Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,367,368 G→T 17.7% D100Y (GAT→TAT)  arnC → undecaprenyl phosphate‑L‑Ara4FN transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,367,3680GT17.7% 137.0 / 3.2 58D100Y (GAT→TAT) arnCundecaprenyl phosphate‑L‑Ara4FN transferase
Reads supporting (aligned to +/- strand):  ref base G (10/37);  new base T (9/2);  total (19/39)
Fisher's exact test for biased strand distribution p-value = 3.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCTTACCGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGA  >  NC_000913/2367133‑2367606
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   
aaGCTTACCGGAATAAACCGGGCGCACACCCACAGCCTGGGAATCGTTGGGGAAAGAGTCTGAGAGCCTGCTGATTGATGACGCCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATg                                                                                                                                                                                                                                                  <  2:1158672/239‑1 (MQ=255)
        cGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTGTGACACGTTACTGGAGACTTTATTATTACACTTGATGCCggtgt                                                                                                                                                                                                                                           >  2:845536/1‑234 (MQ=255)
                  cAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGGAGGAGACCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGc                                                                                                                                                                                                                                <  2:533077/239‑1 (MQ=255)
                      cgcACCGCCACAGCCTGTGAATCGATGGGGAAAGAGTAGGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGcc                                                                                                                                                                                                                               <  2:672769/236‑1 (MQ=255)
                                      gtgAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCtg                                                                                                                                                                                                          <  1:1010417/239‑1 (MQ=255)
                                        gAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACCGTTTTTGCGGCTTAATTATTTTCCTTTATGCCCCTCTCCAGAATTCCCCCGAAAAAATCCCCCCCCcggt                                                                                                                                                                                                        >  1:444266/1‑239 (MQ=255)
                                            cGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCa                                                                                                                                                                                                    <  1:75532/239‑1 (MQ=255)
                                                     aaGAGTATGAGTCCCTGCTGTTTGTTGCCGCAAGTAGCGATAATCCCGCCCATAGGCTGGTCGAAGCCTCACAGGGGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGCCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGAt                                                                                                                                                                                           <  2:47417/239‑1 (MQ=255)
                                                        ggTATGAGAGCCGGCTTATTGACGCCGGCAGTTAGCAAAATTCCGGCGCATATGTTGTCCAAACCCCCAAAGGGGAAAAACAGCCATTTTGTTTCTATTTTGTTAAACCGAAATTCCGGGCAACATTCAGGGATTTGGGGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATg                                                                                                                                                                                          <  2:11831/236‑1 (MQ=255)
                                                        agTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTATTCACGTTACTGGGGACCTTATTATTATCCTTTGTGCCGCTCCTCCCAATCCGCCCAAAGAAAACCCCCCCCCGGTGGCTAAAAcccctgga                                                                                                                                                                                        >  2:1139454/1‑233 (MQ=255)
                                                               tacccgcttgattaatGCCCGCAGGTGAGATATTCCCGCGAATATCCTGTGCTAAGCCTCTAAAGCGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGAAACATTCAGCGATTATGGGGGGATTCAGTAACGTTACTGGCGACTTAATTATTACCCTGGATGCCGAGCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacg                                                                                                                                                                                 <  2:43185/231‑1 (MQ=255)
                                                                 tcttgctgATTGAGACCGGCAGTGGCGATAATCCCGCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  2:439051/236‑1 (MQ=255)
                                                                 tCCTGCTGATTGAGGACGGCAGTAGCAAAAATCCCGCGAATATCCTGGTGGAAGCCCCACAAGCGGAGACCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  2:306655/239‑1 (MQ=255)
                                                                 tCCTGCGTATGGAGGACGGCGGTGGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACGAGCGGAGAACAGCCATATTGTGTTTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTAACGTTACTGGCGACTTAATTATTGCCCTTGATGCCGATCTCCAGAAACCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  2:589613/239‑1 (MQ=255)
                                                                 gccTGCTGATTGATGACGGCAGTAGCAATAATTCCGCGCATATGCTGGTCAAAGCCCCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                                                                                                                                                                               <  2:778735/238‑1 (MQ=255)
