New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4107580 =	56 (1.310)	4 (0.100)	3/272	6.4	7.2%	coding (29/963 nt)	pfkA	6‑phosphofructokinase I
	?	NC_000913	4107611 =	50 (1.220)					coding (60/963 nt)	pfkA	6‑phosphofructokinase I
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAG  >  NC_000913/4107438‑4107721                                                                                                                                               |                                                                                                                                              cacttCAGCAGGAAGTGATTGTTATACTACTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTGCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAg                                                                                                                                               <  1:272518/140‑1 (MQ=255)     tCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAgcggc                                                                                                                                           <  2:347418/143‑1 (MQ=255)        gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGt                                                                                                                                        >  1:252763/1‑143 (MQ=255)         cagGAAGTGATTGTTATACGATTTGCACCTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTg                                                                                                                                       <  2:435138/143‑1 (MQ=255)          agGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGGGa                                                                                                                                      >  1:654675/1‑143 (MQ=255)            gAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGCCAAGCGGCGGTGATg                                                                                                                                    >  2:202704/1‑143 (MQ=255)                         taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGaa                                                                                                                       <  2:489070/143‑1 (MQ=255)                              tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGCTGCGCCTGGCGTGATcgccg                                                                                                                  >  1:249872/1‑143 (MQ=255)                               ttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAAcgccgc                                                                                                                 <  1:559170/143‑1 (MQ=255)                                 gCACATTCGTTGGATCACTTCGATGTGCAAGACGACTTCCCGCAACAGATTTCATTTTGCATTCCAAAGTTCCGAGGTAGTCATGATTAAGAAAATCGCTACGTTGACAAGCGACGGTGATGCGCCAGGCAGCAACGCCGCac                                                                                                               >  2:563919/1‑142 (MQ=255)                                        cGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCgg                                                                                                        <  1:475733/143‑1 (MQ=255)                                             gATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGgttg                                                                                                   >  1:563327/1‑143 (MQ=255)                                               tCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCGTGATTAAGAAAATCGGTGAGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCggggaggtt                                                                                                 >  2:510935/1‑138 (MQ=255)                                                cACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTgttc                                                                                                <  1:172251/143‑1 (MQ=255)                                                   ttCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGCTTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGGACTTCGCGGGGTTgttcgtt                                                                                             >  2:397383/1‑143 (MQ=255)                                                      gATGGGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTGGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTg                                                                                          <  1:218920/143‑1 (MQ=255)                                                       atctgCAAGAAGACTGCCGGCAACAGATTTCATGTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTgc                                                                                         <  2:373305/140‑1 (MQ=255)                                                        tgtgCAAGAAGCCTTCCGGCTACAGATGTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTgcg                                                                                        <  2:610223/143‑1 (MQ=255)                                                         gtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTgcgc                                                                                       >  1:743480/1‑143 (MQ=255)                                                          tgCAAGAAGACTTCCGGCAAAAGATTTCATTTTGCATTCAAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCt                                                                                      <  1:636994/143‑1 (MQ=255)                                                           gCAAGGAGACTTCCGGCAAGAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTg                                                                                     <  2:508992/143‑1 (MQ=255)                                                            cAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGa                                                                                    <  1:458607/143‑1 (MQ=255)                                                                     ttCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCACTTCGCGGGGTTGTTCGTTCTGCGCTGCCAGAAgtcc                                                                           >  1:65906/1‑140 (MQ=255)                                                                     ttCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTGTGCGCTGCCAGCAgggc                                                                           >  1:459762/1‑141 (MQ=255)                                                                      tCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCTGAGGTAGTCATGATTAAGAAATTCGTTGTTTTGACAAGCGGCGGTGATGCGCCAGGCAGGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCt                                                                          <  1:4677/143‑1 (MQ=255)                                                                      tCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCt                                                                          <  1:509117/143‑1 (MQ=255)                                                                        cGGCAACAGATTGCATTTTGCAGTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTCGTCAGGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTgg                                                                        <  1:657764/143‑1 (MQ=255)                                                                          gCAACAGATTTCATTTTGCATTCCATAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGaa                                                                      <  2:182960/143‑1 (MQ=255)                                                                          gCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGaa                                                                      >  1:28079/1‑143 (MQ=255)                                                                               aGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAGTTGGGGGGGACGCACGTTCTGCGCTGACAGAAGGTCTGGAAGTAAt                                                                 >  2:757532/1‑143 (MQ=255)                                                                                    gcATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTa                                                            <  2:331274/142‑1 (MQ=255)                                                                                      aTTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCGAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGGAtt                                                          >  2:710079/1‑143 (MQ=255)                                                                                      aTTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTgttcgtt                                                                                             >  2:123795/1‑108 (MQ=255)                                                                                      aTTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTgttcgtt                                                                                             >  2:125233/1‑108 (MQ=255)                                                                                      aTTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTgttcgtt                                                                                             <  1:123795/108‑1 (MQ=255)                                                                                      aTTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTgttcgtt                                                                                             <  1:125233/108‑1 (MQ=255)                                                                                        tttGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTa                                                        >  1:449860/1‑143 (MQ=255)                                                                                        tgtGCATTCCAAAGTTTAGAGGTAGGCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTa                                                        <  2:530109/141‑1 (MQ=255)                                                                                         ttGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTAt                                                       <  1:711873/143‑1 (MQ=255)                                                                                           gCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAACATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTGGTTCCAAGTGAGATGACACAAGGTCTGGAAGTAATGGGTATTTATGa                                                     >  1:324264/1‑143 (MQ=255)                                                                                              tgccAAAGTTCAGAGGTAGTAATGATTACGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACgg                                                  <  2:380507/141‑1 (MQ=255)                                                                                                 cAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGATCGCTGCTATTCGCGGCCAACTTCGTTCTGCGCTGACAGCAGGTCTGGAAGTAATGGGGATTTATGGCGGCTa                                               >  1:359763/1‑143 (MQ=255)                                                                                                 cAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGCAGGTCTGGAAGTAAGGGGGATTTGTCACGGCtg                                               >  1:437038/1‑142 (MQ=255)                                                                                                   aaGTTCATAGGTAGTCATGATTAAGAACATCGGTGTGTTGCCAAGCGGCGGTGATGCGCCAGGCATGACCGCCGCAAGTCGCGGGGTTGTTCGTTCTGCGCTGTCAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATc                                             <  2:214938/143‑1 (MQ=255)                                                                                                      tgcAGATGTAGTCAGGAATAAGAAAGTCGGTGTCGTGACAAGCGGCGGGGATGCGCCAGGCATGAACGCCGTAATTCGCGGGGTTGTTCGTGCTGCGCTGACAGAATGTCTGGAAGTAATGGGTATTTATGACGGCTATCTgg                                          <  2:303674/141‑1 (MQ=255)                                                                                                        cAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGt                                        <  1:420448/143‑1 (MQ=255)                                                                                                          gagGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTACTGGGTATTTATGACGGCTGTCTGGGTcc                                      >  1:120273/1‑142 (MQ=255)                                                                                                                  cATGATTAAGAAAATCGGTGTTTTGACAAGTGGCGGTGATGCTCCTGTCATGAACGCCGCAATTCGCGTGGTTGTTCGTTCTGCGCTGACTGAAGATCTCTAAGTAATTGGTATTTATGACGGCTATCTGGGTCTGTATGAAg                              <  2:54702/143‑1 (MQ=255)                                                                                                                  cATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAAAGCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGACTGGAAGTAGTGGGTATTTCTGACGGCTATCGGGGTCTGTCTGAAg                              >  2:540737/1‑143 (MQ=255)                                                                                                                   aTGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGCCAGGAGGTCTGGCAGCACTGGGTATTTATGCCGGCCATCTGGGTCTGTATGCAgg                             >  1:603860/1‑142 (MQ=255)                                                                                                                         aaGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTAt                       >  1:474577/1‑143 (MQ=255)                                                                                                                         aaGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTAt                       <  1:743557/143‑1 (MQ=255)                                                                                                                                tCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTAtt                                                          <  2:608630/101‑1 (MQ=255)                                                                                                                                tCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTAtt                                                          >  1:608630/1‑101 (MQ=255)                                                                                                                                       gtTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGAcc         >  1:488943/1‑143 (MQ=255)                                                                                                                                              aGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGGATTTATGACGGCTCTCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGCGACAg  >  2:511510/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                              CAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACCGTATGGTACAGCTAGACCGTTACAG  >  NC_000913/4107438‑4107721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.