATCCCTGCCGCCGC‑CCCGCCTACCAT‑CTTGTCCCCGGAAAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCt                                                                                                     <  1:224569/237‑1 (MQ=255)

                                                                                                                                        cGGGTGGCTTTGCAGGCGGGCGGTCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCCCTTTCGGCAAACCGTACTGGCCAAGATCTACCAGCGAGGCCCTGCCCCAATCAGTCACTCCGGG‑AAAATCCAGCTCTTTTCCGGACGCCACCAGTTCCGCGTcc                                                                                                  >  2:1049140/1‑237 (MQ=255)

                                                                                                                                        cGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGGGCGCCACCAGTTCCGCTTCa                                                                                                  >  1:1188396/1‑238 (MQ=255)

                                                                                                                                           gTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTACTTGCCCAGCGCCACCAGTTCCGCTTCATCTa                                                                                              >  2:581408/1‑239 (MQ=255)

                                                                                                                                           acaagttgttaGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTa                                                                                              >  2:14399/11‑239 (MQ=255)

                                                                                                                                            tGGCTTTGCAGGCGGCCGATCTTCTT‑TTTTTTACAAACAATAATCCCTGCCGGCGG‑CCCGCCTAACAA‑CTGGTCGCCGGAGAACCTCCCCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTa                                                                                              <  1:828555/238‑1 (MQ=255)

                                                                                                                                             ggCTTTGCAGGCGGGCGGTCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAAAAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCCGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTAt                                                                                             >  1:440535/1‑238 (MQ=255)

                                                                                                                                             ggCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCa                                                                                                                                                                          >  1:1718502/1‑162 (MQ=255)

                                                                                                                                             ggCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATc                                                                                            >  1:1512070/1‑239 (MQ=255)

                                                                                                                                             ggCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATc                                                                                            >  1:1420652/1‑239 (MQ=255)

                                                                                                                                                      gggggggtatcaccTG‑TTTTTCACAAAAAAAATTTCCCGCCTGGGG‑CCCGCCTAAAAT‑CTTCTCGCCGGAGAACCTCCCCAA‑CCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGt                                                                                    <  2:1534121/230‑1 (MQ=255)

                                                                                                                                                       ggggccgtacatcTT‑TTTTTTCCAAAAAAAAATCCCCCCCGGGGC‑CCCGCCAAACAC‑CTGGCCGCGGGAGAACCCCCCAAG‑ACGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg                                                                                   <  2:1188396/229‑1 (MQ=255)

                                                                                                                                                          ggcgATCCTCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACCCCTCCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGCCCAGCCAGCCACTTCCTAACTCAGTCACTACCGG‑AACATCCAGCTCTTTGCCGAGCGCCACCCGTTCCGCTTCATCTTACGCTTTTGTGGAc                                                                                >  2:1107120/1‑238 (MQ=255)

                                                                                                                                                          ggcgATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                           >  2:608014/1‑132 (MQ=255)

                                                                                                                                                          ggcgATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                           <  1:608014/132‑1 (MQ=255)

                                                                                                                                                             gctcaccTC‑TTTTCCACCAACAAAATTCCCCGCCCGCGC‑CCCGCCAAACAT‑CTTGTCGGCGGAGAACCTCACCAG‑ACTCACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                                            <  2:681365/237‑1 (MQ=255)

                                                                                                                                                             gaccatcTC‑TTTTTTACCAACAATACTCCCTGCCGGCGC‑CCCGCCTAACAC‑CTTGCCCCCGGAAAAACTCACCAG‑CCGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                                            <  1:790791/236‑1 (MQ=255)

                                                                                                                                                             gATCATCTT‑TTTTTTACCAAAAATAATCCCTGCCTGCGG‑CCCGCCTAACAA‑CTGGTCGCGGGAAAACCTCCCCAG‑CCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                                            <  1:1491714/239‑1 (MQ=255)

                                                                                                                                                               tcatcTC‑TTTTTTACCAACAATAATTCCTCCCTGCGG‑CCCGCATAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGa                                                                          <  1:1613695/239‑1 (MQ=255)

                                                                                                                                                                         ttttACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAg                                                                 >  2:1441709/1‑239 (MQ=255)

                                                                                                                                                                          ttcacaaacaaTAATCCCTGCCGGCGC‑CCCGCCTAAAAC‑CTTGCCCCCGGAAAAACCCCCCAG‑ACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGt                                                                <  2:278158/233‑1 (MQ=255)

                                                                                                                                                                          ttcaCCAACAATAATCCCCGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACCCCCCAA‑GCAGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGt                                                                <  1:195421/236‑1 (MQ=255)

                                                                                                                                                                          ttaaCCAACAATAATCCCCGCCGGCGG‑CCCGCCTAACAA‑CTGGCCGCGGGAGAAACTCACCAG‑CCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGt                                                                <  1:869867/236‑1 (MQ=255)

                                                                                                                                                                            tACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                               >  1:250434/1‑238 (MQ=255)

                                                                                                                                                                            tACCAACAATAATCCCTGCCGGCGG‑CCCGCATAACAA‑CTGGCCGCCGGAAAAACTCCCCAG‑CCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                              <  1:930158/239‑1 (MQ=255)