                                                                  ccTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGCTTTAGTCACGTTATTGGCGGCTTAATTATTAACACTTATGCCGCTCTCCAGAAACAGCCCAAAGAAAAACCCCCCCCGTGGGGAAAAACCCACGAAAGTTacgccg                                                                                                                                                                              >  1:1140137/1‑239 (MQ=255)
                                                                    tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGTGTTTAATCACGTTACTTGCGACCTAATTTTTTCCCTTTTTGCCGGTCTCCCGAAACCACCCGAAAAAAAACCCCCCCCTGGGGGCAAAACCGATGAAAGGTacgacggg                                                                                                                                                                            >  2:918768/1‑237 (MQ=255)
                                                                    tgctgATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGGTACTGGCGACTTCATTATTACCCCTGATTGCCACCTCCAGGATCCCCCACAAGAAATCCCCCCCCTGGTGGCGAAAGCCGACGAAGGGTacaacggc                                                                                                                                                                            >  1:255111/1‑237 (MQ=255)
                                                                       tgattgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGt                                                                                                                                                                          <  1:82400/238‑1 (MQ=255)
                                                                         attgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACGTTACTGGCGACTTAATTTTTAACCTTTTTGCCGATCTCCCGAATCCGCCAGAAGAAAACCCCCGCCTGGTGGCAAAAGCCGATGAAGGGTacccactgggagg                                                                                                                                                                       >  1:59281/1‑228 (MQ=255)
                                                                          ttgatGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAgg                                                                                                                                                                       <  1:94127/238‑1 (MQ=255)
                                                                           tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCACGTTTCTGGCGGCTTTATTTTTAACCTTTATGGCGATCTTCAGAAAACGCCCGAAGAAATCCACCCCCTGGTGGCGAAAAACGATGAAGGTTACGACGGGGTAgggg                                                                                                                                                                     >  1:399787/1‑238 (MQ=255)
                                                                           tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                     <  1:512218/239‑1 (MQ=255)
                                                                           tgaggaCGGCAGTGGCGATAATTCCGCGCATATGCTGGTCGAGGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGa                                                                                                                                                                     <  2:618158/239‑1 (MQ=255)
                                                                                  ggCAGTAGCGATAATCCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACg                                                                                                                                                              <  1:641864/239‑1 (MQ=255)
                                                                                                                               aaGGGGAGAACAAGCATTTTTGTGTCTATTTGGCTTAACCCAAATTACGTGCAAGATTCAGCGATTATGGCGGGTTTCAGCCACGTTGCTGGCAACTTAATTTTTCCCCTTGATGCCGTACTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGaa                                                                                                                  <  2:930752/239‑1 (MQ=255)
                                                                                                                                       accAGCAATTTTGTTTCTATTTTGCTTAACCGCAATTCCGGGAAACATTCAGCGATTAGGGGGGGATTCAGCCACGTTACTGGCGATTTAATTATTCCCCTGGATGCCAATCTCCAGAATCCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                         <  2:59281/237‑1 (MQ=255)
                                                                                                                                        accatctattttGTGTCTATTTTGCTTAACCGAAATTCCGGGCAACATTCAGCGATTATGGGGGGATTCAGTCACGTTACTGGCGACTTAATTATTCCCCTTGATGCCGATCCCCAGAATCCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTaa                                                                                                         <  1:918768/229‑1 (MQ=255)
                                                                                                                                             cctttttGGGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGCCCCGTTACTGGCAACTTAATAATTCCCCTTGATGCCAATCCCAAGAACCCGCCAAAAGAAACCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGc                                                                                                    <  2:508671/233‑1 (MQ=255)
                                                                                                                                                ttttGTGTGTTTTTTGCTTAACCCAAATACCGGGCACCATTCAGCGATTAGGGCGGAATTCAGTCACGTTCCTGGCGACTTAATTATCCCCCTGGAGGCCGATCCCCAGAATCCCCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTa                                                                                                 <  2:399787/237‑1 (MQ=255)
                                                                                                                                                  ttGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCACGGTATAAGGGAATCAATTATTTATCTTTAAGCCGAGCTCCACAAACCCCCAGAAGGAATCACCCCCCCCGGGGGTAAAAACGAAGAAGGGTAAGCAGGGGGATGGACTGCACGCCAGAAACGCCAGGAAAGCGGGTTTCGTAACAACGCATTTGAAgaggataatcggcgttc                                                                                                 >  2:185516/1‑227 (MQ=255)
