Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,937,136 IS2 (–) +5 bp coding (485‑489/870 nt) yebK → putative DNA‑binding transcriptional regulator

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1651548NA (NA)48 (1.120) 42/284 0.1 100% noncoding (706/706 nt) IS2 repeat region
?NC_000913 = 1937140 0 (0.000)coding (489/870 nt) yebK putative DNA‑binding transcriptional regulator
* ? NC_000913 1937136 =0 (0.000)59 (1.380) 50/284 0.0 100% coding (485/870 nt) yebK putative DNA‑binding transcriptional regulator
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region

CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCT  >  NC_000913/1651409‑1651682
                                                                                                                                           |                                                                                                                                      
cTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGGTGGGTTATCGCTCGTCATTGGATTATCTGCGGCAGCGGGCTTGTAATGGGTGAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAg                                                                                                                                     <  1:68008/143‑1 (MQ=11)
  tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATGTCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGACATATAGGGGCAAATCCACAGGt                                                                                                                                   >  2:503841/1‑143 (MQ=17)
         gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTATGGAAATATAGCGGAAAATACACAGGTGAAATaa                                                                                                                            >  1:634949/1‑142 (MQ=11)
          cGTGCGCGCATTTGATCGCATGGCGTCCGCAGCGTGCGCGGGGTTATCGCTCGCCTCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGCGATACCAAATGCCTGGAAATAGAGGGGCAAATCCACAGGTGAACTACg                                                                                                                           <  2:562867/143‑1 (MQ=14)
           gTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGc                                                                                                                          >  2:53004/1‑143 (MQ=40)
                 gCACTATAACGCACGGCATCCCCATAGTGCGCTGGGTTATCGATAGTAACCGGAATATCTGCGGCAGCCGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCctct                                                                                                                    <  1:29474/143‑1 (MQ=14)
                    ttATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCCCtgtt                                                                                                                 >  2:591810/1‑143 (MQ=255)
                          cGAATTGCATCCGCAAAGTGCGCCAGGATATCGCACGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTTTTAc                                                                                                           <  1:182128/143‑1 (MQ=17)
                          cGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTCAGTGATAACAGATGTCTGGAAATAGAGGGGCAAATCCACAGGTGAACTACGCTCCTCtgttggtgag                                                                                                           >  2:778728/1‑140 (MQ=255)
                          cGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTAc                                                                                                           >  2:396888/1‑143 (MQ=255)
                           gaagcgCAGCCGCATAGTGCGCTGGGTTATCGCTCGCGACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACg                                                                                                          <  2:575487/138‑1 (MQ=255)
                           gAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACg                                                                                                          <  2:178229/143‑1 (MQ=255)
                                 cacccGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaa                                                                                                    <  1:644990/140‑1 (MQ=255)
                                  aTCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGTTGAACTACGCTTCTCTGTTTGTTTCGccttatc                                                                                                   >  1:167553/1‑137 (MQ=14)
                                    gcGCATGGTGCGCTGGGTTATCGCCCGCCACGGGCATATCTGCGGCCGCGGGCTTGTCATGGGTTACGTCATAACCGATGTCTGGAAATATAGGGCCCAATCCACAGGTCAACTACGCTCCTCTGTTTGTTACGCAATaaaaa                                                                                                 <  2:724813/142‑1 (MQ=18)
                                    ccGCATAGTGCGCTGGGGTGTCGCTCGCCACGGGAATATCTGCGGCCGCGGGCTTGTAATGGGTCAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATaaaaa                                                                                                 <  2:630596/143‑1 (MQ=255)
                                     cgcgTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGGTGTCTGGAAATATAGTGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAAc                                                                                                <  2:178390/143‑1 (MQ=255)
                                              cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGAGAACAGAGGTCTGGCAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGtttt                                                                                       >  1:213180/1‑143 (MQ=255)
                                                 tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGa                                                                                    >  2:582660/1‑143 (MQ=255)
                                                         cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCaa                                                                            >  2:149123/1‑143 (MQ=255)
                                                          gctcggCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCaaa                                                                           <  2:455104/143‑1 (MQ=255)
                                                          gctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCGGGCGATGTAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATGAAAACTGGTGTTTGCGACTATACaac                                                                           >  2:722888/1‑142 (MQ=21)
                                                                 aCGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGtct                                                                    >  2:242051/1‑141 (MQ=255)
                                                                                gCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAAt                                                     >  1:9821/1‑143 (MQ=255)
                                                                                        cTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaaaa                                             >  1:338296/1‑143 (MQ=255)
                                                                                         ttGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGaaaaa                                            >  1:638099/1‑143 (MQ=255)
                                                                                             aaTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAATTCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAATCTGGTGTTGTCGACTATGCAAAACAGTTTCCCTCCTCACACAATCTTGAAAAATTTg                                        <  2:92074/143‑1 (MQ=38)
                                                                                                   gtAAGGGAGAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGGTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAgg                                  <  2:729234/142‑1 (MQ=255)
                                                                                                      aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGccc                               >  2:121253/1‑143 (MQ=255)
                                                                                                        tGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAACACTCCGTGCGGCCCGa                             >  2:300642/1‑143 (MQ=39)
                                                                                                           tAAGAGGTGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACaa                          <  2:69172/143‑1 (MQ=255)
                                                                                                               aGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGagt                      >  2:707799/1‑141 (MQ=255)
                                                                                                                   ttCTTGAAGTTTATGGGTAAATCCACAGTTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTTGTGTTTGCGACTATGCAAAACAGTTTCCCTCCTCACTCAATCTGGAAAAATTTTGTGAGGCCCGACAAGATTTAAc                  <  2:634949/142‑1 (MQ=255)
                                                                                                                    tCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAAcc                 >  2:300603/1‑143 (MQ=255)
                                                                                                                        gAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATCAAAACTGGTGTTTTCGCCTATGCAAAACAGTTTCACTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATaa             >  2:528967/1‑143 (MQ=255)
                                                                                                                         aaaTTTAGGGGCAAGTCCACAGGCGACCTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATaaa            <  2:87348/143‑1 (MQ=255)
                                                                                                                         aaaTATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAACATTTGGTGAGGCCCGACAAGACTTAACCATaaa            >  1:584806/1‑143 (MQ=255)
                                                                                                                          aaTATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATaaaa           <  2:269910/143‑1 (MQ=255)
                                                                                                                                   gCAAATCCACAGGTGAACTACGCTACTCTTTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCt  >  2:278914/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACAGGTGAACTACGCTCCTCTGTTTGTTACGCAATAAAAACTGGTGTTTTCGACTATGCAAAACAGTTTCCCTCCTCACGCAATCTGGAAAAATTTGGTGAGGCCCGACAAGATTTAACCATAAAAGAACTGGCT  >  NC_000913/1651409‑1651682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.