                                                                                                                                                                            tACCAACAAAAATTCCTGCCGGCGG‑CCCCCCTAACAA‑CTGTCCGCGGGAAAACCCCCCCAG‑CCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                              <  1:930173/239‑1 (MQ=255)

                                                                                                                                                                            cccaaaaaataatCCCCGCCTGCGC‑CCCCCCTAACAT‑CTGGCCGCGGGAAAACCACCCCAG‑ACGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                              <  2:1485890/232‑1 (MQ=255)

                                                                                                                                                                                      atttCCTGCCGTGGC‑CCCGCACAACAA‑CTTGTCGCCAGAGACTCTCCCCAG‑CCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                                    <  1:593726/237‑1 (MQ=255)

                                                                                                                                                                                      actccctgccggCGG‑CCCGCATAACAC‑CTGGCCGCGGGAAAAACCCCCCAG‑ACGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                                    <  2:1554890/235‑1 (MQ=255)

                                                                                                                                                                                      actccctgcc‑gcGGCCCCCCCTAAGAC‑CTGTTCGCCGGAGAACCTCCCCAG‑ACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                                    <  1:249477/235‑1 (MQ=255)

                                                                                                                                                                                      aatcccgccctgcGG‑CCCGCCTAACAA‑CTGGCCGCCGGAGAAACTCACCAG‑CCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCTACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                                    <  1:1049140/231‑1 (MQ=255)

                                                                                                                                                                                      aatCCCTGCCTGCGG‑CCCGCCTAACAC‑CTGGTCGCCGGAGAAACCCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                                    <  1:355184/239‑1 (MQ=255)

                                                                                                                                                                                      aatCCCTGCCTGCGG‑CCCCCCTAACAA‑CTTGTCGCCGGAGAAACTCCCCAG‑CCTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                                    <  1:581408/239‑1 (MQ=255)

                                                                                                                                                                                           ctcccgggcg‑CCCCCCTAAAAC‑CTTGTCGCCGGAGAAACTCCCCAG‑CTGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtc                                               <  1:1385890/230‑1 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGCAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGGACTgg                                                                                                                                                                                       >  1:94077/1‑102 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                              >  1:799228/1‑238 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                              >  1:1261924/1‑238 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                              >  2:1169991/1‑238 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                              >  1:1482497/1‑238 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                              >  2:1022698/1‑238 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                              >  1:1542166/1‑238 (MQ=255)

                                                                                                                                                                                             gcctgcGG‑CCCGCCTAACAA‑CTTGTCCCCGGAGAAACCCCCCAG‑ACGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtcat                                             <  2:173677/239‑1 (MQ=255)

                                                                                                                                                                                                  cGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑CCTGACGCCCGCCGCACTCAACTCCTGCGGAATTGGCTCTTTCTGCAAACCGTACTGTCTAAGACCGACCATCGAGCCACTGCCTACATCAGTCACTACGGG‑AACATCCAGCTCTTTCCCGCGCGCAACCAGTTCCGCTTCATCTATCCCTTTAGAGAACCCCTGAATGCTGTAGTTACTGGTATGTACTCTCATCAACa                                        >  2:990297/1‑239 (MQ=255)

                                                                                                                                                                                                  cGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                                        >  1:536168/1‑239 (MQ=255)

                                                                                                                                                                                                  cGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                                        >  1:446555/1‑239 (MQ=255)

                                                                                                                                                                                                          ccTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGt                                 >  1:237010/1‑239 (MQ=255)

                                                                                                                                                                                                           cTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTa                                >  2:1056921/1‑239 (MQ=255)

                                                                                                                                                                                                                    ttgccccgggAGAACCCCACCAA‑CCTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcatt                        <  2:938834/231‑1 (MQ=255)

                                                                                                                                                                                                                    ttGGCGCGGGAGAACCCCCCCAG‑ACGCACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcatt                        <  2:1362537/239‑1 (MQ=255)

                                                                                                                                                                                                                     tGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc                       >  1:1362537/1‑239 (MQ=255)

                                                                                                                                                                                                                      gTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc                       >  1:1164300/1‑238 (MQ=255)

                                                                                                                                                                                                                         gCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCAcc                    >  1:743583/1‑238 (MQ=255)

                                                                                                                                                                                                                            ggAGAAACTCCCCAC‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCt                <  1:130713/239‑1 (MQ=255)

                                                                                                                                                                                                                               gaAACTCACCAG‑ACTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGa              <  1:1452244/238‑1 (MQ=255)

                                                                                                                                                                                                                               acaaCTCCCCAGAACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGcc                 <  2:144769/234‑1 (MQ=255)

                                                                                                                                                                                                                                            aCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAATCATTc  >  2:586093/1‑238 (MQ=255)

GAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTC‑TTTTTTACCAACAATAATTCCTGCCTGCGG‑CCCGCCTAACAA‑CTTGTCGCCGGAGAAACTCACCAG‑ACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGG‑AACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACGATAATCATTC  >  NC_000913/3760157‑3760626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`