Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	1 (0.020)	38 (0.890)	35/284	0.2	97.4%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	1 (0.020)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTA  >  NC_000913/4001515‑4001786                                                                                                                                  |                                                                                                                                               tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCACCGGCCCGCTGGTTTGACGACGACGACGGCCTGCATctccttcttcttc                                                                                                                                   >  2:161688‑M1/1‑130 (MQ=255)   ttGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctt                                                                                                                                 >  1:725973‑M1/1‑128 (MQ=255)    tGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttcttt                                                                                                                                <  1:206760‑M1/143‑17 (MQ=255)     gtctgaccggaCGACGACGAGCGACCGCGCGTACAATCTGAACTTGTCCAGAGCCTTGCAACCAGCCCGGAACTGGAGGACATCGATGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttg                                                                                                                               <  2:404441‑M1/138‑18 (MQ=255)     gTCGAACCGGACGCCGACGCGCGACTGTGAATAGTGTCCGAACTTGGCCTTTGCCGGCCAACCCGCCCGGAACTGGTAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTg                                                                                                                          <  2:718447/143‑1 (MQ=255)     gTCGAACCGCACGACGACGAGCGCGTGCGCGCACCATCTAAAGCACGCCTATGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttg                                                                                                                               <  2:673568‑M1/143‑18 (MQ=255)      tCGAACCGGACGACGACGAGCGCCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttga                                                                                                                              <  1:46409‑M1/143‑19 (MQ=255)       cGAAGCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaa                                                                                                                             <  2:613319‑M1/143‑20 (MQ=255)       cGAACCGGACGACCACGAGCGACTGCGCGTACAATCTGAACGTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaa                                                                                                                             <  1:86115‑M1/143‑20 (MQ=255)              gacgactacGTGCGATCGCTCGTGCGATCGGAGCTTGGCCAGAGCCTGGCAACCCGCGCGGAACTGGAAGACATAGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcgg                                                                                                                      <  2:416145‑M1/143‑27 (MQ=255)                  acggcgaGCGACTGCGCGTACAATCTGCACTTAGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttctgctttgaagatgcggaaga                                                                                                                  <  2:543622‑M1/143‑31 (MQ=255)                   cgacgaGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaaggt                                                                                                                 >  1:224005‑M1/1‑112 (MQ=255)                         gCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACTATGACGGCTTGCATctatttcttattattagaagatgaagaaaaacacgcc                                                                                                           >  1:70573‑M1/1‑106 (MQ=255)                           gACTGCGCGTACAATCTGACCTTGGCCGCAGGCTGGCACCCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccgg                                                                                                         <  1:486930‑M1/143‑40 (MQ=255)                            aCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgccgacgctcacgccggt                                                                                                        >  1:489136‑M1/1‑103 (MQ=255)                              tGCGCGGAGAATCGGGAGTTGGCCAGAGCCGGGCGACCCGCCCGGAACGGGAAGAGATCGAAGCGTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAGctccgtcttcttctttgaagatgcggaagatcacgccggtaa                                                                                                      <  2:9747‑M1/143‑43 (MQ=255)                                cgcgTACAATCTGCACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttcttttaagatgcggaagatcacgccggtaact                                                                                                    <  2:234252‑M1/143‑45 (MQ=255)                                     aCAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccact                                                                                               <  1:193603‑M1/143‑50 (MQ=255)                                        aTCTGAACTTGCCCAGAGCCTGGCAACCCGCCCGGAACCGGAAGACATCGAAGCATCGGCACGTTTCTGTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtg                                                                                            <  1:260513‑M1/143‑53 (MQ=255)                                         tCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctcctgcttcttctttgaagatgcggaagatcacgccggtaactccactgcgc                                                                                           >  1:583181‑M1/1‑90 (MQ=255)                                               cTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcattta                                                                                     <  2:232867‑M1/143‑60 (MQ=255)                                                    ccAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatc                                                                                >  1:650141‑M1/1‑79 (MQ=255)                                                         gCCTGGCACCCCGCCCGGAACTGGAAGGCATCGAACCATCGGCTCGTTTCTTTGAAGACGACGTCGGCCTGCACctccttcttcttctttgcagatgcggcagatcacgccggtaactccactgtggcatttaccatccgtga                                                                           <  2:396293‑M1/143‑70 (MQ=255)                                                           cTGACAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTGTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactttggcatttaccatccgtgatg                                                                         <  1:337580‑M1/143‑72 (MQ=255)                                                                  cccgcccgGAACTGGAAGCCCTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtct                                                                  <  2:168040‑M1/143‑79 (MQ=255)                                                                   ccgcccgGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatctaccatccgtgctggcggtaga                                                                 >  2:265204‑M1/1‑64 (MQ=255)                                                                           aaCTGGAAGACATCGAAGCATCTGCACGTTTCTTTGAAGACAACGACGGCCTGCATccccttcctcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactct                                                         <  1:144802‑M1/143‑88 (MQ=255)                                                                             cTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgc                                                       <  2:177700‑M1/143‑90 (MQ=255)                                                                              tGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccaccatgctgcgcatcatccgtgatggtcgtctgtttactctgcg                                                      >  2:342079‑M1/1‑53 (MQ=255)                                                                                  aGACATCGATGCATCGTCACGGTGCTGTGAAGACGACGACGGCCTGCATctgcttcttcttctttgaagatgcggcagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgag                                                  <  2:344583‑M1/143‑95 (MQ=255)                                                                                          aaGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaact                                          <  2:650141‑M1/143‑103 (MQ=255)                                                                                               tCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccg                                     >  1:146954‑M1/1‑36 (MQ=255)                                                                                                          cTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttcctctgcttgagcgtgaactgcccgcttttcgtctg                          >  1:343837‑M1/1‑25 (MQ=255)                                                                                                          cTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctg                          <  2:519658‑M1/143‑119 (MQ=255)                                                                                                                   cgacgacgGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatg                 <  1:339333‑M1/143‑128 (MQ=255)                                                                                                                    tacgatggCCTGCATctgcttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgc                <  1:94483‑M1/142‑129 (MQ=255)                                                                                                                    gacgacgGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgc                >  1:716243‑M1/1‑15 (MQ=255)                                                                                                                    gacgacgGCCTGCATctccttcttcttcgtggaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgc                <  1:640730‑M1/143‑129 (MQ=255)                                                                                                                                  tctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttcccatccgtgagggtcgtccgtttactctgcgtgaccgtgaactgcccggttttcgtctgtaccgtaggcgtgcccgcggccag  >  2:690762‑M1/1‑1 (MQ=255)                                                                                                                                  |                                                                                                                                               TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTA  >  NC_000913/4001515‑4001786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.