Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 989,943 T→G 21.5% S138S (TCA→TCC) ‡ pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913989,9430TG21.5% 127.5 / 8.0 85S138S (TCA→TCC) ‡pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (21/41);  new base G (15/7);  total (36/49)
Fisher's exact test for biased strand distribution p-value = 6.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCG  >  NC_000913/989707‑990178
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                                                             <  2:348975/238‑1 (MQ=255)
gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                                                             <  2:666187/238‑1 (MQ=255)
  tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                                                           <  2:116440/238‑1 (MQ=255)
       tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                                                                     <  1:900339/239‑1 (MQ=255)
         ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                                                                   <  1:251466/239‑1 (MQ=255)
           cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAAAGCCGAGAAAGCGGATAAATTGCTTTCCAGCGGGTCGAGGGGTTGCGCAAAGTCGGCCTGCGGGGggcgagagcggt                                                                                                                                                                                                                                 >  1:890454/1‑237 (MQ=255)
            gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  2:444842/239‑1 (MQ=255)
            gTGAGGGAAAGCCGACGCGCCAGATCGTAGTGGCTGGTGCCCACAAACCGGGATTCCTGTTGCAGACGCTTAACGATGGCTTCTTGTACCTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  2:420003/239‑1 (MQ=255)
             tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  2:789900/238‑1 (MQ=255)
                     aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCCGGAAGGAGAATAATTTTGTTTTCCACGTGGCGGGGGGTTGTGCAAAGTCGGCCTGGGGGGAGCGTAAGCGGTGGACCCtttt                                                                                                                                                                                                                      >  1:1118632/1‑237 (MQ=255)
                          aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGCGAAGTCGAACAATTTGCTTTCCAGCGGGTGGAGGGCGTGCGCAACGTCCGCCTGCGGGGGGGGCTAGCCGGGTGCCATTTCCCttg                                                                                                                                                                                                                 >  2:1025507/1‑237 (MQ=255)
                           cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGa                                                                                                                                                                                                                 <  1:91303/238‑1 (MQ=255)
                             cgcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGGGAAGTCGGCTCATTTTCTTTTCAGCGTGTCGGGGGGTTGCGGAAACGCGGGCTGCCGGGGGCGGCAGCGGTGTACCCTTTCTCTGATa                                                                                                                                                                                                               >  2:1126734/1‑238 (MQ=255)
                                  acgcGTAGTTGCGGGGCCCCACAAACCAGGATACCGTTGGCAGCCGTTTAACGATGGTTTTTTGTACTTCGCGAGAAAAACGGCGCCGGGGGCCAAAACCCATCAGATGGAAGCGCGACATACCAAGCCCGGCGGTTACCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGGCGAGGGCTTGCGCAACTCCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGcc                                                                                                                                                                                                         <  2:1036234/236‑1 (MQ=255)
                                        aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGACGGGTGCCAAAACCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCaa                                                                                                                                                                                                    <  1:952787/238‑1 (MQ=255)
                                        aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGGCTAATTTGCTTTCCAGCGTGTCGAGGGGTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCATTTCACTGATAAACGCCCGCaaa                                                                                                                                                                                                   >  1:1005922/1‑239 (MQ=255)
                                               ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGGTAGCGATGTACCCTTTCACTGATAACCGCCCGCAAAGGAACt                                                                                                                                                                                             >  1:477396/1‑238 (MQ=255)
                                               ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTAACATTTCACTGATAAACCCCCGCCAAGGaaat                                                                                                                                                                                             >  2:1083384/1‑236 (MQ=255)
                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCCGCGTGTCGGGGGCGTGCGCAACGGCGGCCTGCGGTGGGCGATAGCGATGTACCATTTCACTGATAACCGCCCGCAAAGGAACATCCCAGAGGGTGAATTCACGc                                                                                                                                                                        >  1:1147389/1‑238 (MQ=255)
                                                                      gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAAAGCCGCGAAGTCGACTAAATTGCTTTTCAGCGGGGCGGGGGCCTGCGCAAAGTCGGCCTTCGGTGTGCGGTAGCGGAGGACCAGTTTAATGATAAACCCCCGGCAAGGAACTTTCCCGGGGATGGATTTACGccc                                                                                                                                                                      >  2:563338/1‑237 (MQ=255)
                                                                        tgaaGACGGTTACGGAGGGTTTTTTGTACTGCGCGAAAAAACGGGCGAGGGGTGCAAAAATCCATCAGAGGGAAGCGCGACATACCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTGGAGGGCTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                                                                    <  2:696236/235‑1 (MQ=255)
                                                                         gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCGGGGGGTTGCGCAACGACCGCCTGCCGGGGGCGGTAGCGCTGGAACATTTCACTCATAACCGCCCGCAAAGGCACTTCCCCGCGGGTGACCTctcccccccg                                                                                                                                                                   >  2:373855/1‑229 (MQ=255)
                                                                            gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCGGAAGTCGACTAATTTGCTTTCCAGCGGGTCGGGGGGTTGCGCCAAGGCGGCCCGCGGGGAGCGCAAGCGATGTAACCTTTCTCTGATAAACCCCAGCAAAGGGAATTCCCCGGGGATGACTTCACGCCCCGGGcc                                                                                                                                                                >  2:383357/1‑238 (MQ=255)
                                                                            gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGACTAATTTTCTTTCCAGCGCGTCGGGGGCTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCACGGGcc                                                                                                                                                                >  1:590856/1‑238 (MQ=255)
                                                                                             tCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGCGAAATCGCCTAATTTTCTTTCCAGCGTGTCGGGGGCTTGCGCCACCGCGGCCTGCGGGGAGCGGTAGCGGTGTAACATTTCACTGGTAACCCCCCCCCAAAGAACTTTCCCGAGGGTGAATTTTAGCCCCGGGCCCCTTTAAAGAatttaca                                                                                                                                               >  2:378376/1‑233 (MQ=255)
                                                                                               tttgaCTGCGCGAAAAAAGCGGCTACGGGTCCCAATATCCAACAGAGGGAAGCGCGACAAATAGAGACCGGCGGTTAACGCCGAGAGGTCGACTAATTTGCTTCCCAGCGGGTCGAGGGTTTGCGCAATTTCGGCCTGCGGTGAGCGATGAAGTTGTACAATTTCCCTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt                                                                                                                                            <  2:800021/235‑1 (MQ=255)
                                                                                                     ttCGCGAGAAAAACGGCGAGGGGTGCCAAAATCCATCAGAGGGAAGTGGAAATACCAAAGACCCGGGGTTAGCGCAGGAAGGTCCATTATTTTTTTTCCACGGTTCTCGAGGGCTTGCGAAACTTCGGCTTGGGGGGGGGGATGGGAATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                      <  2:418862/239‑1 (MQ=255)
                                                                                                     tgcgcgAGAAAACCGGGGGCGGGGGAAAAAACCAAACGAATGGAAGCGGGACATATCAGGCCGGGCGGTTACCGCCGAGAAGCCGACTAATTTGCTTTCCAGCGTGTGGAGGGTTTGCGCAACGTCGGCCGGGGGTGAGCGAGAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                      <  2:259729/237‑1 (MQ=255)
                                                                                                       ggcgAGAAAACGGCGGCGGGGGGCCAAAAGCCATCAGACGAAAGCGCGACATTTCAAGCCCGGCGGTAAACGCCGAGAAGCCGCTTATTTTGCTTCCAAGCGTGTGGAGGCCTTGCGCAACGTCGGCGTGCGGTGAGCGATAGGGATGTACCTTTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa                                                                                                                                    <  2:606028/238‑1 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGCGAAGTCGACTAATTTTCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTACCATTTCACTGATAACCCCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGGCGCCTAAAAGGAAATCCAACTTGCCAttt                                                                                                                                    >  1:1103764/1‑238 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGACTAATTTTCTTTTCAGCCGGTCGAGGGCTTGCGCAACCACGGCCTGCGGGGAGCGATAGCGGTGTACCATTTTACTTATAAACCCCCGCAAAAGAAATTCCCACAGGAAGACTTCACCCCACCGGCCGACTAAAAGAATATCCAACCTGTCATTa                                                                                                                                    >  2:737493/1‑239 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTTCAGCGTGTCCAGGGCTTGGGAAACATCGGCCTGCGGTGAGAGCTAAGGCTGTTCCATTTCACTTAATAACGCCACACAAGAAACATCCCCGCGGGTGACCTCAACCCCCGCGGCCCTTAAACGGAAATCCaacctcacctta                                                                                                                                    >  2:742550/1‑228 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAACACCGAAAAGTAGACCAATTTGCTTTTCAACGTGGTGAAGGCTTTCGCAAAGTCGGGCTGCGGTTCGGGGTAGCGCGGAACCAGCTCATCGATTACCGCCACCAAACGAAGTTCCCCGAGGATGACGTCACTCCACGGCACCCTTAAAAGAATATCCAGCTTGGCAtgt                                                                                                                                    >  2:699412/1‑237 (MQ=255)
                                                                                                       cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGCTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCCTGTCGAGGGGTTTCGCAAAGTCGGCCTGCGGGGGGCGCTAGCGATGTAACATTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGATGACTTTACCCCCCGGGCCGCCTAAAAGAATATCCAGCTTGGCATTa                                                                                                                                    >  2:125872/1‑239 (MQ=255)
                                                                                                         cgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCCGCGGTGAGCGATAGCGATGTACCATTTCACTGTTAACCGCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACCCCACGGGCCGCCTAAAAGAAAAATCAGCTTTCCATTATc                                                                                                                                  >  1:105888/1‑239 (MQ=255)
                                                                                                             aaaaaCGGCGGGGGGTCGCAAACCCCACAAGATGGAACGGCGACATACAAAAGCGGGCGGTTAACGCAGAGAAGTCGCTTATTTGCTTTTCCAGCGTGTGGAGGATTTGGCCAAGGTCGGCCTGGGGTGGGCGAAAGGGATGTCCCATTTCATGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                                                              <  2:136731/239‑1 (MQ=255)
                                                                                                                                           agagGGAAGGGGGACATAACAAGACGGGGGGTAACCGCGGAGAAGCCGACTATTTTGCTTCCAGGGGGGGCGGGGGCTGGCGAAACGTCGGCCGGCGGGGGGCGATGGCGATGTCCCATTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAg                                                                                                   <  2:590856/237‑1 (MQ=255)
                                                                                                                                               ggAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGACTAATTTGCTTTCCAGCGTGTCGAGGGCGTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAAACACCCGCAAAAGAACTTCCCAAAGGGAGACTTCACGCCCCCGGGCGCCTAAAAGAAAATCCACCTCGCCATTATCGTTTGTCACGGTGACTTGTTTCGGGTTTAAGCGGaa                                                                                              >  2:79422/1‑238 (MQ=255)
                                                                                                                                                 aaGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGTCGAGGGCTTGCGCAAAGTCGGCCCGCGGGGGGCGAAAGCGATGTACCATTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCTTCAACGAAAATCCCACTTTCCATTTTCGTTTGACACGGTGACTTGGTCCGGGGTAAAGCGGaaacc                                                                                           >  2:48225/1‑236 (MQ=255)
                                                                                                                                                      cgACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTTCTTTTCCGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTTACTGATAACCGCCCGCAAAAGAAATTCCCAGAGGGTGACTTCCCGCCACGGGCCCCTTAAAAGAATATCCAGCTTTCCATTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGAACTCGCGTa                                                                                      >  1:822188/1‑239 (MQ=255)
                                                                                                                                                       gacaagtCAAGACCGGGGGATAACGCCGAGAAGTCGACGATTTTGCTTTCCGGGGGGTCGAGGGCTTGCGCAACGCCGGCCTGCGGTGGGTGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:671591/233‑1 (MQ=255)
                                                                                                                                                       gACATTACAAGCCGGGGGGTTATCGCCGAGAGGTCGGCTAATTGTCTTGCCGTCTGTTCGGGGGCTGGCAAAACGCCGCCGGGGGGGGGGCGATAGCGTGGTACCATTCCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:1118632/239‑1 (MQ=255)
                                                                                                                                                       gACATATCAGGCCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTGGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCTTTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:48225/239‑1 (MQ=255)
                                                                                                                                                        atatatAAAGACCGGCGCTCACGGGCGAGAAGTCGCCTAATTTGCTCTCCAGCGTTTCGAGGGCTCGCGCAAGGTGGGCCGGCGGTGAGCCAAGGCGATGTACTATTTCACTGAGCGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTGCGCGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:608915/236‑1 (MQ=255)
                                                                                                                                                         acagtaaaggcccGGGGTTTCACGCCGAGAAGCCACTTATTTTTCTTCCCTGCGTGGCGTGGGTTTGCGAAACGGCGGCCTGGGGTGAGTGAATGCGTGTTACCATTTAACTGAGACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:128173/227‑1 (MQ=255)
                                                                                                                                                         aaatacgaaGACGGGCGGTTAGCGCCGAGAAGTCGGCTATTTTGCTTCCCGGCGTGGCGAGGTCTTGCGAAACGCCGGCCTGGGGTGAGCGATAGCGAGTTACCATTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:1147389/231‑1 (MQ=255)
                                                                                                                                                          aatagcagacccGGAGTTTAACGCCGAAAAGTCGTCTATTTTGCTTTCCGGCGTGTCGAGGGCTGGCGAAGCGCCGGCCGGGGGTGAGGGATAGGGATGTACCATTCCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                    <  2:105888/228‑1 (MQ=255)
                                                                                                                                                             tCAAGACCGGCGTTTAACGCCGAGAAGTCGACTAATTTGCTTTCCGGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGtt                                                                               <  1:506583/239‑1 (MQ=255)
                                                                                                                                                              cAAGGCCGGCGGTAAACGCCGAGAAGCCGTCTAATTTGTTTCCCAGGGTGTCGAGGGCTTGCGCAACCTCGGCGGGGGGGGGGCGAAAGCGATGGCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                                                              <  2:101964/239‑1 (MQ=255)
                                                                                                                                                                     cggcggTTAACGCCGAGAAGTCGACTAATTTTCTTTCCAGAGTGGCGAGGGCTTGCGCAACGTCGGGCCGCGGGGAGCGCTAGCGATGGACCATTTCACTGATAACCGCCCGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACCGGCCGCTTAAACGAATATCCAGCTTGCCCTTATCGTTGGCCACGGGGACCTGTTCCGGGGTAAAACGGGACTCGCGGAAACAGTTAAGATaa                                                                        >  2:770658/1‑238 (MQ=255)
                                                                                                                                                                      ggcggTTCACGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGTCGGGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCTCTGATAAACCCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGGCGCTTAAACGAATATCCAGCTTGCCATTATTGTTGGACCCGGTGACTTTTTCCGCGTTAAAGCGGCCCACGCTGAACCAGCTAAAATat                                                                        >  2:865326/1‑237 (MQ=255)
                                                                                                                                                                       ggggTTACCGCCGAGAAGCCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                       <  1:1083384/236‑1 (MQ=255)
                                                                                                                                                                       gcggTTAACCCCGAGAAATCGACTAATTTTCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGGATCCTTTCACTGATAACCGCCAGCCAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCCTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                                                                      >  2:162345/1‑239 (MQ=255)
                                                                                                                                                                       gcggTGACCGGCGAGAAGTGGATAATTTTGCTTTCCGGGGTGTCGAGGGTTGGCGAACCGCCGGCCTGGGGAGGGCGATAGCGATGACCCATTTCACGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                       <  2:508199/238‑1 (MQ=255)
                                                                                                                                                                            tagcgacGAGAAGTCTACTAATTTGCTTTCCAGCGGTTCGAGGTCTTACGCACCGTCGGCCGGCGGTGAGGGATAGCGATGTACCATTCCACTATTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:956524/233‑1 (MQ=255)
                                                                                                                                                                            taaggcgaagagGTCGACTAAGTTGCTTTCCTGCGTGTCGGGGGCTGGCGCAGCGGCTGCCGGGGGTGAGCAATAGGGATGACCATTTTCACTAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  2:373052/231‑1 (MQ=255)
                                                                                                                                                                            tAACGCCGAGAAGTCGTCTATTTTGCTTTCCGGCGTGTCGGGGGTTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCTTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:383357/239‑1 (MQ=255)
                                                                                                                                                                            tAACGCCGAGAAGTCGACTAATTTGCTTTCCGGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:770658/239‑1 (MQ=255)
                                                                                                                                                                            tAACGCCAAGAAGCCGACTAATTTGCTTTCCGGCGTGTCGAGGCCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:860154/239‑1 (MQ=255)
                                                                                                                                                                                 ccGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                                                            >  2:261482/1‑239 (MQ=255)
                                                                                                                                                                                         ccGACTATTTTGCTTCCAAGCGTGTGGAGGGCTGCCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata                                                                                                                                                   <  2:1051242/143‑1 (MQ=255)
                                                                                                                                                                                          cGACTAATTTGCTTTCCACGGGTCGACGTGCGGGGCAAACGTCGGCCGGCGGTGCGGCATAGCGTTGGCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca                                                     <  2:553477/237‑1 (MQ=255)
                                                                                                                                                                                                aTTTGCTTCCCGGGGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGGGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                             <  1:756915/239‑1 (MQ=255)
                                                                                                                                                                                                aTTTGCTTCCCAGCGTGTCGGGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTCCCATTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                             <  1:31666/239‑1 (MQ=255)
                                                                                                                                                                                                    tGCTTTCCGGCGTGTGGGGGGCTTGCGCAACGTCGGCCTGGGGTGGCAGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                          <  1:368407/239‑1 (MQ=255)
                                                                                                                                                                                                    tGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc                                                                                                                                         >  1:1051242/1‑144 (MQ=255)