                                                                                                                                                       tCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTCACGTTACTTGCGGCTTTATTATTACCCTTTATGCCGATCTCCAGAATCCCCCAGAAGAAATCCCCCCCCTGGTGGCAAAAACCCATGAAAGTTACGACGTGGGAGGGACTGTACGCCCGAACCGCCCGGACAGCCGGTTTCGTAAAACCCCTTCGAAGATGATTAACCGGCTTATTCatc                                                                                           >  1:589919/1‑236 (MQ=255)
                                                                                                                                                       tCTATTTTCCTAACCCCCAATCGCGGCAACAATTAGCGGTTTTGGGAGGAATTCAGTCCCGTTTGGGTCGCCTTACTTCTTACCCTGTATCCCGTCCCCAAAAACCCCCCAGAAGAAACCCCCCCCCTGTTGGCAAAAGCAGATGAAGTTAACGACGTGTTAGGACCTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg                                                                                         <  2:298486/239‑1 (MQ=255)
                                                                                                                                                       gtgatttgacTTACCCGCAATAACGGGCAAGATACAGCATTTAGGGCGGGATTCAGTCACGTTACGGACGACTAATTGTTTCCCCTTGAGGCCAAGCCCCAGATTCCGCAAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTGCGACGTGGAAGGGACTGTACGCCAGATCCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAgcg                                                                                         <  2:255111/230‑1 (MQ=255)
                                                                                                                                                                 ttAAACGAAAATACGGGCAACATACATCGATTATGCGGGGATTCGTTCCCTTTGCTGGTGCTTTAATTTTTCCCGTGGAGTCCGATCTCCAGAACCCGCGAAAAAAAAGCCCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGATGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGc                                                                               <  2:444266/239‑1 (MQ=255)
                                                                                                                                                                   aaCCGCAATTACGGGCAACATTCAGCGATTATGGCGGTGTTTAGTCCCGTTACTTGCGACCTAATTATTATCCTTTTTGCCGATCTCCCGAATCCGCCAGCAGAAAACCCCCCCCTGGTGGCCCAAGCCGGCGAAAGTTACGACGTGGGTGGGACGGTTCGGCAGAAACCCCCAGGCCGCCGGGTTCCTTAAACCCCTCCCGAGAAGAGTAAACCGCTTATTCATGCGcacaacggcac                                                                              >  2:333737/1‑234 (MQ=255)
                                                                                                                                                                       gCAATTCCGGGCACATTTCAGGGATTATGGCGGGATCCAGCAACGTTCCTGGCAACTAAATTATTCCCCTTGAGGCCGATCCCCAAAACCCGCCAGAAAAATCCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTAGGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCg                                                                         <  2:522021/239‑1 (MQ=255)
                                                                                                                                                                           ttACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATgg                                                                     <  1:583008/239‑1 (MQ=255)
                                                                                                                                                                                 caacaagtCAGCGTTTAGGGGGGGATCCAGTCAGTTTACTGGCGACTTAATTATTCCCCTGGAGGCCGATCCCCAGAACCCCCCAGAAAAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGAt                                                                <  2:538376/231‑1 (MQ=255)
                                                                                                                                                                                  aaaCAGTCAGGGATTAGGGGGGAAGCCAGCCGCGTTGTGGGCGACATAATTATTCCCCTGGTTGCCGAGCTCAGGAACCCGCCAGAAGAAATCCCCCGCCTGGTGGAAAAAGCCGAGGAAGGTTACGACGTGGTAGGGCCGGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                              <  2:987187/238‑1 (MQ=255)
                                                                                                                                                                                    aaaTTCAGCGTTTATGGGGGGTTTCAGCAACGTTCCTGGGGATTAATTTTTTCCCCTGGAGGCCGATCCCAAGAATCCCCAGGAAGAATCCCCCCGCCGGGGGGCAAAACCCGATGAGGGTTACGAGGTGGTAGGGACTGTCCGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACg                                                            <  2:589919/237‑1 (MQ=255)
                                                                                                                                                                                             ggttAGGGCGGAATTCAGCCGTGTTCGTGCGGCTTAAATTATACCCTTAGAGGCCGATCTCCAAAACCCTCCAAAAGAAATCCCCCGCCGGGTGGAAAAAGCCGATGAAGGTTACGACGTGGTGGGGCCTGTACGCCAGACCCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTa                                                      <  2:1206403/234‑1 (MQ=255)
                                                                                                                                                                                                tATGGCGGGATTCAGTCACGTTCCTGGCGACTTAATTTTTCCCCTTGATCCCGATCTCCAGAAACCGCCAGAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTGGGGATTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTAtgctgc                                                <  1:928314/239‑1 (MQ=255)
                                                                                                                                                                                                          aTTCAGTCACGTGACGGGAGATTTAATTATGCCCCTGTGTGCAGATGTCCAGAACCCGCCAGAAGAAATCCCCCGCCTGGGGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTAt                                        <  2:1070730/238‑1 (MQ=255)
                                                                                                                                                                                                                   cGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAACCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCata                              <  1:1139454/239‑1 (MQ=255)
                                                                                                                                                                                                                    gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                              <  1:720222/206‑1 (MQ=255)
                                                                                                                                                                                                                    gTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTa                                                              >  2:720222/1‑206 (MQ=255)
                                                                                                                                                                                                                        cTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTc                         >  1:297502/1‑239 (MQ=255)
                                                                                                                                                                                                                         tGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcg                        >  2:460712/1‑239 (MQ=255)
                                                                                                                                                                                                                          ggCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAGGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGGAGGGACGGTACGCCAGAACCGCCAGGACAGGTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCTCACCACTGGCAAAGCGATGGGTGATTACGGTGGTATTCTTCGCGCCTCTCGCCGTCATATTGTcgt                       >  2:236727/1‑238 (MQ=255)
                                                                                                                                                                                                                             gACTTAATTATTCCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatg                     <  1:845536/238‑1 (MQ=255)
                                                                                                                                                                                                                              aCTTATTTATTCCCCTGGATGCCGATCCCCAAAAGCGGCAGAAAGAAACCCCCCGCCTGGTGGCAAAAGCCGATGAAGTTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                   <  2:297560/239‑1 (MQ=255)
                                                                                                                                                                                                                              aCTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCCTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcg                   >  1:872031/1‑239 (MQ=255)
                                                                                                                                                                                                                                ttAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTcgatgcgat                 >  1:475296/1‑239 (MQ=255)
                                                                                                                                                                                                                                      attcCCCTTGATGCCGATCTCCAAAATCCCCGAGAAGAAATCCCCCGCCTGGTGCCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACGGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCa           <  1:185516/239‑1 (MQ=255)
                                                                                                                                                                                                                                        tccccGTGATGCCAATCGCCGAAACCCGCCAAAAAAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt         <  1:518576/237‑1 (MQ=255)
                                                                                                                                                                                                                                         aCCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt         >  1:158310/1‑238 (MQ=255)
                                                                                                                                                                                                                                            cTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCa     >  1:896004/1‑239 (MQ=255)
                                                                                                                                                                                                                                                gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  2:1038544/1‑239 (MQ=255)
                                                                                                                                                                                                                                                gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  1:651384/1‑239 (MQ=255)
                                                                                                                                                                                                                                                gATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGa  >  1:651234/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   
AAGCTTACCGGAATTAATCAGGCGCACCACCACAGCCTGTGAATCGTTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAGAATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGA  >  NC_000913/2367133‑2367606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: