Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,304,798	T→C	18.8%	intergenic (+405/+310)	eco → / ← mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,304,798	0	T	C	18.8%	68.5 / 2.1	40	intergenic (+405/+310)	eco/mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (9/19);  new base C (2/6);  total (13/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGG  >  NC_000913/2304569‑2305033                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCCACATCCCACTTCCGACCCgg                                                                                                                                                                                                                                      >  1:772513/1‑239 (MQ=16)                              tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  1:1141769/239‑1 (MQ=255)                               ggggcgcgttAGCAGCCCTACAACGCTGTGAGGTGCTCCACCCCGTAGGTCGGATAAGGCGGTGACGCCGCACCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:1160670/230‑1 (MQ=11)                               ggCGCGTCTTATCAGCGCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:252816/238‑1 (MQ=18)                               ggCGCCTCTTATGAGGCCTACCCCGCTGTGAATGGCTCGACCCCGTAGGTCGGATAAGGCGGTTCCGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:928908/238‑1 (MQ=255)                                      cTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                                                                                                                 <  1:1028467/239‑1 (MQ=37)                                                  acacCGCTGTGAAGTGCTCCTCCCCGTAGGTCGGGTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTGGGTTGGATAAGGCGGTTACACCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                                                                                                                     <  1:62333/239‑1 (MQ=11)                                                      cGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAGGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  1:143084/215‑1 (MQ=21)                                                       gCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                <  1:391998/239‑1 (MQ=255)                                                        cTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCACTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  1:95899/213‑1 (MQ=21)                                                         tgtgAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCCACATCCAACGGCCGAGCCGGGTGTCTGCTGCGCCGCTGGCGCGCCCTTTCAGGCCTCCACCGCTCTGGAGTGCTcc                                                                                                                                                                              >  1:993376/1‑239 (MQ=11)                                                                         ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                                                                                                              <  1:1125731/239‑1 (MQ=255)                                                                          cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTTCGAAGTGCTCCACCCCGTAGGTTGGAAAAGGCGGGTAAGCCGCCTCCGACAACCAACGGCCGGACCCGTTGCCCGAGTCGACGCTCGCGCCGCCTTTAAGTCCTACACCGCCGTGAAGTGCTCCACCCCCTACGtgcgcga                                                                                                                                                             >  2:1212769/1‑233 (MQ=14)                                                                           gTAGGTCGTATACGGCGTTTACGCCGCATCCCACGTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                            <  2:671897/239‑1 (MQ=255)                                                                           gTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                            <  1:7004/239‑1 (MQ=255)                                                                                           ggTTACGCCGCATCCGACATCTAACGCCCACGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCAt                                                                                                                                           <  1:880387/239‑1 (MQ=255)                                                                                             ttACGCCGCATCCAGCAGCTAGCGCCCAAGCCGGTTGCCTGATGCGACTCTGGGGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGACGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATc                                                                                                                                          <  2:825730/238‑1 (MQ=11)                                                                                                          atcagcacaCGCCCCAGTGGGTTGGCGGAGGGGACGGTGGCGCGTCTTAACAGGACTAACCCGCTGTGAAGTGCGCCACCCCGTAGGGTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGcc                                                                                                                           <  2:1209021/231‑1 (MQ=11)                                                                                                                 aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCCGCA‑CCAACA‑TCTAACCCCCAAGccc                                                                                                                    >  1:229959/1‑238 (MQ=11)                                                                                                                  aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTGCTCCACCCCGTCGGTCGGCTAATGCGGTTGCG‑CCCCCCCCCACACTCTACCCCCCAGGCCg                                                                                                                    >  2:1004230/1‑239 (MQ=11)                                                                                                                                 tccttttGCGACGCTGGCGCGTCAGCTCAGGCCTCTGCCGCTGTGAAATGCTCCTCCCCGTAGGTCGGGTAACGCGGTTACGCCGCGTCCGACAGCCACCGCCCGAGCCGGTTGCCTGACGCGACGCGGGCTCGTCTTATCACGCCTACTCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCATGCCGGTTGCCTGATGCGACg                                                                                                    <  2:90722/233‑1 (MQ=11)                                                                                                                                                         tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                      <  1:406429/229‑1 (MQ=40)                                                                                                                                                         tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                            <  1:83218/239‑1 (MQ=40)                                                                                                                                                                 ctaacccGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCAGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                    <  2:439114/234‑1 (MQ=255)                                                                                                                                                                   acacCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCCACACCGCTGTGAAATGCTCCACCCCGTCGGTCGGATAAGGCGGGTAAC‑CAGCATTCGGCC‑TCCAAACCCCAAACTGGTTGCATGATACCGCTCTTGGGCGTATTCACAGGCCTACAccctgtcctt                                                                  >  2:406429/1‑231 (MQ=11)                                                                                                                                                                          tgtgACGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTc                                                            <  1:997423/238‑1 (MQ=255)                                                                                                                                                                                 tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                                                                                                                >  1:281541/1‑85 (MQ=255)                                                                                                                                                                                   cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                       >  1:417862/1‑108 (MQ=255)                                                                                                                                                                                   cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                       <  2:417862/108‑1 (MQ=255)                                                                                                                                                                                   cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCCATCGGCC‑TCTTACGCCCAAACCCGGTGCCCGTTGCGACGGCGGCGCGCCTTTTCAAGCCCACACCCCCGTGAAGTGGCCCCCCCCCTAg                                                  >  2:939994/1‑239 (MQ=11)                                                                                                                                                                                                     aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                    >  2:1200110/1‑109 (MQ=38)                                                                                                                                                                                                     aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                    <  1:1200110/109‑1 (MQ=38)                                                                                                                                                                                                         ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                                                                                                            <  1:939472/81‑1 (MQ=38)                                                                                                                                                                                                         ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                                                                                                            <  1:865610/81‑1 (MQ=38)                                                                                                                                                                                                         ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                                                                                                            >  2:939472/1‑81 (MQ=38)                                                                                                                                                                                                          gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTc                                                                                                                                                                               >  2:22849/1‑93 (MQ=37)                                                                                                                                                                                                              ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                        >  2:634183/1‑96 (MQ=35)                                                                                                                                                                                                              ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                        <  1:634183/96‑1 (MQ=35)                                                                                                                                                                                                                                ccAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTTTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGc     <  1:561920/239‑1 (MQ=17)                                                                                                                                                                                                                                   aCGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCgg  <  1:1212769/239‑1 (MQ=18)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACG‑CCGCATCCGACA‑TCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGG  >  NC_000913/2304569‑2305033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