                                                                                                                                                                                                    tGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGACCTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg                                           >  2:458893/1‑238 (MQ=255)
                                                                                                                                                                                                      cTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                        >  2:168276/1‑95 (MQ=255)
                                                                                                                                                                                                      cTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCGGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                        <  1:168276/95‑1 (MQ=255)
                                                                                                                                                                                                        ttcccAGCGTGTGGGGGGCTTGCGCAACGCCGGCCGGCGGTGAGCGATAGCGATGTACCATTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                       <  1:1025507/236‑1 (MQ=255)
                                                                                                                                                                                                         tttcaggCGTGTCGAAGGCTGGCGCACGGTCGGCCTGCGGGGAGCGATGGGGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                       <  1:499859/233‑1 (MQ=255)
                                                                                                                                                                                                          tCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                     >  1:635268/1‑239 (MQ=255)
                                                                                                                                                                                                          tCCAGCGTGTCGAGGGCTTGCCCAACGTGGGCTTGCGGTGGGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                     <  1:125872/239‑1 (MQ=255)
                                                                                                                                                                                                                               gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                    >  1:736279/1‑173 (MQ=255)
                                                                                                                                                                                                                               gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                   >  1:232185/1‑238 (MQ=255)
                                                                                                                                                                                                                               gcgaAACGTCGGCCGGGGGGGAGCGAGAGCGATGTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAgg                  <  2:822188/239‑1 (MQ=255)
                                                                                                                                                                                                                                cgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgc                 >  1:1188491/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gcAACGTGGGCCTGCGGTGAGCAATAGCAATGTCCCCTTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcg                <  1:733799/239‑1 (MQ=255)
                                                                                                                                                                                                                                   atgtccGGCCTGCGGTGAGCAAAAGCGTGTTCCCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  1:1225322/233‑1 (MQ=255)
                                                                                                                                                                                                                                   aaCGTCGCCCTGCGGTGAGCGATAGCGATGTCCCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  1:584196/238‑1 (MQ=255)
                                                                                                                                                                                                                                            cTGCGGTGGGGGATAGCGATGTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa     <  2:1221535/239‑1 (MQ=255)
                                                                                                                                                                                                                                            cTGCGGTGAGCGATGGCGATGTACCATTTCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa     <  1:373855/239‑1 (MQ=255)
                                                                                                                                                                                                                                               cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc   >  2:25405/1‑238 (MQ=255)
                                                                                                                                                                                                                                               cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc   >  2:934389/1‑238 (MQ=255)
                                                                                                                                                                                                                                                 gTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCg  >  1:825185/1‑237 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCG  >  NC_000913/989707‑990178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: